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The map label for this gene is crp [H]

Identifier: 62389188

GI number: 62389188

Start: 306779

End: 307462

Strand: Reverse

Name: crp [H]

Synonym: cg0350

Alternate gene names: 62389188

Gene position: 307462-306779 (Counterclockwise)

Preceding gene: 62389189

Following gene: 62389182

Centisome position: 9.37

GC content: 59.36

Gene sequence:

>684_bases
GTGGAAGGTGTACAGGAGATCCTGTCGCGCGCCGGAATTTTTCAAGGCGTTGACCCAACGGCAGTCAATAACCTCATCCA
GGATATGGAGACCGTTCGCTTCCCACGCGGAGCAACCATCTTCGACGAGGGCGAGCCAGGTGACCGCCTTTACATCATCA
CCTCCGGCAAAGTGAAGCTTGCGCGCCACGCACCGGACGGCCGCGAAAACCTGCTGACCATCATGGGTCCTTCCGACATG
TTCGGTGAGCTCTCCATCTTCGACCCAGGCCCACGCACCTCCTCTGCAGTGTGTGTCACCGAAGTTCATGCAGCAACCAT
GAACTCTGACATGCTGCGCAACTGGGTAGCTGACCACCCAGCTATCGCTGAGCAGCTCCTGCGCGTTCTGGCTCGTCGTC
TGCGTCGCACCAACGCTTCCCTGGCTGACCTCATCTTCACCGACGTCCCAGGCCGCGTTGCTAAGACCCTTCTGCAGCTG
GCTAACCGCTTCGGCACCCAAGAAGCTGGCGCGCTGCGCGTGAACCACGACCTCACTCAGGAAGAAATCGCACAGCTCGT
CGGTGCTTCCCGTGAAACTGTGAATAAGGCTCTTGCAACGTTCGCACACCGTGGCTGGATTCGCCTCGAGGGCAAGTCCG
TCCTCATTGTGGACACCGAGCATTTGGCACGTCGCGCTCGATAA

Upstream 100 bases:

>100_bases
CAGTTAGGTGTCATCCGGATTTTATCTCAAACCCTAACACCCCAGGTGTTGCCACTCATCCGGACTCAAACAAGATGTGT
GCAGATGAAGGAGAAAAGCA

Downstream 100 bases:

>100_bases
TCACCAAAGCGCTAAAAAGCGCCTGATCAGTTTCATACTGATTAGGCGCTTTCTTTGTTGTTTAGTTAAAAGTGTTTAGT
TTGAGGCGGAAGCTTCCTGA

Product: CRP/FNR family transcriptional regulator

Products: NA

Alternate protein names: cAMP receptor protein; cAMP regulatory protein [H]

Number of amino acids: Translated: 227; Mature: 227

Protein sequence:

>227_residues
MEGVQEILSRAGIFQGVDPTAVNNLIQDMETVRFPRGATIFDEGEPGDRLYIITSGKVKLARHAPDGRENLLTIMGPSDM
FGELSIFDPGPRTSSAVCVTEVHAATMNSDMLRNWVADHPAIAEQLLRVLARRLRRTNASLADLIFTDVPGRVAKTLLQL
ANRFGTQEAGALRVNHDLTQEEIAQLVGASRETVNKALATFAHRGWIRLEGKSVLIVDTEHLARRAR

Sequences:

>Translated_227_residues
MEGVQEILSRAGIFQGVDPTAVNNLIQDMETVRFPRGATIFDEGEPGDRLYIITSGKVKLARHAPDGRENLLTIMGPSDM
FGELSIFDPGPRTSSAVCVTEVHAATMNSDMLRNWVADHPAIAEQLLRVLARRLRRTNASLADLIFTDVPGRVAKTLLQL
ANRFGTQEAGALRVNHDLTQEEIAQLVGASRETVNKALATFAHRGWIRLEGKSVLIVDTEHLARRAR
>Mature_227_residues
MEGVQEILSRAGIFQGVDPTAVNNLIQDMETVRFPRGATIFDEGEPGDRLYIITSGKVKLARHAPDGRENLLTIMGPSDM
FGELSIFDPGPRTSSAVCVTEVHAATMNSDMLRNWVADHPAIAEQLLRVLARRLRRTNASLADLIFTDVPGRVAKTLLQL
ANRFGTQEAGALRVNHDLTQEEIAQLVGASRETVNKALATFAHRGWIRLEGKSVLIVDTEHLARRAR

Specific function: This protein complexes with cyclic AMP and binds to specific DNA sites near the promoter to regulate the transcription of one or more genes involved in the induction of competence [H]

COG id: COG0664

COG function: function code T; cAMP-binding proteins - catabolite gene activator and regulatory subunit of cAMP-dependent protein kinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH crp-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789756, Length=204, Percent_Identity=30.3921568627451, Blast_Score=101, Evalue=5e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR018490
- InterPro:   IPR018488
- InterPro:   IPR000595
- InterPro:   IPR001808
- InterPro:   IPR012318
- InterPro:   IPR014710
- InterPro:   IPR018335
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00027 cNMP_binding; PF00325 Crp [H]

EC number: NA

Molecular weight: Translated: 24988; Mature: 24988

Theoretical pI: Translated: 7.17; Mature: 7.17

Prosite motif: PS50042 CNMP_BINDING_3 ; PS51063 HTH_CRP_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEGVQEILSRAGIFQGVDPTAVNNLIQDMETVRFPRGATIFDEGEPGDRLYIITSGKVKL
CCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCEECCCCCCCCEEEEEECCCEEE
ARHAPDGRENLLTIMGPSDMFGELSIFDPGPRTSSAVCVTEVHAATMNSDMLRNWVADHP
EECCCCCCCCEEEEECCCCCCCCEEEECCCCCCCCEEEHHHHHHHHHCHHHHHHHHCCCH
AIAEQLLRVLARRLRRTNASLADLIFTDVPGRVAKTLLQLANRFGTQEAGALRVNHDLTQ
HHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCH
EEIAQLVGASRETVNKALATFAHRGWIRLEGKSVLIVDTEHLARRAR
HHHHHHHCCCHHHHHHHHHHHHHCCEEEECCCEEEEEEHHHHHHHCC
>Mature Secondary Structure
MEGVQEILSRAGIFQGVDPTAVNNLIQDMETVRFPRGATIFDEGEPGDRLYIITSGKVKL
CCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCCCEECCCCCCCCEEEEEECCCEEE
ARHAPDGRENLLTIMGPSDMFGELSIFDPGPRTSSAVCVTEVHAATMNSDMLRNWVADHP
EECCCCCCCCEEEEECCCCCCCCEEEECCCCCCCCEEEHHHHHHHHHCHHHHHHHHCCCH
AIAEQLLRVLARRLRRTNASLADLIFTDVPGRVAKTLLQLANRFGTQEAGALRVNHDLTQ
HHHHHHHHHHHHHHHHCCCHHHHHHHHCCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCH
EEIAQLVGASRETVNKALATFAHRGWIRLEGKSVLIVDTEHLARRAR
HHHHHHHCCCHHHHHHHHHHHHHCCEEEECCCEEEEEEHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1542653; 7542800 [H]