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The map label for this gene is mii [H]

Identifier: 66045328

GI number: 66045328

Start: 2435367

End: 2436557

Strand: Direct

Name: mii [H]

Synonym: Psyr_2088

Alternate gene names: 66045328

Gene position: 2435367-2436557 (Clockwise)

Preceding gene: 66045327

Following gene: 66045330

Centisome position: 39.97

GC content: 65.74

Gene sequence:

>1191_bases
ATGGCTCACCCACCACAGATCCGCATCCCCGCCACTTACATGCGCGGCGGCACCAGCAAGGGGGTGTTTTTCAGGCTGAC
TGATCTGCCCGAAGCTGCACAGGTTCCGGGCGCGGCGCGGGATGCGCTGCTGTTGCGGGTCATCGGCAGCCCTGACCCCT
ATGAAAAGCAGATCGACGGCATGGGCGGGGCGACGTCGAGTACCAGCAAGAGCGTGATTGTGTCGCGCAGCAGCAGGCCC
GACCACGATGTCGATTACCTGTTCGGCCAGGTGTCCATCGACAAGCCGTTTGTCGACTGGAGCGGCAATTGCGGCAACCT
GTCAGCGGCCGTGGGGCCGTTCGCGATTACGAGTGGCTTGGTCGACCCGGGCCGTGTTCCGCAGGACGGCGTCGCGGTGG
TGCGCATCTGGCAGGCCAACATCGGCAAGACCATCATCGCTCACGTGCCGGTCAGCAACGGCGCGGTGCAGGAAACCGGC
GACTTCGAGCTGGATGGCGTGACCTTCCCGGCAGCGGAAGTGCCACTGGAGTTTCTTGATCCGGCGGCGGACGAAGAGGG
CGCGGGTGGCTCGATGTTTCCCACCGGCAATCTGGTGGATGATCTGCAGGTGCCCGGCATCGGCACTTTGAAGGCGACCA
TGATCAATGCCGGCATCCCGACGATTTTCGTCAACGCCGCCGACATCGGCTATACCGGCACCGAACTGCAGGGCGATATC
AACGCTGATCCGCAGGCGCTGGCCCGTCTGGAGGCCATTCGCGCCTATGGTGCGCTGCACATGGGCCTGATTGGCAGCAT
TGATGAAGCTGCCGCACGCCAGCACACGCCCAAGGTGGCTTTTGTCGCTGCGCCTGCCGACTACCTGGCGTCGAGCGGCA
AGCCTGTCGCGGCGCAGGATATCGAGCTGCTGGTACGCGCCGTGTCGATGGGCAAGCTGCACCACGCGATGATGGGCACG
GCTGCGGTGGCCATCGGCACAGCGGCGGCCATCCCGGGCACGCTGGTCAATCTGGCCGCTGGTGGTCAGGCGCGCAGCGC
AGTCAGCTTTGGCCACCCCTCCGGCACACTGCGGGTCGGCGCCCAGGCCGTTCAGGAGGGCGGTGACTGGAAAGTCACCA
AGGCCCTGATGAGCCGCAGCGCCCGCGTGCTGATGGAAGGCTGGGTGCGGGTGCCGCAGCCAGGCGTCTGA

Upstream 100 bases:

>100_bases
GAGCACTATCGTGCCCATGCTCCGCGTGGGCATGCAGTTCTGGACGCTCTGCGTCCGATTTTGAGCGCGCAGCGCGCTCT
TTGAACAGGACAACGAATTC

Downstream 100 bases:

>100_bases
GTCGTCGGTTTGACGGAATGACAGCAGCGTCTACTTGAAACGTCGCTCCACGCCTTTTTCGACCAGCACCTTGGCGGAGA
TTTCTTCCACCGAAAAATGC

Product: hypothetical protein

Products: NA

Alternate protein names: (R)-3-methylitaconate isomerase; 3-methylitaconate delta-isomerase [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MAHPPQIRIPATYMRGGTSKGVFFRLTDLPEAAQVPGAARDALLLRVIGSPDPYEKQIDGMGGATSSTSKSVIVSRSSRP
DHDVDYLFGQVSIDKPFVDWSGNCGNLSAAVGPFAITSGLVDPGRVPQDGVAVVRIWQANIGKTIIAHVPVSNGAVQETG
DFELDGVTFPAAEVPLEFLDPAADEEGAGGSMFPTGNLVDDLQVPGIGTLKATMINAGIPTIFVNAADIGYTGTELQGDI
NADPQALARLEAIRAYGALHMGLIGSIDEAAARQHTPKVAFVAAPADYLASSGKPVAAQDIELLVRAVSMGKLHHAMMGT
AAVAIGTAAAIPGTLVNLAAGGQARSAVSFGHPSGTLRVGAQAVQEGGDWKVTKALMSRSARVLMEGWVRVPQPGV

Sequences:

>Translated_396_residues
MAHPPQIRIPATYMRGGTSKGVFFRLTDLPEAAQVPGAARDALLLRVIGSPDPYEKQIDGMGGATSSTSKSVIVSRSSRP
DHDVDYLFGQVSIDKPFVDWSGNCGNLSAAVGPFAITSGLVDPGRVPQDGVAVVRIWQANIGKTIIAHVPVSNGAVQETG
DFELDGVTFPAAEVPLEFLDPAADEEGAGGSMFPTGNLVDDLQVPGIGTLKATMINAGIPTIFVNAADIGYTGTELQGDI
NADPQALARLEAIRAYGALHMGLIGSIDEAAARQHTPKVAFVAAPADYLASSGKPVAAQDIELLVRAVSMGKLHHAMMGT
AAVAIGTAAAIPGTLVNLAAGGQARSAVSFGHPSGTLRVGAQAVQEGGDWKVTKALMSRSARVLMEGWVRVPQPGV
>Mature_395_residues
AHPPQIRIPATYMRGGTSKGVFFRLTDLPEAAQVPGAARDALLLRVIGSPDPYEKQIDGMGGATSSTSKSVIVSRSSRPD
HDVDYLFGQVSIDKPFVDWSGNCGNLSAAVGPFAITSGLVDPGRVPQDGVAVVRIWQANIGKTIIAHVPVSNGAVQETGD
FELDGVTFPAAEVPLEFLDPAADEEGAGGSMFPTGNLVDDLQVPGIGTLKATMINAGIPTIFVNAADIGYTGTELQGDIN
ADPQALARLEAIRAYGALHMGLIGSIDEAAARQHTPKVAFVAAPADYLASSGKPVAAQDIELLVRAVSMGKLHHAMMGTA
AVAIGTAAAIPGTLVNLAAGGQARSAVSFGHPSGTLRVGAQAVQEGGDWKVTKALMSRSARVLMEGWVRVPQPGV

Specific function: Catalyzes the reversible isomerization of (R)-3- methylitaconate to 2,3-dimethylmaleate. Has very low isomerase activity with itaconate [H]

COG id: COG2828

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1786985, Length=385, Percent_Identity=34.8051948051948, Blast_Score=181, Evalue=8e-47,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007400 [H]

Pfam domain/function: PF04303 PrpF [H]

EC number: =5.3.3.6 [H]

Molecular weight: Translated: 40899; Mature: 40768

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAHPPQIRIPATYMRGGTSKGVFFRLTDLPEAAQVPGAARDALLLRVIGSPDPYEKQIDG
CCCCCCEECCHHHHCCCCCCCEEEEEECCCHHHHCCCCHHCEEEEEECCCCCCHHHHHCC
MGGATSSTSKSVIVSRSSRPDHDVDYLFGQVSIDKPFVDWSGNCGNLSAAVGPFAITSGL
CCCCCCCCCCEEEEECCCCCCCCHHHEEEEEEECCCCCCCCCCCCCCCHHHCCHHHHHCC
VDPGRVPQDGVAVVRIWQANIGKTIIAHVPVSNGAVQETGDFELDGVTFPAAEVPLEFLD
CCCCCCCCCCEEEEEEEECCCCCEEEEEECCCCCCCCCCCCEEECCEECCHHHCCHHHHC
PAADEEGAGGSMFPTGNLVDDLQVPGIGTLKATMINAGIPTIFVNAADIGYTGTELQGDI
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHEEECCCCCEEEEECCCCCCCCCCCCCCC
NADPQALARLEAIRAYGALHMGLIGSIDEAAARQHTPKVAFVAAPADYLASSGKPVAAQD
CCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEECCHHHHHCCCCCCHHHH
IELLVRAVSMGKLHHAMMGTAAVAIGTAAAIPGTLVNLAAGGQARSAVSFGHPSGTLRVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEECCCCCCCEEECCCCCCCEEEC
AQAVQEGGDWKVTKALMSRSARVLMEGWVRVPQPGV
HHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
AHPPQIRIPATYMRGGTSKGVFFRLTDLPEAAQVPGAARDALLLRVIGSPDPYEKQIDG
CCCCCEECCHHHHCCCCCCCEEEEEECCCHHHHCCCCHHCEEEEEECCCCCCHHHHHCC
MGGATSSTSKSVIVSRSSRPDHDVDYLFGQVSIDKPFVDWSGNCGNLSAAVGPFAITSGL
CCCCCCCCCCEEEEECCCCCCCCHHHEEEEEEECCCCCCCCCCCCCCCHHHCCHHHHHCC
VDPGRVPQDGVAVVRIWQANIGKTIIAHVPVSNGAVQETGDFELDGVTFPAAEVPLEFLD
CCCCCCCCCCEEEEEEEECCCCCEEEEEECCCCCCCCCCCCEEECCEECCHHHCCHHHHC
PAADEEGAGGSMFPTGNLVDDLQVPGIGTLKATMINAGIPTIFVNAADIGYTGTELQGDI
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHEEECCCCCEEEEECCCCCCCCCCCCCCC
NADPQALARLEAIRAYGALHMGLIGSIDEAAARQHTPKVAFVAAPADYLASSGKPVAAQD
CCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCEEEEECCHHHHHCCCCCCHHHH
IELLVRAVSMGKLHHAMMGTAAVAIGTAAAIPGTLVNLAAGGQARSAVSFGHPSGTLRVG
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHEEEECCCCCCCEEECCCCCCCEEEC
AQAVQEGGDWKVTKALMSRSARVLMEGWVRVPQPGV
HHHHHCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA