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The map label for this gene is phaD [H]

Identifier: 66766798

GI number: 66766798

Start: 542951

End: 544510

Strand: Reverse

Name: phaD [H]

Synonym: XC_0458

Alternate gene names: 66766798

Gene position: 544510-542951 (Counterclockwise)

Preceding gene: 66766799

Following gene: 66766797

Centisome position: 10.58

GC content: 67.24

Gene sequence:

>1560_bases
ATGAACCATCTGCTGATCCTGCCGATCCTGATCCCGATGCTCGGCGCGTCGGCGTCGTTGTTTGTCGAACACCGCCGCTA
TGGGCCGCGTGTGCAACGCGCCGTGGCCTGGACCAGCATGGCCCTGCTGGCGGTGGCAGTGGTCGCCCTGTTCGCTCGCG
CTGCGCACGGCGAGGTACTGGTGTACCTGCTGGGCGACTGGCCGGCGCGGCTGGGCATCGTGCTGATGGGCGACCGGCTG
GCCGCGTGGATGCTGATGACCACGCTGGTGCTGGGCGCGGCCTGCCTGCTGCACGCCTGCGCCGGCTGGGACCGCCGCGC
GCCACATTTCCATGCGCTGTTCCAGTTCCAGCTGATGGGCTTGAACGGGGCATTTCTGACCGGCGACATCTTCAATCTGT
TCGTGTTCTTCGAGGTGATGCTGATCGCCTCCTACGGCCTGCTGCTCAGCGGCGGGCGCGGGTTGCAGATGCGTATCGGC
CTGCACTACGTGGTGTTCAACGTCTGCGCCTCGACGCTGTTCCTGATTGCGCTGGGGCTGTTGTTCGGTTTGTTCGGCAC
GCTCAACATGGCCGAGCTGTCGCAGCGCATCGCCACCCTGCCCGCAGCGGACGTGCCGCTGGCGAAGGCCACCCTGGGAT
TGTTGTTGCTGGTGTTCTGCAGCAAGGCCGCACTGCTGCCGCTGTACCTGTGGCTGCCGGAAACCTATTCGCGCGCACCG
GCCTCGGTGGCGGCCTTGTTCGCCATCATGACCAAGGTGGGCCTGTACGCGGTGCTGCGGGTGTCGTCGTTGTGGTTCGG
TGCCGGCGCAGGCGCATTGCACGGCTTCGGCCGGCACGCGCTGTTGTGGCTGGGCATCGGCACGCTGGTGATGGCGGCCT
TCGGCGTGATGGCGGCATCGCGGCTGCGGGTGATGGTGTCGTACCTGGTGGTGTTTTCGGCGGCGACGTTGTTCATCGCC
TTCTCGCTGGACGATGCACGTGCGCTGGGCGCCGGCCTGTATTACCTGCCGCATAGCTGCTTTGTGGCGGCGGCGTTGTT
CATGGTGTCGGACCTGATCCGCCGGCGCCGTGGTCGCGCCAGCGATCGCAAGGAAGTGGTTGCCCCACTGCCTGGGCGGG
CGATTCCCGGCGCGATGTTCATGATTGCGGCTGTTGCCGTGGCTGGCCTGCCACCGCTGTCCGGGTTCCTGGCCAAGGCC
GCGCTACTGCAGCATGTGCCCGCCGACCTGGTGGCCCCGGTATGGGGTGCGGTGCTGGGCAGCAGTTTGCTGGTGGTGAT
CGGCCTCACGCGTGCAGGTGTGCGCCTGTTCTGGCGCGTTCCAGAGGCGGTGGAAGAGGCGGTCGAGGACGACGCACCGG
AGCGCCTGCCGCAACGGCGCGCGCGCATGCGCCCGGTGGAAACAGCCGCCACGATCATCCTGTTGAGCGGCTTGGTGGCG
ATGACGATCGGTGCCGGCCCGTTGATGCATTACACCGATGCGGCCGGTGCGCAGCTGCGTGACCCCGGCGCGTATTTGGA
ACAGGTGCGCGGGACCACGCCGCAACGGAGGCAGCCATGA

Upstream 100 bases:

>100_bases
GCCTGGTGCTGGCGATCCGCAGCCGCAGCGACAACGGCAGCGACCACGTGGATGCGCACGAAGATGCGGATACCGGCGAA
GACACGCGTGAGGCGCGCCG

Downstream 100 bases:

>100_bases
GCGCACAGGTGTCCTGGCAACGCCGGCTGTTTCCCTCGCGCCCGTTGAGCGTGATGGTGTTTCTGTTCTGGCTGCTGCTC
AGCGATAGCTTCGGGCCGCA

Product: putative monovalent cation/H+ antiporter subunit D

Products: NAD+; ubiquinol [C]

Alternate protein names: pH adaptation potassium efflux system protein D; Pha system subunit D [H]

Number of amino acids: Translated: 519; Mature: 519

Protein sequence:

>519_residues
MNHLLILPILIPMLGASASLFVEHRRYGPRVQRAVAWTSMALLAVAVVALFARAAHGEVLVYLLGDWPARLGIVLMGDRL
AAWMLMTTLVLGAACLLHACAGWDRRAPHFHALFQFQLMGLNGAFLTGDIFNLFVFFEVMLIASYGLLLSGGRGLQMRIG
LHYVVFNVCASTLFLIALGLLFGLFGTLNMAELSQRIATLPAADVPLAKATLGLLLLVFCSKAALLPLYLWLPETYSRAP
ASVAALFAIMTKVGLYAVLRVSSLWFGAGAGALHGFGRHALLWLGIGTLVMAAFGVMAASRLRVMVSYLVVFSAATLFIA
FSLDDARALGAGLYYLPHSCFVAAALFMVSDLIRRRRGRASDRKEVVAPLPGRAIPGAMFMIAAVAVAGLPPLSGFLAKA
ALLQHVPADLVAPVWGAVLGSSLLVVIGLTRAGVRLFWRVPEAVEEAVEDDAPERLPQRRARMRPVETAATIILLSGLVA
MTIGAGPLMHYTDAAGAQLRDPGAYLEQVRGTTPQRRQP

Sequences:

>Translated_519_residues
MNHLLILPILIPMLGASASLFVEHRRYGPRVQRAVAWTSMALLAVAVVALFARAAHGEVLVYLLGDWPARLGIVLMGDRL
AAWMLMTTLVLGAACLLHACAGWDRRAPHFHALFQFQLMGLNGAFLTGDIFNLFVFFEVMLIASYGLLLSGGRGLQMRIG
LHYVVFNVCASTLFLIALGLLFGLFGTLNMAELSQRIATLPAADVPLAKATLGLLLLVFCSKAALLPLYLWLPETYSRAP
ASVAALFAIMTKVGLYAVLRVSSLWFGAGAGALHGFGRHALLWLGIGTLVMAAFGVMAASRLRVMVSYLVVFSAATLFIA
FSLDDARALGAGLYYLPHSCFVAAALFMVSDLIRRRRGRASDRKEVVAPLPGRAIPGAMFMIAAVAVAGLPPLSGFLAKA
ALLQHVPADLVAPVWGAVLGSSLLVVIGLTRAGVRLFWRVPEAVEEAVEDDAPERLPQRRARMRPVETAATIILLSGLVA
MTIGAGPLMHYTDAAGAQLRDPGAYLEQVRGTTPQRRQP
>Mature_519_residues
MNHLLILPILIPMLGASASLFVEHRRYGPRVQRAVAWTSMALLAVAVVALFARAAHGEVLVYLLGDWPARLGIVLMGDRL
AAWMLMTTLVLGAACLLHACAGWDRRAPHFHALFQFQLMGLNGAFLTGDIFNLFVFFEVMLIASYGLLLSGGRGLQMRIG
LHYVVFNVCASTLFLIALGLLFGLFGTLNMAELSQRIATLPAADVPLAKATLGLLLLVFCSKAALLPLYLWLPETYSRAP
ASVAALFAIMTKVGLYAVLRVSSLWFGAGAGALHGFGRHALLWLGIGTLVMAAFGVMAASRLRVMVSYLVVFSAATLFIA
FSLDDARALGAGLYYLPHSCFVAAALFMVSDLIRRRRGRASDRKEVVAPLPGRAIPGAMFMIAAVAVAGLPPLSGFLAKA
ALLQHVPADLVAPVWGAVLGSSLLVVIGLTRAGVRLFWRVPEAVEEAVEDDAPERLPQRRARMRPVETAATIILLSGLVA
MTIGAGPLMHYTDAAGAQLRDPGAYLEQVRGTTPQRRQP

Specific function: Part of a K(+) efflux system which is required for the adaptation of R.meliloti to alkaline pH as well as for the infection process during symbiotic nodule development [H]

COG id: COG0651

COG function: function code CP; Formate hydrogenlyase subunit 3/Multisubunit Na+/H+ antiporter, MnhD subunit

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CPA3 antiporters (TC 2.A.63) subunit D family [H]

Homologues:

Organism=Escherichia coli, GI1788613, Length=450, Percent_Identity=25.1111111111111, Blast_Score=69, Evalue=6e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004775
- InterPro:   IPR001750 [H]

Pfam domain/function: PF00361 Oxidored_q1 [H]

EC number: 1.6.99.5 [C]

Molecular weight: Translated: 55671; Mature: 55671

Theoretical pI: Translated: 10.33; Mature: 10.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNHLLILPILIPMLGASASLFVEHRRYGPRVQRAVAWTSMALLAVAVVALFARAAHGEVL
CCCEEEHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
VYLLGDWPARLGIVLMGDRLAAWMLMTTLVLGAACLLHACAGWDRRAPHFHALFQFQLMG
EEEECCCCHHHCEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHEEC
LNGAFLTGDIFNLFVFFEVMLIASYGLLLSGGRGLQMRIGLHYVVFNVCASTLFLIALGL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHH
LFGLFGTLNMAELSQRIATLPAADVPLAKATLGLLLLVFCSKAALLPLYLWLPETYSRAP
HHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCH
ASVAALFAIMTKVGLYAVLRVSSLWFGAGAGALHGFGRHALLWLGIGTLVMAAFGVMAAS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
RLRVMVSYLVVFSAATLFIAFSLDDARALGAGLYYLPHSCFVAAALFMVSDLIRRRRGRA
HHHHHHHHHHHHHHHHHHHEECCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SDRKEVVAPLPGRAIPGAMFMIAAVAVAGLPPLSGFLAKAALLQHVPADLVAPVWGAVLG
CCCHHHCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHH
SSLLVVIGLTRAGVRLFWRVPEAVEEAVEDDAPERLPQRRARMRPVETAATIILLSGLVA
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHH
MTIGAGPLMHYTDAAGAQLRDPGAYLEQVRGTTPQRRQP
HHHCCCCHHHCCCCCCCCCCCCHHHHHHHCCCCCCCCCC
>Mature Secondary Structure
MNHLLILPILIPMLGASASLFVEHRRYGPRVQRAVAWTSMALLAVAVVALFARAAHGEVL
CCCEEEHHHHHHHHCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEE
VYLLGDWPARLGIVLMGDRLAAWMLMTTLVLGAACLLHACAGWDRRAPHFHALFQFQLMG
EEEECCCCHHHCEEEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHEEC
LNGAFLTGDIFNLFVFFEVMLIASYGLLLSGGRGLQMRIGLHYVVFNVCASTLFLIALGL
CCCCCHHHHHHHHHHHHHHHHHHHHHHHEECCCCEEEEHHHHHHHHHHHHHHHHHHHHHH
LFGLFGTLNMAELSQRIATLPAADVPLAKATLGLLLLVFCSKAALLPLYLWLPETYSRAP
HHHHHHCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCH
ASVAALFAIMTKVGLYAVLRVSSLWFGAGAGALHGFGRHALLWLGIGTLVMAAFGVMAAS
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHH
RLRVMVSYLVVFSAATLFIAFSLDDARALGAGLYYLPHSCFVAAALFMVSDLIRRRRGRA
HHHHHHHHHHHHHHHHHHHEECCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCC
SDRKEVVAPLPGRAIPGAMFMIAAVAVAGLPPLSGFLAKAALLQHVPADLVAPVWGAVLG
CCCHHHCCCCCCCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHH
SSLLVVIGLTRAGVRLFWRVPEAVEEAVEDDAPERLPQRRARMRPVETAATIILLSGLVA
HHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHH
MTIGAGPLMHYTDAAGAQLRDPGAYLEQVRGTTPQRRQP
HHHCCCCHHHCCCCCCCCCCCCHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: Iron [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADH; ubiquinone [C]

Specific reaction: NADH + ubiquinone = NAD+ + ubiquinol [C]

General reaction: Redox reaction [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9680201; 11481430 [H]