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The map label for this gene is pstS1 [H]

Identifier: 59713738

GI number: 59713738

Start: 638920

End: 639732

Strand: Reverse

Name: pstS1 [H]

Synonym: VF_A0555

Alternate gene names: 59713738

Gene position: 639732-638920 (Counterclockwise)

Preceding gene: 59713739

Following gene: 59713737

Centisome position: 48.09

GC content: 36.9

Gene sequence:

>813_bases
ATGTTCAAAGCGTTTAGTTACACCACCATATTTACAGCGTTACTTTTTAGCTCCTCAACTTTTGCTGCAAATACAGTAAT
TGCAGGTTCTACATCTGTTTCTACACTATTGGAACCCTTTATTGATGAATACAATAAAGACAATAAACCACTTGTTGATT
TGCAAAGTATTGGTTCAAGTGCAGGCATTAAACTACTGGTTGATGACACAGTAAATCTTGCTATCAGCTCTCGTGAGTTA
ACAGACAAAGAAAAAGAGACATTAAGTTCAACTCTTATCGCTTATGATGCCATCGCAATTGTTGTCCACCCTGACAACCC
AATTACTGAATTATCTACAGAGAACATCTGGAAAATCTATCACGGTGATATTAAAAACTGGAAAGAATTGGGTGGCACCG
ATCGTAAGATCTCTGTAATTTCACGTGAAATTGCTTCAGGCTCTCGCTTCTCGTTTGAAAAACACTTTGGTTTAACACAA
GAAATCAACTCATTCACTGTATCAGATATTAGCGATAACGCTCTTGTTGTTAATGGTACGGGTATGGTTAAGAGCATTGT
TTCACGCAACCCTTATGCTATTGGCTATGTATCTGCTGGAACACTTGGTGATCTTGCTCAACTCCGTGTACTAAAAGTAG
ATAACGTACTACCAAGCCAAGAAGCTATTATGGAAAATAAATATTCTCTTTCCCACCCTTTCTTATTTGTATACAAGAAC
CAAACAGTATCTAAAGAATCGACAGAGTTTATGAATTACATTGCTTCACCAGAAGCAACTAAAACCATCCAATCACTTGG
TTATATCAAGTAA

Upstream 100 bases:

>100_bases
TCACCCCAAAAGAATTTCGTGATTCACTTCAAGGTTAATTTGTCACAATGGTGTAACACTTCCCGACTATGATGCTTTTT
TTTCATTATGGATTTAGGTC

Downstream 100 bases:

>100_bases
CTATTGAAAATAATGGTCGAATAACATATTGTTTACTCGATTTTAATTTCAAGGATGTCACATGTTAACCGTAAATAGCT
ATTTTGAAGATAAAGTAAAA

Product: phosphate-binding protein

Products: ADP; phosphate [Cytoplasm]; phosphate [Cytoplasm] [C]

Alternate protein names: PBP 1 [H]

Number of amino acids: Translated: 270; Mature: 270

Protein sequence:

>270_residues
MFKAFSYTTIFTALLFSSSTFAANTVIAGSTSVSTLLEPFIDEYNKDNKPLVDLQSIGSSAGIKLLVDDTVNLAISSREL
TDKEKETLSSTLIAYDAIAIVVHPDNPITELSTENIWKIYHGDIKNWKELGGTDRKISVISREIASGSRFSFEKHFGLTQ
EINSFTVSDISDNALVVNGTGMVKSIVSRNPYAIGYVSAGTLGDLAQLRVLKVDNVLPSQEAIMENKYSLSHPFLFVYKN
QTVSKESTEFMNYIASPEATKTIQSLGYIK

Sequences:

>Translated_270_residues
MFKAFSYTTIFTALLFSSSTFAANTVIAGSTSVSTLLEPFIDEYNKDNKPLVDLQSIGSSAGIKLLVDDTVNLAISSREL
TDKEKETLSSTLIAYDAIAIVVHPDNPITELSTENIWKIYHGDIKNWKELGGTDRKISVISREIASGSRFSFEKHFGLTQ
EINSFTVSDISDNALVVNGTGMVKSIVSRNPYAIGYVSAGTLGDLAQLRVLKVDNVLPSQEAIMENKYSLSHPFLFVYKN
QTVSKESTEFMNYIASPEATKTIQSLGYIK
>Mature_270_residues
MFKAFSYTTIFTALLFSSSTFAANTVIAGSTSVSTLLEPFIDEYNKDNKPLVDLQSIGSSAGIKLLVDDTVNLAISSREL
TDKEKETLSSTLIAYDAIAIVVHPDNPITELSTENIWKIYHGDIKNWKELGGTDRKISVISREIASGSRFSFEKHFGLTQ
EINSFTVSDISDNALVVNGTGMVKSIVSRNPYAIGYVSAGTLGDLAQLRVLKVDNVLPSQEAIMENKYSLSHPFLFVYKN
QTVSKESTEFMNYIASPEATKTIQSLGYIK

Specific function: Part of the ABC transporter complex pstSACB involved in phosphate import [H]

COG id: COG0226

COG function: function code P; ABC-type phosphate transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pstS family [H]

Homologues:

None

Paralogues:

None

Copy number: 20 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011862
- InterPro:   IPR006059 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 29679; Mature: 29679

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFKAFSYTTIFTALLFSSSTFAANTVIAGSTSVSTLLEPFIDEYNKDNKPLVDLQSIGSS
CCCCHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCCCEEEHHHCCCC
AGIKLLVDDTVNLAISSRELTDKEKETLSSTLIAYDAIAIVVHPDNPITELSTENIWKIY
CCEEEEEECCEEEEEECCHHCHHHHHHHHHHHEEEEEEEEEECCCCCCCCCCCCCEEEEE
HGDIKNWKELGGTDRKISVISREIASGSRFSFEKHFGLTQEINSFTVSDISDNALVVNGT
ECCHHHHHHCCCCCCCHHHHHHHHHCCCCCCHHHHCCCHHHHCCEEEECCCCCEEEEECC
GMVKSIVSRNPYAIGYVSAGTLGDLAQLRVLKVDNVLPSQEAIMENKYSLSHPFLFVYKN
HHHHHHHCCCCEEEEEECCCCCHHHHHEEEEEECCCCCCHHHHHHCCCCCCCCEEEEECC
QTVSKESTEFMNYIASPEATKTIQSLGYIK
CCCCHHHHHHHHHHCCCHHHHHHHHHCCCC
>Mature Secondary Structure
MFKAFSYTTIFTALLFSSSTFAANTVIAGSTSVSTLLEPFIDEYNKDNKPLVDLQSIGSS
CCCCHHHHHHHHHHHHCCCCCCCCEEEECCCHHHHHHHHHHHHHCCCCCCEEEHHHCCCC
AGIKLLVDDTVNLAISSRELTDKEKETLSSTLIAYDAIAIVVHPDNPITELSTENIWKIY
CCEEEEEECCEEEEEECCHHCHHHHHHHHHHHEEEEEEEEEECCCCCCCCCCCCCEEEEE
HGDIKNWKELGGTDRKISVISREIASGSRFSFEKHFGLTQEINSFTVSDISDNALVVNGT
ECCHHHHHHCCCCCCCHHHHHHHHHCCCCCCHHHHCCCHHHHCCEEEECCCCCEEEEECC
GMVKSIVSRNPYAIGYVSAGTLGDLAQLRVLKVDNVLPSQEAIMENKYSLSHPFLFVYKN
HHHHHHHCCCCEEEEEECCCCCHHHHHEEEEEECCCCCCHHHHHHCCCCCCCCEEEEECC
QTVSKESTEFMNYIASPEATKTIQSLGYIK
CCCCHHHHHHHHHHCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; phosphate [Periplasm]; H2O [C]

Specific reaction: ATP + phosphate [Periplasm] + H2O = ADP + phosphate [Cytoplasm] + phosphate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12354221 [H]