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The map label for this gene is rnt [H]

Identifier: 59711532

GI number: 59711532

Start: 1020833

End: 1021501

Strand: Reverse

Name: rnt [H]

Synonym: VF_0925

Alternate gene names: 59711532

Gene position: 1021501-1020833 (Counterclockwise)

Preceding gene: 59711534

Following gene: 59711531

Centisome position: 35.25

GC content: 38.57

Gene sequence:

>669_bases
ATGATGACTGAAGTAGAACAACACAATTTATTAAAAAACCGTTTTCGTGGCTATTTTCCTGTAGTTATTGATGTTGAAAC
AGCAGGTTTTAATGCTAAAACTGATGCATTACTTGAGATTTGTGCTGTTACTCTCGCTATGGATGAGCAAGGTAATTTAA
AACCTGCTACCACCATCCACTTCCATGTCACTCCTTTTGAAGGTGCTAATATTGAAAAAGAAGCACTTGAATTTAATGGT
ATACGTGACCCATTTAGCCCATTACGTGGTGCTGTCACTGAAGACGTTGCATTAAAAGAAATCTTCAAACTTGTCCGTAA
AGAACAAAAACAACACGATTGCCAACGTGCAATAATGGTTGCTCATAACGCTCATTTTGACCATAGCTTTGTTATGGAAG
CAGCGGCACGAACAAAATTAAAACGCATTCCTTTCCATCCATTTTGCACCTTTGATACAGCGGCAATCAGTGGCCTAGCA
CTTGGTCAAACTGTATTGGCAAAGGCATGTAAAACGGCAGGAATGGATTTTGATGGTAAAGAAGCTCATTCAGCTCTTTA
TGATACAGAAAAGACGGCAGAACTATTCTGTCGTATTGTGAATAAATGGAAAGATCTTGGTGGCTGGCCTGTAATGCCAG
AACCAGAAAACGATACTAATAATAATTAA

Upstream 100 bases:

>100_bases
CAGTTAGATACTAATAAATATATCTCTATCACACCTTATTTACAGTTTTTATGCTACGGATAATCTGCAATAATGGTCAT
AACATTTTCTTACGTACCGA

Downstream 100 bases:

>100_bases
TTGGCTAATTGCTAACTACATAATAATTTACAGGGAACTAAACACAATGAATCCAATCATTCTTTCTGTTTCAGTTATGC
TTATCCTTGCTTTGCTTAGA

Product: ribonuclease T

Products: NA

Alternate protein names: Exoribonuclease T; RNase T [H]

Number of amino acids: Translated: 222; Mature: 222

Protein sequence:

>222_residues
MMTEVEQHNLLKNRFRGYFPVVIDVETAGFNAKTDALLEICAVTLAMDEQGNLKPATTIHFHVTPFEGANIEKEALEFNG
IRDPFSPLRGAVTEDVALKEIFKLVRKEQKQHDCQRAIMVAHNAHFDHSFVMEAAARTKLKRIPFHPFCTFDTAAISGLA
LGQTVLAKACKTAGMDFDGKEAHSALYDTEKTAELFCRIVNKWKDLGGWPVMPEPENDTNNN

Sequences:

>Translated_222_residues
MMTEVEQHNLLKNRFRGYFPVVIDVETAGFNAKTDALLEICAVTLAMDEQGNLKPATTIHFHVTPFEGANIEKEALEFNG
IRDPFSPLRGAVTEDVALKEIFKLVRKEQKQHDCQRAIMVAHNAHFDHSFVMEAAARTKLKRIPFHPFCTFDTAAISGLA
LGQTVLAKACKTAGMDFDGKEAHSALYDTEKTAELFCRIVNKWKDLGGWPVMPEPENDTNNN
>Mature_222_residues
MMTEVEQHNLLKNRFRGYFPVVIDVETAGFNAKTDALLEICAVTLAMDEQGNLKPATTIHFHVTPFEGANIEKEALEFNG
IRDPFSPLRGAVTEDVALKEIFKLVRKEQKQHDCQRAIMVAHNAHFDHSFVMEAAARTKLKRIPFHPFCTFDTAAISGLA
LGQTVLAKACKTAGMDFDGKEAHSALYDTEKTAELFCRIVNKWKDLGGWPVMPEPENDTNNN

Specific function: Responsible for the end-turnover of tRNA:specifically removes the terminal AMP residue from uncharged tRNA (tRNA-C-C-A). Also appears to be involved in tRNA biosynthesis [H]

COG id: COG0847

COG function: function code L; DNA polymerase III, epsilon subunit and related 3'-5' exonucleases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 exonuclease domain [H]

Homologues:

Organism=Escherichia coli, GI1787941, Length=215, Percent_Identity=70.2325581395349, Blast_Score=305, Evalue=1e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006055
- InterPro:   IPR013520
- InterPro:   IPR005987
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00929 Exonuc_X-T [H]

EC number: 3.1.13.- [C]

Molecular weight: Translated: 24850; Mature: 24850

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.4 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMTEVEQHNLLKNRFRGYFPVVIDVETAGFNAKTDALLEICAVTLAMDEQGNLKPATTIH
CCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEE
FHVTPFEGANIEKEALEFNGIRDPFSPLRGAVTEDVALKEIFKLVRKEQKQHDCQRAIMV
EEECCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AHNAHFDHSFVMEAAARTKLKRIPFHPFCTFDTAAISGLALGQTVLAKACKTAGMDFDGK
HHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
EAHSALYDTEKTAELFCRIVNKWKDLGGWPVMPEPENDTNNN
HHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MMTEVEQHNLLKNRFRGYFPVVIDVETAGFNAKTDALLEICAVTLAMDEQGNLKPATTIH
CCCCCHHHHHHHHHCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHCCCCCCCCCCEEEE
FHVTPFEGANIEKEALEFNGIRDPFSPLRGAVTEDVALKEIFKLVRKEQKQHDCQRAIMV
EEECCCCCCCCHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AHNAHFDHSFVMEAAARTKLKRIPFHPFCTFDTAAISGLALGQTVLAKACKTAGMDFDGK
HHCCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCH
EAHSALYDTEKTAELFCRIVNKWKDLGGWPVMPEPENDTNNN
HHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on ester bonds [C]

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA