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The map label for this gene is pleD [H]

Identifier: 66045350

GI number: 66045350

Start: 2464720

End: 2467110

Strand: Direct

Name: pleD [H]

Synonym: Psyr_2110

Alternate gene names: 66045350

Gene position: 2464720-2467110 (Clockwise)

Preceding gene: 66045349

Following gene: 66045352

Centisome position: 40.45

GC content: 60.6

Gene sequence:

>2391_bases
ATGACAGTGAAACGGTTCAAGCTCAGAGTTCTGAGTTTCATCAATGGCCAGCTTTCAGCGTGGCTTATCGCTGTCGTGGC
ATTGCTGGTCGGTGTGGCACTTACGATCATGCTGGCGCTGGCCGACAACGAGTTGTATCAGCGCCAGTTGCGTCAGCGTT
TCGATACTGTTGCCGCGGAGCGCTTCAGTCGCCTGCAGGAGCGTCTGGACCGGCAGACTACCCGGCTCGACACCCTCGCT
CGCTTCTTTATCTACTCCCATCAGGTTGAACTGTCCGAATTCAACGGCTTTGTCGCGCCTTTACTGATTGGTACCCAGGC
GTATGCCTGGAACCCTAGAGTGTCGCAGGCCGAACGGACGGCTTTTGAAAGACAGTTGCGTGAGGAAGGCTCAGCCGCGT
ATGCCATAAGCGAAATGGATGAAAACGGCGCCTTGAAAGCCGCCGCCGTGCGAAATGAGTATTTCCCGGTTCGATTCATC
CAGACGCTCAGTGACATTCCGACGCCAGCAGGGTTCGATATCGCCTCGGAGCCGGTACGCCGTGCCGCGCTGGAGCGTGC
GCGGTTGCTGAAACGAACCGTCGCAACGCCTCGTATCCGCCTGCTTTCCCTGGAGCCGTCGGACACGTATGGCATTCTGC
TGGTCGCGCCGGTGTTCTCGACTGGCCAGCCGGTTCCACAGTTGCGGGGCTATGTCACCGCCGTGATCAGCCTGACGCAA
TTGATGAATGTGCGCACGGCAGAGCAGGACAATCTTGCCGTGACCATGCTCGACCTCAGTTCGCCGGAAAAGCCCGAGCA
GGTTTATCAGTCGCCGCTCCCTGCGATACACAATCAGTTGTATGCCAGCAGCCTGCTGAACCTGGGTGACAGGGATTATC
TGGTCGAGGTTCGCCCTACGCAGATTTTCAGCCTGAACAACCAGATCATGATGCCCGGCCGGGTGTTGTGGCTTGGAGGG
CTTTTGAGCCTGATGCTCAGCGCCTTGCTCTACAGTCTGATCAGCCAGCGTCAGCGCGCCTTGCAACGGGTGGCGCAGCG
CACCCGCGAGCTGCGCCAGCGCGAGCACCAATTGAGGGCCGCGCATGGGCAGTTGCGCAGTGTGCTGGACGCGGCGACCG
AAGTGGCGATCATCGCCACCGATCTGGACGGGCTGATCAAGACGTTCAACGTCGGAGCACAGAAAATGCTCGGCTATGCG
CAAGAGGATGTGCTGGGCAGGTTCCGGCTGATGGACTTTTATCAGTCTGCCGATCTGGAGGCCCGCGCGAGCCGCATGAG
CAAGAGCCGCGGGCAACCCATCAGCGCCTCGCAGCTGATGTTTCTCGATGCTGTGCAGGATGGTAATCACCCTTCGCAGG
AGTGGACCCTGCAGCGCAGCGATGGCAGCACGCTGGTGGTGAACATGCTGGTCACTGCGGTGCGTGACGATCAGGGGCTG
TGGACCGGGTATCTGGCGGTATGCATCGACGTCACCGAGCACAAGCGCGTCAACCAGGCCCTTGCCGAGCAGGGACAGTT
GTTGAAAAAGCTCGGCTCGCAAGTGCCTGGCGGCATTTACCAGTACCACCTGGCCGTCGATGGCAGCGCATGTTTCAGGT
ACGCCAGCGCCGGCATGTGCGAGCTGTTCGAGGTGGACGAAGAGCAGTTGCTGGGCGATTCCGAAACTATCCTGCAACGC
ATACAGCCGCTGGACCTGGCACGGGTCAGAAGCTCCATTCTGATCAGCGCCACACACCTGACACCGTGGAGCCAGGAGTA
CCGCGTCAATCTGCCACGCAAGGGGCAGCGCTGGTTACGCTCGGCCTCCACGCCCGAGCGCCTGGCCGATGGCAGCGTGC
TGTGGCATGGCTTTGTGTCCGATATCACCGACCTCAAACGCGTCGAGCAGGAGTTGCGTGCGCTGTCGGTCACCGACGTG
CTGACCGGCGCCTATAATCGTCGCTACTTTCAGGACCGGATGCAGGCCGAGCTGAAAAGAATCGACCGGCACGGCGGTAA
CCTGTCGATCATCATGCTCGATATCGATCACTTCAAGCGGATCAATGACCGCTTCGGGCATGCCGCAGGCGATCAGGTGC
TCAAGGCCATCTCGGAAAAGATCAGCCAGCGGCTGCGCAGCGACGATGTGTTCTGCCGTCTGGGTGGCGAAGAGTTTATG
GTGATCTGTCCGGGAACGCGGGGTGCCCAGGCCTATCAACTGGCACTGAGCCTGCGCGAGGAACTGCGCAATCAGGCTAT
CGAAGGCGTCGACCAAGTGGTCACAGGCAGCTTCGGGATCGCCAGCTGGCGAGAGGGTGAGGGGATCGACGAGATGTTGC
TGCGTGCCGACTCGGGCGTCTACGCCGCCAAACAGGCCGGTCGCGACCGGGTCGAGCCCGAGCAGGGTTGA

Upstream 100 bases:

>100_bases
TGAAACAGGCATAAGCCTATGAAAATTATTGGCGAATAGCTATAACAGAAGTCATAGCCAGCCTGACGCTTCCGTATGTT
GGCGTCATGAGTTTGTGTGT

Downstream 100 bases:

>100_bases
TGCAGACAGACCCGCTGCGATAGCGGGTCTGCCGATGGCCGGTATCGGCTGGTTATTGCTGGATCGAGACGGTGGTGTTG
GTCAGCTTGGGCTGCCGGTA

Product: PAS:GGDEF

Products: NA

Alternate protein names: Stalked cell differentiation-controlling protein; Diguanylate cyclase; DGC; Diguanylate kinase [H]

Number of amino acids: Translated: 796; Mature: 795

Protein sequence:

>796_residues
MTVKRFKLRVLSFINGQLSAWLIAVVALLVGVALTIMLALADNELYQRQLRQRFDTVAAERFSRLQERLDRQTTRLDTLA
RFFIYSHQVELSEFNGFVAPLLIGTQAYAWNPRVSQAERTAFERQLREEGSAAYAISEMDENGALKAAAVRNEYFPVRFI
QTLSDIPTPAGFDIASEPVRRAALERARLLKRTVATPRIRLLSLEPSDTYGILLVAPVFSTGQPVPQLRGYVTAVISLTQ
LMNVRTAEQDNLAVTMLDLSSPEKPEQVYQSPLPAIHNQLYASSLLNLGDRDYLVEVRPTQIFSLNNQIMMPGRVLWLGG
LLSLMLSALLYSLISQRQRALQRVAQRTRELRQREHQLRAAHGQLRSVLDAATEVAIIATDLDGLIKTFNVGAQKMLGYA
QEDVLGRFRLMDFYQSADLEARASRMSKSRGQPISASQLMFLDAVQDGNHPSQEWTLQRSDGSTLVVNMLVTAVRDDQGL
WTGYLAVCIDVTEHKRVNQALAEQGQLLKKLGSQVPGGIYQYHLAVDGSACFRYASAGMCELFEVDEEQLLGDSETILQR
IQPLDLARVRSSILISATHLTPWSQEYRVNLPRKGQRWLRSASTPERLADGSVLWHGFVSDITDLKRVEQELRALSVTDV
LTGAYNRRYFQDRMQAELKRIDRHGGNLSIIMLDIDHFKRINDRFGHAAGDQVLKAISEKISQRLRSDDVFCRLGGEEFM
VICPGTRGAQAYQLALSLREELRNQAIEGVDQVVTGSFGIASWREGEGIDEMLLRADSGVYAAKQAGRDRVEPEQG

Sequences:

>Translated_796_residues
MTVKRFKLRVLSFINGQLSAWLIAVVALLVGVALTIMLALADNELYQRQLRQRFDTVAAERFSRLQERLDRQTTRLDTLA
RFFIYSHQVELSEFNGFVAPLLIGTQAYAWNPRVSQAERTAFERQLREEGSAAYAISEMDENGALKAAAVRNEYFPVRFI
QTLSDIPTPAGFDIASEPVRRAALERARLLKRTVATPRIRLLSLEPSDTYGILLVAPVFSTGQPVPQLRGYVTAVISLTQ
LMNVRTAEQDNLAVTMLDLSSPEKPEQVYQSPLPAIHNQLYASSLLNLGDRDYLVEVRPTQIFSLNNQIMMPGRVLWLGG
LLSLMLSALLYSLISQRQRALQRVAQRTRELRQREHQLRAAHGQLRSVLDAATEVAIIATDLDGLIKTFNVGAQKMLGYA
QEDVLGRFRLMDFYQSADLEARASRMSKSRGQPISASQLMFLDAVQDGNHPSQEWTLQRSDGSTLVVNMLVTAVRDDQGL
WTGYLAVCIDVTEHKRVNQALAEQGQLLKKLGSQVPGGIYQYHLAVDGSACFRYASAGMCELFEVDEEQLLGDSETILQR
IQPLDLARVRSSILISATHLTPWSQEYRVNLPRKGQRWLRSASTPERLADGSVLWHGFVSDITDLKRVEQELRALSVTDV
LTGAYNRRYFQDRMQAELKRIDRHGGNLSIIMLDIDHFKRINDRFGHAAGDQVLKAISEKISQRLRSDDVFCRLGGEEFM
VICPGTRGAQAYQLALSLREELRNQAIEGVDQVVTGSFGIASWREGEGIDEMLLRADSGVYAAKQAGRDRVEPEQG
>Mature_795_residues
TVKRFKLRVLSFINGQLSAWLIAVVALLVGVALTIMLALADNELYQRQLRQRFDTVAAERFSRLQERLDRQTTRLDTLAR
FFIYSHQVELSEFNGFVAPLLIGTQAYAWNPRVSQAERTAFERQLREEGSAAYAISEMDENGALKAAAVRNEYFPVRFIQ
TLSDIPTPAGFDIASEPVRRAALERARLLKRTVATPRIRLLSLEPSDTYGILLVAPVFSTGQPVPQLRGYVTAVISLTQL
MNVRTAEQDNLAVTMLDLSSPEKPEQVYQSPLPAIHNQLYASSLLNLGDRDYLVEVRPTQIFSLNNQIMMPGRVLWLGGL
LSLMLSALLYSLISQRQRALQRVAQRTRELRQREHQLRAAHGQLRSVLDAATEVAIIATDLDGLIKTFNVGAQKMLGYAQ
EDVLGRFRLMDFYQSADLEARASRMSKSRGQPISASQLMFLDAVQDGNHPSQEWTLQRSDGSTLVVNMLVTAVRDDQGLW
TGYLAVCIDVTEHKRVNQALAEQGQLLKKLGSQVPGGIYQYHLAVDGSACFRYASAGMCELFEVDEEQLLGDSETILQRI
QPLDLARVRSSILISATHLTPWSQEYRVNLPRKGQRWLRSASTPERLADGSVLWHGFVSDITDLKRVEQELRALSVTDVL
TGAYNRRYFQDRMQAELKRIDRHGGNLSIIMLDIDHFKRINDRFGHAAGDQVLKAISEKISQRLRSDDVFCRLGGEEFMV
ICPGTRGAQAYQLALSLREELRNQAIEGVDQVVTGSFGIASWREGEGIDEMLLRADSGVYAAKQAGRDRVEPEQG

Specific function: Catalyzes the condensation of two GTP molecules to the cyclic dinucleotide di-GMP (c-di-GMP), which acts as a secondary messenger [H]

COG id: COG2199

COG function: function code T; FOG: GGDEF domain

Gene ontology:

Cell location: Cytoplasm. Note=Phosphorylated PleD localizes to the differentiating pole [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 response regulatory domains [H]

Homologues:

Organism=Escherichia coli, GI1786584, Length=180, Percent_Identity=36.6666666666667, Blast_Score=113, Evalue=4e-26,
Organism=Escherichia coli, GI87081881, Length=181, Percent_Identity=35.9116022099448, Blast_Score=110, Evalue=3e-25,
Organism=Escherichia coli, GI87082007, Length=184, Percent_Identity=35.8695652173913, Blast_Score=105, Evalue=8e-24,
Organism=Escherichia coli, GI145693134, Length=175, Percent_Identity=32.5714285714286, Blast_Score=98, Evalue=2e-21,
Organism=Escherichia coli, GI1787541, Length=171, Percent_Identity=35.0877192982456, Blast_Score=97, Evalue=3e-21,
Organism=Escherichia coli, GI1787262, Length=158, Percent_Identity=32.9113924050633, Blast_Score=94, Evalue=3e-20,
Organism=Escherichia coli, GI1788381, Length=321, Percent_Identity=26.4797507788162, Blast_Score=94, Evalue=4e-20,
Organism=Escherichia coli, GI1788085, Length=177, Percent_Identity=33.3333333333333, Blast_Score=86, Evalue=7e-18,
Organism=Escherichia coli, GI1787816, Length=177, Percent_Identity=32.2033898305085, Blast_Score=84, Evalue=5e-17,
Organism=Escherichia coli, GI87081974, Length=164, Percent_Identity=28.0487804878049, Blast_Score=82, Evalue=1e-16,
Organism=Escherichia coli, GI1787802, Length=159, Percent_Identity=29.559748427673, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI87081977, Length=168, Percent_Identity=33.3333333333333, Blast_Score=75, Evalue=2e-14,
Organism=Escherichia coli, GI1787056, Length=188, Percent_Identity=28.7234042553192, Blast_Score=72, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001054
- InterPro:   IPR011006
- InterPro:   IPR000160
- InterPro:   IPR001789 [H]

Pfam domain/function: PF00990 GGDEF; PF00072 Response_reg [H]

EC number: =2.7.7.65 [H]

Molecular weight: Translated: 89368; Mature: 89237

Theoretical pI: Translated: 7.65; Mature: 7.65

Prosite motif: PS50839 CHASE ; PS50112 PAS ; PS50113 PAC ; PS50887 GGDEF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTVKRFKLRVLSFINGQLSAWLIAVVALLVGVALTIMLALADNELYQRQLRQRFDTVAAE
CCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
RFSRLQERLDRQTTRLDTLARFFIYSHQVELSEFNGFVAPLLIGTQAYAWNPRVSQAERT
HHHHHHHHHHHHHHHHHHHHHHHHHHCCEEHHHCCCHHHHHHHCCCCEECCCCCCHHHHH
AFERQLREEGSAAYAISEMDENGALKAAAVRNEYFPVRFIQTLSDIPTPAGFDIASEPVR
HHHHHHHHCCCCEEEEEECCCCCCEEHHHHHCCCCHHHHHHHHHHCCCCCCCCCHHHHHH
RAALERARLLKRTVATPRIRLLSLEPSDTYGILLVAPVFSTGQPVPQLRGYVTAVISLTQ
HHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHH
LMNVRTAEQDNLAVTMLDLSSPEKPEQVYQSPLPAIHNQLYASSLLNLGDRDYLVEVRPT
HHHHHCCCCCCEEEEEEECCCCCCHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEECCC
QIFSLNNQIMMPGRVLWLGGLLSLMLSALLYSLISQRQRALQRVAQRTRELRQREHQLRA
EEEECCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AHGQLRSVLDAATEVAIIATDLDGLIKTFNVGAQKMLGYAQEDVLGRFRLMDFYQSADLE
HHHHHHHHHHHHHHHEEEEECHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHH
ARASRMSKSRGQPISASQLMFLDAVQDGNHPSQEWTLQRSDGSTLVVNMLVTAVRDDQGL
HHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCEEECCCCCCHHHHHHHHHHHCCCCH
WTGYLAVCIDVTEHKRVNQALAEQGQLLKKLGSQVPGGIYQYHLAVDGSACFRYASAGMC
HHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECHHHHHHHHCCCCH
ELFEVDEEQLLGDSETILQRIQPLDLARVRSSILISATHLTPWSQEYRVNLPRKGQRWLR
HHHHCCHHHHCCCHHHHHHHCCCCHHHHHHHHHEEEEECCCCCCHHHEECCCCHHHHHHH
SASTPERLADGSVLWHGFVSDITDLKRVEQELRALSVTDVLTGAYNRRYFQDRMQAELKR
CCCCHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IDRHGGNLSIIMLDIDHFKRINDRFGHAAGDQVLKAISEKISQRLRSDDVFCRLGGEEFM
HHHCCCCEEEEEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEE
VICPGTRGAQAYQLALSLREELRNQAIEGVDQVVTGSFGIASWREGEGIDEMLLRADSGV
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCC
YAAKQAGRDRVEPEQG
HHHHHCCCCCCCCCCC
>Mature Secondary Structure 
TVKRFKLRVLSFINGQLSAWLIAVVALLVGVALTIMLALADNELYQRQLRQRFDTVAAE
CHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
RFSRLQERLDRQTTRLDTLARFFIYSHQVELSEFNGFVAPLLIGTQAYAWNPRVSQAERT
HHHHHHHHHHHHHHHHHHHHHHHHHHCCEEHHHCCCHHHHHHHCCCCEECCCCCCHHHHH
AFERQLREEGSAAYAISEMDENGALKAAAVRNEYFPVRFIQTLSDIPTPAGFDIASEPVR
HHHHHHHHCCCCEEEEEECCCCCCEEHHHHHCCCCHHHHHHHHHHCCCCCCCCCHHHHHH
RAALERARLLKRTVATPRIRLLSLEPSDTYGILLVAPVFSTGQPVPQLRGYVTAVISLTQ
HHHHHHHHHHHHHHCCCCEEEEEECCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHH
LMNVRTAEQDNLAVTMLDLSSPEKPEQVYQSPLPAIHNQLYASSLLNLGDRDYLVEVRPT
HHHHHCCCCCCEEEEEEECCCCCCHHHHHHCCCHHHHHHHHHHHHHCCCCCCEEEEECCC
QIFSLNNQIMMPGRVLWLGGLLSLMLSALLYSLISQRQRALQRVAQRTRELRQREHQLRA
EEEECCCCEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AHGQLRSVLDAATEVAIIATDLDGLIKTFNVGAQKMLGYAQEDVLGRFRLMDFYQSADLE
HHHHHHHHHHHHHHHEEEEECHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHCCCHH
ARASRMSKSRGQPISASQLMFLDAVQDGNHPSQEWTLQRSDGSTLVVNMLVTAVRDDQGL
HHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCCCCEEECCCCCCHHHHHHHHHHHCCCCH
WTGYLAVCIDVTEHKRVNQALAEQGQLLKKLGSQVPGGIYQYHLAVDGSACFRYASAGMC
HHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEEEECHHHHHHHHCCCCH
ELFEVDEEQLLGDSETILQRIQPLDLARVRSSILISATHLTPWSQEYRVNLPRKGQRWLR
HHHHCCHHHHCCCHHHHHHHCCCCHHHHHHHHHEEEEECCCCCCHHHEECCCCHHHHHHH
SASTPERLADGSVLWHGFVSDITDLKRVEQELRALSVTDVLTGAYNRRYFQDRMQAELKR
CCCCHHHHCCCCCEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IDRHGGNLSIIMLDIDHFKRINDRFGHAAGDQVLKAISEKISQRLRSDDVFCRLGGEEFM
HHHCCCCEEEEEEEHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHCCCCEEEEECCCEEE
VICPGTRGAQAYQLALSLREELRNQAIEGVDQVVTGSFGIASWREGEGIDEMLLRADSGV
EECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCCCC
YAAKQAGRDRVEPEQG
HHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA