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The map label for this gene is aroE [C]

Identifier: 62390022

GI number: 62390022

Start: 1182337

End: 1183143

Strand: Reverse

Name: aroE [C]

Synonym: cg1283

Alternate gene names: 62390022

Gene position: 1183143-1182337 (Counterclockwise)

Preceding gene: 62390026

Following gene: 62390021

Centisome position: 36.04

GC content: 52.79

Gene sequence:

>807_bases
ATGGTCAACTACGTCGACAGGGAAACAACCCTGTGCATCTCTCTCGCTGCTCGTCCATCCAACCATGGTGTTCGTTTCCA
CAACTGGCTTTACGCTGAACTTGGATTGAACTACCTGTACAAGGCTGTTGCCCCAGCAGATATCACCGCTGCAGTCGCAG
GTATCCGTGGTCTGAACATTCGCGGCGCAGGTGTCTCCATGCCATACAAGAGCGATGTCATCCCACTCATCGATGAGTTG
CATCCTTCCGCAGAGCGCATACGTTCTGTTAACACCATCGTCAACAATGACGGACACCTTGTCGGATACAACACCGACTA
CACTGCGGTGTACCACCTCCTTGAAGAACACCGCGTGAACCCCAATGCACGAGTAGCTATCAAGGGATCCGGCGGCATGG
CCAATGCTGTTGTTGCAGCTCTTGCTGAGTATGGTCTGAGTGGCACCGTCGTTGCCCGCAACCACACCACCGGTTCTGCG
CTAGCTTCCCGTTACGGTTGGGAATACTCCGCAACTGTTCCGGAAGACGCAAAAATTTTGGTTAATGTAACCCCAATGGG
AATGAATGGACCTGACCAAGACGTTGTATCTTTTGGTGAGGATGAAGTAGACCGAGCCGACGTAATCTTTGACTGCGTAG
CATTCCCCGTCGAGACCCCACTGATTAAGTTGGCCAAGGAAAAGGGTAAGCAAACCATCGACGGCGGAGAAGTTGCCGCT
CTTCAGGCAGCAGAGCAGTTCCACCTCTACACCGGAGTTCTTCCAACCAACGACCAGATCATTGCTGCGGAGGAGTTCTC
CAAGTAA

Upstream 100 bases:

>100_bases
GGCAGGAATTTCCCGAAAACTTCCACCAATAATCAAGCCATATCCCACACAATCAGGCATTTGCTCTTCCTTTGCTCCGC
ATGAGTATAAAATCACTGTC

Downstream 100 bases:

>100_bases
ATTTCTCTCCCCTATTTTTAGGAGGCACCACATGGCTGAACACAACGCCATCATCACGGATGCAGTACATTCCGACCCCG
CTGTTTTAGAAGACAACGCC

Product: shikimate 5-dehydrogenase

Products: 3-dehydroshikimate; NADPH; H+

Alternate protein names: SDH-L [H]

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MVNYVDRETTLCISLAARPSNHGVRFHNWLYAELGLNYLYKAVAPADITAAVAGIRGLNIRGAGVSMPYKSDVIPLIDEL
HPSAERIRSVNTIVNNDGHLVGYNTDYTAVYHLLEEHRVNPNARVAIKGSGGMANAVVAALAEYGLSGTVVARNHTTGSA
LASRYGWEYSATVPEDAKILVNVTPMGMNGPDQDVVSFGEDEVDRADVIFDCVAFPVETPLIKLAKEKGKQTIDGGEVAA
LQAAEQFHLYTGVLPTNDQIIAAEEFSK

Sequences:

>Translated_268_residues
MVNYVDRETTLCISLAARPSNHGVRFHNWLYAELGLNYLYKAVAPADITAAVAGIRGLNIRGAGVSMPYKSDVIPLIDEL
HPSAERIRSVNTIVNNDGHLVGYNTDYTAVYHLLEEHRVNPNARVAIKGSGGMANAVVAALAEYGLSGTVVARNHTTGSA
LASRYGWEYSATVPEDAKILVNVTPMGMNGPDQDVVSFGEDEVDRADVIFDCVAFPVETPLIKLAKEKGKQTIDGGEVAA
LQAAEQFHLYTGVLPTNDQIIAAEEFSK
>Mature_268_residues
MVNYVDRETTLCISLAARPSNHGVRFHNWLYAELGLNYLYKAVAPADITAAVAGIRGLNIRGAGVSMPYKSDVIPLIDEL
HPSAERIRSVNTIVNNDGHLVGYNTDYTAVYHLLEEHRVNPNARVAIKGSGGMANAVVAALAEYGLSGTVVARNHTTGSA
LASRYGWEYSATVPEDAKILVNVTPMGMNGPDQDVVSFGEDEVDRADVIFDCVAFPVETPLIKLAKEKGKQTIDGGEVAA
LQAAEQFHLYTGVLPTNDQIIAAEEFSK

Specific function: The physiological substrate is not known. Has much lower activity towards shikimate than AroE [H]

COG id: COG0169

COG function: function code E; Shikimate 5-dehydrogenase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the shikimate dehydrogenase family [H]

Homologues:

Organism=Escherichia coli, GI1787983, Length=263, Percent_Identity=31.1787072243346, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1789675, Length=219, Percent_Identity=26.9406392694064, Blast_Score=68, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR013708
- InterPro:   IPR006151 [H]

Pfam domain/function: PF01488 Shikimate_DH; PF08501 Shikimate_dh_N [H]

EC number: 1.1.1.25

Molecular weight: Translated: 28871; Mature: 28871

Theoretical pI: Translated: 4.97; Mature: 4.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVNYVDRETTLCISLAARPSNHGVRFHNWLYAELGLNYLYKAVAPADITAAVAGIRGLNI
CCCCCCCCEEEEEEEEECCCCCCEEEHHEEEEHHHHHHHHHHCCCCHHHHHHHHCCCCEE
RGAGVSMPYKSDVIPLIDELHPSAERIRSVNTIVNNDGHLVGYNTDYTAVYHLLEEHRVN
ECCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHEECCCCEEEEECCCHHHHHHHHHHHCCC
PNARVAIKGSGGMANAVVAALAEYGLSGTVVARNHTTGSALASRYGWEYSATVPEDAKIL
CCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHCCCEEECCCCCCCEEE
VNVTPMGMNGPDQDVVSFGEDEVDRADVIFDCVAFPVETPLIKLAKEKGKQTIDGGEVAA
EEEEECCCCCCCHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCHH
LQAAEQFHLYTGVLPTNDQIIAAEEFSK
HHHHHHEEEEECCCCCCCCEEECCCCCC
>Mature Secondary Structure
MVNYVDRETTLCISLAARPSNHGVRFHNWLYAELGLNYLYKAVAPADITAAVAGIRGLNI
CCCCCCCCEEEEEEEEECCCCCCEEEHHEEEEHHHHHHHHHHCCCCHHHHHHHHCCCCEE
RGAGVSMPYKSDVIPLIDELHPSAERIRSVNTIVNNDGHLVGYNTDYTAVYHLLEEHRVN
ECCCCCCCCCCCCHHHHHHCCCHHHHHHHHHHEECCCCEEEEECCCHHHHHHHHHHHCCC
PNARVAIKGSGGMANAVVAALAEYGLSGTVVARNHTTGSALASRYGWEYSATVPEDAKIL
CCCEEEEEECCCHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHCCCEEECCCCCCCEEE
VNVTPMGMNGPDQDVVSFGEDEVDRADVIFDCVAFPVETPLIKLAKEKGKQTIDGGEVAA
EEEEECCCCCCCHHHHHCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHCCCCCCCCCCCHH
LQAAEQFHLYTGVLPTNDQIIAAEEFSK
HHHHHHEEEEECCCCCCCCEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: shikimate; NADP+

Specific reaction: shikimate + NADP+ = 3-dehydroshikimate + NADPH + H+

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]