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The map label for this gene is rlmB [H]

Identifier: 66043847

GI number: 66043847

Start: 662568

End: 663323

Strand: Direct

Name: rlmB [H]

Synonym: Psyr_0580

Alternate gene names: 66043847

Gene position: 662568-663323 (Clockwise)

Preceding gene: 66043846

Following gene: 66043848

Centisome position: 10.87

GC content: 64.15

Gene sequence:

>756_bases
ATGAGTCAGCTGGAAAAAATCTACGGCGTTCACGCCGTAGAAGCGTTACTTCGTCATCATCCGAAACGCGTCAAGCACAT
CTGGCTGGCCGAGGGTCGTAACGACCCTCGCGTCCAGACGCTGGTGGCGCTGGCAGCCGAGAATCGCGTGACCATCGGGC
AGGCCGAGCGCCGCGAAATGGATGCCTGGGTCGAGGGCGTGCATCAGGGCGTTGTTGCCGACGTGAGCCCGAGTCAGGTC
TGGGGCGAGGCCATGCTCGACGAACTGCTGGACCGCAGTGAAGGCTCGCCGCTGCTGTTGGTGCTGGACGGCGTGACCGA
TCCGCACAACCTCGGCGCTTGCCTGCGTACTGCTGATGCAGCAGGTGCGCTGGCGGTCATCGTACCCAAGGACAAATCCG
CAACCCTGACGCCGGCTGTGCGCAAGGTCGCCTGCGGTGCTGCCGAGGTCATCCCGCTCGTGGCCGTGACCAACCTGGCG
CGTACGCTGGAAAAACTGCAGCAGCGCGGATTGTGGGTGGTGGGGACTGCGGGCGAGGCCGAGGTCGAGCTGTATCAGCA
GGACCTGACCGGGCCGACCATCATTATCATGGGCGCCGAAGGCAAGGGCATGCGTCGCCTGACCCGTGAGCATTGTGATT
ATCTGGTGCGCCTGCCGATGGCGGGCAGTGTCAGCAGTCTCAATGTGTCGGTCGCGACCGGCGTGTGTCTGTTTGAAGCG
CTGCGCCAGCGATCCGCCAAGCGGAAGCCGGCTTAG

Upstream 100 bases:

>100_bases
CATCGGGCAAGCCGGCCAGCAAACACCGTAAAGGCTCGTCAAAAACGGGCTCCGCGCCAGCTGCGAAAAGCAGTGGCGCT
CGTAAACCCAAGGCCAAGTC

Downstream 100 bases:

>100_bases
TAGCAGCTGCAAGCTGCAAGCCTCAAGCTACAAGCTACAAGCGGACTGCTTCTACTTGTAGCTTTCCACTTGCAGCTTGC
AGCTGCTTCCCTGTTTGTAG

Product: RNA methyltransferase TrmH, group 3

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 251; Mature: 250

Protein sequence:

>251_residues
MSQLEKIYGVHAVEALLRHHPKRVKHIWLAEGRNDPRVQTLVALAAENRVTIGQAERREMDAWVEGVHQGVVADVSPSQV
WGEAMLDELLDRSEGSPLLLVLDGVTDPHNLGACLRTADAAGALAVIVPKDKSATLTPAVRKVACGAAEVIPLVAVTNLA
RTLEKLQQRGLWVVGTAGEAEVELYQQDLTGPTIIIMGAEGKGMRRLTREHCDYLVRLPMAGSVSSLNVSVATGVCLFEA
LRQRSAKRKPA

Sequences:

>Translated_251_residues
MSQLEKIYGVHAVEALLRHHPKRVKHIWLAEGRNDPRVQTLVALAAENRVTIGQAERREMDAWVEGVHQGVVADVSPSQV
WGEAMLDELLDRSEGSPLLLVLDGVTDPHNLGACLRTADAAGALAVIVPKDKSATLTPAVRKVACGAAEVIPLVAVTNLA
RTLEKLQQRGLWVVGTAGEAEVELYQQDLTGPTIIIMGAEGKGMRRLTREHCDYLVRLPMAGSVSSLNVSVATGVCLFEA
LRQRSAKRKPA
>Mature_250_residues
SQLEKIYGVHAVEALLRHHPKRVKHIWLAEGRNDPRVQTLVALAAENRVTIGQAERREMDAWVEGVHQGVVADVSPSQVW
GEAMLDELLDRSEGSPLLLVLDGVTDPHNLGACLRTADAAGALAVIVPKDKSATLTPAVRKVACGAAEVIPLVAVTNLAR
TLEKLQQRGLWVVGTAGEAEVELYQQDLTGPTIIIMGAEGKGMRRLTREHCDYLVRLPMAGSVSSLNVSVATGVCLFEAL
RQRSAKRKPA

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=269, Percent_Identity=30.8550185873606, Blast_Score=98, Evalue=6e-21,
Organism=Escherichia coli, GI1790623, Length=242, Percent_Identity=58.2644628099173, Blast_Score=284, Evalue=5e-78,
Organism=Escherichia coli, GI1788935, Length=241, Percent_Identity=26.5560165975104, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI1790083, Length=164, Percent_Identity=32.3170731707317, Blast_Score=74, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17555908, Length=254, Percent_Identity=27.5590551181102, Blast_Score=73, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6324775, Length=287, Percent_Identity=26.8292682926829, Blast_Score=90, Evalue=4e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 27111; Mature: 26980

Theoretical pI: Translated: 7.59; Mature: 7.59

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQLEKIYGVHAVEALLRHHPKRVKHIWLAEGRNDPRVQTLVALAAENRVTIGQAERREM
CCHHHHHHHHHHHHHHHHHCHHHHHEEEEECCCCCCHHHHHHHHHHCCCEEECCHHHHHH
DAWVEGVHQGVVADVSPSQVWGEAMLDELLDRSEGSPLLLVLDGVTDPHNLGACLRTADA
HHHHHHHHCCCEECCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHCCC
AGALAVIVPKDKSATLTPAVRKVACGAAEVIPLVAVTNLARTLEKLQQRGLWVVGTAGEA
CCEEEEEEECCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCH
EVELYQQDLTGPTIIIMGAEGKGMRRLTREHCDYLVRLPMAGSVSSLNVSVATGVCLFEA
HHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHEEECCCCCCCCCCEEHHHHHHHHHHH
LRQRSAKRKPA
HHHHHCCCCCC
>Mature Secondary Structure 
SQLEKIYGVHAVEALLRHHPKRVKHIWLAEGRNDPRVQTLVALAAENRVTIGQAERREM
CHHHHHHHHHHHHHHHHHCHHHHHEEEEECCCCCCHHHHHHHHHHCCCEEECCHHHHHH
DAWVEGVHQGVVADVSPSQVWGEAMLDELLDRSEGSPLLLVLDGVTDPHNLGACLRTADA
HHHHHHHHCCCEECCCHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCHHHHHHHHHCCC
AGALAVIVPKDKSATLTPAVRKVACGAAEVIPLVAVTNLARTLEKLQQRGLWVVGTAGEA
CCEEEEEEECCCCCCCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCH
EVELYQQDLTGPTIIIMGAEGKGMRRLTREHCDYLVRLPMAGSVSSLNVSVATGVCLFEA
HHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHEEECCCCCCCCCCEEHHHHHHHHHHH
LRQRSAKRKPA
HHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12928499 [H]