Click here to switch to the map view.

The map label for this gene is glyS [H]

Identifier: 68535685

GI number: 68535685

Start: 739220

End: 740599

Strand: Direct

Name: glyS [H]

Synonym: jk0613

Alternate gene names: 68535685

Gene position: 739220-740599 (Clockwise)

Preceding gene: 68535683

Following gene: 68535686

Centisome position: 30.02

GC content: 61.16

Gene sequence:

>1380_bases
ATGGCAAGCACCATTGATTCCGTCGTCAACCTAGCCAAGCGCAGGGGGCTGGTGTATCCCTGCGGCGAGATCTACGGTGG
CACCCGCTCTGCCTGGGATTACGGCCCGCTGGGTGTGGAGTTGAAGGAGAACATCAAGCGCCAGTGGTGGCGCCACATGG
TTACTTCCCGCCGCGATGTTGTGGGCCTGGATTCTTCCGTGATTCTGCCGAAGCGCGTGTGGGAGGTCTCCGGCCACGTT
GAGGTGTTCACTGACCCGCTGGTTGAGTCCCTGCACACCCACAAGCGTTACCGCGCGGACCACCTGCTGGAGGCTTACGA
GGAGAAGCACGGCCACCCGCCGGCAAATGGTCTGGCCGACATCAACGATCCGGAGACCGGCCAGCCCGGCGCTTGGACCG
AGCCGAAGGCTTTCTCTGGTCTGCTGAAGACCTTCCTGGGGCCCGTTGACGATAAGGAAGGCCTGCACTACCTGCGCCCG
GAGACTGCGCAGGGTATCTTCACGAACTTCAAGAACGTGATGACCACCTCCCGTCAGAAGCCGCCGTTCGGTATCGCGCA
GGTTGGTAAGTCTTTCCGTAACGAGATCACCCCGGGCAACTTCATCTTCCGCACCCGCGAGTTCGAGCAGATGGAGATGG
AGTTCTTCGTCAAGCCAGGCGAGGACGAAGAGTGGCACCAGTACTGGATCGACGATCGCCACCAGTGGTACATCGACCTG
GGCATTAACCCCGATAACCTGCGTCTTTACGAACACCCGAAGGAGAAGCTGTCCCACTACTCCAAGCGCACCGTGGACGT
GGAGTACGCCTTCCACTTCAACGGCTCCAAGTGGGGCGAGCTCGAGGGTGTGGCTAACCGCACCGACTACGACCTGCGCG
TTCACTCTGAGGGTTCGGGCGAGGACTTGAGCTTCTACGACCAGACCACGGAGGAGCGTTGGATCCCTTACACCATCGAG
CCCGCCGCCGGCCTTGGCCGTGCGATGATGGCCTTCCTTGTTGACGCCTACACGGAAGAGGAAGTACCGAATGCGAAGGG
TGGCACGGATACCCGCACGGTGCTGAAGCTGGACCGTCGCCTAGCTCCCGTCAAGATCGCCGTTCTGCCTCTGTCCAAGA
AGGAATCGCTGACTCCGACCGCGGAGAAGATCGCCGGCCAGCTGCGTGGCTTCTGGAACGTGGATTACGACACCTCCGGC
GCTATCGGTCGCCGCTACCGTCGTCAGGATGAGATCGGCACGCCTTTCTGCGTGACTGTCGACTTCGACACTCTCGAGGA
CAATGCCGTGACCGTCCGCGAGCGCGACACCATGGAGCAGGAGCGCATCAAGATCGAGGATCTGCAGAGCTACTTCGCTG
AACGACTGGCGGGCTGCTAA

Upstream 100 bases:

>100_bases
TGGCTTTCACCTTGAACGGGTTAGAATGTTGGGCACAATAGCTGGAAAGCGAAACCTGCATTTCTTGCACGAACACCTCG
ACCAAGGAGACACGCCAACC

Downstream 100 bases:

>100_bases
GCATGTCGGCTGACATTCGCTGGGGCCTGCCGAGTGACGGGCACACGTTCCCCATCTACGAAGGTCCCAGCAATGACCTC
GTGAAGGTGGCGGAGTTTGC

Product: glycyl-tRNA synthetase

Products: NA

Alternate protein names: Glycine--tRNA ligase; GlyRS [H]

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MASTIDSVVNLAKRRGLVYPCGEIYGGTRSAWDYGPLGVELKENIKRQWWRHMVTSRRDVVGLDSSVILPKRVWEVSGHV
EVFTDPLVESLHTHKRYRADHLLEAYEEKHGHPPANGLADINDPETGQPGAWTEPKAFSGLLKTFLGPVDDKEGLHYLRP
ETAQGIFTNFKNVMTTSRQKPPFGIAQVGKSFRNEITPGNFIFRTREFEQMEMEFFVKPGEDEEWHQYWIDDRHQWYIDL
GINPDNLRLYEHPKEKLSHYSKRTVDVEYAFHFNGSKWGELEGVANRTDYDLRVHSEGSGEDLSFYDQTTEERWIPYTIE
PAAGLGRAMMAFLVDAYTEEEVPNAKGGTDTRTVLKLDRRLAPVKIAVLPLSKKESLTPTAEKIAGQLRGFWNVDYDTSG
AIGRRYRRQDEIGTPFCVTVDFDTLEDNAVTVRERDTMEQERIKIEDLQSYFAERLAGC

Sequences:

>Translated_459_residues
MASTIDSVVNLAKRRGLVYPCGEIYGGTRSAWDYGPLGVELKENIKRQWWRHMVTSRRDVVGLDSSVILPKRVWEVSGHV
EVFTDPLVESLHTHKRYRADHLLEAYEEKHGHPPANGLADINDPETGQPGAWTEPKAFSGLLKTFLGPVDDKEGLHYLRP
ETAQGIFTNFKNVMTTSRQKPPFGIAQVGKSFRNEITPGNFIFRTREFEQMEMEFFVKPGEDEEWHQYWIDDRHQWYIDL
GINPDNLRLYEHPKEKLSHYSKRTVDVEYAFHFNGSKWGELEGVANRTDYDLRVHSEGSGEDLSFYDQTTEERWIPYTIE
PAAGLGRAMMAFLVDAYTEEEVPNAKGGTDTRTVLKLDRRLAPVKIAVLPLSKKESLTPTAEKIAGQLRGFWNVDYDTSG
AIGRRYRRQDEIGTPFCVTVDFDTLEDNAVTVRERDTMEQERIKIEDLQSYFAERLAGC
>Mature_458_residues
ASTIDSVVNLAKRRGLVYPCGEIYGGTRSAWDYGPLGVELKENIKRQWWRHMVTSRRDVVGLDSSVILPKRVWEVSGHVE
VFTDPLVESLHTHKRYRADHLLEAYEEKHGHPPANGLADINDPETGQPGAWTEPKAFSGLLKTFLGPVDDKEGLHYLRPE
TAQGIFTNFKNVMTTSRQKPPFGIAQVGKSFRNEITPGNFIFRTREFEQMEMEFFVKPGEDEEWHQYWIDDRHQWYIDLG
INPDNLRLYEHPKEKLSHYSKRTVDVEYAFHFNGSKWGELEGVANRTDYDLRVHSEGSGEDLSFYDQTTEERWIPYTIEP
AAGLGRAMMAFLVDAYTEEEVPNAKGGTDTRTVLKLDRRLAPVKIAVLPLSKKESLTPTAEKIAGQLRGFWNVDYDTSGA
IGRRYRRQDEIGTPFCVTVDFDTLEDNAVTVRERDTMEQERIKIEDLQSYFAERLAGC

Specific function: Catalyzes the attachment of glycine to tRNA(Gly) [H]

COG id: COG0423

COG function: function code J; Glycyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI116805340, Length=584, Percent_Identity=28.5958904109589, Blast_Score=221, Evalue=1e-57,
Organism=Homo sapiens, GI70887790, Length=435, Percent_Identity=22.0689655172414, Blast_Score=94, Evalue=2e-19,
Organism=Caenorhabditis elegans, GI17554996, Length=402, Percent_Identity=27.8606965174129, Blast_Score=157, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI32564541, Length=402, Percent_Identity=27.8606965174129, Blast_Score=156, Evalue=2e-38,
Organism=Saccharomyces cerevisiae, GI6325338, Length=597, Percent_Identity=26.8006700167504, Blast_Score=181, Evalue=2e-46,
Organism=Saccharomyces cerevisiae, GI6319597, Length=398, Percent_Identity=27.3869346733668, Blast_Score=131, Evalue=2e-31,
Organism=Drosophila melanogaster, GI21357965, Length=360, Percent_Identity=31.1111111111111, Blast_Score=163, Evalue=2e-40,
Organism=Drosophila melanogaster, GI24664462, Length=363, Percent_Identity=31.129476584022, Blast_Score=163, Evalue=2e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR022961
- InterPro:   IPR002315 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.14 [H]

Molecular weight: Translated: 52674; Mature: 52542

Theoretical pI: Translated: 5.40; Mature: 5.40

Prosite motif: PS50862 AA_TRNA_LIGASE_II ; PS00307 LECTIN_LEGUME_BETA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MASTIDSVVNLAKRRGLVYPCGEIYGGTRSAWDYGPLGVELKENIKRQWWRHMVTSRRDV
CCCHHHHHHHHHHHCCCEEECHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCE
VGLDSSVILPKRVWEVSGHVEVFTDPLVESLHTHKRYRADHLLEAYEEKHGHPPANGLAD
ECCCCCEECHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
INDPETGQPGAWTEPKAFSGLLKTFLGPVDDKEGLHYLRPETAQGIFTNFKNVMTTSRQK
CCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEECCCCHHHHHHHHHHHHHHCCCCC
PPFGIAQVGKSFRNEITPGNFIFRTREFEQMEMEFFVKPGEDEEWHQYWIDDRHQWYIDL
CCCCHHHHHHHHHHCCCCCCEEEECCHHHHCCEEEEECCCCCCHHHHHCCCCCEEEEEEE
GINPDNLRLYEHPKEKLSHYSKRTVDVEYAFHFNGSKWGELEGVANRTDYDLRVHSEGSG
CCCCCCCEEEECHHHHHHHHHHCEEEEEEEEEECCCCCCCCCCCCCCCCCEEEEECCCCC
EDLSFYDQTTEERWIPYTIEPAAGLGRAMMAFLVDAYTEEEVPNAKGGTDTRTVLKLDRR
CCCCHHHCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHC
LAPVKIAVLPLSKKESLTPTAEKIAGQLRGFWNVDYDTSGAIGRRYRRQDEIGTPFCVTV
CCCEEEEEEECCCCCCCCCHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHCCCCCEEEEE
DFDTLEDNAVTVRERDTMEQERIKIEDLQSYFAERLAGC
ECCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ASTIDSVVNLAKRRGLVYPCGEIYGGTRSAWDYGPLGVELKENIKRQWWRHMVTSRRDV
CCHHHHHHHHHHHCCCEEECHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCE
VGLDSSVILPKRVWEVSGHVEVFTDPLVESLHTHKRYRADHLLEAYEEKHGHPPANGLAD
ECCCCCEECHHHHHHCCCCEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
INDPETGQPGAWTEPKAFSGLLKTFLGPVDDKEGLHYLRPETAQGIFTNFKNVMTTSRQK
CCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCEECCCCHHHHHHHHHHHHHHCCCCC
PPFGIAQVGKSFRNEITPGNFIFRTREFEQMEMEFFVKPGEDEEWHQYWIDDRHQWYIDL
CCCCHHHHHHHHHHCCCCCCEEEECCHHHHCCEEEEECCCCCCHHHHHCCCCCEEEEEEE
GINPDNLRLYEHPKEKLSHYSKRTVDVEYAFHFNGSKWGELEGVANRTDYDLRVHSEGSG
CCCCCCCEEEECHHHHHHHHHHCEEEEEEEEEECCCCCCCCCCCCCCCCCEEEEECCCCC
EDLSFYDQTTEERWIPYTIEPAAGLGRAMMAFLVDAYTEEEVPNAKGGTDTRTVLKLDRR
CCCCHHHCCCCCCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHC
LAPVKIAVLPLSKKESLTPTAEKIAGQLRGFWNVDYDTSGAIGRRYRRQDEIGTPFCVTV
CCCEEEEEEECCCCCCCCCHHHHHHHHHCCCEECCCCCCCHHHHHHHHHHCCCCCEEEEE
DFDTLEDNAVTVRERDTMEQERIKIEDLQSYFAERLAGC
ECCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12840036 [H]