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The map label for this gene is ccpA [H]

Identifier: 62389074

GI number: 62389074

Start: 188745

End: 189848

Strand: Reverse

Name: ccpA [H]

Synonym: cg0221

Alternate gene names: 62389074

Gene position: 189848-188745 (Counterclockwise)

Preceding gene: 62389076

Following gene: 62389073

Centisome position: 5.78

GC content: 52.45

Gene sequence:

>1104_bases
GTGCTACCGAAACCCCTCGTGTTTAAATCTAGAAGTTTAAAGGGTGAAAACAGTCCATTACTTAAGCACCAATCTGCCAT
AATTTTTACCCCAACGCATAGGCTTAACGGTGTGAATGTGAAGTTAACTGACGCCGCCCGTGAAGCTGGAGTAGGTTACG
GTACTGCTTCTCGCGCAATTTCTGGACGAGGTTCCGTTGATGCAGCAACCCGTGACAAAGTACTCGCCGCCGCCGAGAAA
CTTGGGTACCGAACCAACGCCATGGCTCGTGCACTTAGGGAAAACAAGACCCGCACCGTTGGCCTGATCGTTCCCGGCAT
TATCAATAAGTTCTACACCGAATCCGCCACTGTCCTCCAAGATGAATTAGACAAATCCGGATACCAACTAGTTGTTTCCA
CAACTGGAAACGACGCAGAAAAGGAACGTCGAGCTATCGAATCCATGCTCAACCGCCAGGTAGATGCAGTGGTGCACGCT
CCAGTTAATCCCCAAGCGAAGTTTCCAAAGGGCTTCAAAGTGGTCGAGCTTAATCGTCGTAGCGATCTCAACCGACCTAC
TGTGACCAGCGATGATGCCACTGGTTTGAAGGAACTTGCTCTTCATATTTTGGATCAGGGATACCGAGATATAGGTATCA
TTGTCGGTCCTGCTGAGCTCAGCACCGCCCGAGACCGCAAAGCCGGATTCATCAACGCCCTCGAAACCGAAGCCACACAA
CGCGGAATCCGCGAAGAACTACGATTCCGGGTAGTTCACTCCCGCTACTCCCCCACCGGCGGTTATGAAGCATTCGCAGA
ATTCCGCAATGATCTCCCTCAAATCGTGGTGCCCCTGAGCACGCAATTAACTCTAGGAGTTCTCAAAGCAACCCAAGAAA
ACGGCATAAAAATATCGGATGACCTGGCACTTGCTTGTTACGGCGTCGCCGAATGGCTCGCAGTGTGGGGCCCTGGCATC
ACCGTTTTCGCACCAGACCTCCCAGCCATGGGCGCCGCAGCTGCCACGCAGGTTTTAACGCTTCTCGACGCCGCCCCACT
CCCCGAAAACCACTTAAGCATTCCGGGGCAGCTCATTGTCCGTGGGACAACTCCAAAGGTTTAA

Upstream 100 bases:

>100_bases
CGTGGAGTCTTCTAAATTTTCGAGTCATAGTAAAGTTCGCTTAATCGCCATCAGAGTAGTAGCAGTGGGTATTTGCAAGC
CTTTGACAAGTTACTAAACC

Downstream 100 bases:

>100_bases
AGGTAGAGGCGCACAATAATGAAAATTGAAATTTTCAGCCCAGAGGCCATGCAATCCGCTGAGTTCGCTCACCTTTTGTG
GCTTGCTTCCGGCTATTCTG

Product: LacI family transcriptional regulator

Products: NA

Alternate protein names: Glucose-resistance amylase regulator [H]

Number of amino acids: Translated: 367; Mature: 367

Protein sequence:

>367_residues
MLPKPLVFKSRSLKGENSPLLKHQSAIIFTPTHRLNGVNVKLTDAAREAGVGYGTASRAISGRGSVDAATRDKVLAAAEK
LGYRTNAMARALRENKTRTVGLIVPGIINKFYTESATVLQDELDKSGYQLVVSTTGNDAEKERRAIESMLNRQVDAVVHA
PVNPQAKFPKGFKVVELNRRSDLNRPTVTSDDATGLKELALHILDQGYRDIGIIVGPAELSTARDRKAGFINALETEATQ
RGIREELRFRVVHSRYSPTGGYEAFAEFRNDLPQIVVPLSTQLTLGVLKATQENGIKISDDLALACYGVAEWLAVWGPGI
TVFAPDLPAMGAAAATQVLTLLDAAPLPENHLSIPGQLIVRGTTPKV

Sequences:

>Translated_367_residues
MLPKPLVFKSRSLKGENSPLLKHQSAIIFTPTHRLNGVNVKLTDAAREAGVGYGTASRAISGRGSVDAATRDKVLAAAEK
LGYRTNAMARALRENKTRTVGLIVPGIINKFYTESATVLQDELDKSGYQLVVSTTGNDAEKERRAIESMLNRQVDAVVHA
PVNPQAKFPKGFKVVELNRRSDLNRPTVTSDDATGLKELALHILDQGYRDIGIIVGPAELSTARDRKAGFINALETEATQ
RGIREELRFRVVHSRYSPTGGYEAFAEFRNDLPQIVVPLSTQLTLGVLKATQENGIKISDDLALACYGVAEWLAVWGPGI
TVFAPDLPAMGAAAATQVLTLLDAAPLPENHLSIPGQLIVRGTTPKV
>Mature_367_residues
MLPKPLVFKSRSLKGENSPLLKHQSAIIFTPTHRLNGVNVKLTDAAREAGVGYGTASRAISGRGSVDAATRDKVLAAAEK
LGYRTNAMARALRENKTRTVGLIVPGIINKFYTESATVLQDELDKSGYQLVVSTTGNDAEKERRAIESMLNRQVDAVVHA
PVNPQAKFPKGFKVVELNRRSDLNRPTVTSDDATGLKELALHILDQGYRDIGIIVGPAELSTARDRKAGFINALETEATQ
RGIREELRFRVVHSRYSPTGGYEAFAEFRNDLPQIVVPLSTQLTLGVLKATQENGIKISDDLALACYGVAEWLAVWGPGI
TVFAPDLPAMGAAAATQVLTLLDAAPLPENHLSIPGQLIVRGTTPKV

Specific function: Transcriptional regulator involved in both the repression of carbohydrate utilization genes such as the alpha- amylase (AmyE) and the acetyl-coenzyme A synthetase (AcsA); and in the positive regulation of genes involved in excretion of excess carbon such

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=334, Percent_Identity=25.1497005988024, Blast_Score=112, Evalue=3e-26,
Organism=Escherichia coli, GI1786268, Length=295, Percent_Identity=31.5254237288136, Blast_Score=107, Evalue=2e-24,
Organism=Escherichia coli, GI1789202, Length=316, Percent_Identity=28.7974683544304, Blast_Score=98, Evalue=1e-21,
Organism=Escherichia coli, GI1789068, Length=289, Percent_Identity=27.681660899654, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1790194, Length=331, Percent_Identity=24.1691842900302, Blast_Score=95, Evalue=7e-21,
Organism=Escherichia coli, GI1787948, Length=309, Percent_Identity=24.2718446601942, Blast_Score=93, Evalue=2e-20,
Organism=Escherichia coli, GI1786540, Length=338, Percent_Identity=23.9644970414201, Blast_Score=90, Evalue=3e-19,
Organism=Escherichia coli, GI48994940, Length=306, Percent_Identity=24.8366013071895, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1788474, Length=314, Percent_Identity=27.7070063694268, Blast_Score=79, Evalue=3e-16,
Organism=Escherichia coli, GI1787906, Length=203, Percent_Identity=29.5566502463054, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI1787580, Length=327, Percent_Identity=21.7125382262997, Blast_Score=65, Evalue=6e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006377
- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 39651; Mature: 39651

Theoretical pI: Translated: 9.76; Mature: 9.76

Prosite motif: PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLPKPLVFKSRSLKGENSPLLKHQSAIIFTPTHRLNGVNVKLTDAAREAGVGYGTASRAI
CCCCCCEECCCCCCCCCCCCEECCCEEEEECCCCCCCCEEEEECHHHHCCCCCCCHHHHC
SGRGSVDAATRDKVLAAAEKLGYRTNAMARALRENKTRTVGLIVPGIINKFYTESATVLQ
CCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEECHHHHHHHHCCCHHHHH
DELDKSGYQLVVSTTGNDAEKERRAIESMLNRQVDAVVHAPVNPQAKFPKGFKVVELNRR
HHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCEEEEECCC
SDLNRPTVTSDDATGLKELALHILDQGYRDIGIIVGPAELSTARDRKAGFINALETEATQ
CCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHCCHHHHHHHHHHH
RGIREELRFRVVHSRYSPTGGYEAFAEFRNDLPQIVVPLSTQLTLGVLKATQENGIKISD
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCCCEECC
DLALACYGVAEWLAVWGPGITVFAPDLPAMGAAAATQVLTLLDAAPLPENHLSIPGQLIV
CHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEE
RGTTPKV
ECCCCCC
>Mature Secondary Structure
MLPKPLVFKSRSLKGENSPLLKHQSAIIFTPTHRLNGVNVKLTDAAREAGVGYGTASRAI
CCCCCCEECCCCCCCCCCCCEECCCEEEEECCCCCCCCEEEEECHHHHCCCCCCCHHHHC
SGRGSVDAATRDKVLAAAEKLGYRTNAMARALRENKTRTVGLIVPGIINKFYTESATVLQ
CCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCEEEEECHHHHHHHHCCCHHHHH
DELDKSGYQLVVSTTGNDAEKERRAIESMLNRQVDAVVHAPVNPQAKFPKGFKVVELNRR
HHHCCCCCEEEEEECCCCHHHHHHHHHHHHHHHHHEEEECCCCCCCCCCCCCEEEEECCC
SDLNRPTVTSDDATGLKELALHILDQGYRDIGIIVGPAELSTARDRKAGFINALETEATQ
CCCCCCCCCCCCCCCHHHHHHHHHHCCCCEEEEEECCHHHHHHHHHHCCHHHHHHHHHHH
RGIREELRFRVVHSRYSPTGGYEAFAEFRNDLPQIVVPLSTQLTLGVLKATQENGIKISD
HHHHHHHHHHHHHHCCCCCCCHHHHHHHHCCCCEEEECCCCHHHHHHHHHHHCCCCEECC
DLALACYGVAEWLAVWGPGITVFAPDLPAMGAAAATQVLTLLDAAPLPENHLSIPGQLIV
CHHHHHHHHHHHHHHHCCCCEEECCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEE
RGTTPKV
ECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1904524; 9387221; 9384377 [H]