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The map label for this gene is thiD1

Identifier: 62390346

GI number: 62390346

Start: 1541284

End: 1543575

Strand: Reverse

Name: thiD1

Synonym: cg1654

Alternate gene names: 62390346

Gene position: 1543575-1541284 (Counterclockwise)

Preceding gene: 62390347

Following gene: 62390345

Centisome position: 47.02

GC content: 57.5

Gene sequence:

>2292_bases
GTGACTGATTTTTCTTTGTATCTGGTCACCGATCCCGTTTTGGGTGGCGGGCCAGAAAAAGTAGCTGGAATTGTTGACAG
CGCAATTTCCGGCGGAGTTTCTGTGGTGCAGCTGCGCGATAAGAACTCAGGCGTGGAAGATGTTCGTGCGGCAGCAAAGG
AGCTGAAAGAACTCTGCGATGCTCGCGGGGTGGCGCTTGTTGTCAACGATTACTTAGATATCGCCGTTGAGCTGGGTCTT
CACCTGCACATTGGTCAAGGCGATACACCTTATACGCAAGCACGGGAGCTGCTTCCAGCTCATCTTGAATTGGGTTTGAG
CATTGAAAACCTGGATCAATTGCATGCTGTGATCGCGCAGTGCGCCGAGACTGGTGTGGCATTGCCCGATGTGATTGGCA
TTGGTCCGGTGGCCTCTACTGCGACCAAACCAGATGCGGCACCCGCATTGGGTGTGGAGGGCATCGCTGAGATCGCCGCT
GTAGCTCAAGACCACGGCATCGCATCAGTAGCTATTGGAGGCGTTGGTCTACGCAACGCGGCCGAACTCGCTGCTACGCC
CATCGACGGTCTGTGCGTGGTCTCTGAAATCATGACCGCCGCCAATCCAGCAGCTGCGGCAACTCGCCTGCGGACTGCTT
TTCAACCTACTTTCTCGCCTGAAACTCAAACTGAACTCTCTCAAACAGAACTCCAAGGAGCCTTCGTGAATTCGCCTTCT
GCCCCACGTGTGTTGTCTATTGCAGGCACTGATCCCACAGGTGGTGCAGGTATTCAGGCTGATCTGAAGTCCATTGCAGC
AGGTGGCGGCTACGGCATGTGCGTTGTGACCTCGCTGGTCGCGCAAAACACCCACGGCGTCAACACGATCCACACCCCAC
CCTTGACCTTTTTGGAAGAACAGCTGGAAGCGGTCTTTTCCGATGTCACCGTCGATGCCATCAAGCTCGGCATGTTGGGC
TCTGCCGACACCGTCGATCTGGTGGCTTCATGGCTTGGTTCCCACGAGCACGGTCCCGTGGTGCTTGATCCCGTCATGAT
CGCCACCAGCGGTGATCGCCTACTGGATGCGAGCGCTGAAGAATCGCTGCGCCGCCTGGCCGTGCACGTCGATGTGGTCA
CCCCGAATATCCCCGAACTTGCCGTGTTGTGCGACAGTGCTCCTGCCATCACCATGGATGAGGCCATTGCTCAGGCTCAG
GGATTTGCGCGGACTCATGACACCATCGTCATTGTCAAGGGTGGACATCTGACTGGCGCGCTTGCTGATAACGCTGTCGT
GCGCCCCGACGGCTCGGTGTTCCAGGTGGAAAACCTGCGTGTCAACACCACCAACTCCCATGGCACAGGCTGTTCGCTCT
CTGCGTCACTTGCCACCAAGATCGCCGCCGGCGAAAGCGTGGAAAAAGCCCTCGAGTGGTCCACGCGTTGGCTCAACGAG
GCGCTCCGCCACGCCGATCACTTGGCTGTAGGTACTGGAAACGGCCCTGTCGACCACGGTCACCTGGCACGACGCATGAC
CCACGCGGCGGAAACCACTCCCTGGGCACACCTTCGGGCTCCGCGCCTAGACGGCGCCACCGCGGCAAGTTTCACCACAC
CCTCCACGGTAAAAAGCCCAGCTCCACGCATCGAACCTGCCGGGCCTTTCACGCGCGCGCTGTGGGAGGCAAGTGGCGAT
ATCATCGCGGGCATCAACAGTTCTGACTTCATCACCATGCTTGGCGACGGCACCCTCCGGCGCCCAGAGTTCGATTTCTA
CATCGACCAAGACGCCCAATACCTCGCCCAATATTCCCGAGCATTGGCACGACTCTCTTCGATCGCACCTGATTCACACG
CACAAATCGAATGGGCTCAAAGCGCTGCAGAATGCCTGGTCGTGGAAGCCGAACTGCACCGCTCTTATATGGCAGGGAAA
GAAGTCTCCGCACCATCACACATCACGATGGCCTATACCGACTTCCTCATCGCACGCACCTACACTGAGGACTACGTCTG
CGGTGTGGCGGCCGTACTGCCGTGCTACTGGCTCTACGCAGAGATCGGATTGATGCTGGCTGAACAAAACCACGACGAGC
ACCCCTACAAGGACTGGCTCAACACTTACTCCGGTGAAGAATTCATCGCTGGAACCCGTGCGGCTATCGCACGTCTAGAA
AAGGCTCTAGAAAATGCGGGTGCTGAACAACGCGTCGATGCTGCTCGAGCATTCTTGAGCGCCTCTGTTCATGAACGCGA
GTTCTTTGATCAGGCAACTCGCCATGGTTGGACAATGGTTGGATCTAGCTAA

Upstream 100 bases:

>100_bases
GCGCCAGGCAGCTTTGCGGTGGCGTTTATTGATGCGCTTTATGACGTGGATGCCCAGGCTGTGGCCTCGTTGGTTGATGT
GCGAGAGGCCTGAAAAGTAC

Downstream 100 bases:

>100_bases
ACCTGATCTCACTGCGCGCCGGGCCATCTCTGTCAATTAGGAGATGGCCCGGCGCGCTTTTGCAGCTGTGGGGTTGGGCT
GGGGGCAAGTGTTCATGTAG

Product: multifunctional thiamine-phosphate pyrophosphorylase/synthase/phosphomethylpyrimidine kinase

Products: NA

Alternate protein names: Thiamine-phosphate pyrophosphorylase; TMP pyrophosphorylase; TMP-PPase; Thiamine-phosphate synthase; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase; Hydroxymethylpyrimidine kinase; HMP kinase; Hydroxymethylpyrimidine phosphate kinase; HMP-P kinase; HMP-phosphate kinase; HMPP kinase

Number of amino acids: Translated: 763; Mature: 762

Protein sequence:

>763_residues
MTDFSLYLVTDPVLGGGPEKVAGIVDSAISGGVSVVQLRDKNSGVEDVRAAAKELKELCDARGVALVVNDYLDIAVELGL
HLHIGQGDTPYTQARELLPAHLELGLSIENLDQLHAVIAQCAETGVALPDVIGIGPVASTATKPDAAPALGVEGIAEIAA
VAQDHGIASVAIGGVGLRNAAELAATPIDGLCVVSEIMTAANPAAAATRLRTAFQPTFSPETQTELSQTELQGAFVNSPS
APRVLSIAGTDPTGGAGIQADLKSIAAGGGYGMCVVTSLVAQNTHGVNTIHTPPLTFLEEQLEAVFSDVTVDAIKLGMLG
SADTVDLVASWLGSHEHGPVVLDPVMIATSGDRLLDASAEESLRRLAVHVDVVTPNIPELAVLCDSAPAITMDEAIAQAQ
GFARTHDTIVIVKGGHLTGALADNAVVRPDGSVFQVENLRVNTTNSHGTGCSLSASLATKIAAGESVEKALEWSTRWLNE
ALRHADHLAVGTGNGPVDHGHLARRMTHAAETTPWAHLRAPRLDGATAASFTTPSTVKSPAPRIEPAGPFTRALWEASGD
IIAGINSSDFITMLGDGTLRRPEFDFYIDQDAQYLAQYSRALARLSSIAPDSHAQIEWAQSAAECLVVEAELHRSYMAGK
EVSAPSHITMAYTDFLIARTYTEDYVCGVAAVLPCYWLYAEIGLMLAEQNHDEHPYKDWLNTYSGEEFIAGTRAAIARLE
KALENAGAEQRVDAARAFLSASVHEREFFDQATRHGWTMVGSS

Sequences:

>Translated_763_residues
MTDFSLYLVTDPVLGGGPEKVAGIVDSAISGGVSVVQLRDKNSGVEDVRAAAKELKELCDARGVALVVNDYLDIAVELGL
HLHIGQGDTPYTQARELLPAHLELGLSIENLDQLHAVIAQCAETGVALPDVIGIGPVASTATKPDAAPALGVEGIAEIAA
VAQDHGIASVAIGGVGLRNAAELAATPIDGLCVVSEIMTAANPAAAATRLRTAFQPTFSPETQTELSQTELQGAFVNSPS
APRVLSIAGTDPTGGAGIQADLKSIAAGGGYGMCVVTSLVAQNTHGVNTIHTPPLTFLEEQLEAVFSDVTVDAIKLGMLG
SADTVDLVASWLGSHEHGPVVLDPVMIATSGDRLLDASAEESLRRLAVHVDVVTPNIPELAVLCDSAPAITMDEAIAQAQ
GFARTHDTIVIVKGGHLTGALADNAVVRPDGSVFQVENLRVNTTNSHGTGCSLSASLATKIAAGESVEKALEWSTRWLNE
ALRHADHLAVGTGNGPVDHGHLARRMTHAAETTPWAHLRAPRLDGATAASFTTPSTVKSPAPRIEPAGPFTRALWEASGD
IIAGINSSDFITMLGDGTLRRPEFDFYIDQDAQYLAQYSRALARLSSIAPDSHAQIEWAQSAAECLVVEAELHRSYMAGK
EVSAPSHITMAYTDFLIARTYTEDYVCGVAAVLPCYWLYAEIGLMLAEQNHDEHPYKDWLNTYSGEEFIAGTRAAIARLE
KALENAGAEQRVDAARAFLSASVHEREFFDQATRHGWTMVGSS
>Mature_762_residues
TDFSLYLVTDPVLGGGPEKVAGIVDSAISGGVSVVQLRDKNSGVEDVRAAAKELKELCDARGVALVVNDYLDIAVELGLH
LHIGQGDTPYTQARELLPAHLELGLSIENLDQLHAVIAQCAETGVALPDVIGIGPVASTATKPDAAPALGVEGIAEIAAV
AQDHGIASVAIGGVGLRNAAELAATPIDGLCVVSEIMTAANPAAAATRLRTAFQPTFSPETQTELSQTELQGAFVNSPSA
PRVLSIAGTDPTGGAGIQADLKSIAAGGGYGMCVVTSLVAQNTHGVNTIHTPPLTFLEEQLEAVFSDVTVDAIKLGMLGS
ADTVDLVASWLGSHEHGPVVLDPVMIATSGDRLLDASAEESLRRLAVHVDVVTPNIPELAVLCDSAPAITMDEAIAQAQG
FARTHDTIVIVKGGHLTGALADNAVVRPDGSVFQVENLRVNTTNSHGTGCSLSASLATKIAAGESVEKALEWSTRWLNEA
LRHADHLAVGTGNGPVDHGHLARRMTHAAETTPWAHLRAPRLDGATAASFTTPSTVKSPAPRIEPAGPFTRALWEASGDI
IAGINSSDFITMLGDGTLRRPEFDFYIDQDAQYLAQYSRALARLSSIAPDSHAQIEWAQSAAECLVVEAELHRSYMAGKE
VSAPSHITMAYTDFLIARTYTEDYVCGVAAVLPCYWLYAEIGLMLAEQNHDEHPYKDWLNTYSGEEFIAGTRAAIARLEK
ALENAGAEQRVDAARAFLSASVHEREFFDQATRHGWTMVGSS

Specific function: Catalyzes the phosphorylation of hydroxymethylpyrimidine phosphate (HMP-P) to HMP-PP, and of HMP to HMP-P

COG id: COG0351

COG function: function code H; Hydroxymethylpyrimidine/phosphomethylpyrimidine kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: In the C-terminal section; belongs to the thiaminase-2 family

Homologues:

Organism=Escherichia coli, GI1788420, Length=258, Percent_Identity=44.5736434108527, Blast_Score=194, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6325378, Length=506, Percent_Identity=26.0869565217391, Blast_Score=140, Evalue=6e-34,
Organism=Saccharomyces cerevisiae, GI6324517, Length=391, Percent_Identity=32.4808184143223, Blast_Score=138, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6324997, Length=397, Percent_Identity=31.4861460957179, Blast_Score=136, Evalue=1e-32,
Organism=Saccharomyces cerevisiae, GI6325042, Length=258, Percent_Identity=29.0697674418605, Blast_Score=97, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): THIED_CORGL (Q8NQH1)

Other databases:

- EMBL:   BA000036
- EMBL:   BX927152
- RefSeq:   NP_600680.3
- RefSeq:   YP_225748.1
- ProteinModelPortal:   Q8NQH1
- GeneID:   1019437
- GeneID:   3345397
- GenomeReviews:   BA000036_GR
- GenomeReviews:   BX927147_GR
- KEGG:   cgb:cg1654
- KEGG:   cgl:NCgl1407
- HOGENOM:   HBG476673
- OMA:   KIGMLGD
- PhylomeDB:   Q8NQH1
- ProtClustDB:   PRK09517
- BioCyc:   CGLU196627:CG1654-MONOMER
- BRENDA:   2.5.1.3
- BRENDA:   2.7.4.7
- HAMAP:   MF_00097
- InterPro:   IPR013785
- InterPro:   IPR016084
- InterPro:   IPR013749
- InterPro:   IPR004399
- InterPro:   IPR004305
- InterPro:   IPR022998
- InterPro:   IPR003733
- Gene3D:   G3DSA:3.20.20.70
- Gene3D:   G3DSA:1.20.910.10
- TIGRFAMs:   TIGR00097
- TIGRFAMs:   TIGR00693

Pfam domain/function: PF08543 Phos_pyr_kin; PF03070 TENA_THI-4; PF02581 TMP-TENI; SSF48613 Heme_oxygenase; SSF51391 TMP_synthase

EC number: =2.5.1.3; =2.7.1.49; =2.7.4.7

Molecular weight: Translated: 80356; Mature: 80225

Theoretical pI: Translated: 4.57; Mature: 4.57

Prosite motif: NA

Important sites: BINDING 282-282

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDFSLYLVTDPVLGGGPEKVAGIVDSAISGGVSVVQLRDKNSGVEDVRAAAKELKELCD
CCCEEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHC
ARGVALVVNDYLDIAVELGLHLHIGQGDTPYTQARELLPAHLELGLSIENLDQLHAVIAQ
CCCEEEEEHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
CAETGVALPDVIGIGPVASTATKPDAAPALGVEGIAEIAAVAQDHGIASVAIGGVGLRNA
HHHCCCCCCHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCH
AELAATPIDGLCVVSEIMTAANPAAAATRLRTAFQPTFSPETQTELSQTELQGAFVNSPS
HHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCC
APRVLSIAGTDPTGGAGIQADLKSIAAGGGYGMCVVTSLVAQNTHGVNTIHTPPLTFLEE
CCEEEEEECCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHH
QLEAVFSDVTVDAIKLGMLGSADTVDLVASWLGSHEHGPVVLDPVMIATSGDRLLDASAE
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCEEECCEEEEECCCCCCCCHHH
ESLRRLAVHVDVVTPNIPELAVLCDSAPAITMDEAIAQAQGFARTHDTIVIVKGGHLTGA
HHHHHHEEEEEEECCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCEEEE
LADNAVVRPDGSVFQVENLRVNTTNSHGTGCSLSASLATKIAAGESVEKALEWSTRWLNE
CCCCEEECCCCCEEEEEEEEEECCCCCCCCCEEHHHHHHHHHCCHHHHHHHHHHHHHHHH
ALRHADHLAVGTGNGPVDHGHLARRMTHAAETTPWAHLRAPRLDGATAASFTTPSTVKSP
HHHHCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCHHEECCCCCCCCCCCCCCCCCCCCC
APRIEPAGPFTRALWEASGDIIAGINSSDFITMLGDGTLRRPEFDFYIDQDAQYLAQYSR
CCCCCCCCHHHHHHHHCCCCEEEECCCCCEEEEECCCCCCCCCCCEEECCHHHHHHHHHH
ALARLSSIAPDSHAQIEWAQSAAECLVVEAELHRSYMAGKEVSAPSHITMAYTDFLIART
HHHHHHHCCCCCCCHHHHHHHHHHEEEEHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHH
YTEDYVCGVAAVLPCYWLYAEIGLMLAEQNHDEHPYKDWLNTYSGEEFIAGTRAAIARLE
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHH
KALENAGAEQRVDAARAFLSASVHEREFFDQATRHGWTMVGSS
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCC
>Mature Secondary Structure 
TDFSLYLVTDPVLGGGPEKVAGIVDSAISGGVSVVQLRDKNSGVEDVRAAAKELKELCD
CCEEEEEEECCCCCCCHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHC
ARGVALVVNDYLDIAVELGLHLHIGQGDTPYTQARELLPAHLELGLSIENLDQLHAVIAQ
CCCEEEEEHHHHHHHHHCCEEEEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHH
CAETGVALPDVIGIGPVASTATKPDAAPALGVEGIAEIAAVAQDHGIASVAIGGVGLRNA
HHHCCCCCCHHHCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCCH
AELAATPIDGLCVVSEIMTAANPAAAATRLRTAFQPTFSPETQTELSQTELQGAFVNSPS
HHHHCCCCCHHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCCCCC
APRVLSIAGTDPTGGAGIQADLKSIAAGGGYGMCVVTSLVAQNTHGVNTIHTPPLTFLEE
CCEEEEEECCCCCCCCCHHHHHHHHHCCCCCHHHHHHHHHHCCCCCCCCCCCCCHHHHHH
QLEAVFSDVTVDAIKLGMLGSADTVDLVASWLGSHEHGPVVLDPVMIATSGDRLLDASAE
HHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCEEECCEEEEECCCCCCCCHHH
ESLRRLAVHVDVVTPNIPELAVLCDSAPAITMDEAIAQAQGFARTHDTIVIVKGGHLTGA
HHHHHHEEEEEEECCCCCCEEEEECCCCCCHHHHHHHHHHCCCCCCCEEEEEECCCEEEE
LADNAVVRPDGSVFQVENLRVNTTNSHGTGCSLSASLATKIAAGESVEKALEWSTRWLNE
CCCCEEECCCCCEEEEEEEEEECCCCCCCCCEEHHHHHHHHHCCHHHHHHHHHHHHHHHH
ALRHADHLAVGTGNGPVDHGHLARRMTHAAETTPWAHLRAPRLDGATAASFTTPSTVKSP
HHHHCCCEEEECCCCCCCHHHHHHHHHHHHCCCCCHHEECCCCCCCCCCCCCCCCCCCCC
APRIEPAGPFTRALWEASGDIIAGINSSDFITMLGDGTLRRPEFDFYIDQDAQYLAQYSR
CCCCCCCCHHHHHHHHCCCCEEEECCCCCEEEEECCCCCCCCCCCEEECCHHHHHHHHHH
ALARLSSIAPDSHAQIEWAQSAAECLVVEAELHRSYMAGKEVSAPSHITMAYTDFLIART
HHHHHHHCCCCCCCHHHHHHHHHHEEEEHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHHH
YTEDYVCGVAAVLPCYWLYAEIGLMLAEQNHDEHPYKDWLNTYSGEEFIAGTRAAIARLE
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCCCHHHHHHHHHHHHHH
KALENAGAEQRVDAARAFLSASVHEREFFDQATRHGWTMVGSS
HHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12948626