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The map label for this gene is glnA2 [H]

Identifier: 68535750

GI number: 68535750

Start: 811395

End: 812741

Strand: Direct

Name: glnA2 [H]

Synonym: jk0675

Alternate gene names: 68535750

Gene position: 811395-812741 (Clockwise)

Preceding gene: 68535748

Following gene: 68535751

Centisome position: 32.95

GC content: 62.44

Gene sequence:

>1347_bases
ATGGGGGACATGAATCGCCAACAGGAATATGTTCTGCGCACGCTGGAAGAACGCGACATCCGCTTCGTTCGCCTTTGGTT
CACGGATATCCAGGGGTATCTCAAGTCCGTGGCGGTGGTGCCAGCGGAACTAGAGGGGGCTTTCCAGGAGGGCATCGGTT
TCGACGGCTCGGCGATTGAAGGTTTTTCCCGCATCGCAGAGTCGGACACGATTGCGCAGCCGGACCCGTCGACGTTCCAG
ATCTTCCCGTTTAAATCGGATGATGGAGCTTTGTCGGCGCGCATGTTCTGCGACATTGCGATGCCGGATGGCACCCCTTC
GTGGGCGGATCCGCGCCAGGTGCTGCGCCGCCAGATGAAGGCCGCGGCGGACCAGGGGTTCAGCTGCTACGTCCACCCGG
AGATCGAGTTTTTCCTGGTTAAGGAACTGGAGACGGAGGGTTATCCGCCGGTGCCGACGGATAACGGGGGATACTTCGAC
CAGGCGGTCACCGACGACGCGCCTGCCTTCCGCGCCCACGCTATTAGCGCGCTGGAGCATATGGGTATTTCGGTGGAGTT
CTCTCACCACGAGAACGCGCCGGGGCAGCAGGAGATCGACCTGCGCTACGCCGACGCCCTGACGATGGCGGATAACGTGA
TGACCTTCCGCTACCTGATTAAGCAGGTCGCCCGCCGCAACGGGGTGGCCGCGACGTTTATGCCTAAGCCTTTCCATGAG
CACGCGGGTTCGGCGATGCACACGCACATGTCCCTGTTCGAGGGGGAGAATAACGCTTTCCACGACCCGGATGATGAGCT
GCGCCTGTCGGGCACCGCGAAGTCCTTCATCGCGGGCATTCTGGAGCATGCCCCGGAGATCACGGCGGTGACGAACCAGT
GGGTGAACTCCTACAAGCGCATTACCTCCGGCGATGAGGCTCCGCGTGCGGCGGCGTGGGGTGCGTCGAACCGCTCCGCG
ATGGTGCGCCTGCCGATGTACACGCAGAACAAGGCGGCTTCCCGCCGCGTGGAGGTCCGCTCGCCGGATAGCGCCTGCAA
CCCCTACCTGGCTTATTCGGTGCTGCTGGCCGCAGGTTTGAAGGGCATCGAGGAGGGCTACGAGCTGCCGAACCCGGCGG
AGGAGGATGTCTCCCGTATGACCCGCCGCGAGCGCATTGCCGCAGGCTATAAGGATCTGCCCTCGGATCTGAACAGCGCC
CTGCGCGAGATGGAGCGTTCCGAGCTAGTTGCCGATGCCCTCGGCGAGCACGTATTTGAGTTCTTCCTACGAAACAAGTG
GCGCGAGTGGTACGAGTACCAGTCGCAGATCACGCCGTGGGAGCTGCGGAACAACCTGTCCTTCTAG

Upstream 100 bases:

>100_bases
AGGTGACGCCCTCAGCCCCAACCGCTCAACCTTCGACTAGTGCAAACGGGGGCGCAGCCTAAGCGCAAACGGGGGCGCAG
CCGGGCAGGGAACTTCTACA

Downstream 100 bases:

>100_bases
CCGACTTTTCCTGCGGCTTCCCCCTCCCGTACTTCCAACACCTTCAGTACTTCCTAGAGTTCCAGGAGCACTTTCACCCC
ATGACCCGCCCCCGCAGTTC

Product: hypothetical protein

Products: NA

Alternate protein names: Glutamate--ammonia ligase 2 [H]

Number of amino acids: Translated: 448; Mature: 447

Protein sequence:

>448_residues
MGDMNRQQEYVLRTLEERDIRFVRLWFTDIQGYLKSVAVVPAELEGAFQEGIGFDGSAIEGFSRIAESDTIAQPDPSTFQ
IFPFKSDDGALSARMFCDIAMPDGTPSWADPRQVLRRQMKAAADQGFSCYVHPEIEFFLVKELETEGYPPVPTDNGGYFD
QAVTDDAPAFRAHAISALEHMGISVEFSHHENAPGQQEIDLRYADALTMADNVMTFRYLIKQVARRNGVAATFMPKPFHE
HAGSAMHTHMSLFEGENNAFHDPDDELRLSGTAKSFIAGILEHAPEITAVTNQWVNSYKRITSGDEAPRAAAWGASNRSA
MVRLPMYTQNKAASRRVEVRSPDSACNPYLAYSVLLAAGLKGIEEGYELPNPAEEDVSRMTRRERIAAGYKDLPSDLNSA
LREMERSELVADALGEHVFEFFLRNKWREWYEYQSQITPWELRNNLSF

Sequences:

>Translated_448_residues
MGDMNRQQEYVLRTLEERDIRFVRLWFTDIQGYLKSVAVVPAELEGAFQEGIGFDGSAIEGFSRIAESDTIAQPDPSTFQ
IFPFKSDDGALSARMFCDIAMPDGTPSWADPRQVLRRQMKAAADQGFSCYVHPEIEFFLVKELETEGYPPVPTDNGGYFD
QAVTDDAPAFRAHAISALEHMGISVEFSHHENAPGQQEIDLRYADALTMADNVMTFRYLIKQVARRNGVAATFMPKPFHE
HAGSAMHTHMSLFEGENNAFHDPDDELRLSGTAKSFIAGILEHAPEITAVTNQWVNSYKRITSGDEAPRAAAWGASNRSA
MVRLPMYTQNKAASRRVEVRSPDSACNPYLAYSVLLAAGLKGIEEGYELPNPAEEDVSRMTRRERIAAGYKDLPSDLNSA
LREMERSELVADALGEHVFEFFLRNKWREWYEYQSQITPWELRNNLSF
>Mature_447_residues
GDMNRQQEYVLRTLEERDIRFVRLWFTDIQGYLKSVAVVPAELEGAFQEGIGFDGSAIEGFSRIAESDTIAQPDPSTFQI
FPFKSDDGALSARMFCDIAMPDGTPSWADPRQVLRRQMKAAADQGFSCYVHPEIEFFLVKELETEGYPPVPTDNGGYFDQ
AVTDDAPAFRAHAISALEHMGISVEFSHHENAPGQQEIDLRYADALTMADNVMTFRYLIKQVARRNGVAATFMPKPFHEH
AGSAMHTHMSLFEGENNAFHDPDDELRLSGTAKSFIAGILEHAPEITAVTNQWVNSYKRITSGDEAPRAAAWGASNRSAM
VRLPMYTQNKAASRRVEVRSPDSACNPYLAYSVLLAAGLKGIEEGYELPNPAEEDVSRMTRRERIAAGYKDLPSDLNSAL
REMERSELVADALGEHVFEFFLRNKWREWYEYQSQITPWELRNNLSF

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=467, Percent_Identity=23.3404710920771, Blast_Score=114, Evalue=1e-25,
Organism=Escherichia coli, GI1790301, Length=388, Percent_Identity=38.4020618556701, Blast_Score=221, Evalue=9e-59,
Organism=Escherichia coli, GI87081870, Length=451, Percent_Identity=27.0509977827051, Blast_Score=134, Evalue=8e-33,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 50462; Mature: 50331

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGDMNRQQEYVLRTLEERDIRFVRLWFTDIQGYLKSVAVVPAELEGAFQEGIGFDGSAIE
CCCCCHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHH
GFSRIAESDTIAQPDPSTFQIFPFKSDDGALSARMFCDIAMPDGTPSWADPRQVLRRQMK
HHHHHHCCCCCCCCCCCCEEEEEEECCCCCEEEEEEEEEECCCCCCCCCCHHHHHHHHHH
AAADQGFSCYVHPEIEFFLVKELETEGYPPVPTDNGGYFDQAVTDDAPAFRAHAISALEH
HHHCCCCEEEECCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
MGISVEFSHHENAPGQQEIDLRYADALTMADNVMTFRYLIKQVARRNGVAATFMPKPFHE
CCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHH
HAGSAMHTHMSLFEGENNAFHDPDDELRLSGTAKSFIAGILEHAPEITAVTNQWVNSYKR
HCCCHHHHHHHHHCCCCCCCCCCCCCEEECCHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
ITSGDEAPRAAAWGASNRSAMVRLPMYTQNKAASRRVEVRSPDSACNPYLAYSVLLAAGL
HCCCCCCCCHHHCCCCCCCEEEEECCCCCCHHHHCEEEECCCCCCCCHHHHHHHHHHHHH
KGIEEGYELPNPAEEDVSRMTRRERIAAGYKDLPSDLNSALREMERSELVADALGEHVFE
HHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FFLRNKWREWYEYQSQITPWELRNNLSF
HHHHHHHHHHHHHHHCCCHHHHHCCCCC
>Mature Secondary Structure 
GDMNRQQEYVLRTLEERDIRFVRLWFTDIQGYLKSVAVVPAELEGAFQEGIGFDGSAIE
CCCCHHHHHHHHHHHHCCCEEEEHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCHHHH
GFSRIAESDTIAQPDPSTFQIFPFKSDDGALSARMFCDIAMPDGTPSWADPRQVLRRQMK
HHHHHHCCCCCCCCCCCCEEEEEEECCCCCEEEEEEEEEECCCCCCCCCCHHHHHHHHHH
AAADQGFSCYVHPEIEFFLVKELETEGYPPVPTDNGGYFDQAVTDDAPAFRAHAISALEH
HHHCCCCEEEECCCEEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
MGISVEFSHHENAPGQQEIDLRYADALTMADNVMTFRYLIKQVARRNGVAATFMPKPFHE
CCCEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCHHH
HAGSAMHTHMSLFEGENNAFHDPDDELRLSGTAKSFIAGILEHAPEITAVTNQWVNSYKR
HCCCHHHHHHHHHCCCCCCCCCCCCCEEECCHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
ITSGDEAPRAAAWGASNRSAMVRLPMYTQNKAASRRVEVRSPDSACNPYLAYSVLLAAGL
HCCCCCCCCHHHCCCCCCCEEEEECCCCCCHHHHCEEEECCCCCCCCHHHHHHHHHHHHH
KGIEEGYELPNPAEEDVSRMTRRERIAAGYKDLPSDLNSALREMERSELVADALGEHVFE
HHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHH
FFLRNKWREWYEYQSQITPWELRNNLSF
HHHHHHHHHHHHHHHCCCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA