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The map label for this gene is livJ [C]

Identifier: 66043864

GI number: 66043864

Start: 685704

End: 686840

Strand: Direct

Name: livJ [C]

Synonym: Psyr_0597

Alternate gene names: 66043864

Gene position: 685704-686840 (Clockwise)

Preceding gene: 66043858

Following gene: 66043865

Centisome position: 11.25

GC content: 59.28

Gene sequence:

>1137_bases
ATGTCGCATACGTTTTACAGGAAAGGTTTTCTGGCAATCGCAGTGGCTACTGCATTGGGCGTGTCTTCGTTTGCACAGGC
GGACGTGAAAATCGGCGTAGCGGGCCCGATGACCGGCGCGAACGCGTCGTTTGGTCAGCAGTACATGAAGGGCGCGCAGG
CAGCAGCCGATGCGATCAATGCGGCAGGTGGTGTCAACGGGGAGAAAATCGTACTGATCGCAGGCGATGACGCTTGTGAG
CCCAAGCAGGCCGTGGCAGTTGCCAACCGCCTGGTGGATCAGGACAAGGTGATTGGCGTGGTCGGGCACTTCTGTTCATC
GTCAACCATCCCGGCGTCTGAGATCTATGACGAAGCAGGCGTGATTGCCATTACGCCCGGCTCGACCAACCCGACAGTGA
CCGAGCGTGGTCTGAAAGATATGTTCCGCATGTGCGGTCGCGATGACCAGCAGGGTGTCGTTGCGGGGGACTACATCGTC
GACGTGCTCAAGGGCAAGAAAGTCGCCGTTATCAATGACAAGGACACCTACGGCAAGGGCCTCGCGGACGCAACGGCCGC
TCAGTTGACCAAGCGTGGCGTCGAGCCGATCCTCGAAGAGGGTCTGACCCGTGGCGAGAAAGATTTCAGTGCGCTGGTCA
CGAAGATTCGTTCGGTAGACGCTGACGTCGTGTACTTCGGCGGTCTGCACCCGGAAGCCGGTCCTTTGGTTCGGCAGATG
CGCGAGCAGGGCCTCAAGGACGTCAAGTTCATGTCCGATGATGGCGTCGTGACCGACGAGCTGGTCACCACTGCAGGTGG
CGCACAGTACGTCGATGGCGTGTACATGACCTTCGGCGCGGACCCGCGTGCATTGCCGGACAGCAAGGCTGTGGTGGATC
AGTTCCGCAAGGCCGGTTACGAGCCTGAAGGTTACACCCTCTACGCCTACGCTTCGGTACAGGCCCTGGCGGCCGGTTTC
AATGGTGCCAAGTCCAACAAGGGTGACGCAGCCGCCACGTGGCTCAAGGCCAACCCGGTCAAGACCGTCATGGGTGAAAA
AGCCTGGGATGCCAAGGGTGACCTGAAAGTCTCCGACTATGTGGTTTATCAGTGGGACGCCGCCGGCAAATACAAGCAAC
TCGAAAAACAGAAGTGA

Upstream 100 bases:

>100_bases
CTCAACCAAAAAGCGATGGCACGCGCCTTGCTCTGAGCAACTCTGTATCTGTTCGATCATGAAAGTGCCAACTAAAAAAA
TACCCTCAGGAGCACCACTT

Downstream 100 bases:

>100_bases
GATAGACCTCGCGTCAGTCCTGCGCGTCAGGTGCTGACCCGGTGATGGGCTCGCCCATGAGCGAGCCTGCATCTCTTTTA
TCGTTTTTGGACCTGTCGGT

Product: extracellular ligand-binding receptor

Products: ADP; phosphate; L-leucine [Cytoplasm]; ADP; L-valine [Cytoplasm]; L-iso-leucine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 378; Mature: 377

Protein sequence:

>378_residues
MSHTFYRKGFLAIAVATALGVSSFAQADVKIGVAGPMTGANASFGQQYMKGAQAAADAINAAGGVNGEKIVLIAGDDACE
PKQAVAVANRLVDQDKVIGVVGHFCSSSTIPASEIYDEAGVIAITPGSTNPTVTERGLKDMFRMCGRDDQQGVVAGDYIV
DVLKGKKVAVINDKDTYGKGLADATAAQLTKRGVEPILEEGLTRGEKDFSALVTKIRSVDADVVYFGGLHPEAGPLVRQM
REQGLKDVKFMSDDGVVTDELVTTAGGAQYVDGVYMTFGADPRALPDSKAVVDQFRKAGYEPEGYTLYAYASVQALAAGF
NGAKSNKGDAAATWLKANPVKTVMGEKAWDAKGDLKVSDYVVYQWDAAGKYKQLEKQK

Sequences:

>Translated_378_residues
MSHTFYRKGFLAIAVATALGVSSFAQADVKIGVAGPMTGANASFGQQYMKGAQAAADAINAAGGVNGEKIVLIAGDDACE
PKQAVAVANRLVDQDKVIGVVGHFCSSSTIPASEIYDEAGVIAITPGSTNPTVTERGLKDMFRMCGRDDQQGVVAGDYIV
DVLKGKKVAVINDKDTYGKGLADATAAQLTKRGVEPILEEGLTRGEKDFSALVTKIRSVDADVVYFGGLHPEAGPLVRQM
REQGLKDVKFMSDDGVVTDELVTTAGGAQYVDGVYMTFGADPRALPDSKAVVDQFRKAGYEPEGYTLYAYASVQALAAGF
NGAKSNKGDAAATWLKANPVKTVMGEKAWDAKGDLKVSDYVVYQWDAAGKYKQLEKQK
>Mature_377_residues
SHTFYRKGFLAIAVATALGVSSFAQADVKIGVAGPMTGANASFGQQYMKGAQAAADAINAAGGVNGEKIVLIAGDDACEP
KQAVAVANRLVDQDKVIGVVGHFCSSSTIPASEIYDEAGVIAITPGSTNPTVTERGLKDMFRMCGRDDQQGVVAGDYIVD
VLKGKKVAVINDKDTYGKGLADATAAQLTKRGVEPILEEGLTRGEKDFSALVTKIRSVDADVVYFGGLHPEAGPLVRQMR
EQGLKDVKFMSDDGVVTDELVTTAGGAQYVDGVYMTFGADPRALPDSKAVVDQFRKAGYEPEGYTLYAYASVQALAAGFN
GAKSNKGDAAATWLKANPVKTVMGEKAWDAKGDLKVSDYVVYQWDAAGKYKQLEKQK

Specific function: Component of an amino-acid transport system (Potential) [H]

COG id: COG0683

COG function: function code E; ABC-type branched-chain amino acid transport systems, periplasmic component

Gene ontology:

Cell location: Periplasmic Protein [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the leucine-binding protein family [H]

Homologues:

Organism=Escherichia coli, GI48994941, Length=364, Percent_Identity=42.032967032967, Blast_Score=272, Evalue=3e-74,
Organism=Escherichia coli, GI1789867, Length=365, Percent_Identity=41.6438356164384, Blast_Score=267, Evalue=8e-73,

Paralogues:

None

Copy number: 10140 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 8822 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal med

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001828
- InterPro:   IPR000709 [H]

Pfam domain/function: PF01094 ANF_receptor [H]

EC number: NA

Molecular weight: Translated: 39920; Mature: 39789

Theoretical pI: Translated: 5.30; Mature: 5.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSHTFYRKGFLAIAVATALGVSSFAQADVKIGVAGPMTGANASFGQQYMKGAQAAADAIN
CCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHH
AAGGVNGEKIVLIAGDDACEPKQAVAVANRLVDQDKVIGVVGHFCSSSTIPASEIYDEAG
HCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCC
VIAITPGSTNPTVTERGLKDMFRMCGRDDQQGVVAGDYIVDVLKGKKVAVINDKDTYGKG
EEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHCCCEEEEECCCCCCCCC
LADATAAQLTKRGVEPILEEGLTRGEKDFSALVTKIRSVDADVVYFGGLHPEAGPLVRQM
CHHHHHHHHHHHCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHH
REQGLKDVKFMSDDGVVTDELVTTAGGAQYVDGVYMTFGADPRALPDSKAVVDQFRKAGY
HHCCCHHEEEECCCCCCHHHHHHCCCCHHHHCCEEEEECCCCCCCCCHHHHHHHHHHCCC
EPEGYTLYAYASVQALAAGFNGAKSNKGDAAATWLKANPVKTVMGEKAWDAKGDLKVSDY
CCCCEEEEEEHHHHHHHHCCCCCCCCCCCHHEEEEECCCHHHHHCCCCCCCCCCEEEEEE
VVYQWDAAGKYKQLEKQK
EEEEECCCCCHHHHHCCC
>Mature Secondary Structure 
SHTFYRKGFLAIAVATALGVSSFAQADVKIGVAGPMTGANASFGQQYMKGAQAAADAIN
CCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHH
AAGGVNGEKIVLIAGDDACEPKQAVAVANRLVDQDKVIGVVGHFCSSSTIPASEIYDEAG
HCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCC
VIAITPGSTNPTVTERGLKDMFRMCGRDDQQGVVAGDYIVDVLKGKKVAVINDKDTYGKG
EEEECCCCCCCCHHHHHHHHHHHHHCCCCCCCEEEHHHHHHHHCCCEEEEECCCCCCCCC
LADATAAQLTKRGVEPILEEGLTRGEKDFSALVTKIRSVDADVVYFGGLHPEAGPLVRQM
CHHHHHHHHHHHCHHHHHHHHCCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCHHHHHH
REQGLKDVKFMSDDGVVTDELVTTAGGAQYVDGVYMTFGADPRALPDSKAVVDQFRKAGY
HHCCCHHEEEECCCCCCHHHHHHCCCCHHHHCCEEEEECCCCCCCCCHHHHHHHHHHCCC
EPEGYTLYAYASVQALAAGFNGAKSNKGDAAATWLKANPVKTVMGEKAWDAKGDLKVSDY
CCCCEEEEEEHHHHHHHHCCCCCCCCCCCHHEEEEECCCHHHHHCCCCCCCCCCEEEEEE
VVYQWDAAGKYKQLEKQK
EEEEECCCCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; L-leucine [Periplasm]; H2O; ATP; L-valine [Periplasm]; L-iso-leucine [Periplasm] [C]

Specific reaction: ATP + L-leucine [Periplasm] + H2O = ADP + phosphate + L-leucine [Cytoplasm] ATP + L-valine [Periplasm] + H2O = ADP + phosphate + L-valine [Cytoplasm] ATP + L-iso-leucine [Periplasm] + H2O = ADP + phosphate + L-iso-leucine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA