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The map label for this gene is ddl [H]

Identifier: 54295449

GI number: 54295449

Start: 2892065

End: 2893159

Strand: Reverse

Name: ddl [H]

Synonym: lpl2535

Alternate gene names: 54295449

Gene position: 2893159-2892065 (Counterclockwise)

Preceding gene: 54295450

Following gene: 54295448

Centisome position: 86.47

GC content: 38.54

Gene sequence:

>1095_bases
ATGTCCAAACCCGTTAATTTGGTTTTACTTTATGGTGGAAAATCAGGCGAGCATGAAGTGTCATTGGTTTCTGCTGCATC
GGTTTTAAAACACCTGGATTCCGAGAAATATCATATTATACCCATTGCAATGGATAAAAGCGGTCGATTTCATCGTCATG
ATTATAATGATTTGCTCGCCTGTTCTGATAAATTACCTGTTGTTACTGAAAAATCAACCCCCTTGGAAGGCTTATTAATT
AATGGACGTCTGGCAGTTGATGCTGAAATTGTTTTTCCAGTGGTTCATGGCCCTTTATATGAAGATGGATGTTTACAAGG
TTTACTTGAGTTAGCCGGTGTCGCCTATGTAGGATGTGACGTATTGTCTTCTGCAATCGGAATGGACAAAGACATGGCGA
GGCGACTCGCATGTATTAATGGGCTCAAATCAGCAAGATATAAATTATTATCATGGCATGCCAATGCGTCTGAAAGACAG
CAATTTTGTCATGAAGTCGCCTCGGAGTTTGGTTGGCCTCTGTTCGTTAAGCCATGCTCACTAGGTTCCAGTGTGGGAAT
TCACAAAGCAAATAATATGGATGAGCTAAACGCAGCAGTTGCTGATGCATTACGCTATGATGAAGAAATTCTGGTTGAAG
AATTTATTGTCGGAAGGGAAATTGAACTGGCAGTGTTGGAGAACAGTATTCCCTGTGGGAAACCAAGAGTAAGTATGGTG
GGTGAGATAAAAGTAAACCACCCTGACGGTTATTATTCTTATACTGCGAAATATTTGGAAAGCAGTCAAACGGATTTGAT
TATTCCAGCTCAATTAAATAATAGTCTGGAAGAACAATTAAAACAGGCTGCAGCGAATATTTTTAGTTATCTTAAATGCA
AAGGCATGGCCAGGGTAGATTTTTTTGTAAATGATAAGACAGAAGAAATTTACTTTAATGAAATAAATACATTACCTGGA
TTTACCTCCATAAGTATGTACCCCAAATTGTGGCAGGCAACTGGTATAGCTTATCCTGATTTACTTGATGAATTGATTAA
TCTAGCAATGATTCATCATAACTGTCGTCAACATTTGGTGACAAATTACTTGTGA

Upstream 100 bases:

>100_bases
GCAGCTAATATTGAAGCCTTGATTCATTTGGTGCAAACTAAAGTTCGAGAACAAACATCTATAGAGTTGATACGCGAAGT
ACATATCATTGGAGACGCTA

Downstream 100 bases:

>100_bases
AGGGAACACTAAATTATGGAAGTGAACTCTGTCGGCTTGCGTTATTTAACGATAATGGGTTTGTTAATTCTTAGTGCTTT
GCTGCTAGCGGGTCGCTTAG

Product: D-alanyl-alanine synthetase A

Products: NA

Alternate protein names: D-Ala-D-Ala ligase; D-alanylalanine synthetase [H]

Number of amino acids: Translated: 364; Mature: 363

Protein sequence:

>364_residues
MSKPVNLVLLYGGKSGEHEVSLVSAASVLKHLDSEKYHIIPIAMDKSGRFHRHDYNDLLACSDKLPVVTEKSTPLEGLLI
NGRLAVDAEIVFPVVHGPLYEDGCLQGLLELAGVAYVGCDVLSSAIGMDKDMARRLACINGLKSARYKLLSWHANASERQ
QFCHEVASEFGWPLFVKPCSLGSSVGIHKANNMDELNAAVADALRYDEEILVEEFIVGREIELAVLENSIPCGKPRVSMV
GEIKVNHPDGYYSYTAKYLESSQTDLIIPAQLNNSLEEQLKQAAANIFSYLKCKGMARVDFFVNDKTEEIYFNEINTLPG
FTSISMYPKLWQATGIAYPDLLDELINLAMIHHNCRQHLVTNYL

Sequences:

>Translated_364_residues
MSKPVNLVLLYGGKSGEHEVSLVSAASVLKHLDSEKYHIIPIAMDKSGRFHRHDYNDLLACSDKLPVVTEKSTPLEGLLI
NGRLAVDAEIVFPVVHGPLYEDGCLQGLLELAGVAYVGCDVLSSAIGMDKDMARRLACINGLKSARYKLLSWHANASERQ
QFCHEVASEFGWPLFVKPCSLGSSVGIHKANNMDELNAAVADALRYDEEILVEEFIVGREIELAVLENSIPCGKPRVSMV
GEIKVNHPDGYYSYTAKYLESSQTDLIIPAQLNNSLEEQLKQAAANIFSYLKCKGMARVDFFVNDKTEEIYFNEINTLPG
FTSISMYPKLWQATGIAYPDLLDELINLAMIHHNCRQHLVTNYL
>Mature_363_residues
SKPVNLVLLYGGKSGEHEVSLVSAASVLKHLDSEKYHIIPIAMDKSGRFHRHDYNDLLACSDKLPVVTEKSTPLEGLLIN
GRLAVDAEIVFPVVHGPLYEDGCLQGLLELAGVAYVGCDVLSSAIGMDKDMARRLACINGLKSARYKLLSWHANASERQQ
FCHEVASEFGWPLFVKPCSLGSSVGIHKANNMDELNAAVADALRYDEEILVEEFIVGREIELAVLENSIPCGKPRVSMVG
EIKVNHPDGYYSYTAKYLESSQTDLIIPAQLNNSLEEQLKQAAANIFSYLKCKGMARVDFFVNDKTEEIYFNEINTLPGF
TSISMYPKLWQATGIAYPDLLDELINLAMIHHNCRQHLVTNYL

Specific function: Cell wall formation [H]

COG id: COG1181

COG function: function code M; D-alanine-D-alanine ligase and related ATP-grasp enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Escherichia coli, GI1786579, Length=366, Percent_Identity=38.7978142076503, Blast_Score=253, Evalue=1e-68,
Organism=Escherichia coli, GI1786280, Length=354, Percent_Identity=31.3559322033898, Blast_Score=139, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR000291
- InterPro:   IPR005905
- InterPro:   IPR011095
- InterPro:   IPR011127
- InterPro:   IPR013817
- InterPro:   IPR016185 [H]

Pfam domain/function: PF07478 Dala_Dala_lig_C; PF01820 Dala_Dala_lig_N [H]

EC number: =6.3.2.4 [H]

Molecular weight: Translated: 40438; Mature: 40307

Theoretical pI: Translated: 5.39; Mature: 5.39

Prosite motif: PS50975 ATP_GRASP ; PS00844 DALA_DALA_LIGASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.5 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.9 %Cys+Met (Translated Protein)
2.5 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKPVNLVLLYGGKSGEHEVSLVSAASVLKHLDSEKYHIIPIAMDKSGRFHRHDYNDLLA
CCCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCHHHHC
CSDKLPVVTEKSTPLEGLLINGRLAVDAEIVFPVVHGPLYEDGCLQGLLELAGVAYVGCD
CCCCCCEEECCCCCCCEEEECCEEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
VLSSAIGMDKDMARRLACINGLKSARYKLLSWHANASERQQFCHEVASEFGWPLFVKPCS
HHHHHHCCCHHHHHHHHHHHHHHHHHEEHEEECCCHHHHHHHHHHHHHHCCCEEEEEECC
LGSSVGIHKANNMDELNAAVADALRYDEEILVEEFIVGREIELAVLENSIPCGKPRVSMV
CCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEEEECCCCCCCCCEEEE
GEIKVNHPDGYYSYTAKYLESSQTDLIIPAQLNNSLEEQLKQAAANIFSYLKCKGMARVD
EEEEEECCCCHHHHHHHHHCCCCCCEEEEHHHCCHHHHHHHHHHHHHHHHHHHCCCEEEE
FFVNDKTEEIYFNEINTLPGFTSISMYPKLWQATGIAYPDLLDELINLAMIHHNCRQHLV
EEECCCCCEEEEEHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
TNYL
HHCC
>Mature Secondary Structure 
SKPVNLVLLYGGKSGEHEVSLVSAASVLKHLDSEKYHIIPIAMDKSGRFHRHDYNDLLA
CCCEEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCEEEEEEEECCCCCCCCCCCHHHHC
CSDKLPVVTEKSTPLEGLLINGRLAVDAEIVFPVVHGPLYEDGCLQGLLELAGVAYVGCD
CCCCCCEEECCCCCCCEEEECCEEEEEEEEEEEECCCCCCCHHHHHHHHHHHHHHHHHHH
VLSSAIGMDKDMARRLACINGLKSARYKLLSWHANASERQQFCHEVASEFGWPLFVKPCS
HHHHHHCCCHHHHHHHHHHHHHHHHHEEHEEECCCHHHHHHHHHHHHHHCCCEEEEEECC
LGSSVGIHKANNMDELNAAVADALRYDEEILVEEFIVGREIELAVLENSIPCGKPRVSMV
CCCCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHCCCCEEEEEEECCCCCCCCCEEEE
GEIKVNHPDGYYSYTAKYLESSQTDLIIPAQLNNSLEEQLKQAAANIFSYLKCKGMARVD
EEEEEECCCCHHHHHHHHHCCCCCCEEEEHHHCCHHHHHHHHHHHHHHHHHHHCCCEEEE
FFVNDKTEEIYFNEINTLPGFTSISMYPKLWQATGIAYPDLLDELINLAMIHHNCRQHLV
EEECCCCCEEEEEHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
TNYL
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA