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The map label for this gene is gatA [H]

Identifier: 66047205

GI number: 66047205

Start: 4728595

End: 4730409

Strand: Direct

Name: gatA [H]

Synonym: Psyr_3978

Alternate gene names: 66047205

Gene position: 4728595-4730409 (Clockwise)

Preceding gene: 66047204

Following gene: 66047206

Centisome position: 77.6

GC content: 65.29

Gene sequence:

>1815_bases
ATGAACGACACAGCACTGCCCAATGACCTGCGCCTGGACACCGTACGCACGGCCTACCAGTCCGGGCGGATATCCCCGCG
CCAGCTGATCCTGGCGCTGCGCGAAAAAGCCGCAAGGCTGAACAGCGATTATCAACTGTTCATCCACCTGCTCAGCCCGG
CTGAACTCGAGCCTTATCTGGCCGCACTGGACGATCGTGATCCCAGAGACCTGCCGCTGTTCGGCGTGCCCTTCGCGATC
AAGGACAATATTGATCTGGCGGCCATTCCAACCACCGCAGCCTGCCCGGACTACGCCTACACACCCGAGCACTCGGCAAG
CGTCGTCGCGCAACTGATCGCACTGGGTGCAGTGCCCATGGGCAAGACCAATCTGGACCAGTTCGCCACAGGCTTGAACG
GCAGCCGCTCGCCCTATGGCGCCTGCCCCAACAGCGTGTTGAACGAGTACCCGTCCGGCGGCTCGAGCGCCGGCTCGTCG
CTGGCCGTGGCCCTGGGCGTCAGCAGCTTCTCGCTGGGCACCGACACGGCGGGCTCGGGCCGAGTGCCTGCGGCACTGAA
CAACCTGGTGGGAATGAAAGCCAGCAAGGGGCTGATTTCCACGGCAGGCGTAGTACCCGCATGCCGCACACAGGATTGCG
TCTCGACCTTCACCGCCACGGCGCGGGAAGCCAGCGAGCTATTGGCACTGATCGCCGCCTTTGATCCGCGCGATGAGTAC
AGCCGTCGCAACCCGCTGTGGAACGACGCCTCGGCGTTCGGCACACCTCGCCCGTTTCGCTTCGGTGTACCCCGCGCCGA
GGACCTGCAGTTCTTTGGCTGTGCCGAGGGGCCCGGACTGTTCAGCGCAGCCATCGACCACCTCACCGCGCTGGGTGGCG
AAGCGGTCACGGTGGACTTGTCACCGTTTCTCGAAGCGGCGCGGTTGTTGTACGAGGGGCCGTGGGTGGCGGAACGCTAC
AGCGTCGCCGGGCAGTTGATGGAAGAACGCCCTGACGCGGTATTGCCGGTGATCCGCGACGTGCTGGCCAAAGCCCCCCA
GGTCAGCGGAGTCGATACGTTTCGCGCGCAATACCGCCTGCAAGCACTCAAGGCGACGTGCGATCGGGCGCTTGAAGGGC
TGGAGTGCGTAGTAACGCCAAGCATCGGCCGCCCGGTGACATCCGCAGAGCTGGCGGCCGAGCCGGTGTTGCGCAATTCG
GAGCTGGGTTACTACACGAATTTCGTCAACCTGCTCGACTACGCCGCCGTCGCCGTGCCCAGCACGTTCATGAGCAATGG
TCTGCCGTGGGGCGTGACGCTGTTCGGACGAGCGTTCACCGATCAGTACCTGCTCAGCCTGGCCGACGCCTTGCAGCGGC
AAACCGGCCTGCCCTTGATTGGCGGCGAAGCACCTCGACTGCCTGCGCCGCAGAGCACTGCGCGCAACGATATGGCGCGG
TTGGTGGTCTGCGGTGCCCATCTGGACGGACTGGCGTTGAACTGGCAGCTCAGGCAACGCGGGGCACGCCTGCTGGAAAC
CACGCAAAGCTCGGCCGACTATCGGCTGTATGCGCTGGCCGGTGGTCCGCCGTTTCGGCCCGGCATGGTGCGGGTGGCCG
AACAGGGCGTGGCCATCGACGTGGAGGTCTGGGAGTTGCCAAGTGTCGAGCTGGGATCGTTCCTGACCGGCATTCCGGCA
CCGCTCGGGCTGGGCAAGGTGCAATTGGCCGACGGCCGCTGGGAGACCGGTTTCATCTGCGAAGCCTATGGCCTGGAGGG
TGCCCGCGATATCAGCCAGCTCGGCGGCTGGCGTGCGCACCTGCAACAGCAGTAA

Upstream 100 bases:

>100_bases
TCAGGGTGCAACCCGGCTCGGCAGTACGCGCAGGGCAACGGGTGGTGGTGCTGAGCGCAGACTGAATCGAACAACTTTTC
ACTTTCAAGGGAATCCCGTC

Downstream 100 bases:

>100_bases
GCGCAAGGCGGCACCCTCGGGCGTGCCGCCTCACTCTTTCAGCGCGCTGGCGTGTGGCGATACATAGTTGTCACCTTCCC
CATCACGCTTCTCGAAATAA

Product: allophanate hydrolase

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 604; Mature: 604

Protein sequence:

>604_residues
MNDTALPNDLRLDTVRTAYQSGRISPRQLILALREKAARLNSDYQLFIHLLSPAELEPYLAALDDRDPRDLPLFGVPFAI
KDNIDLAAIPTTAACPDYAYTPEHSASVVAQLIALGAVPMGKTNLDQFATGLNGSRSPYGACPNSVLNEYPSGGSSAGSS
LAVALGVSSFSLGTDTAGSGRVPAALNNLVGMKASKGLISTAGVVPACRTQDCVSTFTATAREASELLALIAAFDPRDEY
SRRNPLWNDASAFGTPRPFRFGVPRAEDLQFFGCAEGPGLFSAAIDHLTALGGEAVTVDLSPFLEAARLLYEGPWVAERY
SVAGQLMEERPDAVLPVIRDVLAKAPQVSGVDTFRAQYRLQALKATCDRALEGLECVVTPSIGRPVTSAELAAEPVLRNS
ELGYYTNFVNLLDYAAVAVPSTFMSNGLPWGVTLFGRAFTDQYLLSLADALQRQTGLPLIGGEAPRLPAPQSTARNDMAR
LVVCGAHLDGLALNWQLRQRGARLLETTQSSADYRLYALAGGPPFRPGMVRVAEQGVAIDVEVWELPSVELGSFLTGIPA
PLGLGKVQLADGRWETGFICEAYGLEGARDISQLGGWRAHLQQQ

Sequences:

>Translated_604_residues
MNDTALPNDLRLDTVRTAYQSGRISPRQLILALREKAARLNSDYQLFIHLLSPAELEPYLAALDDRDPRDLPLFGVPFAI
KDNIDLAAIPTTAACPDYAYTPEHSASVVAQLIALGAVPMGKTNLDQFATGLNGSRSPYGACPNSVLNEYPSGGSSAGSS
LAVALGVSSFSLGTDTAGSGRVPAALNNLVGMKASKGLISTAGVVPACRTQDCVSTFTATAREASELLALIAAFDPRDEY
SRRNPLWNDASAFGTPRPFRFGVPRAEDLQFFGCAEGPGLFSAAIDHLTALGGEAVTVDLSPFLEAARLLYEGPWVAERY
SVAGQLMEERPDAVLPVIRDVLAKAPQVSGVDTFRAQYRLQALKATCDRALEGLECVVTPSIGRPVTSAELAAEPVLRNS
ELGYYTNFVNLLDYAAVAVPSTFMSNGLPWGVTLFGRAFTDQYLLSLADALQRQTGLPLIGGEAPRLPAPQSTARNDMAR
LVVCGAHLDGLALNWQLRQRGARLLETTQSSADYRLYALAGGPPFRPGMVRVAEQGVAIDVEVWELPSVELGSFLTGIPA
PLGLGKVQLADGRWETGFICEAYGLEGARDISQLGGWRAHLQQQ
>Mature_604_residues
MNDTALPNDLRLDTVRTAYQSGRISPRQLILALREKAARLNSDYQLFIHLLSPAELEPYLAALDDRDPRDLPLFGVPFAI
KDNIDLAAIPTTAACPDYAYTPEHSASVVAQLIALGAVPMGKTNLDQFATGLNGSRSPYGACPNSVLNEYPSGGSSAGSS
LAVALGVSSFSLGTDTAGSGRVPAALNNLVGMKASKGLISTAGVVPACRTQDCVSTFTATAREASELLALIAAFDPRDEY
SRRNPLWNDASAFGTPRPFRFGVPRAEDLQFFGCAEGPGLFSAAIDHLTALGGEAVTVDLSPFLEAARLLYEGPWVAERY
SVAGQLMEERPDAVLPVIRDVLAKAPQVSGVDTFRAQYRLQALKATCDRALEGLECVVTPSIGRPVTSAELAAEPVLRNS
ELGYYTNFVNLLDYAAVAVPSTFMSNGLPWGVTLFGRAFTDQYLLSLADALQRQTGLPLIGGEAPRLPAPQSTARNDMAR
LVVCGAHLDGLALNWQLRQRGARLLETTQSSADYRLYALAGGPPFRPGMVRVAEQGVAIDVEVWELPSVELGSFLTGIPA
PLGLGKVQLADGRWETGFICEAYGLEGARDISQLGGWRAHLQQQ

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=513, Percent_Identity=26.5107212475634, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI195972892, Length=166, Percent_Identity=29.5180722891566, Blast_Score=73, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI17543272, Length=410, Percent_Identity=27.0731707317073, Blast_Score=112, Evalue=5e-25,
Organism=Caenorhabditis elegans, GI71990152, Length=187, Percent_Identity=33.6898395721925, Blast_Score=86, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17556264, Length=239, Percent_Identity=28.0334728033473, Blast_Score=75, Evalue=9e-14,
Organism=Caenorhabditis elegans, GI17538252, Length=126, Percent_Identity=33.3333333333333, Blast_Score=69, Evalue=9e-12,
Organism=Saccharomyces cerevisiae, GI6319685, Length=578, Percent_Identity=42.2145328719723, Blast_Score=410, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6323950, Length=214, Percent_Identity=29.4392523364486, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24648113, Length=239, Percent_Identity=33.0543933054393, Blast_Score=80, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 64482; Mature: 64482

Theoretical pI: Translated: 4.78; Mature: 4.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDTALPNDLRLDTVRTAYQSGRISPRQLILALREKAARLNSDYQLFIHLLSPAELEPYL
CCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHH
AALDDRDPRDLPLFGVPFAIKDNIDLAAIPTTAACPDYAYTPEHSASVVAQLIALGAVPM
HHCCCCCCCCCCEECCCEEEECCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCC
GKTNLDQFATGLNGSRSPYGACPNSVLNEYPSGGSSAGSSLAVALGVSSFSLGTDTAGSG
CCCCHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCC
RVPAALNNLVGMKASKGLISTAGVVPACRTQDCVSTFTATAREASELLALIAAFDPRDEY
CCCHHHHHHHCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
SRRNPLWNDASAFGTPRPFRFGVPRAEDLQFFGCAEGPGLFSAAIDHLTALGGEAVTVDL
HHCCCCCCCHHHCCCCCCEECCCCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCEEEEEH
SPFLEAARLLYEGPWVAERYSVAGQLMEERPDAVLPVIRDVLAKAPQVSGVDTFRAQYRL
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHH
QALKATCDRALEGLECVVTPSIGRPVTSAELAAEPVLRNSELGYYTNFVNLLDYAAVAVP
HHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHC
STFMSNGLPWGVTLFGRAFTDQYLLSLADALQRQTGLPLIGGEAPRLPAPQSTARNDMAR
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCHHHHHHHEE
LVVCGAHLDGLALNWQLRQRGARLLETTQSSADYRLYALAGGPPFRPGMVRVAEQGVAID
EEEEECCCCCEEEEHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHCCCCEEE
VEVWELPSVELGSFLTGIPAPLGLGKVQLADGRWETGFICEAYGLEGARDISQLGGWRAH
EEEEECCCHHHHHHHHCCCCCCCCCEEEEECCCCCCCEEEEECCCCCHHHHHHHCCHHHH
LQQQ
HCCC
>Mature Secondary Structure
MNDTALPNDLRLDTVRTAYQSGRISPRQLILALREKAARLNSDYQLFIHLLSPAELEPYL
CCCCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCCHHH
AALDDRDPRDLPLFGVPFAIKDNIDLAAIPTTAACPDYAYTPEHSASVVAQLIALGAVPM
HHCCCCCCCCCCEECCCEEEECCCCEEECCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCC
GKTNLDQFATGLNGSRSPYGACPNSVLNEYPSGGSSAGSSLAVALGVSSFSLGTDTAGSG
CCCCHHHHHHCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCEEEEEECCCCCCCCCCCCCC
RVPAALNNLVGMKASKGLISTAGVVPACRTQDCVSTFTATAREASELLALIAAFDPRDEY
CCCHHHHHHHCCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
SRRNPLWNDASAFGTPRPFRFGVPRAEDLQFFGCAEGPGLFSAAIDHLTALGGEAVTVDL
HHCCCCCCCHHHCCCCCCEECCCCCCCCCEEEECCCCCCHHHHHHHHHHHCCCCEEEEEH
SPFLEAARLLYEGPWVAERYSVAGQLMEERPDAVLPVIRDVLAKAPQVSGVDTFRAQYRL
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHH
QALKATCDRALEGLECVVTPSIGRPVTSAELAAEPVLRNSELGYYTNFVNLLDYAAVAVP
HHHHHHHHHHHCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHC
STFMSNGLPWGVTLFGRAFTDQYLLSLADALQRQTGLPLIGGEAPRLPAPQSTARNDMAR
HHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEECCCCCCCCCCCHHHHHHHEE
LVVCGAHLDGLALNWQLRQRGARLLETTQSSADYRLYALAGGPPFRPGMVRVAEQGVAID
EEEEECCCCCEEEEHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCCCCHHHHHCCCCEEE
VEVWELPSVELGSFLTGIPAPLGLGKVQLADGRWETGFICEAYGLEGARDISQLGGWRAH
EEEEECCCHHHHHHHHCCCCCCCCCEEEEECCCCCCCEEEEECCCCCHHHHHHHCCHHHH
LQQQ
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11932238 [H]