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The map label for this gene is tesA [H]

Identifier: 66045308

GI number: 66045308

Start: 2414589

End: 2415206

Strand: Direct

Name: tesA [H]

Synonym: Psyr_2068

Alternate gene names: 66045308

Gene position: 2414589-2415206 (Clockwise)

Preceding gene: 66045305

Following gene: 66045309

Centisome position: 39.62

GC content: 59.06

Gene sequence:

>618_bases
ATGGAGAATCCGATGCGTGCGTTGTTTTTAAGTGCTGGCCTGGGGTTACTGCTGCTGTCACAGGGCGCGTTGGCGGGCAC
GGTGTTGATCGTTGGAGATAGTATCAGTGCGGCTTTCGGGCTGGATACCCGCGTGGGGTGGGTCAGTTTGCTGGAACAGC
GTCTGAGCAAGGAGGGATTCGACGACAAGGTGGTCAATGCGTCGATCAGTGGCGACACCAGTGCAGGCGGCCAGGCGCGT
TTGCCTGCGCTGCTTGCAGAGCATAAGCCGGAGCTGGTTATTCTGGAATTGGGCGGCAACGACGGCCTGCGCGGCCAGCA
GCCTGCTCAATTGCAACAAAACCTTTCATCGATGATCGACCGCTCGCAGGCGGCAGGTGCCAAAGTGCTGTTACTGGGCA
TGCGTATCCCGCCCAACTACGGGCCACGCTACACCAAGGCCTTTGAAGAGGTTTACAGCAACCTGGCCGAGGAAAAAAAG
ATCCCCTTCGTGCCATTCTTTCTGGAAGGTGTAGGCGGGGTGCCCGAGCTGATGCAGGCCGATGGTCTGCATCCGGCAGC
CGCCGCTCAGGGCAAGTTGCTGGAAAATGTCTGGCCGAGCCTGAAACCGCTGCTTTGA

Upstream 100 bases:

>100_bases
CTTGTTTAGTTCCAGGGAGAGTTCGTGCAGGATGGTCAGGTCACCTTCGGTGCTGGGGACTACTTTGCTGAGGCTTTGGG
CACTGAGAATACTTTCACTC

Downstream 100 bases:

>100_bases
GGCTTTCCCGGGCGGGGGCTTTCGGCTAATGTGGCGCCCCCGATCTGGAGCCCCCCATGCCGCGTCCCGCCTGGTCCCTG
TATGCCTATCAAATGCTTGA

Product: arylesterase

Products: NA

Alternate protein names: Lecithinase B; Lysophospholipase L1; Protease I [H]

Number of amino acids: Translated: 205; Mature: 205

Protein sequence:

>205_residues
MENPMRALFLSAGLGLLLLSQGALAGTVLIVGDSISAAFGLDTRVGWVSLLEQRLSKEGFDDKVVNASISGDTSAGGQAR
LPALLAEHKPELVILELGGNDGLRGQQPAQLQQNLSSMIDRSQAAGAKVLLLGMRIPPNYGPRYTKAFEEVYSNLAEEKK
IPFVPFFLEGVGGVPELMQADGLHPAAAAQGKLLENVWPSLKPLL

Sequences:

>Translated_205_residues
MENPMRALFLSAGLGLLLLSQGALAGTVLIVGDSISAAFGLDTRVGWVSLLEQRLSKEGFDDKVVNASISGDTSAGGQAR
LPALLAEHKPELVILELGGNDGLRGQQPAQLQQNLSSMIDRSQAAGAKVLLLGMRIPPNYGPRYTKAFEEVYSNLAEEKK
IPFVPFFLEGVGGVPELMQADGLHPAAAAQGKLLENVWPSLKPLL
>Mature_205_residues
MENPMRALFLSAGLGLLLLSQGALAGTVLIVGDSISAAFGLDTRVGWVSLLEQRLSKEGFDDKVVNASISGDTSAGGQAR
LPALLAEHKPELVILELGGNDGLRGQQPAQLQQNLSSMIDRSQAAGAKVLLLGMRIPPNYGPRYTKAFEEVYSNLAEEKK
IPFVPFFLEGVGGVPELMQADGLHPAAAAQGKLLENVWPSLKPLL

Specific function: Hydrolyzes only long chain acyl thioesters (C12-C18). Specificity similar to chymotrypsin [H]

COG id: COG2755

COG function: function code E; Lysophospholipase L1 and related esterases

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the 'GDSL' lipolytic enzyme family [H]

Homologues:

Organism=Escherichia coli, GI1786702, Length=190, Percent_Identity=46.3157894736842, Blast_Score=169, Evalue=1e-43,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013830
- InterPro:   IPR013831
- InterPro:   IPR001087
- InterPro:   IPR008265 [H]

Pfam domain/function: PF00657 Lipase_GDSL [H]

EC number: =3.1.1.5 [H]

Molecular weight: Translated: 21741; Mature: 21741

Theoretical pI: Translated: 5.01; Mature: 5.01

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MENPMRALFLSAGLGLLLLSQGALAGTVLIVGDSISAAFGLDTRVGWVSLLEQRLSKEGF
CCCHHHHHHHHHCHHHHHHHCCCCEEEEEEECCCCCHHHCCCCHHHHHHHHHHHHHHCCC
DDKVVNASISGDTSAGGQARLPALLAEHKPELVILELGGNDGLRGQQPAQLQQNLSSMID
CCCEEEEECCCCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHH
RSQAAGAKVLLLGMRIPPNYGPRYTKAFEEVYSNLAEEKKIPFVPFFLEGVGGVPELMQA
HHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCHHHHHH
DGLHPAAAAQGKLLENVWPSLKPLL
CCCCHHHHHHHHHHHHHCCHHCCCC
>Mature Secondary Structure
MENPMRALFLSAGLGLLLLSQGALAGTVLIVGDSISAAFGLDTRVGWVSLLEQRLSKEGF
CCCHHHHHHHHHCHHHHHHHCCCCEEEEEEECCCCCHHHCCCCHHHHHHHHHHHHHHCCC
DDKVVNASISGDTSAGGQARLPALLAEHKPELVILELGGNDGLRGQQPAQLQQNLSSMID
CCCEEEEECCCCCCCCCCHHHHHHHHCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHHH
RSQAAGAKVLLLGMRIPPNYGPRYTKAFEEVYSNLAEEKKIPFVPFFLEGVGGVPELMQA
HHHHCCCEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCHHHHHH
DGLHPAAAAQGKLLENVWPSLKPLL
CCCCHHHHHHHHHHHHHCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]