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The map label for this gene is lepA [H]

Identifier: 54294757

GI number: 54294757

Start: 2050635

End: 2052467

Strand: Reverse

Name: lepA [H]

Synonym: lpl1834

Alternate gene names: 54294757

Gene position: 2052467-2050635 (Counterclockwise)

Preceding gene: 54294761

Following gene: 54294756

Centisome position: 61.35

GC content: 40.15

Gene sequence:

>1833_bases
ATGCTATTTGCAAGAAGGTTAGAACAGTTGAAAGATTTAAACCGAATTCGAAATTTTTCAATAATTGCCCACATTGATCA
TGGCAAGTCTACGTTGGCTGATCGATTTATCCAAATTTGTGGCGGGTTGACTGAGCGCGAAATGAGCTCGCAAGTTCTTG
ATTCTATGGATATTGAACGTGAGCGAGGTATTACTATTAAAGCGCAATGTGTATCCTTGAATTACACAGCTACAGATGGA
AAGACCTATTTATTGAATTTTATCGATACGCCAGGGCATGTTGATTTTAGTTACGAGGTATCTCGTTCTCTTGCCGCTTG
TGAAGGTGCTATTCTGGTTGTTGATGCAGCACAGGGAGTAGAAGCACAAACTTTAGCAGTTTGTTATACAGCAATTGATC
AGTCTCTTACCGTATTGCCAGTACTGAATAAAATTGATTTGCCGCAGGCAGAACCTGAGCGGGTTATTTCCGAGATCGAA
GATATCATTGGTCTAGATGCTCAAGATGCGATTAGAGTGAGTGCTAAAAGTGGCTTGGGTGTTAATGATGTTTTAGAGGC
ATTGGTTGCGAATATTCCCCCTCCGAAAGGCGATGTTCATGCCCCATTACAAGCACTTATTATTGACTCTTGGTTTGATA
GCTATTTAGGAGTTGTCTCACTGGTTCGTATTGTTAATGGCTCCATACGAAAAGGTGATAAAATGCGAGTGATGTCCACA
GGAAGGGCTTATGAAGTGGATCAAGTTGGTATTTTTACCCCCAAACGGACTAAACTGGATGCTTTATATGCTGGTGAAGT
GGGTTATGTTGTTGCTGGTATAAAGGAAATCCAGGGCGCTCCAGTTGGTGACACTTTAACTCTGGATAGAAATCCAGCTG
ATAAAGTTCTTCCAGGGTTTCAACGCGTTAAGCCCCAAGTTTATGCTGGTTTATTTCCGGTTAGCTCGGATGATTTTGAG
GCATTTAGAGAGGCTTTGGCTAAATTAAGTTTAAATGATGCTTCTTTATTTTATGAGCCGGAATCTTCAGAGGCTTTGGG
ATTTGGTTTTCGTTGCGGATTTTTAGGCATGCTGCATATGGAAATCATTCAGGAGAGATTGGAAAGAGAATATAACCTTG
ATTTGATTTCTACGGCACCTACTGTTGTTTATCAGATTGTAACTCAGAAGGGGGAAACTTTATTAATTGATAATCCTTCG
CATTTGCCCCCAACGCCGCAAATAAAAGAGATGTATGAGCCAATTGTTAGAGCAAATATTCTAGTGCCACAAGATTATTT
GGGGCCTATCATTACCTTATGTGTTGAAAGACGTGGTGTTCAAGTCAGTATGACCTATAGTGGTCGTCATGTTTCAGTTG
TTTATGATATTCCCATGAGCGAAGTAGTCTCTGATTTTTTTGATAGATTAAAATCAGTTAGTCGAGGTTATGCCTCTTTG
GATTATAACTTCCAAAGATTCCAGATTGCTGATTTGGTCAAGATGGATATATTAATAAATAGCGAACGTGTTGATGCTCT
TGCTGTGATTGTTCATAGAGATTCCGCTCATAGCCGTGGAAAATTAATTGCGGAGAAGATGCAACAGCTTATTCCAAGAC
AGATGTTTGATGTGGCTATTCAGGCTGCTATAGGAAGCCATATTATTGCCAGGCAAACAGTGAAAGCATTGCGCAAAAAC
GTTACAGCAAAGTGTTATGGTGGTGATGTAACGCGTAAGCGGAAGTTGCTGGAAAAACAAAAAGCTGGCAAGAAAAGAAT
GAAACAGGTGGGGCATGTAGAAATACCTCAAGAAGCATTTATGGCTGTGTTTCAAACAGATAAAAAAAAGTAG

Upstream 100 bases:

>100_bases
AAATTGGTAAATGAATAAGCAATTTTCATTCTCTTAAAAATATAAAAACGACTGAATTTGCTTTGCCTACTTTCTGCATG
CGAACATTTAAGATAGACTT

Downstream 100 bases:

>100_bases
AGCCGCTCATTTACTAATAAGGATGTGCTGACCTTTTCGTCAGCATCTGGACATAAGTCAAATCATGATTAAATTGTCTT
TTGTTTAAAGTTGAGGATAT

Product: GTP-binding protein LepA

Products: NA

Alternate protein names: EF-4; Ribosomal back-translocase LepA [H]

Number of amino acids: Translated: 610; Mature: 610

Protein sequence:

>610_residues
MLFARRLEQLKDLNRIRNFSIIAHIDHGKSTLADRFIQICGGLTEREMSSQVLDSMDIERERGITIKAQCVSLNYTATDG
KTYLLNFIDTPGHVDFSYEVSRSLAACEGAILVVDAAQGVEAQTLAVCYTAIDQSLTVLPVLNKIDLPQAEPERVISEIE
DIIGLDAQDAIRVSAKSGLGVNDVLEALVANIPPPKGDVHAPLQALIIDSWFDSYLGVVSLVRIVNGSIRKGDKMRVMST
GRAYEVDQVGIFTPKRTKLDALYAGEVGYVVAGIKEIQGAPVGDTLTLDRNPADKVLPGFQRVKPQVYAGLFPVSSDDFE
AFREALAKLSLNDASLFYEPESSEALGFGFRCGFLGMLHMEIIQERLEREYNLDLISTAPTVVYQIVTQKGETLLIDNPS
HLPPTPQIKEMYEPIVRANILVPQDYLGPIITLCVERRGVQVSMTYSGRHVSVVYDIPMSEVVSDFFDRLKSVSRGYASL
DYNFQRFQIADLVKMDILINSERVDALAVIVHRDSAHSRGKLIAEKMQQLIPRQMFDVAIQAAIGSHIIARQTVKALRKN
VTAKCYGGDVTRKRKLLEKQKAGKKRMKQVGHVEIPQEAFMAVFQTDKKK

Sequences:

>Translated_610_residues
MLFARRLEQLKDLNRIRNFSIIAHIDHGKSTLADRFIQICGGLTEREMSSQVLDSMDIERERGITIKAQCVSLNYTATDG
KTYLLNFIDTPGHVDFSYEVSRSLAACEGAILVVDAAQGVEAQTLAVCYTAIDQSLTVLPVLNKIDLPQAEPERVISEIE
DIIGLDAQDAIRVSAKSGLGVNDVLEALVANIPPPKGDVHAPLQALIIDSWFDSYLGVVSLVRIVNGSIRKGDKMRVMST
GRAYEVDQVGIFTPKRTKLDALYAGEVGYVVAGIKEIQGAPVGDTLTLDRNPADKVLPGFQRVKPQVYAGLFPVSSDDFE
AFREALAKLSLNDASLFYEPESSEALGFGFRCGFLGMLHMEIIQERLEREYNLDLISTAPTVVYQIVTQKGETLLIDNPS
HLPPTPQIKEMYEPIVRANILVPQDYLGPIITLCVERRGVQVSMTYSGRHVSVVYDIPMSEVVSDFFDRLKSVSRGYASL
DYNFQRFQIADLVKMDILINSERVDALAVIVHRDSAHSRGKLIAEKMQQLIPRQMFDVAIQAAIGSHIIARQTVKALRKN
VTAKCYGGDVTRKRKLLEKQKAGKKRMKQVGHVEIPQEAFMAVFQTDKKK
>Mature_610_residues
MLFARRLEQLKDLNRIRNFSIIAHIDHGKSTLADRFIQICGGLTEREMSSQVLDSMDIERERGITIKAQCVSLNYTATDG
KTYLLNFIDTPGHVDFSYEVSRSLAACEGAILVVDAAQGVEAQTLAVCYTAIDQSLTVLPVLNKIDLPQAEPERVISEIE
DIIGLDAQDAIRVSAKSGLGVNDVLEALVANIPPPKGDVHAPLQALIIDSWFDSYLGVVSLVRIVNGSIRKGDKMRVMST
GRAYEVDQVGIFTPKRTKLDALYAGEVGYVVAGIKEIQGAPVGDTLTLDRNPADKVLPGFQRVKPQVYAGLFPVSSDDFE
AFREALAKLSLNDASLFYEPESSEALGFGFRCGFLGMLHMEIIQERLEREYNLDLISTAPTVVYQIVTQKGETLLIDNPS
HLPPTPQIKEMYEPIVRANILVPQDYLGPIITLCVERRGVQVSMTYSGRHVSVVYDIPMSEVVSDFFDRLKSVSRGYASL
DYNFQRFQIADLVKMDILINSERVDALAVIVHRDSAHSRGKLIAEKMQQLIPRQMFDVAIQAAIGSHIIARQTVKALRKN
VTAKCYGGDVTRKRKLLEKQKAGKKRMKQVGHVEIPQEAFMAVFQTDKKK

Specific function: Required for accurate and efficient protein synthesis under certain stress conditions. May act as a fidelity factor of the translation reaction, by catalyzing a one-codon backward translocation of tRNAs on improperly translocated ribosomes. Back- transloc

COG id: COG0481

COG function: function code M; Membrane GTPase LepA

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. LepA subfamily [H]

Homologues:

Organism=Homo sapiens, GI157426893, Length=607, Percent_Identity=49.2586490939045, Blast_Score=626, Evalue=1e-179,
Organism=Homo sapiens, GI94966754, Length=158, Percent_Identity=43.0379746835443, Blast_Score=119, Evalue=6e-27,
Organism=Homo sapiens, GI4503483, Length=143, Percent_Identity=41.2587412587413, Blast_Score=105, Evalue=1e-22,
Organism=Homo sapiens, GI18390331, Length=159, Percent_Identity=37.1069182389937, Blast_Score=103, Evalue=6e-22,
Organism=Homo sapiens, GI25306283, Length=135, Percent_Identity=45.1851851851852, Blast_Score=102, Evalue=9e-22,
Organism=Homo sapiens, GI25306287, Length=135, Percent_Identity=44.4444444444444, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI19923640, Length=135, Percent_Identity=45.1851851851852, Blast_Score=102, Evalue=1e-21,
Organism=Homo sapiens, GI310132016, Length=115, Percent_Identity=44.3478260869565, Blast_Score=99, Evalue=2e-20,
Organism=Homo sapiens, GI310110807, Length=115, Percent_Identity=44.3478260869565, Blast_Score=99, Evalue=2e-20,
Organism=Homo sapiens, GI310123363, Length=115, Percent_Identity=44.3478260869565, Blast_Score=99, Evalue=2e-20,
Organism=Homo sapiens, GI217272892, Length=134, Percent_Identity=39.5522388059701, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI217272894, Length=133, Percent_Identity=36.8421052631579, Blast_Score=91, Evalue=3e-18,
Organism=Homo sapiens, GI53729339, Length=239, Percent_Identity=30.5439330543933, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI53729337, Length=239, Percent_Identity=30.5439330543933, Blast_Score=86, Evalue=1e-16,
Organism=Homo sapiens, GI94966752, Length=94, Percent_Identity=43.6170212765958, Blast_Score=73, Evalue=8e-13,
Organism=Escherichia coli, GI1788922, Length=596, Percent_Identity=72.1476510067114, Blast_Score=888, Evalue=0.0,
Organism=Escherichia coli, GI48994988, Length=509, Percent_Identity=27.7013752455796, Blast_Score=164, Evalue=2e-41,
Organism=Escherichia coli, GI1789738, Length=159, Percent_Identity=33.9622641509434, Blast_Score=91, Evalue=3e-19,
Organism=Escherichia coli, GI1790835, Length=159, Percent_Identity=31.4465408805031, Blast_Score=87, Evalue=2e-18,
Organism=Escherichia coli, GI1789559, Length=254, Percent_Identity=28.3464566929134, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17557151, Length=612, Percent_Identity=40.359477124183, Blast_Score=488, Evalue=1e-138,
Organism=Caenorhabditis elegans, GI17556745, Length=465, Percent_Identity=25.3763440860215, Blast_Score=121, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI17533571, Length=164, Percent_Identity=32.9268292682927, Blast_Score=99, Evalue=5e-21,
Organism=Caenorhabditis elegans, GI17506493, Length=155, Percent_Identity=37.4193548387097, Blast_Score=96, Evalue=4e-20,
Organism=Caenorhabditis elegans, GI71988819, Length=150, Percent_Identity=36, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI71988811, Length=150, Percent_Identity=36, Blast_Score=90, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI17552882, Length=171, Percent_Identity=33.3333333333333, Blast_Score=89, Evalue=5e-18,
Organism=Caenorhabditis elegans, GI71994658, Length=223, Percent_Identity=29.5964125560538, Blast_Score=73, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6323320, Length=609, Percent_Identity=45.8128078817734, Blast_Score=537, Evalue=1e-153,
Organism=Saccharomyces cerevisiae, GI6324707, Length=162, Percent_Identity=38.8888888888889, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6320593, Length=162, Percent_Identity=38.8888888888889, Blast_Score=112, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6323098, Length=180, Percent_Identity=36.1111111111111, Blast_Score=108, Evalue=3e-24,
Organism=Saccharomyces cerevisiae, GI6322359, Length=137, Percent_Identity=36.4963503649635, Blast_Score=94, Evalue=4e-20,
Organism=Saccharomyces cerevisiae, GI6324166, Length=197, Percent_Identity=34.5177664974619, Blast_Score=89, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6324761, Length=286, Percent_Identity=27.2727272727273, Blast_Score=70, Evalue=8e-13,
Organism=Saccharomyces cerevisiae, GI6322675, Length=141, Percent_Identity=30.4964539007092, Blast_Score=67, Evalue=1e-11,
Organism=Drosophila melanogaster, GI78706572, Length=602, Percent_Identity=44.0199335548173, Blast_Score=531, Evalue=1e-151,
Organism=Drosophila melanogaster, GI24582462, Length=168, Percent_Identity=36.9047619047619, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI28574573, Length=152, Percent_Identity=42.7631578947368, Blast_Score=103, Evalue=5e-22,
Organism=Drosophila melanogaster, GI221458488, Length=151, Percent_Identity=39.7350993377483, Blast_Score=100, Evalue=3e-21,
Organism=Drosophila melanogaster, GI24585709, Length=147, Percent_Identity=37.4149659863946, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24585711, Length=147, Percent_Identity=37.4149659863946, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI24585713, Length=147, Percent_Identity=37.4149659863946, Blast_Score=97, Evalue=3e-20,
Organism=Drosophila melanogaster, GI21357743, Length=135, Percent_Identity=36.2962962962963, Blast_Score=90, Evalue=4e-18,
Organism=Drosophila melanogaster, GI28572034, Length=233, Percent_Identity=28.3261802575107, Blast_Score=74, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009022
- InterPro:   IPR006297
- InterPro:   IPR013842
- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR000640
- InterPro:   IPR004161
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00679 EFG_C; PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF06421 LepA_C [H]

EC number: NA

Molecular weight: Translated: 67745; Mature: 67745

Theoretical pI: Translated: 6.74; Mature: 6.74

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLFARRLEQLKDLNRIRNFSIIAHIDHGKSTLADRFIQICGGLTEREMSSQVLDSMDIER
CCHHHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHH
ERGITIKAQCVSLNYTATDGKTYLLNFIDTPGHVDFSYEVSRSLAACEGAILVVDAAQGV
HCCCEEEEEEEEEEEEECCCCEEEEEEECCCCCEEEEHHHHHHHHHHCCCEEEEECCCCC
EAQTLAVCYTAIDQSLTVLPVLNKIDLPQAEPERVISEIEDIIGLDAQDAIRVSAKSGLG
CHHHHHHHHHHHCCCCEEEEHHCCCCCCCCCHHHHHHHHHHHHCCCCHHHEEEECCCCCC
VNDVLEALVANIPPPKGDVHAPLQALIIDSWFDSYLGVVSLVRIVNGSIRKGDKMRVMST
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEC
GRAYEVDQVGIFTPKRTKLDALYAGEVGYVVAGIKEIQGAPVGDTLTLDRNPADKVLPGF
CCEEEECCEEEECCCCCCCCEEECCCHHHHHHHHHHHCCCCCCCEEEECCCCCHHHCCCH
QRVKPQVYAGLFPVSSDDFEAFREALAKLSLNDASLFYEPESSEALGFGFRCGFLGMLHM
HHHCCHHHHEECCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHH
EIIQERLEREYNLDLISTAPTVVYQIVTQKGETLLIDNPSHLPPTPQIKEMYEPIVRANI
HHHHHHHHHHCCCEEECCCHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHCEE
LVPQDYLGPIITLCVERRGVQVSMTYSGRHVSVVYDIPMSEVVSDFFDRLKSVSRGYASL
ECCHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEEECCHHHHHHHHHHHHHHHHCCCEEC
DYNFQRFQIADLVKMDILINSERVDALAVIVHRDSAHSRGKLIAEKMQQLIPRQMFDVAI
CCCCHHHHHHHHHHHHEEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
QAAIGSHIIARQTVKALRKNVTAKCYGGDVTRKRKLLEKQKAGKKRMKQVGHVEIPQEAF
HHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
MAVFQTDKKK
HHHHHHCCCC
>Mature Secondary Structure
MLFARRLEQLKDLNRIRNFSIIAHIDHGKSTLADRFIQICGGLTEREMSSQVLDSMDIER
CCHHHHHHHHHHHHHHCCEEEEEEECCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCHH
ERGITIKAQCVSLNYTATDGKTYLLNFIDTPGHVDFSYEVSRSLAACEGAILVVDAAQGV
HCCCEEEEEEEEEEEEECCCCEEEEEEECCCCCEEEEHHHHHHHHHHCCCEEEEECCCCC
EAQTLAVCYTAIDQSLTVLPVLNKIDLPQAEPERVISEIEDIIGLDAQDAIRVSAKSGLG
CHHHHHHHHHHHCCCCEEEEHHCCCCCCCCCHHHHHHHHHHHHCCCCHHHEEEECCCCCC
VNDVLEALVANIPPPKGDVHAPLQALIIDSWFDSYLGVVSLVRIVNGSIRKGDKMRVMST
HHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEC
GRAYEVDQVGIFTPKRTKLDALYAGEVGYVVAGIKEIQGAPVGDTLTLDRNPADKVLPGF
CCEEEECCEEEECCCCCCCCEEECCCHHHHHHHHHHHCCCCCCCEEEECCCCCHHHCCCH
QRVKPQVYAGLFPVSSDDFEAFREALAKLSLNDASLFYEPESSEALGFGFRCGFLGMLHM
HHHCCHHHHEECCCCCCHHHHHHHHHHHCCCCCCEEEECCCCCCCCCCCHHHHHHHHHHH
EIIQERLEREYNLDLISTAPTVVYQIVTQKGETLLIDNPSHLPPTPQIKEMYEPIVRANI
HHHHHHHHHHCCCEEECCCHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHCEE
LVPQDYLGPIITLCVERRGVQVSMTYSGRHVSVVYDIPMSEVVSDFFDRLKSVSRGYASL
ECCHHHHHHHHHHHHHCCCCEEEEEECCCEEEEEEECCHHHHHHHHHHHHHHHHCCCEEC
DYNFQRFQIADLVKMDILINSERVDALAVIVHRDSAHSRGKLIAEKMQQLIPRQMFDVAI
CCCCHHHHHHHHHHHHEEECCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHH
QAAIGSHIIARQTVKALRKNVTAKCYGGDVTRKRKLLEKQKAGKKRMKQVGHVEIPQEAF
HHHHHHHHHHHHHHHHHHHCCCEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHH
MAVFQTDKKK
HHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA