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The map label for this gene is phnD [H]

Identifier: 66045488

GI number: 66045488

Start: 2614437

End: 2615447

Strand: Direct

Name: phnD [H]

Synonym: Psyr_2248

Alternate gene names: 66045488

Gene position: 2614437-2615447 (Clockwise)

Preceding gene: 66045487

Following gene: 66045489

Centisome position: 42.9

GC content: 58.06

Gene sequence:

>1011_bases
ATGTTCAAGCGTATCGGTCGTCTTCTCGCCTCTGCCGCCTTGCTGACTGCCTGCGCGTTGGGCACGGTCCAGGCCGCAGA
AGAAAAAGCCATCAATTTCGGCATCATGTCCACCGAGTCTTCGCAAAACCTGAAAAGTATCTGGCAGCCGTTTCTCGATG
ACATGAGCCGCAAGACCGGTCTTAAGGTCAACGCCACCTTCGCCTCGGACTACGCCGGCCTGATTCAGGGCATGCGCTTC
AACAAGGTGGACGTGGCCTGGCTGGGCAACAAGGCGGCGATGGAAGCGGTGGACCGCTCCAACGGCGAGATCTTTGCCCA
GACCTCGGCCGCCAACGGTGACGCGGGCTACTGGAGCCTGCTGATCGTGCGCAAGGACAGCCCGATCAATAGCGTCGAAG
ACATGCTCAAGAACGCCAGGAGCCTGACCTTCGGCAACGGTGACCCGAACTCCACTTCGGGCTATCTGGTGCCAGGTTAT
TACGTGTTTGCGAAAAACCACGTCGATGCGTCCACCGCCTTCAAGCGCACCTTGAACTCCAGTCATGAAGTCAACGCCCT
CAGTGTTGCCAAGGGTCAGCTGGACGTTGCGACCTTCAACACCGAAAGCTGGGATCGCCTGGCCGTGACCCAGCCCGACA
AGGTTGAGCAACTCAAGGTGATCTGGAAATCGCCGCTGATCCCGGCCGACCCGATTGTCTGGAGCAAGGCGCTGTCGGAC
CAGGACAAGGCCAGGATTCGTGAATTCTTCACCAGTTATGGCGATACCGATGAAGAGAAAACCGTCCTGAAGAACATGCA
AATGGGCAAGTTCCTCAAGTCCAGCGACGACCAGTTGTTGCCCATTCGCCAGCTCGAACTGTTCAAGCAGCGCACCGAAG
TGGTGGCCAGTACCACGCTGGATGAGCAGGAGAAAGCCACCCGTCTCAAGGAGATCGATGCCAGCCTGAGCAAGTTGCAG
GAGCGTATCACCGAGCTGAACCAGAAAACCGCAGGCAGCTCCGCAGGTTGA

Upstream 100 bases:

>100_bases
CCGAGCGAAGCCAGCGCGTGCGGCAAAAAGCCCGCCTCACGCTGGCCAAGGTCTGACGTCGCTTCAACATCCCCCACCTG
ACATTTTCCGGAGAGCCTGC

Downstream 100 bases:

>100_bases
GCCAGCCGTTCACGATCAGGAGTTCGTCATGACCACTCACGTTGCCTATACCCAGGTTGCGGGCAAACCCAACAGGTCCC
GTTATCTGGGCTGGGGCCTG

Product: phosphonate-binding periplasmic protein

Products: 3-phospho-serine [Cytoplasm]; phosphate [Cytoplasm]; alkylphosphonate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 336; Mature: 336

Protein sequence:

>336_residues
MFKRIGRLLASAALLTACALGTVQAAEEKAINFGIMSTESSQNLKSIWQPFLDDMSRKTGLKVNATFASDYAGLIQGMRF
NKVDVAWLGNKAAMEAVDRSNGEIFAQTSAANGDAGYWSLLIVRKDSPINSVEDMLKNARSLTFGNGDPNSTSGYLVPGY
YVFAKNHVDASTAFKRTLNSSHEVNALSVAKGQLDVATFNTESWDRLAVTQPDKVEQLKVIWKSPLIPADPIVWSKALSD
QDKARIREFFTSYGDTDEEKTVLKNMQMGKFLKSSDDQLLPIRQLELFKQRTEVVASTTLDEQEKATRLKEIDASLSKLQ
ERITELNQKTAGSSAG

Sequences:

>Translated_336_residues
MFKRIGRLLASAALLTACALGTVQAAEEKAINFGIMSTESSQNLKSIWQPFLDDMSRKTGLKVNATFASDYAGLIQGMRF
NKVDVAWLGNKAAMEAVDRSNGEIFAQTSAANGDAGYWSLLIVRKDSPINSVEDMLKNARSLTFGNGDPNSTSGYLVPGY
YVFAKNHVDASTAFKRTLNSSHEVNALSVAKGQLDVATFNTESWDRLAVTQPDKVEQLKVIWKSPLIPADPIVWSKALSD
QDKARIREFFTSYGDTDEEKTVLKNMQMGKFLKSSDDQLLPIRQLELFKQRTEVVASTTLDEQEKATRLKEIDASLSKLQ
ERITELNQKTAGSSAG
>Mature_336_residues
MFKRIGRLLASAALLTACALGTVQAAEEKAINFGIMSTESSQNLKSIWQPFLDDMSRKTGLKVNATFASDYAGLIQGMRF
NKVDVAWLGNKAAMEAVDRSNGEIFAQTSAANGDAGYWSLLIVRKDSPINSVEDMLKNARSLTFGNGDPNSTSGYLVPGY
YVFAKNHVDASTAFKRTLNSSHEVNALSVAKGQLDVATFNTESWDRLAVTQPDKVEQLKVIWKSPLIPADPIVWSKALSD
QDKARIREFFTSYGDTDEEKTVLKNMQMGKFLKSSDDQLLPIRQLELFKQRTEVVASTTLDEQEKATRLKEIDASLSKLQ
ERITELNQKTAGSSAG

Specific function: Part of the binding-protein-dependent transport system for alkylphosphonates [H]

COG id: COG3221

COG function: function code P; ABC-type phosphate/phosphonate transport system, periplasmic component

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790543, Length=319, Percent_Identity=58.9341692789969, Blast_Score=384, Evalue=1e-108,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017797
- InterPro:   IPR005770 [H]

Pfam domain/function: NA

EC number: NA

Molecular weight: Translated: 37036; Mature: 37036

Theoretical pI: Translated: 7.50; Mature: 7.50

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKRIGRLLASAALLTACALGTVQAAEEKAINFGIMSTESSQNLKSIWQPFLDDMSRKTG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHCC
LKVNATFASDYAGLIQGMRFNKVDVAWLGNKAAMEAVDRSNGEIFAQTSAANGDAGYWSL
CEEEEEHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHCCCCEEEEEECCCCCCCCEEEE
LIVRKDSPINSVEDMLKNARSLTFGNGDPNSTSGYLVPGYYVFAKNHVDASTAFKRTLNS
EEEECCCCCHHHHHHHHHHHEEECCCCCCCCCCCEEECCEEEEEECCCCHHHHHHHHHCC
SHEVNALSVAKGQLDVATFNTESWDRLAVTQPDKVEQLKVIWKSPLIPADPIVWSKALSD
CCCCCEEEEECCCEEEEEECCCCCCEEECCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCC
QDKARIREFFTSYGDTDEEKTVLKNMQMGKFLKSSDDQLLPIRQLELFKQRTEVVASTTL
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCC
DEQEKATRLKEIDASLSKLQERITELNQKTAGSSAG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MFKRIGRLLASAALLTACALGTVQAAEEKAINFGIMSTESSQNLKSIWQPFLDDMSRKTG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCHHHHHHHHHHHHHHHHHCC
LKVNATFASDYAGLIQGMRFNKVDVAWLGNKAAMEAVDRSNGEIFAQTSAANGDAGYWSL
CEEEEEHHHHHHHHHCCCCCCCEEEEECCCHHHHHHHHCCCCEEEEEECCCCCCCCEEEE
LIVRKDSPINSVEDMLKNARSLTFGNGDPNSTSGYLVPGYYVFAKNHVDASTAFKRTLNS
EEEECCCCCHHHHHHHHHHHEEECCCCCCCCCCCEEECCEEEEEECCCCHHHHHHHHHCC
SHEVNALSVAKGQLDVATFNTESWDRLAVTQPDKVEQLKVIWKSPLIPADPIVWSKALSD
CCCCCEEEEECCCEEEEEECCCCCCEEECCCCCHHHHHHHHHHCCCCCCCCHHHHHHHCC
QDKARIREFFTSYGDTDEEKTVLKNMQMGKFLKSSDDQLLPIRQLELFKQRTEVVASTTL
HHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCC
DEQEKATRLKEIDASLSKLQERITELNQKTAGSSAG
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; 3-phospho-serine [Periplasm]; phosphite [Periplasm]; H2O; phosphate [Periplasm]; alkylphosphonate [Periplasm] [C]

Specific reaction: H2O + ATP + 3-phospho-serine [Periplasm] = ADP + phosphate + 3-phospho-serine [Cytoplasm] ATP + phosphite [Periplasm] + H2O = phosphite [Cytoplasm] + ADP + phosphate phosphate [Periplasm] + ATP + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] A

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2155230; 1840580; 7610040; 9278503 [H]