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The map label for this gene is menE [H]

Identifier: 62389350

GI number: 62389350

Start: 473810

End: 475036

Strand: Reverse

Name: menE [H]

Synonym: cg0533

Alternate gene names: 62389350

Gene position: 475036-473810 (Counterclockwise)

Preceding gene: 62389351

Following gene: 62389348

Centisome position: 14.47

GC content: 52.81

Gene sequence:

>1227_bases
GTGCATGGAGCATCGACGGGGTTCTGGCAAAACGCAAGGCCTAAATCTAGCGCCACAACTCCGAATTCTGAACCATCGGC
ACTAGAATCTCGGAATATGAATACTCGCGTCCTCGAAGCACTACCTGTTGATCTTGCAGATCCCACCGCAATTCTGGGAG
ATCTCGAGGACGCAATCTCTGGGAAGAAAACTTTCCTCCCCATCCCTGTACAAGATAAAACCCGTGCACAGTTGCTGCGC
GATTCTCAACGAGTTGGCCTCGCCATCGATCCTTCGATCGCTTTGGTGATGGCCACTTCTGGTTCTACAGGTACCCCGAA
GGGCGCTCAGCTCACTCCGTTGAATTTGGTGAGTTCCGCCGATGCTACGCATCAGTTTTTAGGTGGCGAAGGCCAGTGGT
TGCTTGCCATGCCAGCACACCACATTGCAGGCATGCAGGTGCTTCTTCGAAGCCTCATTGCTGGAGTTGAGCCACTAGCT
ATTGATCTCAGCACAGGTTTTCACATTGACGCTTTCGCAGGCGCCGCGGCAGAACTGAAAAATACCGGCGACCGCGTCTA
TACATCCTTGACTCCAATGCAGTTACTTAAAGCAATGGACTCCTTGCAAGGCATTGAAGCCCTGAAACTTTTTGATGTCA
TTCTTGTTGGCGGTGCTGCATTGTCTAAGCAGGCCCGAATTTCTGCGGAGCAGCTAGACATCAACATTGTCACCACCTAC
GGCTCCTCAGAGACTTCAGGTGGCTGCGTTTATGATGGCAAGCCCATTCCCGGCGCGAAAGTCCGTATTTCGGATGAGCG
CATTGAGTTGGGTGGCCCGATGATTGCGCAGGGCTACAGAAATGCACCTGAACATCCGGATTTCGCCAACGAGGGTTGGT
TTACCACCTCTGATTCAGGTGAACTCCACGACGGGATTCTCACCGTGACTGGTCGCGTGGATACCGTCATTGATTCCGGT
GGATTGAAGTTGCACCCAGAGGTACTGGAACGTGCCATCGCAGATATTAAAGGTGTCACCGCGGCGTGTGTTGTGGGTAT
TCCCGATCCCCGATTAGGCCAAGCAATTGTGGCCGCGTACTCCGGATCGATCAGTCCGTCTGAAGTTATTGAAGGCCTCG
ACGATCTACCTCGTTGGCAGCTTCCCAAACGGCTGAAGCATCTGGAATCTTTGCCCAGCATTGGTCCTGGAAAAGCTGAT
CGACGTGCTATCGCGAAGCTGTTTTAG

Upstream 100 bases:

>100_bases
TTTGCGCACGTCTCCTCCGGCATCTTTGTCACCAACAATGGTTGGGAACTCACCGGCGCAATCGGCGCTGGCGCGCTGCT
TCTCATCGCAGTTGGCGCAG

Downstream 100 bases:

>100_bases
TCTTCATTCTTGCTGGCTGCAACTAGTTTTGCCACATCTTCATCGGTGTACACTTTGGCGATCTGCTCATCATTTCCACC
CATGAGGGTGTTGCCAACAA

Product: O-succinylbenzoic acid--CoA ligase

Products: NA

Alternate protein names: OSB-CoA synthetase; o-succinylbenzoyl-CoA synthetase [H]

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MHGASTGFWQNARPKSSATTPNSEPSALESRNMNTRVLEALPVDLADPTAILGDLEDAISGKKTFLPIPVQDKTRAQLLR
DSQRVGLAIDPSIALVMATSGSTGTPKGAQLTPLNLVSSADATHQFLGGEGQWLLAMPAHHIAGMQVLLRSLIAGVEPLA
IDLSTGFHIDAFAGAAAELKNTGDRVYTSLTPMQLLKAMDSLQGIEALKLFDVILVGGAALSKQARISAEQLDINIVTTY
GSSETSGGCVYDGKPIPGAKVRISDERIELGGPMIAQGYRNAPEHPDFANEGWFTTSDSGELHDGILTVTGRVDTVIDSG
GLKLHPEVLERAIADIKGVTAACVVGIPDPRLGQAIVAAYSGSISPSEVIEGLDDLPRWQLPKRLKHLESLPSIGPGKAD
RRAIAKLF

Sequences:

>Translated_408_residues
MHGASTGFWQNARPKSSATTPNSEPSALESRNMNTRVLEALPVDLADPTAILGDLEDAISGKKTFLPIPVQDKTRAQLLR
DSQRVGLAIDPSIALVMATSGSTGTPKGAQLTPLNLVSSADATHQFLGGEGQWLLAMPAHHIAGMQVLLRSLIAGVEPLA
IDLSTGFHIDAFAGAAAELKNTGDRVYTSLTPMQLLKAMDSLQGIEALKLFDVILVGGAALSKQARISAEQLDINIVTTY
GSSETSGGCVYDGKPIPGAKVRISDERIELGGPMIAQGYRNAPEHPDFANEGWFTTSDSGELHDGILTVTGRVDTVIDSG
GLKLHPEVLERAIADIKGVTAACVVGIPDPRLGQAIVAAYSGSISPSEVIEGLDDLPRWQLPKRLKHLESLPSIGPGKAD
RRAIAKLF
>Mature_408_residues
MHGASTGFWQNARPKSSATTPNSEPSALESRNMNTRVLEALPVDLADPTAILGDLEDAISGKKTFLPIPVQDKTRAQLLR
DSQRVGLAIDPSIALVMATSGSTGTPKGAQLTPLNLVSSADATHQFLGGEGQWLLAMPAHHIAGMQVLLRSLIAGVEPLA
IDLSTGFHIDAFAGAAAELKNTGDRVYTSLTPMQLLKAMDSLQGIEALKLFDVILVGGAALSKQARISAEQLDINIVTTY
GSSETSGGCVYDGKPIPGAKVRISDERIELGGPMIAQGYRNAPEHPDFANEGWFTTSDSGELHDGILTVTGRVDTVIDSG
GLKLHPEVLERAIADIKGVTAACVVGIPDPRLGQAIVAAYSGSISPSEVIEGLDDLPRWQLPKRLKHLESLPSIGPGKAD
RRAIAKLF

Specific function: Converts 2-succinylbenzoate (OSB) to 2-succinylbenzoyl- CoA (OSB-CoA). May be involved in the biosynthesis of menaquinone [H]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family. MenE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1788595, Length=322, Percent_Identity=30.4347826086957, Blast_Score=118, Evalue=7e-28,
Organism=Escherichia coli, GI145693145, Length=373, Percent_Identity=25.4691689008043, Blast_Score=86, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17558820, Length=416, Percent_Identity=25.9615384615385, Blast_Score=84, Evalue=2e-16,
Organism=Caenorhabditis elegans, GI32563687, Length=337, Percent_Identity=27.5964391691395, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6319699, Length=355, Percent_Identity=24.5070422535211, Blast_Score=77, Evalue=6e-15,
Organism=Drosophila melanogaster, GI24653035, Length=402, Percent_Identity=24.8756218905473, Blast_Score=70, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.26 [H]

Molecular weight: Translated: 43038; Mature: 43038

Theoretical pI: Translated: 5.42; Mature: 5.42

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHGASTGFWQNARPKSSATTPNSEPSALESRNMNTRVLEALPVDLADPTAILGDLEDAIS
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCHHHHHHCCCCCCCCHHHHHHHHHHHC
GKKTFLPIPVQDKTRAQLLRDSQRVGLAIDPSIALVMATSGSTGTPKGAQLTPLNLVSSA
CCCEEEECCCCCHHHHHHHHCCCCCCEEECCCEEEEEEECCCCCCCCCCEECCHHHHHCC
DATHQFLGGEGQWLLAMPAHHIAGMQVLLRSLIAGVEPLAIDLSTGFHIDAFAGAAAELK
CHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEHHCCHHHHHH
NTGDRVYTSLTPMQLLKAMDSLQGIEALKLFDVILVGGAALSKQARISAEQLDINIVTTY
CCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHEEEEEEEEE
GSSETSGGCVYDGKPIPGAKVRISDERIELGGPMIAQGYRNAPEHPDFANEGWFTTSDSG
CCCCCCCCEEECCCCCCCCEEEEECCEEECCCCEEHHCCCCCCCCCCCCCCCCEECCCCC
ELHDGILTVTGRVDTVIDSGGLKLHPEVLERAIADIKGVTAACVVGIPDPRLGQAIVAAY
CCCCCEEEEECCEEEEECCCCCEECHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHH
SGSISPSEVIEGLDDLPRWQLPKRLKHLESLPSIGPGKADRRAIAKLF
CCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MHGASTGFWQNARPKSSATTPNSEPSALESRNMNTRVLEALPVDLADPTAILGDLEDAIS
CCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCHHHHHHCCCCCCCCHHHHHHHHHHHC
GKKTFLPIPVQDKTRAQLLRDSQRVGLAIDPSIALVMATSGSTGTPKGAQLTPLNLVSSA
CCCEEEECCCCCHHHHHHHHCCCCCCEEECCCEEEEEEECCCCCCCCCCEECCHHHHHCC
DATHQFLGGEGQWLLAMPAHHIAGMQVLLRSLIAGVEPLAIDLSTGFHIDAFAGAAAELK
CHHHHHHCCCCCEEEECCCHHHHHHHHHHHHHHCCCCCEEEEECCCCEEEHHCCHHHHHH
NTGDRVYTSLTPMQLLKAMDSLQGIEALKLFDVILVGGAALSKQARISAEQLDINIVTTY
CCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCHHHEEEEEEEEE
GSSETSGGCVYDGKPIPGAKVRISDERIELGGPMIAQGYRNAPEHPDFANEGWFTTSDSG
CCCCCCCCEEECCCCCCCCEEEEECCEEECCCCEEHHCCCCCCCCCCCCCCCCEECCCCC
ELHDGILTVTGRVDTVIDSGGLKLHPEVLERAIADIKGVTAACVVGIPDPRLGQAIVAAY
CCCCCEEEEECCEEEEECCCCCEECHHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHH
SGSISPSEVIEGLDDLPRWQLPKRLKHLESLPSIGPGKADRRAIAKLF
CCCCCHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]