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The map label for this gene is mrcB [H]

Identifier: 66044097

GI number: 66044097

Start: 953935

End: 956256

Strand: Direct

Name: mrcB [H]

Synonym: Psyr_0842

Alternate gene names: 66044097

Gene position: 953935-956256 (Clockwise)

Preceding gene: 66044094

Following gene: 66044098

Centisome position: 15.65

GC content: 60.59

Gene sequence:

>2322_bases
ATGACTCGATCCCGCACCTCTCGTTCTGCAAAAAAACAACCTCCCCGCAGCCTTAACAAGTGGCTGGGCTGGGCGTTGAA
GCTCGGTATTGTCGGCCTGGTTCTGCTCGGCGGCCTGGCCATTTATCTCGATGCAATCGTCCAGGAAAAATTCTCCGGCA
AGCGCTGGACCATCCCGGCCAAGGTCTATGCACGGCCGCTGGAGCTGTTCGTCGGACAGAAGCTGAGCCGCGATGACTTT
CTCATCGAACTCGACGCCCTGGGCTATCGCCGCGAAAGCGTGGCGAATGGTCCCGGCGCCGCTGCGGTGAACGGCAATAC
CGTCGACCTCAATACGCGTGGCTTTCAGTTTTACGAAGGCACTGATGCAGCGCAGCAGGTGCGCGTGCGCTTTTCCGGTG
ACTATGTAGCCGACCTGTCTTCGGGCAATGGTGCCAAGCTTGCGGTGGCACGACTCGAGCCCTTGCTGATCGGCGGCCTG
TACCCGAAGAATCTGGAAGACCGGATCCTGATCAAGCTGGATCAGGCGCCGCCCTATCTGCTCGACGGGCTGGTAGCGGT
CGAGGACCGGGATTTCTATCACCACTTCGGCGTGTCGCCCAAGTCCATTGCCCGGGCGATCTGGGTCAATACCTCGCAGG
GGCAGATGCGCCAGGGCGGCAGTACGCTGACCCAGCAGTTGGTCAAGAACTTCTACCTGACCAATGAACGCAGCCTGACC
CGTAAGCTGACCGAAGCCATGATGGCCGTTCTGCTGGAGATTCATTACAGCAAGCAGGAAATCCTCGAGGCCTATCTCAA
CGAAGTGTTCGTCGGGCAGGACGGCCAGCGCGCCGTTCACGGTTTCGGTCTGGCCAGCCAGTATTTCTTCAGCCAGCCGC
TGTCCGAGCTGAAAATCCATCAGGTGGCGATGCTGGTCGGACTGGTCAAGGGGCCTTCCTTCTACAACCCGCGGCGCAAT
CCGGAGCGTGCTCTGGAGCGTCGTAATCTGGTGCTCGACCTGTTTGAGCAGCAGGGCGTCGCCACTCCCGAAGTAGTCGC
TGCTGCGAAAAAAATGCCACTGGGTGTGACCAAGACCGGGACCCTCGCCGACAGCTCGTTCCCGGCGTTCCTCGATCTGG
TCAAGCGTCAGCTGCGCGAAGACTATCGCGACGAAGACCTGACCGAAGAAGGGCTGCGGATCTTTACCAGCTTCGATCCG
ATCCTGCAGATGAAGTCCCAGGCGGCCATGGACGACACGTTCAAGAAGCTCGCCGGGCGCAAGGGCTCTGATGAAGTGGA
GTCGGCCATGGTCGTCACCAACCCTGAAACCGGCGAAGTGCAGGCGTTGATCGGTGGGCGTGAGGCCGGGTTCTCGGGCT
TCAACCGGGCGATCGACGCGGTGCGTCCGATCGGCTCGCTGGTCAAGCCGGCTATCTACCTGACGGCACTTGAGCGGCCA
AGCCAGTACACCTTGACCAGCTGGATTGCCGACGAACTGTTCCAGGTCAAAGGCGCCGATGGCCAGATATGGAAACCGCA
GAACTACGATCACAAGTCACACGGCAATATCTTCCTGTATCAGGGGCTGGCGCACTCTTACAACCTGTCGACCGCCAAGC
TGGGCCTTGAACTGGGCATTCCAAACGTGTTCAAGACACTGGCCAAACTGGGTGTGACGCGCGAATGGCCGACCTATCCG
TCGATGCTGCTGGGTGCGGGTGGCTTGAGTCCGATGGAAGTGGCCACCATGTATCAGACCATCGCCAGCGGCGGGTTCAA
TACGCCGATGCGTGGTATTCGCAGCGTGCTCACCGCGGAAGGCGAGCCACTCAAGCGTTATCCGTTCCAGATTGAACAGC
GCTTCGATCCGGGTGCCATATACCTGGTGCAGAATGCGATGCAGCGGGTCATGCGCGAAGGTACGGGCAAATCTGTTTAC
AACGTATTGCCGAGCTCGTTGAATCTGGCAGGCAAGACCGGTACCAGTAACGACTCGCGTGACAGCTGGTTTGCCGGGTT
CAGTCAGGACCTGCTGGCGGTGGTGTGGATGGGGCGCGACGACAACGGCAAGACACCGTTTACCGGTGCCACCGGTGCCC
TGCAGGTCTGGACCAGTTTCATGCGCAAGGCCGGCCCGCTGCCGCTGGACATGGCGATGCCGGACAACGTCGTTCAGGCG
TGGGTCGATGCGCAGACCGGTCAGGGGTCGGATGCAAGCTGCCCGAATGCCGTGCAGATGCCGTATATTCGCGGCAGTGA
GCCCCAGCCCGGCGCGACATGCGGCGGCGCTCCGGCACCTGCCACTGAGGTGATGGACTGGGTCAAGGGTTGGTTGAACT
AG

Upstream 100 bases:

>100_bases
TAAGTACACGATGCCGCCATTATGGCCGCTTGGCGCGAGGCTGCAAACCGCTGACTCGGGCGTCTGTCGATCCTCGGTAA
AGTGCGTATAATCCGCGCCC

Downstream 100 bases:

>100_bases
GCAATCGAGGTTTTCAAGTGAATAAATGGTGGATTCCAGCTTTAACGGCACTGGCCTTGCTGAACGGCTGCGCCAGTGTG
GAACGCGGCTCGATTCCGGT

Product: peptidoglycan glycosyltransferase

Products: NA

Alternate protein names: PBP-1b; PBP1b; Murein polymerase; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Peptidoglycan glycosyltransferase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]

Number of amino acids: Translated: 773; Mature: 772

Protein sequence:

>773_residues
MTRSRTSRSAKKQPPRSLNKWLGWALKLGIVGLVLLGGLAIYLDAIVQEKFSGKRWTIPAKVYARPLELFVGQKLSRDDF
LIELDALGYRRESVANGPGAAAVNGNTVDLNTRGFQFYEGTDAAQQVRVRFSGDYVADLSSGNGAKLAVARLEPLLIGGL
YPKNLEDRILIKLDQAPPYLLDGLVAVEDRDFYHHFGVSPKSIARAIWVNTSQGQMRQGGSTLTQQLVKNFYLTNERSLT
RKLTEAMMAVLLEIHYSKQEILEAYLNEVFVGQDGQRAVHGFGLASQYFFSQPLSELKIHQVAMLVGLVKGPSFYNPRRN
PERALERRNLVLDLFEQQGVATPEVVAAAKKMPLGVTKTGTLADSSFPAFLDLVKRQLREDYRDEDLTEEGLRIFTSFDP
ILQMKSQAAMDDTFKKLAGRKGSDEVESAMVVTNPETGEVQALIGGREAGFSGFNRAIDAVRPIGSLVKPAIYLTALERP
SQYTLTSWIADELFQVKGADGQIWKPQNYDHKSHGNIFLYQGLAHSYNLSTAKLGLELGIPNVFKTLAKLGVTREWPTYP
SMLLGAGGLSPMEVATMYQTIASGGFNTPMRGIRSVLTAEGEPLKRYPFQIEQRFDPGAIYLVQNAMQRVMREGTGKSVY
NVLPSSLNLAGKTGTSNDSRDSWFAGFSQDLLAVVWMGRDDNGKTPFTGATGALQVWTSFMRKAGPLPLDMAMPDNVVQA
WVDAQTGQGSDASCPNAVQMPYIRGSEPQPGATCGGAPAPATEVMDWVKGWLN

Sequences:

>Translated_773_residues
MTRSRTSRSAKKQPPRSLNKWLGWALKLGIVGLVLLGGLAIYLDAIVQEKFSGKRWTIPAKVYARPLELFVGQKLSRDDF
LIELDALGYRRESVANGPGAAAVNGNTVDLNTRGFQFYEGTDAAQQVRVRFSGDYVADLSSGNGAKLAVARLEPLLIGGL
YPKNLEDRILIKLDQAPPYLLDGLVAVEDRDFYHHFGVSPKSIARAIWVNTSQGQMRQGGSTLTQQLVKNFYLTNERSLT
RKLTEAMMAVLLEIHYSKQEILEAYLNEVFVGQDGQRAVHGFGLASQYFFSQPLSELKIHQVAMLVGLVKGPSFYNPRRN
PERALERRNLVLDLFEQQGVATPEVVAAAKKMPLGVTKTGTLADSSFPAFLDLVKRQLREDYRDEDLTEEGLRIFTSFDP
ILQMKSQAAMDDTFKKLAGRKGSDEVESAMVVTNPETGEVQALIGGREAGFSGFNRAIDAVRPIGSLVKPAIYLTALERP
SQYTLTSWIADELFQVKGADGQIWKPQNYDHKSHGNIFLYQGLAHSYNLSTAKLGLELGIPNVFKTLAKLGVTREWPTYP
SMLLGAGGLSPMEVATMYQTIASGGFNTPMRGIRSVLTAEGEPLKRYPFQIEQRFDPGAIYLVQNAMQRVMREGTGKSVY
NVLPSSLNLAGKTGTSNDSRDSWFAGFSQDLLAVVWMGRDDNGKTPFTGATGALQVWTSFMRKAGPLPLDMAMPDNVVQA
WVDAQTGQGSDASCPNAVQMPYIRGSEPQPGATCGGAPAPATEVMDWVKGWLN
>Mature_772_residues
TRSRTSRSAKKQPPRSLNKWLGWALKLGIVGLVLLGGLAIYLDAIVQEKFSGKRWTIPAKVYARPLELFVGQKLSRDDFL
IELDALGYRRESVANGPGAAAVNGNTVDLNTRGFQFYEGTDAAQQVRVRFSGDYVADLSSGNGAKLAVARLEPLLIGGLY
PKNLEDRILIKLDQAPPYLLDGLVAVEDRDFYHHFGVSPKSIARAIWVNTSQGQMRQGGSTLTQQLVKNFYLTNERSLTR
KLTEAMMAVLLEIHYSKQEILEAYLNEVFVGQDGQRAVHGFGLASQYFFSQPLSELKIHQVAMLVGLVKGPSFYNPRRNP
ERALERRNLVLDLFEQQGVATPEVVAAAKKMPLGVTKTGTLADSSFPAFLDLVKRQLREDYRDEDLTEEGLRIFTSFDPI
LQMKSQAAMDDTFKKLAGRKGSDEVESAMVVTNPETGEVQALIGGREAGFSGFNRAIDAVRPIGSLVKPAIYLTALERPS
QYTLTSWIADELFQVKGADGQIWKPQNYDHKSHGNIFLYQGLAHSYNLSTAKLGLELGIPNVFKTLAKLGVTREWPTYPS
MLLGAGGLSPMEVATMYQTIASGGFNTPMRGIRSVLTAEGEPLKRYPFQIEQRFDPGAIYLVQNAMQRVMREGTGKSVYN
VLPSSLNLAGKTGTSNDSRDSWFAGFSQDLLAVVWMGRDDNGKTPFTGATGALQVWTSFMRKAGPLPLDMAMPDNVVQAW
VDAQTGQGSDASCPNAVQMPYIRGSEPQPGATCGGAPAPATEVMDWVKGWLN

Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal

COG id: COG0744

COG function: function code M; Membrane carboxypeptidase (penicillin-binding protein)

Gene ontology:

Cell location: Cell inner membrane; Single-pass type II membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786343, Length=733, Percent_Identity=39.9727148703956, Blast_Score=516, Evalue=1e-147,
Organism=Escherichia coli, GI1788867, Length=596, Percent_Identity=27.3489932885906, Blast_Score=163, Evalue=4e-41,
Organism=Escherichia coli, GI87082258, Length=255, Percent_Identity=34.9019607843137, Blast_Score=158, Evalue=1e-39,
Organism=Escherichia coli, GI1789601, Length=142, Percent_Identity=34.5070422535211, Blast_Score=80, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001264
- InterPro:   IPR011813
- InterPro:   IPR001460 [H]

Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]

EC number: =2.4.1.129 [H]

Molecular weight: Translated: 84945; Mature: 84814

Theoretical pI: Translated: 9.05; Mature: 9.05

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTRSRTSRSAKKQPPRSLNKWLGWALKLGIVGLVLLGGLAIYLDAIVQEKFSGKRWTIPA
CCCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCH
KVYARPLELFVGQKLSRDDFLIELDALGYRRESVANGPGAAAVNGNTVDLNTRGFQFYEG
HHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHCCCCCCCEEECCCEEEECCCCEEEECC
TDAAQQVRVRFSGDYVADLSSGNGAKLAVARLEPLLIGGLYPKNLEDRILIKLDQAPPYL
CCHHHHEEEEECCCEEEECCCCCCCEEEHHHHCHHEEECCCCCCCCCEEEEEECCCCCHH
LDGLVAVEDRDFYHHFGVSPKSIARAIWVNTSQGQMRQGGSTLTQQLVKNFYLTNERSLT
HCCEEEEECCCHHHHCCCCHHHHHHHHEEECCCCHHHCCHHHHHHHHHHHHHCCCCHHHH
RKLTEAMMAVLLEIHYSKQEILEAYLNEVFVGQDGQRAVHGFGLASQYFFSQPLSELKIH
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHCCHHHHHHHHCCHHHHHHH
QVAMLVGLVKGPSFYNPRRNPERALERRNLVLDLFEQQGVATPEVVAAAKKMPLGVTKTG
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCC
TLADSSFPAFLDLVKRQLREDYRDEDLTEEGLRIFTSFDPILQMKSQAAMDDTFKKLAGR
CCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHCC
KGSDEVESAMVVTNPETGEVQALIGGREAGFSGFNRAIDAVRPIGSLVKPAIYLTALERP
CCCCHHHCEEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SQYTLTSWIADELFQVKGADGQIWKPQNYDHKSHGNIFLYQGLAHSYNLSTAKLGLELGI
CCHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHCCCC
PNVFKTLAKLGVTREWPTYPSMLLGAGGLSPMEVATMYQTIASGGFNTPMRGIRSVLTAE
CHHHHHHHHHCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCC
GEPLKRYPFQIEQRFDPGAIYLVQNAMQRVMREGTGKSVYNVLPSSLNLAGKTGTSNDSR
CCCHHHCCCHHCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCCCCC
DSWFAGFSQDLLAVVWMGRDDNGKTPFTGATGALQVWTSFMRKAGPLPLDMAMPDNVVQA
CHHHHCHHHCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
WVDAQTGQGSDASCPNAVQMPYIRGSEPQPGATCGGAPAPATEVMDWVKGWLN
HHHCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCC
>Mature Secondary Structure 
TRSRTSRSAKKQPPRSLNKWLGWALKLGIVGLVLLGGLAIYLDAIVQEKFSGKRWTIPA
CCCCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCH
KVYARPLELFVGQKLSRDDFLIELDALGYRRESVANGPGAAAVNGNTVDLNTRGFQFYEG
HHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHCCCCCCCEEECCCEEEECCCCEEEECC
TDAAQQVRVRFSGDYVADLSSGNGAKLAVARLEPLLIGGLYPKNLEDRILIKLDQAPPYL
CCHHHHEEEEECCCEEEECCCCCCCEEEHHHHCHHEEECCCCCCCCCEEEEEECCCCCHH
LDGLVAVEDRDFYHHFGVSPKSIARAIWVNTSQGQMRQGGSTLTQQLVKNFYLTNERSLT
HCCEEEEECCCHHHHCCCCHHHHHHHHEEECCCCHHHCCHHHHHHHHHHHHHCCCCHHHH
RKLTEAMMAVLLEIHYSKQEILEAYLNEVFVGQDGQRAVHGFGLASQYFFSQPLSELKIH
HHHHHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHCCHHHHHHHHCCHHHHHHH
QVAMLVGLVKGPSFYNPRRNPERALERRNLVLDLFEQQGVATPEVVAAAKKMPLGVTKTG
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCCCCC
TLADSSFPAFLDLVKRQLREDYRDEDLTEEGLRIFTSFDPILQMKSQAAMDDTFKKLAGR
CCCCCCCHHHHHHHHHHHHHHCCCCCCCHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHCC
KGSDEVESAMVVTNPETGEVQALIGGREAGFSGFNRAIDAVRPIGSLVKPAIYLTALERP
CCCCHHHCEEEEECCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
SQYTLTSWIADELFQVKGADGQIWKPQNYDHKSHGNIFLYQGLAHSYNLSTAKLGLELGI
CCHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCCCCEEEEECCCCCCCCCHHHHHHCCCC
PNVFKTLAKLGVTREWPTYPSMLLGAGGLSPMEVATMYQTIASGGFNTPMRGIRSVLTAE
CHHHHHHHHHCCCCCCCCCHHHHHCCCCCCHHHHHHHHHHHHCCCCCCHHHHHHHHHHCC
GEPLKRYPFQIEQRFDPGAIYLVQNAMQRVMREGTGKSVYNVLPSSLNLAGKTGTSNDSR
CCCHHHCCCHHCCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCCCCCCCCCCCC
DSWFAGFSQDLLAVVWMGRDDNGKTPFTGATGALQVWTSFMRKAGPLPLDMAMPDNVVQA
CHHHHCHHHCEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCHHHHHH
WVDAQTGQGSDASCPNAVQMPYIRGSEPQPGATCGGAPAPATEVMDWVKGWLN
HHHCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCCCCHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]