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The map label for this gene is miaA [H]

Identifier: 66043838

GI number: 66043838

Start: 649554

End: 650525

Strand: Direct

Name: miaA [H]

Synonym: Psyr_0571

Alternate gene names: 66043838

Gene position: 649554-650525 (Clockwise)

Preceding gene: 66043837

Following gene: 66043839

Centisome position: 10.66

GC content: 58.95

Gene sequence:

>972_bases
ATGAACGCTTTACCTCCGGCCATCTTTCTCATGGGGCCTACCGCTGCAGGCAAGACCGACCTTGCGATAGAACTGACCAA
AGTGCTGCCCTGCGAGTTGATCAGCGTCGATTCTGCGCTGGTCTATCGCGGCATGGACATCGGCACCGCCAAGCCGTCGA
AGACGCAGCTGGCTGAACATCCTCACCGTCTGATCGACATTCTCGACCCGGCGCAAAGCTACTCCGCAGCCGATTTTCGC
AGTGACGCTCTGGCGGCCATGGCGGAAATTACCGCACGCGGAAATATTCCTTTGCTGGTCGGCGGCACTATGTTGTATTT
CAAGGCTCTATTGGACGGGCTGGCGGATATGCCGGCGGCCGATGCACAGGTCAGGGCGCAGCTTGAGGCGGATGCACAGG
CCTTCGGCTGGCAGGCATTGCACGATCAGTTGGCGGTGGTCGATCCCGTTTCGGCGGCACGTATTCACCCCAACGATCCG
CAACGGCTGATCAGGGCGCTTGAGGTGTATCGCGTCAGCGGAATGAGCATGACGGCACATCGCGAGCAACAAACTGCGCA
AAGTACCGAAGCAGCCGCATCAGGGCGCCAGCAATTGCCCTATACTGTCGCGAACCTTGCCATTGCTCCGGCTGATCGCA
AGGTGCTGCACCAACGCATCGCGCTACGTTTCGAGCAAATGCTGGATCAGGGGTTTCTGGACGAAGTACTGGCATTGCGC
TCAAGAGGTGACCTGCATTCGGGGTTGCCATCGATAAGAGCCGTCGGTTATCGCCAGGTCTGGGATCACCTGGATGGGAA
ACTGACACGGGATGAAATGCAGGAGCGCGGCATCATTGCGACGCGCCAGCTGGCCAAAAGGCAGTTCACCTGGCTACGTA
GCTGGGACGACCTGCACTGGCTGGACAGCCTGGCCAGCGACAATCTGTCGCGCGCCTTGAAATACCTGGGATCGGTCTCC
ATATTGAGCTGA

Upstream 100 bases:

>100_bases
GGAAAACACCGAGCGCAGCGGGCAATGCAACCACGGTCGTCCTACCTGGACCCAGATGGGTCTCAATGACCTGGATAAAT
TATTCCTGCGCGGTCAATGA

Downstream 100 bases:

>100_bases
GTCCCTGCAATAGCCGTCTATCCTTGGGGGGTGGTCGGTCAAGCAAATCGAATTCGATTTATTATTTTATTGATCCTTAA
AGGAGTGCGGCACATGTCAA

Product: tRNA delta(2)-isopentenylpyrophosphate transferase

Products: NA

Alternate protein names: Dimethylallyl diphosphate:tRNA dimethylallyltransferase; DMAPP:tRNA dimethylallyltransferase; DMATase; Isopentenyl-diphosphate:tRNA isopentenyltransferase; IPP transferase; IPPT; IPTase [H]

Number of amino acids: Translated: 323; Mature: 323

Protein sequence:

>323_residues
MNALPPAIFLMGPTAAGKTDLAIELTKVLPCELISVDSALVYRGMDIGTAKPSKTQLAEHPHRLIDILDPAQSYSAADFR
SDALAAMAEITARGNIPLLVGGTMLYFKALLDGLADMPAADAQVRAQLEADAQAFGWQALHDQLAVVDPVSAARIHPNDP
QRLIRALEVYRVSGMSMTAHREQQTAQSTEAAASGRQQLPYTVANLAIAPADRKVLHQRIALRFEQMLDQGFLDEVLALR
SRGDLHSGLPSIRAVGYRQVWDHLDGKLTRDEMQERGIIATRQLAKRQFTWLRSWDDLHWLDSLASDNLSRALKYLGSVS
ILS

Sequences:

>Translated_323_residues
MNALPPAIFLMGPTAAGKTDLAIELTKVLPCELISVDSALVYRGMDIGTAKPSKTQLAEHPHRLIDILDPAQSYSAADFR
SDALAAMAEITARGNIPLLVGGTMLYFKALLDGLADMPAADAQVRAQLEADAQAFGWQALHDQLAVVDPVSAARIHPNDP
QRLIRALEVYRVSGMSMTAHREQQTAQSTEAAASGRQQLPYTVANLAIAPADRKVLHQRIALRFEQMLDQGFLDEVLALR
SRGDLHSGLPSIRAVGYRQVWDHLDGKLTRDEMQERGIIATRQLAKRQFTWLRSWDDLHWLDSLASDNLSRALKYLGSVS
ILS
>Mature_323_residues
MNALPPAIFLMGPTAAGKTDLAIELTKVLPCELISVDSALVYRGMDIGTAKPSKTQLAEHPHRLIDILDPAQSYSAADFR
SDALAAMAEITARGNIPLLVGGTMLYFKALLDGLADMPAADAQVRAQLEADAQAFGWQALHDQLAVVDPVSAARIHPNDP
QRLIRALEVYRVSGMSMTAHREQQTAQSTEAAASGRQQLPYTVANLAIAPADRKVLHQRIALRFEQMLDQGFLDEVLALR
SRGDLHSGLPSIRAVGYRQVWDHLDGKLTRDEMQERGIIATRQLAKRQFTWLRSWDDLHWLDSLASDNLSRALKYLGSVS
ILS

Specific function: Catalyzes the transfer of a dimethylallyl group onto the adenine at position 37 in tRNAs that read codons beginning with uridine, leading to the formation of N6-(dimethylallyl)adenosine (i(6)A) [H]

COG id: COG0324

COG function: function code J; tRNA delta(2)-isopentenylpyrophosphate transferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the IPP transferase family [H]

Homologues:

Organism=Homo sapiens, GI31581534, Length=320, Percent_Identity=28.4375, Blast_Score=126, Evalue=2e-29,
Organism=Escherichia coli, GI1790613, Length=301, Percent_Identity=64.7840531561462, Blast_Score=390, Evalue=1e-110,
Organism=Caenorhabditis elegans, GI25144712, Length=304, Percent_Identity=28.6184210526316, Blast_Score=117, Evalue=6e-27,
Organism=Saccharomyces cerevisiae, GI6324848, Length=306, Percent_Identity=26.4705882352941, Blast_Score=116, Evalue=5e-27,
Organism=Drosophila melanogaster, GI24649830, Length=287, Percent_Identity=32.404181184669, Blast_Score=131, Evalue=5e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002627
- InterPro:   IPR018022 [H]

Pfam domain/function: PF01715 IPPT [H]

EC number: =2.5.1.75 [H]

Molecular weight: Translated: 35493; Mature: 35493

Theoretical pI: Translated: 6.61; Mature: 6.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNALPPAIFLMGPTAAGKTDLAIELTKVLPCELISVDSALVYRGMDIGTAKPSKTQLAEH
CCCCCCEEEEECCCCCCCCCCEEEHHHHCCHHHHHHCHHHHHCCCCCCCCCCCHHHHHHC
PHRLIDILDPAQSYSAADFRSDALAAMAEITARGNIPLLVGGTMLYFKALLDGLADMPAA
HHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHHCCCCC
DAQVRAQLEADAQAFGWQALHDQLAVVDPVSAARIHPNDPQRLIRALEVYRVSGMSMTAH
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCHHHHHHHHHHHHHCCCCHHHH
REQQTAQSTEAAASGRQQLPYTVANLAIAPADRKVLHQRIALRFEQMLDQGFLDEVLALR
HHHHHHHHHHHHHCCCCCCCCHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRGDLHSGLPSIRAVGYRQVWDHLDGKLTRDEMQERGIIATRQLAKRQFTWLRSWDDLHW
HCCCHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHH
LDSLASDNLSRALKYLGSVSILS
HHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MNALPPAIFLMGPTAAGKTDLAIELTKVLPCELISVDSALVYRGMDIGTAKPSKTQLAEH
CCCCCCEEEEECCCCCCCCCCEEEHHHHCCHHHHHHCHHHHHCCCCCCCCCCCHHHHHHC
PHRLIDILDPAQSYSAADFRSDALAAMAEITARGNIPLLVGGTMLYFKALLDGLADMPAA
HHHHHHHHCCHHHCCHHHHHHHHHHHHHHHHHCCCCCEEECHHHHHHHHHHHHHHCCCCC
DAQVRAQLEADAQAFGWQALHDQLAVVDPVSAARIHPNDPQRLIRALEVYRVSGMSMTAH
CHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCHHHHHHHHHHHHHCCCCHHHH
REQQTAQSTEAAASGRQQLPYTVANLAIAPADRKVLHQRIALRFEQMLDQGFLDEVLALR
HHHHHHHHHHHHHCCCCCCCCHHHHEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SRGDLHSGLPSIRAVGYRQVWDHLDGKLTRDEMQERGIIATRQLAKRQFTWLRSWDDLHW
HCCCHHHCCHHHHHHHHHHHHHHHCCCCCHHHHHHCCCHHHHHHHHHHHHHHHCCCHHHH
LDSLASDNLSRALKYLGSVSILS
HHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA