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The map label for this gene is ptrB [C]

Identifier: 62389238

GI number: 62389238

Start: 357235

End: 359241

Strand: Reverse

Name: ptrB [C]

Synonym: cg0410

Alternate gene names: 62389238

Gene position: 359241-357235 (Counterclockwise)

Preceding gene: 62389239

Following gene: 62389237

Centisome position: 10.94

GC content: 58.3

Gene sequence:

>2007_bases
ATGACTGATTACACGTTCCTCGAAGACATTGACACCCCGGAAGCGCTCGCGTGGGCGGAAAAATGGTCGGGGGAAAGCGT
CGAAAAGCTAAAAAGCCCAGCCAAGGACGCCCTGGAAGCCAGGCTGCTGGCTGCGTTGGACACCGATGATCGCATTGCCT
ACGTGAGCCGGCGCGGTGAGAAGCTGTACAACTTTTGGCGGGACGCGCAGCATCCGCGTGGAGTGTGGCGCACGACCACG
TTGGAGTCGTATGAAAGTGACCAGCCGGAGTGGGACGTGCTCATTGATGTGGATGCGTTGGCGGAGGATGAGGGCGAAAA
CTGGGTATGGAAGGGCGCGGTTGTGCGCTCGCCGGAGTTTGATCGGGCGTTGGTGAAGTTCTCGCGGGGCGGGGCTGATG
CGACGGTGATTAGGGAGTTTGATCTGGCCACGGCTGCTTTCGTGGATGATTCGCCGTTTGAATTGGAGGAGGCGAAGTCC
GATGTCACGTGGGTTGATCTGGATACGTTGCTGGTGGGCACGGATACCGGCGAGGGGTCACTGACGGATTCTGGGTACCC
GGCGCGGGTGCTCACGTGGAAGCGTGGGACTCCGCTTGAGCAGGCGGAGTTGTTCTTTGAGGGGTCGCGTCAGGATGTGG
CGACTCATGCGTGGCGGGATTCAACACCTGGTTTTGAGCGGACGTTTGTGTCAAGGTCGTTGGATTTCTATAATTCGGAG
ACGTCGCTGGAAACCGAGGGTGGCCTGGTCAAGCTTGATGTGCCGACCGATTGCGATGTCATTGTGAAGAAGCAGTGGAT
TTTTGTGAGTCCTCGGACGGATTTCGCTGGGATTCCAGCAGGTGGCTTGGGAGTGCTGCTGTTAAAGGAGTTCCTTGAGG
GCGGGCGCGATTTTCAGCCTGTGTTTACGCCTACTGAGTCGACGTCGCTGCAGGGATTGGCCACGACAAAGAATTTCCTG
GTTTTAACGCTCCTTAATAATGTCTCCACAGAAATCGTCACAGTGCCGCTCAATGATCCGACAACGGAGCATGAACACAT
TGACCTCCCAGAGCATGTCACCGCGCATGTGGTTGCTACCTCCCCGTTGGATGGCGATGAAATTTGGGTGCAGGCAGCGA
GTTTCACCGAAGCGCCAACGTTGCTGCGTGCGGAGCTGCCTGGTGCGCTTGAGGCTGTGAAGAAGGCGCCGTTGCAGTTT
GAAAATGCTGGTCAGGAGACTCGTCAGCATTGGGCAACCTCGGCGGATGGAACGAAGATTCCGTACTTTATTACAGGAGC
CTTCGAGGAGGAACCACAAAACACCCTGGTCCACGCCTACGGCGGCTTCGAGGTTTCCCTTACCCCAAGCCACTCCCCGA
CCCGCGGCATCGCATGGTTGGAAAAGGGCTACTACTTTGTGGAAGCCAACCTGCGTGGTGGCGGTGAATTCGGTCCGGAA
TGGCATTCGCAGGCAACCAAGCTGAACCGCATGAAGGTGTGGGAGGATCACCGCGCGGTGCTCGCCGACCTTGTGGAGCG
CGGCTACGCAACGCCGGAGCAGATTGCGATTCGTGGCGGATCCAACGGTGGTTTGCTGACAAGTGGCGCGTTAACTCAGT
ACCCAGAAGCATTCGGTGCGGCAGTTGTGCAGGTGCCGTTGGCTGATATGTTGCGCTATCACACCTGGTCAGCGGGTGCT
TCGTGGATGGCGGAGTACGGCAACCCTGACGATCCGGAGGAACGGGCGGTGATTGAGCAGTACTCGCCGGTGCAGGCGGT
GGTGGGCGTCGAGAAGCGAATTTATCCACCCGCATTGGTGACGACCTCAACCCGGGACGACCGCGTCCACCCCGCGCACG
CGCGCCTTTTTGCTCAAGCTTTGCTTGATGCGGGCCAGGCCGTGGATTACTACGAAAACACCGAGGGCGGCCATGCCGGC
GCGGCGGATAACAAGCAGACCGCGTTTGTGGAATCGCTGATCTACACCTGGATCGAGAAGACTTTGGATCAGCAGGGTAG
CATTTAA

Upstream 100 bases:

>100_bases
TACTACTCGGTTCACGTTTACGTCGGCTGATCCAATTGGAGGCGCCCTCGGAAGCCGCCTTAAAAAACCTGCCGGTCAAA
AGATCACTAACCTGAACTTC

Downstream 100 bases:

>100_bases
TACCTATGATTATGCGAAGGCTGCGCTCCACCCCGGTCCCTGGTACACGCGATTCCTACACAGGAATTGATTTCAACTTA
GGCTTCCACATCCGACGCTA

Product: prolyl endopeptidase

Products: Hydrolyzed protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 668; Mature: 667

Protein sequence:

>668_residues
MTDYTFLEDIDTPEALAWAEKWSGESVEKLKSPAKDALEARLLAALDTDDRIAYVSRRGEKLYNFWRDAQHPRGVWRTTT
LESYESDQPEWDVLIDVDALAEDEGENWVWKGAVVRSPEFDRALVKFSRGGADATVIREFDLATAAFVDDSPFELEEAKS
DVTWVDLDTLLVGTDTGEGSLTDSGYPARVLTWKRGTPLEQAELFFEGSRQDVATHAWRDSTPGFERTFVSRSLDFYNSE
TSLETEGGLVKLDVPTDCDVIVKKQWIFVSPRTDFAGIPAGGLGVLLLKEFLEGGRDFQPVFTPTESTSLQGLATTKNFL
VLTLLNNVSTEIVTVPLNDPTTEHEHIDLPEHVTAHVVATSPLDGDEIWVQAASFTEAPTLLRAELPGALEAVKKAPLQF
ENAGQETRQHWATSADGTKIPYFITGAFEEEPQNTLVHAYGGFEVSLTPSHSPTRGIAWLEKGYYFVEANLRGGGEFGPE
WHSQATKLNRMKVWEDHRAVLADLVERGYATPEQIAIRGGSNGGLLTSGALTQYPEAFGAAVVQVPLADMLRYHTWSAGA
SWMAEYGNPDDPEERAVIEQYSPVQAVVGVEKRIYPPALVTTSTRDDRVHPAHARLFAQALLDAGQAVDYYENTEGGHAG
AADNKQTAFVESLIYTWIEKTLDQQGSI

Sequences:

>Translated_668_residues
MTDYTFLEDIDTPEALAWAEKWSGESVEKLKSPAKDALEARLLAALDTDDRIAYVSRRGEKLYNFWRDAQHPRGVWRTTT
LESYESDQPEWDVLIDVDALAEDEGENWVWKGAVVRSPEFDRALVKFSRGGADATVIREFDLATAAFVDDSPFELEEAKS
DVTWVDLDTLLVGTDTGEGSLTDSGYPARVLTWKRGTPLEQAELFFEGSRQDVATHAWRDSTPGFERTFVSRSLDFYNSE
TSLETEGGLVKLDVPTDCDVIVKKQWIFVSPRTDFAGIPAGGLGVLLLKEFLEGGRDFQPVFTPTESTSLQGLATTKNFL
VLTLLNNVSTEIVTVPLNDPTTEHEHIDLPEHVTAHVVATSPLDGDEIWVQAASFTEAPTLLRAELPGALEAVKKAPLQF
ENAGQETRQHWATSADGTKIPYFITGAFEEEPQNTLVHAYGGFEVSLTPSHSPTRGIAWLEKGYYFVEANLRGGGEFGPE
WHSQATKLNRMKVWEDHRAVLADLVERGYATPEQIAIRGGSNGGLLTSGALTQYPEAFGAAVVQVPLADMLRYHTWSAGA
SWMAEYGNPDDPEERAVIEQYSPVQAVVGVEKRIYPPALVTTSTRDDRVHPAHARLFAQALLDAGQAVDYYENTEGGHAG
AADNKQTAFVESLIYTWIEKTLDQQGSI
>Mature_667_residues
TDYTFLEDIDTPEALAWAEKWSGESVEKLKSPAKDALEARLLAALDTDDRIAYVSRRGEKLYNFWRDAQHPRGVWRTTTL
ESYESDQPEWDVLIDVDALAEDEGENWVWKGAVVRSPEFDRALVKFSRGGADATVIREFDLATAAFVDDSPFELEEAKSD
VTWVDLDTLLVGTDTGEGSLTDSGYPARVLTWKRGTPLEQAELFFEGSRQDVATHAWRDSTPGFERTFVSRSLDFYNSET
SLETEGGLVKLDVPTDCDVIVKKQWIFVSPRTDFAGIPAGGLGVLLLKEFLEGGRDFQPVFTPTESTSLQGLATTKNFLV
LTLLNNVSTEIVTVPLNDPTTEHEHIDLPEHVTAHVVATSPLDGDEIWVQAASFTEAPTLLRAELPGALEAVKKAPLQFE
NAGQETRQHWATSADGTKIPYFITGAFEEEPQNTLVHAYGGFEVSLTPSHSPTRGIAWLEKGYYFVEANLRGGGEFGPEW
HSQATKLNRMKVWEDHRAVLADLVERGYATPEQIAIRGGSNGGLLTSGALTQYPEAFGAAVVQVPLADMLRYHTWSAGAS
WMAEYGNPDDPEERAVIEQYSPVQAVVGVEKRIYPPALVTTSTRDDRVHPAHARLFAQALLDAGQAVDYYENTEGGHAGA
ADNKQTAFVESLIYTWIEKTLDQQGSI

Specific function: Cleaves Peptide Bonds On The C-Terminal Side Of Lysyl And Argininyl Residues. [C]

COG id: COG1505

COG function: function code E; Serine proteases of the peptidase family S9A

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S9B family [H]

Homologues:

Organism=Homo sapiens, GI41349456, Length=328, Percent_Identity=32.0121951219512, Blast_Score=167, Evalue=4e-41,
Organism=Homo sapiens, GI284172438, Length=205, Percent_Identity=25.8536585365854, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI284172431, Length=205, Percent_Identity=25.8536585365854, Blast_Score=79, Evalue=1e-14,
Organism=Homo sapiens, GI284172420, Length=205, Percent_Identity=25.8536585365854, Blast_Score=79, Evalue=2e-14,
Organism=Homo sapiens, GI284172413, Length=205, Percent_Identity=25.8536585365854, Blast_Score=79, Evalue=2e-14,
Organism=Homo sapiens, GI70778815, Length=205, Percent_Identity=25.8536585365854, Blast_Score=79, Evalue=2e-14,
Organism=Homo sapiens, GI108860686, Length=205, Percent_Identity=25.8536585365854, Blast_Score=78, Evalue=2e-14,
Organism=Homo sapiens, GI108860692, Length=201, Percent_Identity=25.3731343283582, Blast_Score=76, Evalue=1e-13,
Organism=Escherichia coli, GI1788150, Length=341, Percent_Identity=24.633431085044, Blast_Score=108, Evalue=8e-25,
Organism=Drosophila melanogaster, GI24583414, Length=316, Percent_Identity=32.9113924050633, Blast_Score=179, Evalue=8e-45,
Organism=Drosophila melanogaster, GI221510989, Length=302, Percent_Identity=35.4304635761589, Blast_Score=169, Evalue=6e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001375
- InterPro:   IPR002470
- InterPro:   IPR004106 [H]

Pfam domain/function: PF00326 Peptidase_S9; PF02897 Peptidase_S9_N [H]

EC number: 3.4.21.83 [C]

Molecular weight: Translated: 73811; Mature: 73680

Theoretical pI: Translated: 4.31; Mature: 4.31

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.1 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.7 %Cys+Met (Translated Protein)
0.1 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDYTFLEDIDTPEALAWAEKWSGESVEKLKSPAKDALEARLLAALDTDDRIAYVSRRGE
CCCCCCHHCCCCCHHHHHHHHCCCCHHHHHHCHHHHHHHHHHHHEECCCCCEEHHHHCCH
KLYNFWRDAQHPRGVWRTTTLESYESDQPEWDVLIDVDALAEDEGENWVWKGAVVRSPEF
HHHHHHHCCCCCCCCEEEEEHHCCCCCCCCEEEEEEEHHHCCCCCCCEEEECEEEECCCH
DRALVKFSRGGADATVIREFDLATAAFVDDSPFELEEAKSDVTWVDLDTLLVGTDTGEGS
HHHHHHHHCCCCCEEEEEEECCEEEEEECCCCCCHHHHCCCCEEEEEEEEEEECCCCCCC
LTDSGYPARVLTWKRGTPLEQAELFFEGSRQDVATHAWRDSTPGFERTFVSRSLDFYNSE
CCCCCCCEEEEEECCCCCHHHHHHEECCCCHHHHHHCCCCCCCCHHHHHHHHHHHHCCCC
TSLETEGGLVKLDVPTDCDVIVKKQWIFVSPRTDFAGIPAGGLGVLLLKEFLEGGRDFQP
CCEECCCCEEEEECCCCCCEEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCC
VFTPTESTSLQGLATTKNFLVLTLLNNVSTEIVTVPLNDPTTEHEHIDLPEHVTAHVVAT
EECCCCCCCCCEEECCCCEEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCCEEEEEEE
SPLDGDEIWVQAASFTEAPTLLRAELPGALEAVKKAPLQFENAGQETRQHWATSADGTKI
CCCCCCCEEEEECCCCCCCHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCC
PYFITGAFEEEPQNTLVHAYGGFEVSLTPSHSPTRGIAWLEKGYYFVEANLRGGGEFGPE
CEEEECCCCCCCCCCEEEEECCEEEEECCCCCCCCCHHEEECCEEEEEEEECCCCCCCCH
WHSQATKLNRMKVWEDHRAVLADLVERGYATPEQIAIRGGSNGGLLTSGALTQYPEAFGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHEEEEECCCCCEEECCCHHHCHHHHCC
AVVQVPLADMLRYHTWSAGASWMAEYGNPDDPEERAVIEQYSPVQAVVGVEKRIYPPALV
CEEEECHHHHHHHHCCCCCHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCEEE
TTSTRDDRVHPAHARLFAQALLDAGQAVDYYENTEGGHAGAADNKQTAFVESLIYTWIEK
EECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
TLDQQGSI
HHHHCCCC
>Mature Secondary Structure 
TDYTFLEDIDTPEALAWAEKWSGESVEKLKSPAKDALEARLLAALDTDDRIAYVSRRGE
CCCCCHHCCCCCHHHHHHHHCCCCHHHHHHCHHHHHHHHHHHHEECCCCCEEHHHHCCH
KLYNFWRDAQHPRGVWRTTTLESYESDQPEWDVLIDVDALAEDEGENWVWKGAVVRSPEF
HHHHHHHCCCCCCCCEEEEEHHCCCCCCCCEEEEEEEHHHCCCCCCCEEEECEEEECCCH
DRALVKFSRGGADATVIREFDLATAAFVDDSPFELEEAKSDVTWVDLDTLLVGTDTGEGS
HHHHHHHHCCCCCEEEEEEECCEEEEEECCCCCCHHHHCCCCEEEEEEEEEEECCCCCCC
LTDSGYPARVLTWKRGTPLEQAELFFEGSRQDVATHAWRDSTPGFERTFVSRSLDFYNSE
CCCCCCCEEEEEECCCCCHHHHHHEECCCCHHHHHHCCCCCCCCHHHHHHHHHHHHCCCC
TSLETEGGLVKLDVPTDCDVIVKKQWIFVSPRTDFAGIPAGGLGVLLLKEFLEGGRDFQP
CCEECCCCEEEEECCCCCCEEEEEEEEEEECCCCCCCCCCCCHHHHHHHHHHHCCCCCCC
VFTPTESTSLQGLATTKNFLVLTLLNNVSTEIVTVPLNDPTTEHEHIDLPEHVTAHVVAT
EECCCCCCCCCEEECCCCEEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCCEEEEEEE
SPLDGDEIWVQAASFTEAPTLLRAELPGALEAVKKAPLQFENAGQETRQHWATSADGTKI
CCCCCCCEEEEECCCCCCCHHHHHCCCHHHHHHHHCCCCCCCCCHHHHHHHCCCCCCCCC
PYFITGAFEEEPQNTLVHAYGGFEVSLTPSHSPTRGIAWLEKGYYFVEANLRGGGEFGPE
CEEEECCCCCCCCCCEEEEECCEEEEECCCCCCCCCHHEEECCEEEEEEEECCCCCCCCH
WHSQATKLNRMKVWEDHRAVLADLVERGYATPEQIAIRGGSNGGLLTSGALTQYPEAFGA
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHEEEEECCCCCEEECCCHHHCHHHHCC
AVVQVPLADMLRYHTWSAGASWMAEYGNPDDPEERAVIEQYSPVQAVVGVEKRIYPPALV
CEEEECHHHHHHHHCCCCCHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHCCCCEEE
TTSTRDDRVHPAHARLFAQALLDAGQAVDYYENTEGGHAGAADNKQTAFVESLIYTWIEK
EECCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHH
TLDQQGSI
HHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Ca2+ [C]

Kcat value (1/min): 11820 [C]

Specific activity: NA

Km value (mM): 0.92 {benzoyl-Lys} 0.6 {N-benzoyl-Arg} 0.23 {tosyl-Arg} 0.5 {benzoyl-Arg} 0.48 {benzoyl-Arg} 0.25 {benzoyl-Arg} 0.33 {N-benzyloxycarbonyl-Lys} 0.31 {N-benzyloxycarbonyl-Lys} 80 {acetyl-tyrosine} 0.47 {tosyl-Lys-methyl} [C]

Substrates: Protein; H2O [C]

Specific reaction: Protein + H2O = hydrolyzed protein [C]

General reaction: Peptide bond hydrolysis [C]

Inhibitor: Antipain; Aromaticamidines; Benzamidine; Co2+; DFP; Fe2+; Hg2+; L-Arginine; Leupeptin sulfhydryl agents, trypsin inhibitors, 1, 10-phenanthroline; p-Aminobenzamidine; Tosyl -Leuchloromethyl ketone; Zn2+ [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]