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The map label for this gene is metK [H]

Identifier: 58617671

GI number: 58617671

Start: 1490123

End: 1491328

Strand: Reverse

Name: metK [H]

Synonym: ERGA_CDS_09440

Alternate gene names: 58617671

Gene position: 1491328-1490123 (Counterclockwise)

Preceding gene: 58617673

Following gene: 58617666

Centisome position: 99.43

GC content: 30.1

Gene sequence:

>1206_bases
ATGTTTTATTATAAAAACTGTCCATCTAGTTACATTATAACTAGTGAGTCTGTTTCTAGTGGGCATCCTGATAAAATTGC
TGATCAAATATCTGATGCAATACTAGATTATTATATATCGTTAAATCCGTTTGTACATACTGCTATTGAAACTTTAGTTA
CAAAAAATAAGGTTTTTATTGCTGGTGAAATTTGTGGAGTTAATGTTGATAATTCTATTATTCAATCTATAGCTAGGAGT
GTTATAAAGGAAATAGGTTATGAGCAAGATGGTTTTCATTGGGAGAATGTTGATATTGAAATATTAATACATGAACAATC
TCCTGACATAATTGTTGGAGTTAATTCTGGTGAAAATCAAGGTGCTGGTGATCAAGGTATAGTTTATGGATATGCTATAA
ATGAGACAGAAACTCTTATGCCTGCTTCAATTTTTTATTCTCATCTGATTTTAAGAAATATTATGGATGCAGTAAAAAGT
TGTGAAATACCAAAGCTTGGACCAGATGCCAAAACACAAATTACGTTAGTATATGAAAATAATATACCAGTTAAAGTATC
TAATGTTATACTTTCTATACAGCATCCTGAATATATTACTCAAAATCAAATTAGGGAAATTGTATATCCTTATGTCAGTA
AATCCTTACCTAAGGGATGGATGTGTTCTGATAGTAATTTTTTAGTTAATACAACAGGGAGATTTGTAATAGGTGGTCCA
GTAGGAGATTGTGGTTTAACAGGTCGTAAGATTATGATAGATACTTATGGTGGACATGTACCTCATGGTGGAGGGGCATT
TTCTGGAAAAGATCCAAGTAAAGTAGATAGATCTGCTGCTTATATGGCTCGTTATTTAGCTAAAAACATAGTTTCTGCTA
AGTTAGCTACTAAATGTTTAGTGCAAATATCATATGCTATTGGAATTTCATCTCCTACTTCTTTTTATGTGAATACTTTT
GGTACAGGTATAATTGATGATAATGAAATTCAGAAATTTATAATGGATAACATAGATTTGTCTCCCTATGGAATATGTAA
GCATTTATTATTATTTAATCCTATTTATAAGATTACATCTTCTTATGGGCATTTTGGTAGAATTCCTAGTCTAAATGGAA
GTTTTTCATGGGAAAAAGAAGATCTTGCTTTGCAAATAAATCGTAAATTTCATTTTGATAAAAATATATCTCATGCTTAT
ATATAG

Upstream 100 bases:

>100_bases
ATCTTTTATAGTAAGTTTGATATTATTAAGTGATTGATTATATATTGTTTATTAATTTCATATTTGGTACTATAAAAGTC
TTTAGGTTTATGTTTTTAAC

Downstream 100 bases:

>100_bases
CTTATTTTTTGTAAGTATATAATTATTGGTATTGTTATTATTTTAGAGTTAGCTTTCTTTATATAGAAAAAGGTAAAATG
CTTTATTTTAAATGTTGGTT

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 401; Mature: 401

Protein sequence:

>401_residues
MFYYKNCPSSYIITSESVSSGHPDKIADQISDAILDYYISLNPFVHTAIETLVTKNKVFIAGEICGVNVDNSIIQSIARS
VIKEIGYEQDGFHWENVDIEILIHEQSPDIIVGVNSGENQGAGDQGIVYGYAINETETLMPASIFYSHLILRNIMDAVKS
CEIPKLGPDAKTQITLVYENNIPVKVSNVILSIQHPEYITQNQIREIVYPYVSKSLPKGWMCSDSNFLVNTTGRFVIGGP
VGDCGLTGRKIMIDTYGGHVPHGGGAFSGKDPSKVDRSAAYMARYLAKNIVSAKLATKCLVQISYAIGISSPTSFYVNTF
GTGIIDDNEIQKFIMDNIDLSPYGICKHLLLFNPIYKITSSYGHFGRIPSLNGSFSWEKEDLALQINRKFHFDKNISHAY
I

Sequences:

>Translated_401_residues
MFYYKNCPSSYIITSESVSSGHPDKIADQISDAILDYYISLNPFVHTAIETLVTKNKVFIAGEICGVNVDNSIIQSIARS
VIKEIGYEQDGFHWENVDIEILIHEQSPDIIVGVNSGENQGAGDQGIVYGYAINETETLMPASIFYSHLILRNIMDAVKS
CEIPKLGPDAKTQITLVYENNIPVKVSNVILSIQHPEYITQNQIREIVYPYVSKSLPKGWMCSDSNFLVNTTGRFVIGGP
VGDCGLTGRKIMIDTYGGHVPHGGGAFSGKDPSKVDRSAAYMARYLAKNIVSAKLATKCLVQISYAIGISSPTSFYVNTF
GTGIIDDNEIQKFIMDNIDLSPYGICKHLLLFNPIYKITSSYGHFGRIPSLNGSFSWEKEDLALQINRKFHFDKNISHAY
I
>Mature_401_residues
MFYYKNCPSSYIITSESVSSGHPDKIADQISDAILDYYISLNPFVHTAIETLVTKNKVFIAGEICGVNVDNSIIQSIARS
VIKEIGYEQDGFHWENVDIEILIHEQSPDIIVGVNSGENQGAGDQGIVYGYAINETETLMPASIFYSHLILRNIMDAVKS
CEIPKLGPDAKTQITLVYENNIPVKVSNVILSIQHPEYITQNQIREIVYPYVSKSLPKGWMCSDSNFLVNTTGRFVIGGP
VGDCGLTGRKIMIDTYGGHVPHGGGAFSGKDPSKVDRSAAYMARYLAKNIVSAKLATKCLVQISYAIGISSPTSFYVNTF
GTGIIDDNEIQKFIMDNIDLSPYGICKHLLLFNPIYKITSSYGHFGRIPSLNGSFSWEKEDLALQINRKFHFDKNISHAY
I

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI5174529, Length=380, Percent_Identity=42.6315789473684, Blast_Score=295, Evalue=5e-80,
Organism=Homo sapiens, GI4557737, Length=378, Percent_Identity=43.1216931216931, Blast_Score=293, Evalue=2e-79,
Organism=Escherichia coli, GI1789311, Length=381, Percent_Identity=50.1312335958005, Blast_Score=369, Evalue=1e-103,
Organism=Caenorhabditis elegans, GI17538494, Length=379, Percent_Identity=43.0079155672823, Blast_Score=300, Evalue=7e-82,
Organism=Caenorhabditis elegans, GI17551082, Length=389, Percent_Identity=43.4447300771208, Blast_Score=300, Evalue=1e-81,
Organism=Caenorhabditis elegans, GI17538498, Length=379, Percent_Identity=42.7440633245383, Blast_Score=299, Evalue=1e-81,
Organism=Caenorhabditis elegans, GI25145633, Length=398, Percent_Identity=41.9597989949749, Blast_Score=298, Evalue=2e-81,
Organism=Caenorhabditis elegans, GI32565905, Length=377, Percent_Identity=39.7877984084881, Blast_Score=265, Evalue=2e-71,
Organism=Caenorhabditis elegans, GI32565911, Length=328, Percent_Identity=41.7682926829268, Blast_Score=251, Evalue=7e-67,
Organism=Caenorhabditis elegans, GI25145635, Length=345, Percent_Identity=41.1594202898551, Blast_Score=250, Evalue=7e-67,
Organism=Caenorhabditis elegans, GI32565903, Length=299, Percent_Identity=43.1438127090301, Blast_Score=239, Evalue=2e-63,
Organism=Caenorhabditis elegans, GI32565909, Length=87, Percent_Identity=41.3793103448276, Blast_Score=72, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6320710, Length=381, Percent_Identity=44.6194225721785, Blast_Score=302, Evalue=6e-83,
Organism=Saccharomyces cerevisiae, GI6323209, Length=381, Percent_Identity=43.3070866141732, Blast_Score=291, Evalue=1e-79,
Organism=Drosophila melanogaster, GI24580527, Length=381, Percent_Identity=43.8320209973753, Blast_Score=285, Evalue=5e-77,
Organism=Drosophila melanogaster, GI24580525, Length=381, Percent_Identity=43.8320209973753, Blast_Score=285, Evalue=5e-77,
Organism=Drosophila melanogaster, GI45552159, Length=381, Percent_Identity=43.5695538057743, Blast_Score=281, Evalue=7e-76,
Organism=Drosophila melanogaster, GI24580529, Length=381, Percent_Identity=43.5695538057743, Blast_Score=281, Evalue=7e-76,
Organism=Drosophila melanogaster, GI24580537, Length=381, Percent_Identity=43.5695538057743, Blast_Score=281, Evalue=7e-76,
Organism=Drosophila melanogaster, GI24580533, Length=381, Percent_Identity=43.5695538057743, Blast_Score=281, Evalue=7e-76,
Organism=Drosophila melanogaster, GI24580535, Length=381, Percent_Identity=43.5695538057743, Blast_Score=281, Evalue=7e-76,
Organism=Drosophila melanogaster, GI24580531, Length=381, Percent_Identity=43.5695538057743, Blast_Score=281, Evalue=7e-76,
Organism=Drosophila melanogaster, GI24580541, Length=366, Percent_Identity=43.4426229508197, Blast_Score=266, Evalue=1e-71,
Organism=Drosophila melanogaster, GI24580539, Length=130, Percent_Identity=37.6923076923077, Blast_Score=91, Evalue=1e-18,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 44410; Mature: 44410

Theoretical pI: Translated: 6.23; Mature: 6.23

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFYYKNCPSSYIITSESVSSGHPDKIADQISDAILDYYISLNPFVHTAIETLVTKNKVFI
CCEECCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEE
AGEICGVNVDNSIIQSIARSVIKEIGYEQDGFHWENVDIEILIHEQSPDIIVGVNSGENQ
EEEEEECCCCHHHHHHHHHHHHHHCCCCCCCEEECCCEEEEEEECCCCCEEEEECCCCCC
GAGDQGIVYGYAINETETLMPASIFYSHLILRNIMDAVKSCEIPKLGPDAKTQITLVYEN
CCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEC
NIPVKVSNVILSIQHPEYITQNQIREIVYPYVSKSLPKGWMCSDSNFLVNTTGRFVIGGP
CCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEECCCCEEECCC
VGDCGLTGRKIMIDTYGGHVPHGGGAFSGKDPSKVDRSAAYMARYLAKNIVSAKLATKCL
CCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VQISYAIGISSPTSFYVNTFGTGIIDDNEIQKFIMDNIDLSPYGICKHLLLFNPIYKITS
HHHHEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHC
SYGHFGRIPSLNGSFSWEKEDLALQINRKFHFDKNISHAYI
CCCCCCCCCCCCCCCCCCCCCEEEEEECEEECCCCCCCCCC
>Mature Secondary Structure
MFYYKNCPSSYIITSESVSSGHPDKIADQISDAILDYYISLNPFVHTAIETLVTKNKVFI
CCEECCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCEEEE
AGEICGVNVDNSIIQSIARSVIKEIGYEQDGFHWENVDIEILIHEQSPDIIVGVNSGENQ
EEEEEECCCCHHHHHHHHHHHHHHCCCCCCCEEECCCEEEEEEECCCCCEEEEECCCCCC
GAGDQGIVYGYAINETETLMPASIFYSHLILRNIMDAVKSCEIPKLGPDAKTQITLVYEN
CCCCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEEC
NIPVKVSNVILSIQHPEYITQNQIREIVYPYVSKSLPKGWMCSDSNFLVNTTGRFVIGGP
CCCEEEEEEEEEECCCCHHHHHHHHHHHHHHHHHCCCCCEEECCCCEEEECCCCEEECCC
VGDCGLTGRKIMIDTYGGHVPHGGGAFSGKDPSKVDRSAAYMARYLAKNIVSAKLATKCL
CCCCCCCCCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
VQISYAIGISSPTSFYVNTFGTGIIDDNEIQKFIMDNIDLSPYGICKHLLLFNPIYKITS
HHHHEEEECCCCCEEEEECCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHC
SYGHFGRIPSLNGSFSWEKEDLALQINRKFHFDKNISHAYI
CCCCCCCCCCCCCCCCCCCCCEEEEEECEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA