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The map label for this gene is ynfL [H]

Identifier: 66766656

GI number: 66766656

Start: 369780

End: 370667

Strand: Reverse

Name: ynfL [H]

Synonym: XC_0312

Alternate gene names: 66766656

Gene position: 370667-369780 (Counterclockwise)

Preceding gene: 66766658

Following gene: 66766655

Centisome position: 7.2

GC content: 68.58

Gene sequence:

>888_bases
TTGGAACTTCGCCACCTGCGCTATTTCCTGGCCGTTGCCGAAGAAGGCAACTTCACCCGCGGCGCCGCACGCGTGGGCAT
CGGGCAACCGCCGTTGAGCCAGCAGATCCAGGCACTGGAACGCGACCTGGGCACGCCGTTGTTCCGTCGCACCCATGCCG
GCGCCGAGCTCACCGCGGCCGGCGAGGCGTTCCTGGGCGAGGTGCGCCGGGTGTTGGTCGATGTGGAACGGGCTGCCGAA
ACCGCACGCCGCGTGGCGCGTGGCGAATCCGGCCGGCTGCGGCTGGGCTTCACCGCCTCGGCCGCGTTCAACCCGGTGGT
GCCACGGCTGATCCGCGATTTCCGCCGGCAGTGGCCACAGGTGGAGCTCCTGCTGGAAGAAACCAACACCGCCGGCTTGC
TGGTGGCCCTGGCCGAAGGAAAGCTGGATGCGGCGTTCGTGCGTTACGGCCTGAGCACGCCGCCGGACCTGCAGTTGCTG
CGCTTTGCCGACGAACCGATGAAGATTGCCGTACCCGCCGCGCACCGGCTTGCCGCGTTGGACAGTGCACCGTTGGCGGC
CCTGGCCGGCGAGCCGTTCATCCTGTTTCCGCGCAGCTTCGGCTCCAGCCTGTACGACGAGATCCTGGCGGCCTGCCGCG
CCTCCGGCGTGACCTTGCGCATCACCCAGGAAGCGCCGCAGATGTCATCGATCGTCAACCTGGTGGCCGCCGAATTGGGC
GTCTCGGTGGTGCCCGCCTCCACGGCGCAGCTGCAGCTGCCCGGCGTGCGCTATCTGGACATCGAAGGCCAGATGCCGCT
GGCCCGCCTGGCCTTGGCGGTCTCGCCCGGAGCGCTGGAGAACGCGCCATTGGTGCGGCATTTGTGGACGTTGGCGGAGT
TGATTTGA

Upstream 100 bases:

>100_bases
CACAGGAGCGCAACCGAGACGTGGGACGGCCAGAATGTCCCCCGGCCCACCCCCTGTCCAATATATGATTGTCGCAGCGT
CCATATGCGAAACAGATACC

Downstream 100 bases:

>100_bases
GAGTAGGTGAGTGGCTGCAGACGGTTGCAGTGTCATTGACCAGGTAGGAGCGCGCTTGCGCGCGAGGTGGCTTTACCGGT
AACGCGTTCGCGCACGAGTG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 295; Mature: 295

Protein sequence:

>295_residues
MELRHLRYFLAVAEEGNFTRGAARVGIGQPPLSQQIQALERDLGTPLFRRTHAGAELTAAGEAFLGEVRRVLVDVERAAE
TARRVARGESGRLRLGFTASAAFNPVVPRLIRDFRRQWPQVELLLEETNTAGLLVALAEGKLDAAFVRYGLSTPPDLQLL
RFADEPMKIAVPAAHRLAALDSAPLAALAGEPFILFPRSFGSSLYDEILAACRASGVTLRITQEAPQMSSIVNLVAAELG
VSVVPASTAQLQLPGVRYLDIEGQMPLARLALAVSPGALENAPLVRHLWTLAELI

Sequences:

>Translated_295_residues
MELRHLRYFLAVAEEGNFTRGAARVGIGQPPLSQQIQALERDLGTPLFRRTHAGAELTAAGEAFLGEVRRVLVDVERAAE
TARRVARGESGRLRLGFTASAAFNPVVPRLIRDFRRQWPQVELLLEETNTAGLLVALAEGKLDAAFVRYGLSTPPDLQLL
RFADEPMKIAVPAAHRLAALDSAPLAALAGEPFILFPRSFGSSLYDEILAACRASGVTLRITQEAPQMSSIVNLVAAELG
VSVVPASTAQLQLPGVRYLDIEGQMPLARLALAVSPGALENAPLVRHLWTLAELI
>Mature_295_residues
MELRHLRYFLAVAEEGNFTRGAARVGIGQPPLSQQIQALERDLGTPLFRRTHAGAELTAAGEAFLGEVRRVLVDVERAAE
TARRVARGESGRLRLGFTASAAFNPVVPRLIRDFRRQWPQVELLLEETNTAGLLVALAEGKLDAAFVRYGLSTPPDLQLL
RFADEPMKIAVPAAHRLAALDSAPLAALAGEPFILFPRSFGSSLYDEILAACRASGVTLRITQEAPQMSSIVNLVAAELG
VSVVPASTAQLQLPGVRYLDIEGQMPLARLALAVSPGALENAPLVRHLWTLAELI

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=288, Percent_Identity=43.4027777777778, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI2367136, Length=265, Percent_Identity=33.2075471698113, Blast_Score=152, Evalue=3e-38,
Organism=Escherichia coli, GI1788887, Length=282, Percent_Identity=32.9787234042553, Blast_Score=130, Evalue=9e-32,
Organism=Escherichia coli, GI1790399, Length=297, Percent_Identity=30.6397306397306, Blast_Score=115, Evalue=3e-27,
Organism=Escherichia coli, GI157672245, Length=246, Percent_Identity=30.4878048780488, Blast_Score=108, Evalue=4e-25,
Organism=Escherichia coli, GI1788748, Length=248, Percent_Identity=26.2096774193548, Blast_Score=87, Evalue=9e-19,
Organism=Escherichia coli, GI1788297, Length=258, Percent_Identity=26.7441860465116, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI87081904, Length=180, Percent_Identity=31.6666666666667, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1787530, Length=246, Percent_Identity=27.2357723577236, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787601, Length=150, Percent_Identity=32.6666666666667, Blast_Score=75, Evalue=4e-15,
Organism=Escherichia coli, GI145693105, Length=300, Percent_Identity=26, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1787949, Length=295, Percent_Identity=26.4406779661017, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87082132, Length=255, Percent_Identity=26.6666666666667, Blast_Score=69, Evalue=5e-13,
Organism=Escherichia coli, GI1790262, Length=256, Percent_Identity=26.171875, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1790208, Length=146, Percent_Identity=34.2465753424658, Blast_Score=68, Evalue=8e-13,
Organism=Escherichia coli, GI1789506, Length=272, Percent_Identity=22.7941176470588, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787806, Length=149, Percent_Identity=33.5570469798658, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787589, Length=169, Percent_Identity=33.1360946745562, Blast_Score=65, Evalue=8e-12,
Organism=Escherichia coli, GI1786401, Length=154, Percent_Identity=29.8701298701299, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 31851; Mature: 31851

Theoretical pI: Translated: 6.97; Mature: 6.97

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MELRHLRYFLAVAEEGNFTRGAARVGIGQPPLSQQIQALERDLGTPLFRRTHAGAELTAA
CCHHHEEEEEEEECCCCCCCCHHCCCCCCCCHHHHHHHHHHHHCCHHHHHHCCCCCEEHH
GEAFLGEVRRVLVDVERAAETARRVARGESGRLRLGFTASAAFNPVVPRLIRDFRRQWPQ
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCC
VELLLEETNTAGLLVALAEGKLDAAFVRYGLSTPPDLQLLRFADEPMKIAVPAAHRLAAL
EEEEEECCCCCEEEEEEECCCHHHHHHHHCCCCCCCHHHEEECCCCCEEECCHHHHHHHH
DSAPLAALAGEPFILFPRSFGSSLYDEILAACRASGVTLRITQEAPQMSSIVNLVAAELG
CCCCHHHHCCCCEEEECHHHCHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHC
VSVVPASTAQLQLPGVRYLDIEGQMPLARLALAVSPGALENAPLVRHLWTLAELI
CEEECCCCCEEECCCEEEEEECCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHC
>Mature Secondary Structure
MELRHLRYFLAVAEEGNFTRGAARVGIGQPPLSQQIQALERDLGTPLFRRTHAGAELTAA
CCHHHEEEEEEEECCCCCCCCHHCCCCCCCCHHHHHHHHHHHHCCHHHHHHCCCCCEEHH
GEAFLGEVRRVLVDVERAAETARRVARGESGRLRLGFTASAAFNPVVPRLIRDFRRQWPQ
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHCCCC
VELLLEETNTAGLLVALAEGKLDAAFVRYGLSTPPDLQLLRFADEPMKIAVPAAHRLAAL
EEEEEECCCCCEEEEEEECCCHHHHHHHHCCCCCCCHHHEEECCCCCEEECCHHHHHHHH
DSAPLAALAGEPFILFPRSFGSSLYDEILAACRASGVTLRITQEAPQMSSIVNLVAAELG
CCCCHHHHCCCCEEEECHHHCHHHHHHHHHHHHCCCCEEEEECCCHHHHHHHHHHHHHHC
VSVVPASTAQLQLPGVRYLDIEGQMPLARLALAVSPGALENAPLVRHLWTLAELI
CEEECCCCCEEECCCEEEEEECCCCCHHHHHHHCCCCCCCCCHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]