Click here to switch to the map view.

The map label for this gene is gltD [H]

Identifier: 66766383

GI number: 66766383

Start: 38001

End: 39482

Strand: Reverse

Name: gltD [H]

Synonym: XC_0031

Alternate gene names: 66766383

Gene position: 39482-38001 (Counterclockwise)

Preceding gene: 66766384

Following gene: 66766382

Centisome position: 0.77

GC content: 68.49

Gene sequence:

>1482_bases
ATGAGCCGCAAGCAAGCCTTCCAATTCCTTGACCTGCCGCGGACCATGCCCACGCGCATCCCGGTGGAGCTGCGTACGTC
CGGTGACTGGGGCGAGTTGTACGGCAAGTTCGACAAGGCCGACGCGCAGTACCAGTCCGGCCGCTGTCTGGATTGCGGCA
ACCCGTACTGCAGCTGGAAGTGCCCGGTGCACAACGCCATTCCGCAGTGGCTGCAGCTGGTGCAGGAAGATCGCATCATC
GAGGCCGCCGCGCTGTGCCATTCCACCAACCCGCTGCCGGAAGTGTGCGGGCGCGTGTGTCCGCAGGACCGTCTGTGCGA
AGGCAGCTGCACGCTGGAAGAATTCGGCGCGGTCACCATCGGCGCGGTGGAAAAGTACATCGTCGATACCGCCTTCAGCA
TGGGCTGGCGCCCGGACCTGGGCAACGTGCAACCCAGCGGCCACCGCGTGGCCGTGGTCGGCGCCGGCCCGGCAGGCCTG
TCCTGCGCGGACCGCCTGGTGCGCGCCGGCATCGAAGCGGTGGTCTACGACCGCTACGAACAGATCGGCGGGCTGCTGCA
GTTCGGCATTCCTAGCTTCAAGCTGGACAAGTCGGTGATCGGCAAGCGCCGCGAGATCCTGGAAGGCATGGGCGTGCAGT
TCCGCCTGGGCGTGGAGATCGGCAAGGACGTCAGCATCGAACAGTTGCTCGGCGAATACGACGCCGTGTTCCTGGGCACC
GGCGCGTACCGCTACACCGACGGCGGGCTGGACGGCCAGGACCTGAAGAACGTTTTGCCAGCGTTGCCCTTCCTGGTGCA
GAACAGCCGCATCGTCAGCGGCAACGACCCGCACGGCCGGCCGATCGCCGGCTGGGAAGACCAGATCGCCCTGCCCGACC
TCAACGGCAAGCGCGTGGTGGTGCTGGGCGGCGGCGACACCGGCATGGACTGCGTGCGCAGCGCCATCCGCCTGGGCGCG
GCCAAAGTCACCTGTGCCTACCGCCGCGACGAATCCAGCATGCCGGGCTCGGCGCGCGAAGTGGCCAACGCGCGCGAAGA
AGGCGTGCGCTTCCTGTTCAACCGCCAGCCGCTGTCGATCGAGTCCGGCGCCGACGACGAAGCCATCGGCGTGACCGTGG
TGGAAACCAAACTCGGCGAGCCCGACGCCAGCGGCCGCCGCAACGCGGTGCCGGTGGAAGGCAGCGAATCGTTGCTGGAA
GCGGACGTGGTGATCATCGCGTTCGGCTTCTCGCCGACGGTGCCGGAGTGGCTGTCCTCGCAAGGCGTGGAGGCCGGCAG
CAACGGTCGCATCGTCGCCCCGGCCGAGGGCAACGGCCGCCTGCCCTACCAGACCAGCAACCCCCGCCTGTTCGCCGGTG
GCGACTGCGTGCGCGGCGCCGACCTGGTGGTCACCGCCGTGGCCGAAGGCCGCGATGCGGCCGGCAGCATCGTGCAGCTG
CTGGGTGTCAAAGCGCAGGTCAAGGAGCCGGCTGCGGCGTGA

Upstream 100 bases:

>100_bases
AGGGACTCGGGAATTGTAAAACTGGTTCCCCGCCCTTCTTCCCGTTCGTCGCGACCCGCTGTTCCGATTCCCCATTCCCC
ATTCCCGATTCCCAGCCCCT

Downstream 100 bases:

>100_bases
GCGTTCGCTAACCAAAGATATGATCGCCCCTACCGTCAAAGGGTATGGTGACTCTGGCGCTGAGTCGTTCGACGATTACT
ACGCTCGATGGCCGAAACAG

Product: glutamate synthase subunit beta

Products: NA

Alternate protein names: Glutamate synthase subunit beta; GLTS beta chain; NADPH-GOGAT [H]

Number of amino acids: Translated: 493; Mature: 492

Protein sequence:

>493_residues
MSRKQAFQFLDLPRTMPTRIPVELRTSGDWGELYGKFDKADAQYQSGRCLDCGNPYCSWKCPVHNAIPQWLQLVQEDRII
EAAALCHSTNPLPEVCGRVCPQDRLCEGSCTLEEFGAVTIGAVEKYIVDTAFSMGWRPDLGNVQPSGHRVAVVGAGPAGL
SCADRLVRAGIEAVVYDRYEQIGGLLQFGIPSFKLDKSVIGKRREILEGMGVQFRLGVEIGKDVSIEQLLGEYDAVFLGT
GAYRYTDGGLDGQDLKNVLPALPFLVQNSRIVSGNDPHGRPIAGWEDQIALPDLNGKRVVVLGGGDTGMDCVRSAIRLGA
AKVTCAYRRDESSMPGSAREVANAREEGVRFLFNRQPLSIESGADDEAIGVTVVETKLGEPDASGRRNAVPVEGSESLLE
ADVVIIAFGFSPTVPEWLSSQGVEAGSNGRIVAPAEGNGRLPYQTSNPRLFAGGDCVRGADLVVTAVAEGRDAAGSIVQL
LGVKAQVKEPAAA

Sequences:

>Translated_493_residues
MSRKQAFQFLDLPRTMPTRIPVELRTSGDWGELYGKFDKADAQYQSGRCLDCGNPYCSWKCPVHNAIPQWLQLVQEDRII
EAAALCHSTNPLPEVCGRVCPQDRLCEGSCTLEEFGAVTIGAVEKYIVDTAFSMGWRPDLGNVQPSGHRVAVVGAGPAGL
SCADRLVRAGIEAVVYDRYEQIGGLLQFGIPSFKLDKSVIGKRREILEGMGVQFRLGVEIGKDVSIEQLLGEYDAVFLGT
GAYRYTDGGLDGQDLKNVLPALPFLVQNSRIVSGNDPHGRPIAGWEDQIALPDLNGKRVVVLGGGDTGMDCVRSAIRLGA
AKVTCAYRRDESSMPGSAREVANAREEGVRFLFNRQPLSIESGADDEAIGVTVVETKLGEPDASGRRNAVPVEGSESLLE
ADVVIIAFGFSPTVPEWLSSQGVEAGSNGRIVAPAEGNGRLPYQTSNPRLFAGGDCVRGADLVVTAVAEGRDAAGSIVQL
LGVKAQVKEPAAA
>Mature_492_residues
SRKQAFQFLDLPRTMPTRIPVELRTSGDWGELYGKFDKADAQYQSGRCLDCGNPYCSWKCPVHNAIPQWLQLVQEDRIIE
AAALCHSTNPLPEVCGRVCPQDRLCEGSCTLEEFGAVTIGAVEKYIVDTAFSMGWRPDLGNVQPSGHRVAVVGAGPAGLS
CADRLVRAGIEAVVYDRYEQIGGLLQFGIPSFKLDKSVIGKRREILEGMGVQFRLGVEIGKDVSIEQLLGEYDAVFLGTG
AYRYTDGGLDGQDLKNVLPALPFLVQNSRIVSGNDPHGRPIAGWEDQIALPDLNGKRVVVLGGGDTGMDCVRSAIRLGAA
KVTCAYRRDESSMPGSAREVANAREEGVRFLFNRQPLSIESGADDEAIGVTVVETKLGEPDASGRRNAVPVEGSESLLEA
DVVIIAFGFSPTVPEWLSSQGVEAGSNGRIVAPAEGNGRLPYQTSNPRLFAGGDCVRGADLVVTAVAEGRDAAGSIVQLL
GVKAQVKEPAAA

Specific function: NITROGEN METABOLISM, GLUTAMATE BIOSYNTHESIS. THE CATALYZED REACTION BRINGS TOGETHER THE NITROGEN AND CARBON METABOLISM. [C]

COG id: COG0493

COG function: function code ER; NADPH-dependent glutamate synthase beta chain and related oxidoreductases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 4Fe-4S ferredoxin-type domain [H]

Homologues:

Organism=Homo sapiens, GI119943098, Length=462, Percent_Identity=29.2207792207792, Blast_Score=146, Evalue=5e-35,
Organism=Escherichia coli, GI1789606, Length=482, Percent_Identity=57.4688796680498, Blast_Score=561, Evalue=1e-161,
Organism=Escherichia coli, GI1788811, Length=476, Percent_Identity=54.4117647058823, Blast_Score=514, Evalue=1e-147,
Organism=Escherichia coli, GI87082180, Length=459, Percent_Identity=53.1590413943355, Blast_Score=477, Evalue=1e-136,
Organism=Escherichia coli, GI1788468, Length=452, Percent_Identity=28.5398230088496, Blast_Score=164, Evalue=2e-41,
Organism=Escherichia coli, GI1789243, Length=443, Percent_Identity=31.1512415349887, Blast_Score=155, Evalue=6e-39,
Organism=Caenorhabditis elegans, GI17570289, Length=491, Percent_Identity=33.8085539714868, Blast_Score=235, Evalue=4e-62,
Organism=Caenorhabditis elegans, GI71984108, Length=462, Percent_Identity=30.5194805194805, Blast_Score=148, Evalue=8e-36,
Organism=Caenorhabditis elegans, GI17543860, Length=172, Percent_Identity=28.4883720930233, Blast_Score=73, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6320030, Length=476, Percent_Identity=34.6638655462185, Blast_Score=250, Evalue=4e-67,
Organism=Drosophila melanogaster, GI28574881, Length=478, Percent_Identity=37.6569037656904, Blast_Score=260, Evalue=1e-69,
Organism=Drosophila melanogaster, GI24665539, Length=478, Percent_Identity=37.6569037656904, Blast_Score=260, Evalue=1e-69,
Organism=Drosophila melanogaster, GI24665547, Length=478, Percent_Identity=37.6569037656904, Blast_Score=260, Evalue=2e-69,
Organism=Drosophila melanogaster, GI24665543, Length=478, Percent_Identity=37.6569037656904, Blast_Score=260, Evalue=2e-69,
Organism=Drosophila melanogaster, GI24640763, Length=454, Percent_Identity=30.6167400881057, Blast_Score=165, Evalue=8e-41,
Organism=Drosophila melanogaster, GI18858217, Length=454, Percent_Identity=30.6167400881057, Blast_Score=165, Evalue=8e-41,

Paralogues:

None

Copy number: 620 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 180 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR013027
- InterPro:   IPR012285
- InterPro:   IPR006006
- InterPro:   IPR009051
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2 [H]

EC number: =1.4.1.13 [H]

Molecular weight: Translated: 52732; Mature: 52601

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
2.6 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRKQAFQFLDLPRTMPTRIPVELRTSGDWGELYGKFDKADAQYQSGRCLDCGNPYCSWK
CCHHHHHHHHCCCCCCCCCCCEEEECCCCHHHHHCCCCCCCCHHCCCCEEECCCCCCEEE
CPVHNAIPQWLQLVQEDRIIEAAALCHSTNPLPEVCGRVCPQDRLCEGSCTLEEFGAVTI
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCHHHHCCEEH
GAVEKYIVDTAFSMGWRPDLGNVQPSGHRVAVVGAGPAGLSCADRLVRAGIEAVVYDRYE
HHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
QIGGLLQFGIPSFKLDKSVIGKRREILEGMGVQFRLGVEIGKDVSIEQLLGEYDAVFLGT
HHCCHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHCCCCEEEEEC
GAYRYTDGGLDGQDLKNVLPALPFLVQNSRIVSGNDPHGRPIAGWEDQIALPDLNGKRVV
CCEEECCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCEECCCCCCCEEE
VLGGGDTGMDCVRSAIRLGAAKVTCAYRRDESSMPGSAREVANAREEGVRFLFNRQPLSI
EECCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHEEECCCCCCC
ESGADDEAIGVTVVETKLGEPDASGRRNAVPVEGSESLLEADVVIIAFGFSPTVPEWLSS
CCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHCCEEEEEECCCCCCHHHHHH
QGVEAGSNGRIVAPAEGNGRLPYQTSNPRLFAGGDCVRGADLVVTAVAEGRDAAGSIVQL
CCCCCCCCCCEEEECCCCCCCCEECCCCEEEECCCCCCCCCEEEEEECCCCCHHHHHHHH
LGVKAQVKEPAAA
HCCHHHCCCCCCC
>Mature Secondary Structure 
SRKQAFQFLDLPRTMPTRIPVELRTSGDWGELYGKFDKADAQYQSGRCLDCGNPYCSWK
CHHHHHHHHCCCCCCCCCCCEEEECCCCHHHHHCCCCCCCCHHCCCCEEECCCCCCEEE
CPVHNAIPQWLQLVQEDRIIEAAALCHSTNPLPEVCGRVCPQDRLCEGSCTLEEFGAVTI
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCCHHHHCCEEH
GAVEKYIVDTAFSMGWRPDLGNVQPSGHRVAVVGAGPAGLSCADRLVRAGIEAVVYDRYE
HHHHHHHHHHHHHCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHH
QIGGLLQFGIPSFKLDKSVIGKRREILEGMGVQFRLGVEIGKDVSIEQLLGEYDAVFLGT
HHCCHHHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEEEECCCCCCHHHHHCCCCEEEEEC
GAYRYTDGGLDGQDLKNVLPALPFLVQNSRIVSGNDPHGRPIAGWEDQIALPDLNGKRVV
CCEEECCCCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCCCCCCCCCCEECCCCCCCEEE
VLGGGDTGMDCVRSAIRLGAAKVTCAYRRDESSMPGSAREVANAREEGVRFLFNRQPLSI
EECCCCCCHHHHHHHHHHCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHEEECCCCCCC
ESGADDEAIGVTVVETKLGEPDASGRRNAVPVEGSESLLEADVVIIAFGFSPTVPEWLSS
CCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCHHHHHCCEEEEEECCCCCCHHHHHH
QGVEAGSNGRIVAPAEGNGRLPYQTSNPRLFAGGDCVRGADLVVTAVAEGRDAAGSIVQL
CCCCCCCCCCEEEECCCCCCCCEECCCCEEEECCCCCCCCCEEEEEECCCCCHHHHHHHH
LGVKAQVKEPAAA
HCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 3326786; 9278503; 9298646; 9600841; 2643092 [H]