Click here to switch to the map view.

The map label for this gene is mnmG [H]

Identifier: 66766732

GI number: 66766732

Start: 450928

End: 452832

Strand: Reverse

Name: mnmG [H]

Synonym: XC_0389

Alternate gene names: 66766732

Gene position: 452832-450928 (Counterclockwise)

Preceding gene: 66766733

Following gene: 66766731

Centisome position: 8.8

GC content: 67.56

Gene sequence:

>1905_bases
ATGTCCGACTCCTTCTATCGCTACGACGTCATCGTGATTGGTGGCGGCCACGCCGGCACCGAGGCGGCGCTGGCGGCTGC
CCGCGCCGGTGCACGCACGCTGTTGCTGACCCACAACATCGAGACCGTGGGCGCGATGAGCTGCAACCCGGCCATCGGTG
GGATCGGCAAGGGGCACCTGGTCAAGGAGATCGACGCACTGGGCGGGGCGATGGCCAAGGCGGCGGACCTGGCCGGCATC
CAGTGGCGCACCCTCAATGCCTCCAAGGGGCCGGCGGTGCGGGCCACCCGCTGCCAGGCAGACCGCAACCTGTACCGCAG
CGCCATCCGCCGCATCGTCGAAGCGCAGCCCAACCTGACCGTGTTCCAGGCGGCAGTGGACGATCTGATCATCCACAACG
GTGCCGCCGAAGGCGACAGCGTGCGTGGGGTGATCACCCAGACCGGGCTGCGCTTCGAGGCCACGGCCGTGGTCCTGACC
GCAGGCACCTTCCTGGCCGGCAAGATCCATGTCGGCGAGACCCAATACGCGGCCGGGCGCATGGGCGACCCGCCGGCCAC
CACGCTGGCAGCGCGTCTGCGCGAGCGCCCGTTCGCCATCGATCGCCTCAAGACCGGAACTCCGCCGCGTATTGATGGGC
GTACCCTCGACTACACGATGATGGATGAGCAGCCAGGTGATGACCCGCTGCCGGTGATGTCGTTCATGGGCCAGGTCAGC
GACCACCCGACCCAGGTCAGCTGCTGGATCACCCATACCACCGAGCAGACCCACGACATCATCCGTGGCGCGTTGCACCG
CTCGCCGCTGTATTCGGGGCAGATCGAGGGCATTGGCCCGCGCTACTGCCCGTCGATCGAAGACAAGGTGGTGCGTTTCG
CCGACAAGACCAGCCATCAGATCTTTGTCGAACCGGAAGGCCTGGACGTCACCGAGATCTATCCCAACGGTATTTCCACC
TCGCTGCCGTTCGACGTGCAACTGGCGCTGGTGCGCTCGATCCGCGGTTTTGCGCAGGCGCATATCACCCGCCCGGGCTA
CGCGATCGAGTACGACTTCTTCGACCCGCGCGGGCTCAAAGCCTCGCTGGAAACCAAGGCGGTGGGCGGGCTGTTCTTCG
CCGGGCAGATCAACGGCACCACCGGTTACGAAGAAGCCGCCGCGCAGGGCCTGTTGGCCGGGCTCAACGCTGCACGCCAG
GCGCAGGCGCTGCCGGCCTGGTCGCCGCGCCGCGACGAAGCCTACCTGGGCGTACTGGTGGACGACCTGATCACCCACGG
CACCACCGAGCCGTACCGCATGTTCACCAGCCGCGCCGAATACCGGCTGCAGCTGCGCGAAGACAACGCCGATCTGCGGC
TGACCGGCGTGGGCCGCGCCATGGGCCTGGTCGACGATGCGCGCTGGGCACGCTTTTCCAGCAAGCAGGAGGCGGTGCAG
CGCGAAACTGCGCGCCTGTCGGCACTCTGGGCCACGCCGGGCAATGCCCTGGGCCGCGAGGTGGTCGACACCCTGGGCGT
GCCGATGAGCCGCGAGACCAATGTGCTGGACCTGATCAAGCGCCCGGAACTCAGCTACGCCGCGCTGATGCGGGTGCCGA
CGCTGGGCCCGGGCGTGGACGATGCGCAGGTCGCCGAACAAGTGGAAATCGGCGTCAAATACGCCGGTTACCTGAATCGT
CAGCGCGACGAAATCGCCCGCCAGCAGCGCCACGAAACGACTCCGATCCCGGAAGGCTTCGACTACGCCGGCGTGCGTGG
CCTGTCGATGGAAGTGCAGCAGAAACTTGAACGCGTGCGCCCGCAAAGTATCGGCCAGGCACAGCGCATTCCGGGCATGA
CCCCGGCCGCGATCTCGCTGCTGCTGGTGCACCTGGAACGCGCGCGGCGCAGCCAGGTGGCGTGA

Upstream 100 bases:

>100_bases
TGTGACTTTAGTCACATATTTCACGTGTTAATGCCGCAAACCGCCCTTCAGCCGCTACGTTCGCGGCCGCTGGTCTTCCT
CCTGCTTCTGGTCCACGCCC

Downstream 100 bases:

>100_bases
CGCTAGTGGTCGGTGCGCAGGACAGCGGCGGCGTGACGTCACACGCGCCGCCCCGGTCTTGAAGTCTGCATTGGCGTGAG
TGACGGATGTTTCTGGCTGC

Product: tRNA uridine 5-carboxymethylaminomethyl modification enzyme GidA

Products: NA

Alternate protein names: Glucose-inhibited division protein A [H]

Number of amino acids: Translated: 634; Mature: 633

Protein sequence:

>634_residues
MSDSFYRYDVIVIGGGHAGTEAALAAARAGARTLLLTHNIETVGAMSCNPAIGGIGKGHLVKEIDALGGAMAKAADLAGI
QWRTLNASKGPAVRATRCQADRNLYRSAIRRIVEAQPNLTVFQAAVDDLIIHNGAAEGDSVRGVITQTGLRFEATAVVLT
AGTFLAGKIHVGETQYAAGRMGDPPATTLAARLRERPFAIDRLKTGTPPRIDGRTLDYTMMDEQPGDDPLPVMSFMGQVS
DHPTQVSCWITHTTEQTHDIIRGALHRSPLYSGQIEGIGPRYCPSIEDKVVRFADKTSHQIFVEPEGLDVTEIYPNGIST
SLPFDVQLALVRSIRGFAQAHITRPGYAIEYDFFDPRGLKASLETKAVGGLFFAGQINGTTGYEEAAAQGLLAGLNAARQ
AQALPAWSPRRDEAYLGVLVDDLITHGTTEPYRMFTSRAEYRLQLREDNADLRLTGVGRAMGLVDDARWARFSSKQEAVQ
RETARLSALWATPGNALGREVVDTLGVPMSRETNVLDLIKRPELSYAALMRVPTLGPGVDDAQVAEQVEIGVKYAGYLNR
QRDEIARQQRHETTPIPEGFDYAGVRGLSMEVQQKLERVRPQSIGQAQRIPGMTPAAISLLLVHLERARRSQVA

Sequences:

>Translated_634_residues
MSDSFYRYDVIVIGGGHAGTEAALAAARAGARTLLLTHNIETVGAMSCNPAIGGIGKGHLVKEIDALGGAMAKAADLAGI
QWRTLNASKGPAVRATRCQADRNLYRSAIRRIVEAQPNLTVFQAAVDDLIIHNGAAEGDSVRGVITQTGLRFEATAVVLT
AGTFLAGKIHVGETQYAAGRMGDPPATTLAARLRERPFAIDRLKTGTPPRIDGRTLDYTMMDEQPGDDPLPVMSFMGQVS
DHPTQVSCWITHTTEQTHDIIRGALHRSPLYSGQIEGIGPRYCPSIEDKVVRFADKTSHQIFVEPEGLDVTEIYPNGIST
SLPFDVQLALVRSIRGFAQAHITRPGYAIEYDFFDPRGLKASLETKAVGGLFFAGQINGTTGYEEAAAQGLLAGLNAARQ
AQALPAWSPRRDEAYLGVLVDDLITHGTTEPYRMFTSRAEYRLQLREDNADLRLTGVGRAMGLVDDARWARFSSKQEAVQ
RETARLSALWATPGNALGREVVDTLGVPMSRETNVLDLIKRPELSYAALMRVPTLGPGVDDAQVAEQVEIGVKYAGYLNR
QRDEIARQQRHETTPIPEGFDYAGVRGLSMEVQQKLERVRPQSIGQAQRIPGMTPAAISLLLVHLERARRSQVA
>Mature_633_residues
SDSFYRYDVIVIGGGHAGTEAALAAARAGARTLLLTHNIETVGAMSCNPAIGGIGKGHLVKEIDALGGAMAKAADLAGIQ
WRTLNASKGPAVRATRCQADRNLYRSAIRRIVEAQPNLTVFQAAVDDLIIHNGAAEGDSVRGVITQTGLRFEATAVVLTA
GTFLAGKIHVGETQYAAGRMGDPPATTLAARLRERPFAIDRLKTGTPPRIDGRTLDYTMMDEQPGDDPLPVMSFMGQVSD
HPTQVSCWITHTTEQTHDIIRGALHRSPLYSGQIEGIGPRYCPSIEDKVVRFADKTSHQIFVEPEGLDVTEIYPNGISTS
LPFDVQLALVRSIRGFAQAHITRPGYAIEYDFFDPRGLKASLETKAVGGLFFAGQINGTTGYEEAAAQGLLAGLNAARQA
QALPAWSPRRDEAYLGVLVDDLITHGTTEPYRMFTSRAEYRLQLREDNADLRLTGVGRAMGLVDDARWARFSSKQEAVQR
ETARLSALWATPGNALGREVVDTLGVPMSRETNVLDLIKRPELSYAALMRVPTLGPGVDDAQVAEQVEIGVKYAGYLNRQ
RDEIARQQRHETTPIPEGFDYAGVRGLSMEVQQKLERVRPQSIGQAQRIPGMTPAAISLLLVHLERARRSQVA

Specific function: NAD-binding protein involved in the addition of a carboxymethylaminomethyl (cmnm) group at the wobble position (U34) of certain tRNAs, forming tRNA-cmnm(5)s(2)U34 [H]

COG id: COG0445

COG function: function code D; NAD/FAD-utilizing enzyme apparently involved in cell division

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MnmG family [H]

Homologues:

Organism=Homo sapiens, GI74024895, Length=649, Percent_Identity=44.8382126348228, Blast_Score=490, Evalue=1e-138,
Organism=Homo sapiens, GI19882217, Length=674, Percent_Identity=43.1750741839763, Blast_Score=476, Evalue=1e-134,
Organism=Homo sapiens, GI183227703, Length=689, Percent_Identity=42.2351233671988, Blast_Score=471, Evalue=1e-132,
Organism=Escherichia coli, GI2367273, Length=620, Percent_Identity=65.6451612903226, Blast_Score=830, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17534255, Length=624, Percent_Identity=40.224358974359, Blast_Score=454, Evalue=1e-128,
Organism=Saccharomyces cerevisiae, GI6321202, Length=637, Percent_Identity=44.2700156985871, Blast_Score=511, Evalue=1e-146,
Organism=Drosophila melanogaster, GI24658174, Length=640, Percent_Identity=44.0625, Blast_Score=488, Evalue=1e-138,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004416
- InterPro:   IPR002218
- InterPro:   IPR020595 [H]

Pfam domain/function: PF01134 GIDA [H]

EC number: NA

Molecular weight: Translated: 68880; Mature: 68749

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: PS01280 GIDA_1 ; PS01281 GIDA_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDSFYRYDVIVIGGGHAGTEAALAAARAGARTLLLTHNIETVGAMSCNPAIGGIGKGHL
CCCCEEEEEEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCEECCCCCCCCCCCCCCCHH
VKEIDALGGAMAKAADLAGIQWRTLNASKGPAVRATRCQADRNLYRSAIRRIVEAQPNLT
HHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCE
VFQAAVDDLIIHNGAAEGDSVRGVITQTGLRFEATAVVLTAGTFLAGKIHVGETQYAAGR
EHHHHHHHHHEECCCCCCCCCEEEHHHCCCEEEEEEEEEEECCEEEEEEEECCCHHCCCC
MGDPPATTLAARLRERPFAIDRLKTGTPPRIDGRTLDYTMMDEQPGDDPLPVMSFMGQVS
CCCCHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHC
DHPTQVSCWITHTTEQTHDIIRGALHRSPLYSGQIEGIGPRYCPSIEDKVVRFADKTSHQ
CCCCEEEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCE
IFVEPEGLDVTEIYPNGISTSLPFDVQLALVRSIRGFAQAHITRPGYAIEYDFFDPRGLK
EEECCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCC
ASLETKAVGGLFFAGQINGTTGYEEAAAQGLLAGLNAARQAQALPAWSPRRDEAYLGVLV
CCCCHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
DDLITHGTTEPYRMFTSRAEYRLQLREDNADLRLTGVGRAMGLVDDARWARFSSKQEAVQ
HHHHHCCCCCHHHHHHCCCEEEEEEECCCCCEEEEECCHHHCCHHHHHHHHHCCHHHHHH
RETARLSALWATPGNALGREVVDTLGVPMSRETNVLDLIKRPELSYAALMRVPTLGPGVD
HHHHHHEEEECCCCHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCHHHHHCCCCCCCCCC
DAQVAEQVEIGVKYAGYLNRQRDEIARQQRHETTPIPEGFDYAGVRGLSMEVQQKLERVR
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCC
PQSIGQAQRIPGMTPAAISLLLVHLERARRSQVA
CHHCCHHHHCCCCCHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
SDSFYRYDVIVIGGGHAGTEAALAAARAGARTLLLTHNIETVGAMSCNPAIGGIGKGHL
CCCEEEEEEEEEECCCCCHHHHHHHHHCCCEEEEEEECCCEECCCCCCCCCCCCCCCHH
VKEIDALGGAMAKAADLAGIQWRTLNASKGPAVRATRCQADRNLYRSAIRRIVEAQPNLT
HHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCE
VFQAAVDDLIIHNGAAEGDSVRGVITQTGLRFEATAVVLTAGTFLAGKIHVGETQYAAGR
EHHHHHHHHHEECCCCCCCCCEEEHHHCCCEEEEEEEEEEECCEEEEEEEECCCHHCCCC
MGDPPATTLAARLRERPFAIDRLKTGTPPRIDGRTLDYTMMDEQPGDDPLPVMSFMGQVS
CCCCHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCEEEEEEECCCCCCCCCHHHHHHHHHC
DHPTQVSCWITHTTEQTHDIIRGALHRSPLYSGQIEGIGPRYCPSIEDKVVRFADKTSHQ
CCCCEEEEEEEECCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCE
IFVEPEGLDVTEIYPNGISTSLPFDVQLALVRSIRGFAQAHITRPGYAIEYDFFDPRGLK
EEECCCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCEEEEECCCCCCCCC
ASLETKAVGGLFFAGQINGTTGYEEAAAQGLLAGLNAARQAQALPAWSPRRDEAYLGVLV
CCCCHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHH
DDLITHGTTEPYRMFTSRAEYRLQLREDNADLRLTGVGRAMGLVDDARWARFSSKQEAVQ
HHHHHCCCCCHHHHHHCCCEEEEEEECCCCCEEEEECCHHHCCHHHHHHHHHCCHHHHHH
RETARLSALWATPGNALGREVVDTLGVPMSRETNVLDLIKRPELSYAALMRVPTLGPGVD
HHHHHHEEEECCCCHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCHHHHHCCCCCCCCCC
DAQVAEQVEIGVKYAGYLNRQRDEIARQQRHETTPIPEGFDYAGVRGLSMEVQQKLERVR
HHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHCC
PQSIGQAQRIPGMTPAAISLLLVHLERARRSQVA
CHHCCHHHHCCCCCHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]