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The map label for this gene is pyrB

Identifier: 55980751

GI number: 55980751

Start: 750745

End: 751653

Strand: Reverse

Name: pyrB

Synonym: TTHA0782

Alternate gene names: 55980751

Gene position: 751653-750745 (Counterclockwise)

Preceding gene: 55980752

Following gene: 55980750

Centisome position: 40.64

GC content: 69.64

Gene sequence:

>909_bases
ATGAGGCACCTCCTGGACTTCCAGGGCTGGAGCAGGACCGAGGTGGAGAGCCTTCTGGACACCGCCCGGGTGATGCGGGA
GGTCCTGGAGCGGCCCATAAAGAAGGTTCCCGCCCTCCAGGGCTTCACCGTGGCCACGGTCTTCTTTGAGCCCTCCACAA
GGACGAGGATCTCCTTTGAGCTCGCCGCCCGGCGCATGTCGGCGGACGTGGTCTCCTTCGCCGCCCAGACGAGCAGCCTC
CAGAAGGGGGAAAGCTACAAGGACACCCTCCTCACCCTCGAGGCCATGGGGGTGGACGCCTACGTCATCCGGGCCGACAG
CGCCGGCGTACCCCACCAGGCCACCCGCTGGGTCAAGGGCGCCGTCATCAACGGGGGGGACGGGCGCCGGGCCCACCCCA
CCCAGGCCCTCCTGGACGCCTACACCCTCCTCGAGGCCCTGGGCACCCTGGAGGGCAAGAAGGTCGCCATCGTGGGGGAC
ATCCTTCACTCCCGGGTGGCCCGCTCGGGCGCCGAGCTCCTCTCCCTCCTCGGGGCCCAGGTCTTCTGCGCCGGCCCCCC
GAGCCTCCTCCCCCAAAGCCTCCCCGGAGCCCACCTCACCCCCCGTCTGGAGGAGGCCCTGGAGGAGGCGGACGCCGTCA
TGGTCCTCAGGCTCCAGAAGGAGCGGATGGAGGCGGGGCTCGTCCACCTGGAGGACTACGTCGCCCGCTACCAGGTGACG
GAAAAGCGCCTCGCCCTGGCCAAGCCCCAAGCCCCCCTCCTCCACCCCGGCCCCATGAACCGGGACGTGGAGCTGGAAGG
GACCTTGGCGGACTCGGCGAGGAGCCTGGTGAACCGACAGGTGCAAAACGGGGTGGCGGTGCGCATGGCGGTGCTCTACC
ACCTCCTGGTGGGGAGGGAGAAAGCATGA

Upstream 100 bases:

>100_bases
TGGGGAAGAACGTCCCCACCTCCCGGAGCGAGGTGGTGAAGGTCAAGGTGGAGGAGGTGGACGGGGAGGACCGGGTGGAG
CTTTGGGAAAGGGAGGGGGC

Downstream 100 bases:

>100_bases
TCTTGATCCGCAACGTCCGGCTGGTGGACGCCCGGGGCGAGCGGGGCCCCGCGGACGTCCTGATTGGCGAAGGGAGGATC
CTCTCCCTGGAAGGCGGTGA

Product: aspartate carbamoyltransferase catalytic subunit

Products: NA

Alternate protein names: Aspartate transcarbamylase; ATCase

Number of amino acids: Translated: 302; Mature: 302

Protein sequence:

>302_residues
MRHLLDFQGWSRTEVESLLDTARVMREVLERPIKKVPALQGFTVATVFFEPSTRTRISFELAARRMSADVVSFAAQTSSL
QKGESYKDTLLTLEAMGVDAYVIRADSAGVPHQATRWVKGAVINGGDGRRAHPTQALLDAYTLLEALGTLEGKKVAIVGD
ILHSRVARSGAELLSLLGAQVFCAGPPSLLPQSLPGAHLTPRLEEALEEADAVMVLRLQKERMEAGLVHLEDYVARYQVT
EKRLALAKPQAPLLHPGPMNRDVELEGTLADSARSLVNRQVQNGVAVRMAVLYHLLVGREKA

Sequences:

>Translated_302_residues
MRHLLDFQGWSRTEVESLLDTARVMREVLERPIKKVPALQGFTVATVFFEPSTRTRISFELAARRMSADVVSFAAQTSSL
QKGESYKDTLLTLEAMGVDAYVIRADSAGVPHQATRWVKGAVINGGDGRRAHPTQALLDAYTLLEALGTLEGKKVAIVGD
ILHSRVARSGAELLSLLGAQVFCAGPPSLLPQSLPGAHLTPRLEEALEEADAVMVLRLQKERMEAGLVHLEDYVARYQVT
EKRLALAKPQAPLLHPGPMNRDVELEGTLADSARSLVNRQVQNGVAVRMAVLYHLLVGREKA
>Mature_302_residues
MRHLLDFQGWSRTEVESLLDTARVMREVLERPIKKVPALQGFTVATVFFEPSTRTRISFELAARRMSADVVSFAAQTSSL
QKGESYKDTLLTLEAMGVDAYVIRADSAGVPHQATRWVKGAVINGGDGRRAHPTQALLDAYTLLEALGTLEGKKVAIVGD
ILHSRVARSGAELLSLLGAQVFCAGPPSLLPQSLPGAHLTPRLEEALEEADAVMVLRLQKERMEAGLVHLEDYVARYQVT
EKRLALAKPQAPLLHPGPMNRDVELEGTLADSARSLVNRQVQNGVAVRMAVLYHLLVGREKA

Specific function: Pyrimidine biosynthesis; second step. [C]

COG id: COG0540

COG function: function code F; Aspartate carbamoyltransferase, catalytic chain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATCase/OTCase family

Homologues:

Organism=Homo sapiens, GI18105007, Length=304, Percent_Identity=32.8947368421053, Blast_Score=152, Evalue=3e-37,
Organism=Escherichia coli, GI2367364, Length=304, Percent_Identity=31.5789473684211, Blast_Score=124, Evalue=1e-29,
Organism=Caenorhabditis elegans, GI193204318, Length=323, Percent_Identity=31.2693498452012, Blast_Score=121, Evalue=5e-28,
Organism=Saccharomyces cerevisiae, GI6322331, Length=307, Percent_Identity=33.2247557003257, Blast_Score=143, Evalue=3e-35,
Organism=Drosophila melanogaster, GI24642586, Length=315, Percent_Identity=31.4285714285714, Blast_Score=138, Evalue=5e-33,

Paralogues:

None

Copy number: 2080 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 14328 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): PYRB_THET2 (Q72KU0)

Other databases:

- EMBL:   AE017221
- RefSeq:   YP_004402.1
- ProteinModelPortal:   Q72KU0
- STRING:   Q72KU0
- GeneID:   2775964
- GenomeReviews:   AE017221_GR
- eggNOG:   COG0540
- HOGENOM:   HBG579429
- OMA:   MNRGLEI
- ProtClustDB:   PRK00856
- BioCyc:   TTHE262724:TT_C0427-MONOMER
- HAMAP:   MF_00001
- InterPro:   IPR006132
- InterPro:   IPR006130
- InterPro:   IPR006131
- InterPro:   IPR002082
- PRINTS:   PR00100
- PRINTS:   PR00101
- TIGRFAMs:   TIGR00670

Pfam domain/function: PF00185 OTCace; PF02729 OTCace_N; SSF53671 Asp/Orn_carbamoyltranf

EC number: =2.1.3.2

Molecular weight: Translated: 33016; Mature: 33016

Theoretical pI: Translated: 8.46; Mature: 8.46

Prosite motif: PS00097 CARBAMOYLTRANSFERASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRHLLDFQGWSRTEVESLLDTARVMREVLERPIKKVPALQGFTVATVFFEPSTRTRISFE
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCEEHHH
LAARRMSADVVSFAAQTSSLQKGESYKDTLLTLEAMGVDAYVIRADSAGVPHQATRWVKG
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHEECCCCEEEEEECCCCCCHHHHHHHHC
AVINGGDGRRAHPTQALLDAYTLLEALGTLEGKKVAIVGDILHSRVARSGAELLSLLGAQ
EEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCC
VFCAGPPSLLPQSLPGAHLTPRLEEALEEADAVMVLRLQKERMEAGLVHLEDYVARYQVT
EEECCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EKRLALAKPQAPLLHPGPMNRDVELEGTLADSARSLVNRQVQNGVAVRMAVLYHLLVGRE
HHHHHHCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCC
KA
CC
>Mature Secondary Structure
MRHLLDFQGWSRTEVESLLDTARVMREVLERPIKKVPALQGFTVATVFFEPSTRTRISFE
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEECCCCCCEEHHH
LAARRMSADVVSFAAQTSSLQKGESYKDTLLTLEAMGVDAYVIRADSAGVPHQATRWVKG
HHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHEECCCCEEEEEECCCCCCHHHHHHHHC
AVINGGDGRRAHPTQALLDAYTLLEALGTLEGKKVAIVGDILHSRVARSGAELLSLLGAQ
EEEECCCCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHHHHHHHHHHCCC
VFCAGPPSLLPQSLPGAHLTPRLEEALEEADAVMVLRLQKERMEAGLVHLEDYVARYQVT
EEECCCHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EKRLALAKPQAPLLHPGPMNRDVELEGTLADSARSLVNRQVQNGVAVRMAVLYHLLVGRE
HHHHHHCCCCCCCCCCCCCCCCEEEECHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHCCC
KA
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA