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The map label for this gene is nudF [H]

Identifier: 66043814

GI number: 66043814

Start: 619676

End: 620323

Strand: Direct

Name: nudF [H]

Synonym: Psyr_0547

Alternate gene names: 66043814

Gene position: 619676-620323 (Clockwise)

Preceding gene: 66043812

Following gene: 66043815

Centisome position: 10.17

GC content: 56.79

Gene sequence:

>648_bases
ATGTCAAACACGGCGGCCAGCGAGAAACCCATGACCCAGACCACACGATCTGCACCAAGCGCTTTTGAAACCATAAAGCG
TGAAAACTGCTTCAAGGGCTTCTATAAGCTGGACAAGCTGCATGTGCGCCACGAATTGTTCGCCGGCGGGATGAGCAAGG
AAATCAGCCGCGAACTGTTCGTTCGCCACGACGCCGTCTGCGTGCTGCCTTATGACGCCAAACGTGACGAAGTGGTGCTC
ATCGAGCAGTTTCGTGTAGGCGCCATGAAAAAGACCAACAACCCTTGGCTGGTCGAACTGGTCGCAGGTCTGATCGACAA
GGACGAGCAGCCGGAAGAAGTTGCTCATCGCGAGGCGGAGGAGGAAGCTGGGCTGAAGTTCGAAGCGCTGTGGCCGATCA
CCAAATACTTCCCCTCGCCGGGTGGCAGCGACGAGTTCGTTCACCTGTTTCTGGGGCGTTGCAGCAGTGAAGGGGCGGGC
GGCTTGCACGGGCTGGAGGCCGAAGGTGAAGACATCCGGGTAACAGTCTGGTCATTCGATGACGCATTGCAGGCGATGAA
AGACGGAACAATCAAGAACGCATCGACGATCATCGCCTTGCAATGGCTGGCGCTGAATCGTGCCGAGATAAGGGGTTTAT
GGTCGTGA

Upstream 100 bases:

>100_bases
GTTCGATCATGGCAGATTAGAATTGTTTTAAGTCCCGGCCGTCTACCCTGCATCTTCAAGGGGGCTGCATCTGACTGCCG
ATGCGCGTTAGGATGGCCCG

Downstream 100 bases:

>100_bases
ATCTATTGCGCGAGCGCTACCGTGTCGATCTTGCCGGACTGCAGGCTGCGTGCGAGGCCAACTACGCGCGCCTGATGCGC
CTGTTGCCCGACATGCGCAA

Product: hypothetical protein

Products: NA

Alternate protein names: ADP-ribose diphosphatase; ADP-ribose phosphohydrolase; ASPPase; Adenosine diphosphoribose pyrophosphatase; ADPR-PPase [H]

Number of amino acids: Translated: 215; Mature: 214

Protein sequence:

>215_residues
MSNTAASEKPMTQTTRSAPSAFETIKRENCFKGFYKLDKLHVRHELFAGGMSKEISRELFVRHDAVCVLPYDAKRDEVVL
IEQFRVGAMKKTNNPWLVELVAGLIDKDEQPEEVAHREAEEEAGLKFEALWPITKYFPSPGGSDEFVHLFLGRCSSEGAG
GLHGLEAEGEDIRVTVWSFDDALQAMKDGTIKNASTIIALQWLALNRAEIRGLWS

Sequences:

>Translated_215_residues
MSNTAASEKPMTQTTRSAPSAFETIKRENCFKGFYKLDKLHVRHELFAGGMSKEISRELFVRHDAVCVLPYDAKRDEVVL
IEQFRVGAMKKTNNPWLVELVAGLIDKDEQPEEVAHREAEEEAGLKFEALWPITKYFPSPGGSDEFVHLFLGRCSSEGAG
GLHGLEAEGEDIRVTVWSFDDALQAMKDGTIKNASTIIALQWLALNRAEIRGLWS
>Mature_214_residues
SNTAASEKPMTQTTRSAPSAFETIKRENCFKGFYKLDKLHVRHELFAGGMSKEISRELFVRHDAVCVLPYDAKRDEVVLI
EQFRVGAMKKTNNPWLVELVAGLIDKDEQPEEVAHREAEEEAGLKFEALWPITKYFPSPGGSDEFVHLFLGRCSSEGAGG
LHGLEAEGEDIRVTVWSFDDALQAMKDGTIKNASTIIALQWLALNRAEIRGLWS

Specific function: Acts on ADP-mannose and ADP-glucose as well as ADP- ribose. Prevents glycogen biosynthesis. The reaction catalyzed by this enzyme is a limiting step of the gluconeogenic process [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 nudix hydrolase domain [H]

Homologues:

Organism=Escherichia coli, GI1789412, Length=207, Percent_Identity=43.9613526570048, Blast_Score=181, Evalue=2e-47,
Organism=Escherichia coli, GI1788810, Length=151, Percent_Identity=36.4238410596026, Blast_Score=88, Evalue=5e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004385
- InterPro:   IPR020084
- InterPro:   IPR000086
- InterPro:   IPR015797 [H]

Pfam domain/function: PF00293 NUDIX [H]

EC number: =3.6.1.13 [H]

Molecular weight: Translated: 24085; Mature: 23953

Theoretical pI: Translated: 5.19; Mature: 5.19

Prosite motif: PS00893 NUDIX

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSNTAASEKPMTQTTRSAPSAFETIKRENCFKGFYKLDKLHVRHELFAGGMSKEISRELF
CCCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
VRHDAVCVLPYDAKRDEVVLIEQFRVGAMKKTNNPWLVELVAGLIDKDEQPEEVAHREAE
HHCCCEEEECCCCCCCCEEEEECCCCCCEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHH
EEAGLKFEALWPITKYFPSPGGSDEFVHLFLGRCSSEGAGGLHGLEAEGEDIRVTVWSFD
HHHCCEEEEECCHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCH
DALQAMKDGTIKNASTIIALQWLALNRAEIRGLWS
HHHHHHHCCCCCCCHHEEEEHHHHCCHHHHCCCCC
>Mature Secondary Structure 
SNTAASEKPMTQTTRSAPSAFETIKRENCFKGFYKLDKLHVRHELFAGGMSKEISRELF
CCCCCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
VRHDAVCVLPYDAKRDEVVLIEQFRVGAMKKTNNPWLVELVAGLIDKDEQPEEVAHREAE
HHCCCEEEECCCCCCCCEEEEECCCCCCEECCCCCHHHHHHHHHHCCCCCHHHHHHHHHH
EEAGLKFEALWPITKYFPSPGGSDEFVHLFLGRCSSEGAGGLHGLEAEGEDIRVTVWSFD
HHHCCEEEEECCHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECCH
DALQAMKDGTIKNASTIIALQWLALNRAEIRGLWS
HHHHHHHCCCCCCCHHEEEEHHHHCCHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]