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The map label for this gene is purU [H]

Identifier: 62389281

GI number: 62389281

Start: 404504

End: 405418

Strand: Reverse

Name: purU [H]

Synonym: cg0457

Alternate gene names: 62389281

Gene position: 405418-404504 (Counterclockwise)

Preceding gene: 62389284

Following gene: 62389274

Centisome position: 12.35

GC content: 52.35

Gene sequence:

>915_bases
ATGACCCCGAGTTCTCCTGAAGTTCGTAATCGTCCGAGCGCTGCGCCTGAAGAGCGTCAGTTTGTGCTCACTTTTGGCTG
CCCTGACTCCACTGGAATTGTGGCGAAGTTGTCGTCGTTCCTAGCTGAGCGTGGGGGTTGGATTACTGAGGCTGGATATT
TCACGGATCCTGATTCGAATTGGTTCTTTACTCGTCAGGCGATTCGCGCTGAGTCGATTGATACCACGATTGAGCAGTTG
CGGGAGGAGTTCGCTCCGCTTGCGGAGGAGTTCGGCCCGAGGGCTAAGTGGAGTTTCACTGACACTGCGCAGGTGAAGAA
GGCTGTGTTGTTGGTGTCTAAGGAGGGCCACTGCTTGCACGATTTGTTAGGTCGTGTGGCTGAGAATGATTATCCGATGG
AAGTTGTTGCGGTTGTGGGTAACCATGAGAACTTGCGTTATATTGCGGAGAACCATAATGTTCCGTTTTTCCATGTGCCG
TTTCCTAAGGATGCGGTTGGTAAGCGGAAGGCGTTTGACCAGGTCGCTGAGATTGTGAATGGTTATGATCCGGATGCGAT
TGTTTTGGCTCGTTTTATGCAGATTTTGCCGCCGGATTTGTGTGAGATGTGGGCTGGTCGTGTGTTGAATATTCATCACA
GTTTCTTGCCGTCGTTTATGGGTGCGCGCCCGTATCATCAGGCGTATAGCCGTGGTGTGAAGTTGATTGGTGCGACCTGC
CATTATGCGACTGGGGATCTGGATGATGGTCCGATCATTGAGCAGGATGTTATTCGTGTGACGCATAAGGATACGCCGAC
TGAGATGCAGCGTTTGGGCCGCGATGCGGAGAAGCAGGTGCTGGCTCGCGGTTTGCGTTTCCACTTGGAGGACCGGGTGC
TGGTTTACGGTAACCGCACGGTTGTCTTTGATTAA

Upstream 100 bases:

>100_bases
AGTCAAGGATTTGGGCCATGGTGGAGCGGGAAATCGTCATATCCATACCCTACTTAGACCTGACTTAGTGTGGGAAAATT
TCCAGGGTAGAATGCAACGA

Downstream 100 bases:

>100_bases
GGCTTTTTGCTTTTCGACGCGACCCTAAACAAATGAGACCTACCCGAGGGTAGGTCTCATTTGTTTTGTGTTTAGTCTGT
GGTGGTTTCGCGTAGTTTTT

Product: formyltetrahydrofolate deformylase

Products: NA

Alternate protein names: Formyl-FH(4) hydrolase [H]

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MTPSSPEVRNRPSAAPEERQFVLTFGCPDSTGIVAKLSSFLAERGGWITEAGYFTDPDSNWFFTRQAIRAESIDTTIEQL
REEFAPLAEEFGPRAKWSFTDTAQVKKAVLLVSKEGHCLHDLLGRVAENDYPMEVVAVVGNHENLRYIAENHNVPFFHVP
FPKDAVGKRKAFDQVAEIVNGYDPDAIVLARFMQILPPDLCEMWAGRVLNIHHSFLPSFMGARPYHQAYSRGVKLIGATC
HYATGDLDDGPIIEQDVIRVTHKDTPTEMQRLGRDAEKQVLARGLRFHLEDRVLVYGNRTVVFD

Sequences:

>Translated_304_residues
MTPSSPEVRNRPSAAPEERQFVLTFGCPDSTGIVAKLSSFLAERGGWITEAGYFTDPDSNWFFTRQAIRAESIDTTIEQL
REEFAPLAEEFGPRAKWSFTDTAQVKKAVLLVSKEGHCLHDLLGRVAENDYPMEVVAVVGNHENLRYIAENHNVPFFHVP
FPKDAVGKRKAFDQVAEIVNGYDPDAIVLARFMQILPPDLCEMWAGRVLNIHHSFLPSFMGARPYHQAYSRGVKLIGATC
HYATGDLDDGPIIEQDVIRVTHKDTPTEMQRLGRDAEKQVLARGLRFHLEDRVLVYGNRTVVFD
>Mature_303_residues
TPSSPEVRNRPSAAPEERQFVLTFGCPDSTGIVAKLSSFLAERGGWITEAGYFTDPDSNWFFTRQAIRAESIDTTIEQLR
EEFAPLAEEFGPRAKWSFTDTAQVKKAVLLVSKEGHCLHDLLGRVAENDYPMEVVAVVGNHENLRYIAENHNVPFFHVPF
PKDAVGKRKAFDQVAEIVNGYDPDAIVLARFMQILPPDLCEMWAGRVLNIHHSFLPSFMGARPYHQAYSRGVKLIGATCH
YATGDLDDGPIIEQDVIRVTHKDTPTEMQRLGRDAEKQVLARGLRFHLEDRVLVYGNRTVVFD

Specific function: Produces Formate From Formyl-Tetrahydrofolate. Provides The Major Source Of Formate For The Purt-Dependent Synthesis Of 5'-Phosphoribosyl-N-Formylglycinamide (Fgar) During Aerobic Growth. Has A Role In Regulating The One-Carbon Pool. [C]

COG id: COG0788

COG function: function code F; Formyltetrahydrofolate hydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ACT domain [H]

Homologues:

Organism=Homo sapiens, GI4503915, Length=185, Percent_Identity=29.7297297297297, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI209869995, Length=185, Percent_Identity=29.7297297297297, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI209869993, Length=185, Percent_Identity=29.7297297297297, Blast_Score=82, Evalue=4e-16,
Organism=Escherichia coli, GI1787483, Length=282, Percent_Identity=40.0709219858156, Blast_Score=219, Evalue=2e-58,
Organism=Escherichia coli, GI1788846, Length=162, Percent_Identity=30.8641975308642, Blast_Score=79, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17567511, Length=179, Percent_Identity=30.7262569832402, Blast_Score=80, Evalue=9e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR002376
- InterPro:   IPR004810 [H]

Pfam domain/function: PF01842 ACT; PF00551 Formyl_trans_N [H]

EC number: =3.5.1.10 [H]

Molecular weight: Translated: 34256; Mature: 34125

Theoretical pI: Translated: 5.96; Mature: 5.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPSSPEVRNRPSAAPEERQFVLTFGCPDSTGIVAKLSSFLAERGGWITEAGYFTDPDSN
CCCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCEEECCEEECCCCC
WFFTRQAIRAESIDTTIEQLREEFAPLAEEFGPRAKWSFTDTAQVKKAVLLVSKEGHCLH
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHEEECCCCCHHH
DLLGRVAENDYPMEVVAVVGNHENLRYIAENHNVPFFHVPFPKDAVGKRKAFDQVAEIVN
HHHHHHHCCCCCEEEEEEECCCCCEEEEEECCCCCEEECCCCCHHHHHHHHHHHHHHHHC
GYDPDAIVLARFMQILPPDLCEMWAGRVLNIHHSFLPSFMGARPYHQAYSRGVKLIGATC
CCCCHHHHHHHHHHHCCHHHHHHHCCCEEHHHHHHHHHHHCCCHHHHHHHCCCEEEEEEE
HYATGDLDDGPIIEQDVIRVTHKDTPTEMQRLGRDAEKQVLARGLRFHLEDRVLVYGNRT
ECCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEECEE
VVFD
EEEC
>Mature Secondary Structure 
TPSSPEVRNRPSAAPEERQFVLTFGCPDSTGIVAKLSSFLAERGGWITEAGYFTDPDSN
CCCCCCCCCCCCCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCCEEECCEEECCCCC
WFFTRQAIRAESIDTTIEQLREEFAPLAEEFGPRAKWSFTDTAQVKKAVLLVSKEGHCLH
EEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHEEECCCCCHHH
DLLGRVAENDYPMEVVAVVGNHENLRYIAENHNVPFFHVPFPKDAVGKRKAFDQVAEIVN
HHHHHHHCCCCCEEEEEEECCCCCEEEEEECCCCCEEECCCCCHHHHHHHHHHHHHHHHC
GYDPDAIVLARFMQILPPDLCEMWAGRVLNIHHSFLPSFMGARPYHQAYSRGVKLIGATC
CCCCHHHHHHHHHHHCCHHHHHHHCCCEEHHHHHHHHHHHCCCHHHHHHHCCCEEEEEEE
HYATGDLDDGPIIEQDVIRVTHKDTPTEMQRLGRDAEKQVLARGLRFHLEDRVLVYGNRT
ECCCCCCCCCCCCHHHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHCCCCEEEEEECEE
VVFD
EEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]