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The map label for this gene is lysE

Identifier: 62390149

GI number: 62390149

Start: 1329712

End: 1330413

Strand: Reverse

Name: lysE

Synonym: cg1424

Alternate gene names: 62390149

Gene position: 1330413-1329712 (Counterclockwise)

Preceding gene: 62390151

Following gene: 62390136

Centisome position: 40.53

GC content: 55.7

Gene sequence:

>702_bases
ATGGAAATCTTCATTACAGGTCTGCTTTTGGGGGCCAGTCTTTTACTGTCCATCGGACCGCAGAATGTACTGGTGATTAA
ACAAGGAATTAAGCGCGAAGGACTCATTGCGGTTCTTCTCGTGTGTTTAATTTCTGACGTCTTTTTGTTCATCGCCGGCA
CCTTGGGCGTTGATCTTTTGTCCAATGCCGCGCCGATCGTGCTCGATATTATGCGCTGGGGTGGCATCGCTTACCTGTTA
TGGTTTGCCGTCATGGCAGCGAAAGACGCCATGACAAACAAGGTGGAAGCGCCACAGATCATTGAAGAAACAGAACCAAC
CGTGCCCGATGACACGCCTTTGGGCGGTTCGGCGGTGGCCACTGACACGCGCAACCGGGTGCGGGTGGAGGTGAGCGTCG
ATAAGCAGCGGGTTTGGGTAAAGCCCATGTTGATGGCAATCGTGCTGACCTGGTTGAACCCGAATGCGTATTTGGACGCG
TTTGTGTTTATCGGCGGCGTCGGCGCGCAATACGGCGACACCGGACGGTGGATTTTCGCCGCTGGCGCGTTCGCGGCAAG
CCTGATCTGGTTCCCGCTGGTGGGTTTCGGCGCAGCAGCATTGTCACGCCCGCTGTCCAGCCCCAAGGTGTGGCGCTGGA
TCAACGTCGTCGTGGCAGTTGTGATGACCGCATTGGCCATCAAACTGATGTTGATGGGTTAG

Upstream 100 bases:

>100_bases
GAGCAAAGTGTCCAGTTGAATGGGGTTCATGAAGCTATATTAAACCATGTTAAGAACCAATCATTTTACTTAAGTACTTC
CATAGGTCACGATGGTGATC

Downstream 100 bases:

>100_bases
TTTTCGCGGGTTTTGGAATCGGTGGCCTTCGCCCAAATGTTGATGCCGGCGTCGTGGGAAATCTCATCGATCGCCTCCAA
CTCGGCGTCAGAAAACTCCA

Product: lysine efflux permease

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 233; Mature: 233

Protein sequence:

>233_residues
MEIFITGLLLGASLLLSIGPQNVLVIKQGIKREGLIAVLLVCLISDVFLFIAGTLGVDLLSNAAPIVLDIMRWGGIAYLL
WFAVMAAKDAMTNKVEAPQIIEETEPTVPDDTPLGGSAVATDTRNRVRVEVSVDKQRVWVKPMLMAIVLTWLNPNAYLDA
FVFIGGVGAQYGDTGRWIFAAGAFAASLIWFPLVGFGAAALSRPLSSPKVWRWINVVVAVVMTALAIKLMLMG

Sequences:

>Translated_233_residues
MEIFITGLLLGASLLLSIGPQNVLVIKQGIKREGLIAVLLVCLISDVFLFIAGTLGVDLLSNAAPIVLDIMRWGGIAYLL
WFAVMAAKDAMTNKVEAPQIIEETEPTVPDDTPLGGSAVATDTRNRVRVEVSVDKQRVWVKPMLMAIVLTWLNPNAYLDA
FVFIGGVGAQYGDTGRWIFAAGAFAASLIWFPLVGFGAAALSRPLSSPKVWRWINVVVAVVMTALAIKLMLMG
>Mature_233_residues
MEIFITGLLLGASLLLSIGPQNVLVIKQGIKREGLIAVLLVCLISDVFLFIAGTLGVDLLSNAAPIVLDIMRWGGIAYLL
WFAVMAAKDAMTNKVEAPQIIEETEPTVPDDTPLGGSAVATDTRNRVRVEVSVDKQRVWVKPMLMAIVLTWLNPNAYLDA
FVFIGGVGAQYGDTGRWIFAAGAFAASLIWFPLVGFGAAALSRPLSSPKVWRWINVVVAVVMTALAIKLMLMG

Specific function: Involved in the efflux of excess of L-lysine. This is necessary to control the intracellular L-lysine level

COG id: COG1279

COG function: function code R; Lysine efflux permease

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the LysE/ArgO transporter (TC 2.A.75) family

Homologues:

Organism=Escherichia coli, GI1789290, Length=229, Percent_Identity=35.3711790393013, Blast_Score=117, Evalue=7e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): LYSE_CORGL (P94633)

Other databases:

- EMBL:   X96471
- EMBL:   BA000036
- EMBL:   BX927151
- RefSeq:   NP_600485.1
- RefSeq:   YP_225551.1
- GeneID:   1019244
- GeneID:   3343730
- GenomeReviews:   BA000036_GR
- GenomeReviews:   BX927147_GR
- KEGG:   cgb:cg1424
- KEGG:   cgl:NCgl1214
- HOGENOM:   HBG683136
- OMA:   EHHLPVA
- PhylomeDB:   P94633
- ProtClustDB:   CLSK633306
- BioCyc:   CGLU196627:CG1424-MONOMER
- InterPro:   IPR004777
- InterPro:   IPR001123
- TIGRFAMs:   TIGR00948

Pfam domain/function: PF01810 LysE

EC number: NA

Molecular weight: Translated: 25082; Mature: 25082

Theoretical pI: Translated: 6.75; Mature: 6.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

HASH(0x1045597c)-; HASH(0x135f2658)-; HASH(0x136bd63c)-; HASH(0x128e2208)-; HASH(0x131eb284)-; HASH(0x404057ac)-;

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.9 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.9 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIFITGLLLGASLLLSIGPQNVLVIKQGIKREGLIAVLLVCLISDVFLFIAGTLGVDLL
CEEEHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SNAAPIVLDIMRWGGIAYLLWFAVMAAKDAMTNKVEAPQIIEETEPTVPDDTPLGGSAVA
CCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCCCEEE
TDTRNRVRVEVSVDKQRVWVKPMLMAIVLTWLNPNAYLDAFVFIGGVGAQYGDTGRWIFA
CCCCCEEEEEEEECCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCEEEE
AGAFAASLIWFPLVGFGAAALSRPLSSPKVWRWINVVVAVVMTALAIKLMLMG
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCH
>Mature Secondary Structure
MEIFITGLLLGASLLLSIGPQNVLVIKQGIKREGLIAVLLVCLISDVFLFIAGTLGVDLL
CEEEHHHHHHHHHHHHHCCCCCEEEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHH
SNAAPIVLDIMRWGGIAYLLWFAVMAAKDAMTNKVEAPQIIEETEPTVPDDTPLGGSAVA
CCCCHHHHHHHHHCHHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCCCCCCCCCCEEE
TDTRNRVRVEVSVDKQRVWVKPMLMAIVLTWLNPNAYLDAFVFIGGVGAQYGDTGRWIFA
CCCCCEEEEEEEECCHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCCCCCCCCEEEE
AGAFAASLIWFPLVGFGAAALSRPLSSPKVWRWINVVVAVVMTALAIKLMLMG
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCH

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8971704; 12948626