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The map label for this gene is cls [H]

Identifier: 66766366

GI number: 66766366

Start: 15235

End: 16695

Strand: Reverse

Name: cls [H]

Synonym: XC_0014

Alternate gene names: 66766366

Gene position: 16695-15235 (Counterclockwise)

Preceding gene: 77761059

Following gene: 66766365

Centisome position: 0.32

GC content: 66.26

Gene sequence:

>1461_bases
ATGCTCGCACTTGCACAGGGCGCGTGGGATTGGCTGGCCGACATTCCCCACCTCAAAGCGCTCCTCTTCGCCGCCTACGT
GCTGTATCTGCTGTGGATCGTCAGCTGGATCATCCTGCAGAAGCGCGAGCCGGTGGCCACGCTGAGCTGGGTGCTGTCGC
TGGCCGCGCTGCCGTATCTGGGCCTGCTGATCTACTACTGGCTGGGCCCGCAAAAGGTCAAACGCCAGCGTCTACGCCGC
GGGCGGTCGCGTTCGGGTATGGAAAGCTACAGCAGCGTGTGTCCGCCCGATGCCGATTGCACCGAGCTGGCCAAGATCGC
CCAATCCACCACCGGCCTGGCCCCAAGCAGCGCGACAGAAGCCTTGTGGCTGGTGGATGGCGCGTCCACCTATGCGGCGA
TCATCGAGGCGATCACACAGGCGCGCGACCACATCCACCTGGAGTACTACATTTTCCAGCCCGACCGCAGCGGCATGCCG
ATCTGCGAGGCCTTGATGGAACGCGCGCGGGCGGGGGTCAAGGTGCGGCTGTTGATGGATGCAGTCGGTTCCTCGGCGAT
GACCCGGCGCACCCTGCGCACGCTGCGTGCGGCCGGCGTGGAGACCGCCTGGTTCCATCCCACCCAGCTGCTCAAGCCGT
TCAAGCGGCCGTGGTTGAACCTGCGTACGCACCGCAAGTTGATCGTCATCGACGGCCGCATCGCGTTCACCGGCGGCATC
AATGTCACCGACGAAGAGAACGAACAGGTTCGCCCCGACGCGTTTCGCGACCTGCATGTGCGTCTGCAGGGCCACGTGGT
GCGCAGCCTGCAGCTGGTGTTCCTGGAGGACTGGCTCTACGCCACCCGCCAGGCCCAGGGCGCCTTCCATGGCCAACAAC
TGTGGCCGGAGGATGTGCCCACCCGCAAGCAGGGCAGCATCGATGCGCAGGTGCTGGTGTCCGGCCCCGATTCGTCCTGG
GAAGCGATCCACCGGCTGATGGTGGCCGCCATCCACGAAGCCAGGCATCGGGTCTGGCTGGTCACGCCGTACTTCGTGCC
GGGCGAGGCCGCGCGCATGGCGCTCACCTCTGCGGCGCTGGGCGGGCTGGACGTGCGCCTGCTGATCCCGCGCGTCAGCG
ATTCGCGCCTGGTCACCTATGCCGCGCGCTCGTATTTCGATGAACTGCTGGAGGCCGGCGTGCGTATCTACGAGTACGGC
CCGCGCATGTTGCACACCAAGGCGCTGATCGCCGACGACGATGTCTGCATCGTCGGTAGCGCCAATTTCGACAGCCGCAG
CTTCCGGCTGAACTTCGAGCTGTCGATGCTGTTCCGCGACCAGGCAGTAGCCGCCGAGCTCGCCGGCATGATCGGCACCG
ACATGGCCCAGGCACAGGAAGTGCGCTTCAACCTGCGCCGGCCGTTCTGGCGATCGCGCCTGCCGGAAGCCTTCGCGCGG
CTGCTATCGCCGCTGCTCTGA

Upstream 100 bases:

>100_bases
GAGCCCTAATCCGCAGCTTTCCCGTGCATGTGCTACCAGGATCGCCAGGGCAGCAGCCGGCAGTCATCGCAGCACAGCGT
GTGCGCCGTAGAATCCGCGC

Downstream 100 bases:

>100_bases
CCGCAGCGCCGCGCCCCACGTGGAACCACGCCGGGCGCAGCGCTACACTGCCGCCACTCAACGGGGAGCTCGTCATGCAC
TGGCTGTTTCTGCTTATGGC

Product: cardiolipin synthetase

Products: Biphosphatidylglycerol; Glycerol. [C]

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 486; Mature: 486

Protein sequence:

>486_residues
MLALAQGAWDWLADIPHLKALLFAAYVLYLLWIVSWIILQKREPVATLSWVLSLAALPYLGLLIYYWLGPQKVKRQRLRR
GRSRSGMESYSSVCPPDADCTELAKIAQSTTGLAPSSATEALWLVDGASTYAAIIEAITQARDHIHLEYYIFQPDRSGMP
ICEALMERARAGVKVRLLMDAVGSSAMTRRTLRTLRAAGVETAWFHPTQLLKPFKRPWLNLRTHRKLIVIDGRIAFTGGI
NVTDEENEQVRPDAFRDLHVRLQGHVVRSLQLVFLEDWLYATRQAQGAFHGQQLWPEDVPTRKQGSIDAQVLVSGPDSSW
EAIHRLMVAAIHEARHRVWLVTPYFVPGEAARMALTSAALGGLDVRLLIPRVSDSRLVTYAARSYFDELLEAGVRIYEYG
PRMLHTKALIADDDVCIVGSANFDSRSFRLNFELSMLFRDQAVAAELAGMIGTDMAQAQEVRFNLRRPFWRSRLPEAFAR
LLSPLL

Sequences:

>Translated_486_residues
MLALAQGAWDWLADIPHLKALLFAAYVLYLLWIVSWIILQKREPVATLSWVLSLAALPYLGLLIYYWLGPQKVKRQRLRR
GRSRSGMESYSSVCPPDADCTELAKIAQSTTGLAPSSATEALWLVDGASTYAAIIEAITQARDHIHLEYYIFQPDRSGMP
ICEALMERARAGVKVRLLMDAVGSSAMTRRTLRTLRAAGVETAWFHPTQLLKPFKRPWLNLRTHRKLIVIDGRIAFTGGI
NVTDEENEQVRPDAFRDLHVRLQGHVVRSLQLVFLEDWLYATRQAQGAFHGQQLWPEDVPTRKQGSIDAQVLVSGPDSSW
EAIHRLMVAAIHEARHRVWLVTPYFVPGEAARMALTSAALGGLDVRLLIPRVSDSRLVTYAARSYFDELLEAGVRIYEYG
PRMLHTKALIADDDVCIVGSANFDSRSFRLNFELSMLFRDQAVAAELAGMIGTDMAQAQEVRFNLRRPFWRSRLPEAFAR
LLSPLL
>Mature_486_residues
MLALAQGAWDWLADIPHLKALLFAAYVLYLLWIVSWIILQKREPVATLSWVLSLAALPYLGLLIYYWLGPQKVKRQRLRR
GRSRSGMESYSSVCPPDADCTELAKIAQSTTGLAPSSATEALWLVDGASTYAAIIEAITQARDHIHLEYYIFQPDRSGMP
ICEALMERARAGVKVRLLMDAVGSSAMTRRTLRTLRAAGVETAWFHPTQLLKPFKRPWLNLRTHRKLIVIDGRIAFTGGI
NVTDEENEQVRPDAFRDLHVRLQGHVVRSLQLVFLEDWLYATRQAQGAFHGQQLWPEDVPTRKQGSIDAQVLVSGPDSSW
EAIHRLMVAAIHEARHRVWLVTPYFVPGEAARMALTSAALGGLDVRLLIPRVSDSRLVTYAARSYFDELLEAGVRIYEYG
PRMLHTKALIADDDVCIVGSANFDSRSFRLNFELSMLFRDQAVAAELAGMIGTDMAQAQEVRFNLRRPFWRSRLPEAFAR
LLSPLL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=476, Percent_Identity=29.8319327731092, Blast_Score=223, Evalue=2e-59,
Organism=Escherichia coli, GI1787007, Length=344, Percent_Identity=33.1395348837209, Blast_Score=148, Evalue=1e-36,
Organism=Escherichia coli, GI87081826, Length=396, Percent_Identity=25.7575757575758, Blast_Score=70, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR015679
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.- [C]

Molecular weight: Translated: 54832; Mature: 54832

Theoretical pI: Translated: 9.35; Mature: 9.35

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLALAQGAWDWLADIPHLKALLFAAYVLYLLWIVSWIILQKREPVATLSWVLSLAALPYL
CCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
GLLIYYWLGPQKVKRQRLRRGRSRSGMESYSSVCPPDADCTELAKIAQSTTGLAPSSATE
HHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCC
ALWLVDGASTYAAIIEAITQARDHIHLEYYIFQPDRSGMPICEALMERARAGVKVRLLMD
EEEEEECCHHHHHHHHHHHHHHHCEEEEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEHH
AVGSSAMTRRTLRTLRAAGVETAWFHPTQLLKPFKRPWLNLRTHRKLIVIDGRIAFTGGI
HHCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEEEECEEEEECCC
NVTDEENEQVRPDAFRDLHVRLQGHVVRSLQLVFLEDWLYATRQAQGAFHGQQLWPEDVP
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
TRKQGSIDAQVLVSGPDSSWEAIHRLMVAAIHEARHRVWLVTPYFVPGEAARMALTSAAL
CCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCEEEEEECEECCCHHHHHHHHHHHH
GGLDVRLLIPRVSDSRLVTYAARSYFDELLEAGVRIYEYGPRMLHTKALIADDDVCIVGS
CCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEEEECCCEEEEEC
ANFDSRSFRLNFELSMLFRDQAVAAELAGMIGTDMAQAQEVRFNLRRPFWRSRLPEAFAR
CCCCCCEEEEEEEEEEEHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHHHHHHHHH
LLSPLL
HHHCCC
>Mature Secondary Structure
MLALAQGAWDWLADIPHLKALLFAAYVLYLLWIVSWIILQKREPVATLSWVLSLAALPYL
CCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
GLLIYYWLGPQKVKRQRLRRGRSRSGMESYSSVCPPDADCTELAKIAQSTTGLAPSSATE
HHHHHHHCCHHHHHHHHHHCCCCCCCHHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCC
ALWLVDGASTYAAIIEAITQARDHIHLEYYIFQPDRSGMPICEALMERARAGVKVRLLMD
EEEEEECCHHHHHHHHHHHHHHHCEEEEEEEECCCCCCCHHHHHHHHHHHCCCEEEEEHH
AVGSSAMTRRTLRTLRAAGVETAWFHPTQLLKPFKRPWLNLRTHRKLIVIDGRIAFTGGI
HHCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCEEEEEECEEEEECCC
NVTDEENEQVRPDAFRDLHVRLQGHVVRSLQLVFLEDWLYATRQAQGAFHGQQLWPEDVP
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCC
TRKQGSIDAQVLVSGPDSSWEAIHRLMVAAIHEARHRVWLVTPYFVPGEAARMALTSAAL
CCCCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCEEEEEECEECCCHHHHHHHHHHHH
GGLDVRLLIPRVSDSRLVTYAARSYFDELLEAGVRIYEYGPRMLHTKALIADDDVCIVGS
CCCEEEEEEECCCCCHHHHHHHHHHHHHHHHCCCEEEECCCCCEEEEEEEECCCEEEEEC
ANFDSRSFRLNFELSMLFRDQAVAAELAGMIGTDMAQAQEVRFNLRRPFWRSRLPEAFAR
CCCCCCEEEEEEEEEEEHHHHHHHHHHHHHHHCHHHHHHHHHHHHCCCHHHHHHHHHHHH
LLSPLL
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Phosphatidylglycerol [C]

Specific reaction: 2 Phosphatidylglycerol = Biphosphatidylglycerol + Glycerol. [C]

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA