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The map label for this gene is alaS [H]

Identifier: 54294686

GI number: 54294686

Start: 1965660

End: 1968242

Strand: Reverse

Name: alaS [H]

Synonym: lpl1763

Alternate gene names: 54294686

Gene position: 1968242-1965660 (Counterclockwise)

Preceding gene: 54294687

Following gene: 54294685

Centisome position: 58.83

GC content: 39.53

Gene sequence:

>2583_bases
ATGAAAAGTTCTGAAATTAGACAAGCATTCCTTAATTATTTTGTTCAACGCGGTCATCAATTAGTAGCTTCTAGTTCATT
GATCCCTTCAAATGACCCCACTTTGCTATTTACCAATGCAGGTATGGTGCAATTCAAGGATCTTTTTTTAGGTCTTGAAA
CCCGTCCTTACCAGCGTGCTGTTACAGCGCAGCGCTGTGTGCGTGCGGGTGGTAAACATAACGATCTGGAAAATGTTGGC
TATACGGCGAGACACCATACATTTTTTGAAATGTTGGGTAATTTTAGTTTTGGCGATTATTTTAAACGCGAAGCCATTCA
ATATGCTTGGGAATTTCTAACAGAAGTTTTACACATACCGGCAGAACGGCTATGGGTTACTGTCTATAAAGAGGATTTGG
AAGCAGAAGGCATATGGCTAAAAGAAATGAAGGTATCTCCCGAGCGATTCTCTCGATGTGGGGAAAAAGATAATTTCTGG
TCAATGGGGGATACGGGTCCATGTGGTCCCTGTACGGAAATTTTTTATGATCATGGTCCTGAAGTGGCAGGTGGACCTCC
AGGCAGTCCAGATGAAGACGGGGATCGTTATATAGAAATATGGAATCTGGTATTCATGCAATTTAACAGGGACAGAGAGG
GATGCTTGCACCCTTTGCCAAAGCCCTCCGTTGATACTGGCATGGGATTGGAGAGATTAGCTGCGGTTATTCAAGGTGTT
CATAGTAATTATGAAATTGATAGTTTTCAGTATTTGATCAAAGCAATAGCACAATTGGGCCAGGATATTGATTTAAATCA
TACTTCCTTAAAAGTGATCGCAGATCATATTCGTTCCTGCTCTTTTTTGATAGTTGATGGAGTTTTACCTAGTAATGAAG
GCAGAGGATATGTATTAAGAAGGATTATCCGCAGAGCAGTCAGGCATGGGAATAAATTAGGTTTACCCAGCCCATTTTTT
TTCAAACTTGTTCAGCCTTTGATTGATGTCATGGGAGATGCTTATCCAGAGCTAATCAATTCGAAAGCGCATATCGAAAG
GATCTTGCAACAAGAAGAAAATCAGTTTACTCGTACTTTGGAACAAGGATTGCGTTTATTGCAAGATCATATTAAAAATT
TGAAAGGCCAGGAACTATCAGGAGAAGTGGCATTCAAGTTGTATGATACTTATGGTTTCCCTATTGATTTGACAGCAGAC
ATCATTCGTGAACAAGGTTTGCATATTGATATGGAAGCCTTTAATCAGCTGATGCAACAGCAAAGAGAACAGTCTCAAGC
CGCCAGTCAGTTTACTACAGATTATCATGCCGTGTCTCAATTGGATCATCAGTCTGAATTTCATGGGTATGAAAAAGAAT
CCATGGAAGCAAAGATTATTGGATTACTTCAAGAAGGTAATGAAGTGAAATCTCTTAATAAGGGAGCAAAAGGGGCTGTA
ATTCTTGATCATACCCCATTTTATGCTGAAAGTGGTGGTCAGGTAGGAGATAAAGGTTTACTCATAGGAAAAGAATTTTC
TTTTCAAGTGGATGATACACAAAAAGTAGGGCAAGCCGTCGTACATTATGGAGAGGTAATCAAAGGCGAGCTCACTTTAG
ATTTATCAATCCATGCGCAGGTAGATCACATAAGACGTGATGCAATTCGATTGAATCATACTGCAACTCATTTGTTACAT
GCTGCTCTAAAACAAATTGTAGGACAACATGTTCAACAACGAGGATCTTTGGTGGATGCTGAACGGGCACGTTTTGACTT
TTCTCATTTTGAGCCATTGACCCCTCAACAAATCCAGCAAATTGAAGAGGTAGTTAATGCACAGATTCGAGCTAATAATG
AAGTAATAACTCAAGTAATGGACATTGAATCAGCCAAGCAAAGTGGTGCTGTTGCTTTATTCGGGGAAAAATACTCCGAT
GCAGTACGGGTATTATCCATGGGTGACTTTTCCAAGGAGCTTTGTGGAGGAACTCATGCACGACGTACTGGTGATATAGG
CCTTTTCAAAATCGTAGCAGAATATGGTATTGCCAGCGGTATCAGGCGGGTTGAGATGGTTACGGGTCGTTATGCATTAG
CCTGGGTCAATGAGCAGCTGGGCTTTATGAATAATCTGGCAGCTACCCTTAAAACAACACCTAATAGTCTACAGGAAAAA
GTTTCTCAATTATTGCTTGATAATAAAAATCAAGAAAAAATGATTGCAAAACTTTTAAGTGAAAAAGCACAAAAATCTGG
AGCAGATATTCTTGGTGAGATCGAGGAGATCAAGGGTATTAATTTATTGATTAAGCAGTTAGAGGGGATGGATAGCCAAA
CCATGCGCCATACCATGGATCAATTAAAATCAAGAATTGATTCAGCGGTGATCATATTATTTACTATTGAACAGAATAAA
ATGAATGTCATTGCTGGGGTGAGTAAGAATATTATTGGCAAAACGCCAAATGCTGCTCAATTAGTAAGACATCTTTGTGG
TAAAGGAGGTGGTCGAGATGATATGGCTCAAGGTGGTGGGGACGTTCCTGAAGATTTGAACAGTAAGATCAAAGAAATAA
AAGAAATGATTGAAAAAAATTAA

Upstream 100 bases:

>100_bases
ATCGCTTTTTGCTGTATCGAGGGTTTGATAGGGATGTTATTTCAAAGGTATTTAAAGAGGTTAAATCTTCTTACTTAATT
TAACTGGTAAATATTAAAAA

Downstream 100 bases:

>100_bases
TATTTCCAGATTTTTCTTTTCGACTGCTGAAAAGCAAGTTGTTTTTGGATTTGCTATAATATAGACACTGGTTTACTAAT
GAGCACTATGATGAATGACT

Product: alanyl-tRNA synthetase

Products: NA

Alternate protein names: Alanine--tRNA ligase; AlaRS [H]

Number of amino acids: Translated: 860; Mature: 860

Protein sequence:

>860_residues
MKSSEIRQAFLNYFVQRGHQLVASSSLIPSNDPTLLFTNAGMVQFKDLFLGLETRPYQRAVTAQRCVRAGGKHNDLENVG
YTARHHTFFEMLGNFSFGDYFKREAIQYAWEFLTEVLHIPAERLWVTVYKEDLEAEGIWLKEMKVSPERFSRCGEKDNFW
SMGDTGPCGPCTEIFYDHGPEVAGGPPGSPDEDGDRYIEIWNLVFMQFNRDREGCLHPLPKPSVDTGMGLERLAAVIQGV
HSNYEIDSFQYLIKAIAQLGQDIDLNHTSLKVIADHIRSCSFLIVDGVLPSNEGRGYVLRRIIRRAVRHGNKLGLPSPFF
FKLVQPLIDVMGDAYPELINSKAHIERILQQEENQFTRTLEQGLRLLQDHIKNLKGQELSGEVAFKLYDTYGFPIDLTAD
IIREQGLHIDMEAFNQLMQQQREQSQAASQFTTDYHAVSQLDHQSEFHGYEKESMEAKIIGLLQEGNEVKSLNKGAKGAV
ILDHTPFYAESGGQVGDKGLLIGKEFSFQVDDTQKVGQAVVHYGEVIKGELTLDLSIHAQVDHIRRDAIRLNHTATHLLH
AALKQIVGQHVQQRGSLVDAERARFDFSHFEPLTPQQIQQIEEVVNAQIRANNEVITQVMDIESAKQSGAVALFGEKYSD
AVRVLSMGDFSKELCGGTHARRTGDIGLFKIVAEYGIASGIRRVEMVTGRYALAWVNEQLGFMNNLAATLKTTPNSLQEK
VSQLLLDNKNQEKMIAKLLSEKAQKSGADILGEIEEIKGINLLIKQLEGMDSQTMRHTMDQLKSRIDSAVIILFTIEQNK
MNVIAGVSKNIIGKTPNAAQLVRHLCGKGGGRDDMAQGGGDVPEDLNSKIKEIKEMIEKN

Sequences:

>Translated_860_residues
MKSSEIRQAFLNYFVQRGHQLVASSSLIPSNDPTLLFTNAGMVQFKDLFLGLETRPYQRAVTAQRCVRAGGKHNDLENVG
YTARHHTFFEMLGNFSFGDYFKREAIQYAWEFLTEVLHIPAERLWVTVYKEDLEAEGIWLKEMKVSPERFSRCGEKDNFW
SMGDTGPCGPCTEIFYDHGPEVAGGPPGSPDEDGDRYIEIWNLVFMQFNRDREGCLHPLPKPSVDTGMGLERLAAVIQGV
HSNYEIDSFQYLIKAIAQLGQDIDLNHTSLKVIADHIRSCSFLIVDGVLPSNEGRGYVLRRIIRRAVRHGNKLGLPSPFF
FKLVQPLIDVMGDAYPELINSKAHIERILQQEENQFTRTLEQGLRLLQDHIKNLKGQELSGEVAFKLYDTYGFPIDLTAD
IIREQGLHIDMEAFNQLMQQQREQSQAASQFTTDYHAVSQLDHQSEFHGYEKESMEAKIIGLLQEGNEVKSLNKGAKGAV
ILDHTPFYAESGGQVGDKGLLIGKEFSFQVDDTQKVGQAVVHYGEVIKGELTLDLSIHAQVDHIRRDAIRLNHTATHLLH
AALKQIVGQHVQQRGSLVDAERARFDFSHFEPLTPQQIQQIEEVVNAQIRANNEVITQVMDIESAKQSGAVALFGEKYSD
AVRVLSMGDFSKELCGGTHARRTGDIGLFKIVAEYGIASGIRRVEMVTGRYALAWVNEQLGFMNNLAATLKTTPNSLQEK
VSQLLLDNKNQEKMIAKLLSEKAQKSGADILGEIEEIKGINLLIKQLEGMDSQTMRHTMDQLKSRIDSAVIILFTIEQNK
MNVIAGVSKNIIGKTPNAAQLVRHLCGKGGGRDDMAQGGGDVPEDLNSKIKEIKEMIEKN
>Mature_860_residues
MKSSEIRQAFLNYFVQRGHQLVASSSLIPSNDPTLLFTNAGMVQFKDLFLGLETRPYQRAVTAQRCVRAGGKHNDLENVG
YTARHHTFFEMLGNFSFGDYFKREAIQYAWEFLTEVLHIPAERLWVTVYKEDLEAEGIWLKEMKVSPERFSRCGEKDNFW
SMGDTGPCGPCTEIFYDHGPEVAGGPPGSPDEDGDRYIEIWNLVFMQFNRDREGCLHPLPKPSVDTGMGLERLAAVIQGV
HSNYEIDSFQYLIKAIAQLGQDIDLNHTSLKVIADHIRSCSFLIVDGVLPSNEGRGYVLRRIIRRAVRHGNKLGLPSPFF
FKLVQPLIDVMGDAYPELINSKAHIERILQQEENQFTRTLEQGLRLLQDHIKNLKGQELSGEVAFKLYDTYGFPIDLTAD
IIREQGLHIDMEAFNQLMQQQREQSQAASQFTTDYHAVSQLDHQSEFHGYEKESMEAKIIGLLQEGNEVKSLNKGAKGAV
ILDHTPFYAESGGQVGDKGLLIGKEFSFQVDDTQKVGQAVVHYGEVIKGELTLDLSIHAQVDHIRRDAIRLNHTATHLLH
AALKQIVGQHVQQRGSLVDAERARFDFSHFEPLTPQQIQQIEEVVNAQIRANNEVITQVMDIESAKQSGAVALFGEKYSD
AVRVLSMGDFSKELCGGTHARRTGDIGLFKIVAEYGIASGIRRVEMVTGRYALAWVNEQLGFMNNLAATLKTTPNSLQEK
VSQLLLDNKNQEKMIAKLLSEKAQKSGADILGEIEEIKGINLLIKQLEGMDSQTMRHTMDQLKSRIDSAVIILFTIEQNK
MNVIAGVSKNIIGKTPNAAQLVRHLCGKGGGRDDMAQGGGDVPEDLNSKIKEIKEMIEKN

Specific function: Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction:alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editin

COG id: COG0013

COG function: function code J; Alanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI109148542, Length=953, Percent_Identity=37.3557187827912, Blast_Score=541, Evalue=1e-154,
Organism=Homo sapiens, GI38569417, Length=889, Percent_Identity=35.8830146231721, Blast_Score=476, Evalue=1e-134,
Organism=Escherichia coli, GI1789048, Length=851, Percent_Identity=56.8742655699177, Blast_Score=1017, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17506981, Length=969, Percent_Identity=36.2229102167183, Blast_Score=541, Evalue=1e-154,
Organism=Caenorhabditis elegans, GI17536681, Length=778, Percent_Identity=33.8046272493573, Blast_Score=407, Evalue=1e-114,
Organism=Saccharomyces cerevisiae, GI6324911, Length=779, Percent_Identity=41.0783055198973, Blast_Score=563, Evalue=1e-161,
Organism=Drosophila melanogaster, GI24582809, Length=965, Percent_Identity=36.1658031088083, Blast_Score=560, Evalue=1e-159,
Organism=Drosophila melanogaster, GI45552267, Length=965, Percent_Identity=36.1658031088083, Blast_Score=560, Evalue=1e-159,
Organism=Drosophila melanogaster, GI24658214, Length=777, Percent_Identity=31.5315315315315, Blast_Score=337, Evalue=1e-92,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002318
- InterPro:   IPR018162
- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR023033
- InterPro:   IPR003156
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF02272 DHHA1; PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: =6.1.1.7 [H]

Molecular weight: Translated: 96391; Mature: 96391

Theoretical pI: Translated: 6.14; Mature: 6.14

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSSEIRQAFLNYFVQRGHQLVASSSLIPSNDPTLLFTNAGMVQFKDLFLGLETRPYQRA
CCHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHCCCCCCHHHH
VTAQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKREAIQYAWEFLTEVLHIP
HHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCC
AERLWVTVYKEDLEAEGIWLKEMKVSPERFSRCGEKDNFWSMGDTGPCGPCTEIFYDHGP
HHHHEEEEEHHCCCCCCEEHHHHCCCHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCC
EVAGGPPGSPDEDGDRYIEIWNLVFMQFNRDREGCLHPLPKPSVDTGMGLERLAAVIQGV
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
HSNYEIDSFQYLIKAIAQLGQDIDLNHTSLKVIADHIRSCSFLIVDGVLPSNEGRGYVLR
HCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHH
RIIRRAVRHGNKLGLPSPFFFKLVQPLIDVMGDAYPELINSKAHIERILQQEENQFTRTL
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHHH
EQGLRLLQDHIKNLKGQELSGEVAFKLYDTYGFPIDLTADIIREQGLHIDMEAFNQLMQQ
HHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHCCCEEEHHHHHHHHHH
QREQSQAASQFTTDYHAVSQLDHQSEFHGYEKESMEAKIIGLLQEGNEVKSLNKGAKGAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHHCCCHHHHHCCCCCCCE
ILDHTPFYAESGGQVGDKGLLIGKEFSFQVDDTQKVGQAVVHYGEVIKGELTLDLSIHAQ
EEECCCCCCCCCCCCCCCCEEEECCCEEEECCHHHHHHHHHHHHHHHCCEEEEEEEEHHH
VDHIRRDAIRLNHTATHLLHAALKQIVGQHVQQRGSLVDAERARFDFSHFEPLTPQQIQQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCHHHCCCCCHHHHHH
IEEVVNAQIRANNEVITQVMDIESAKQSGAVALFGEKYSDAVRVLSMGDFSKELCGGTHA
HHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHCCCHHHHHHCCCCC
RRTGDIGLFKIVAEYGIASGIRRVEMVTGRYALAWVNEQLGFMNNLAATLKTTPNSLQEK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCHHHHHHH
VSQLLLDNKNQEKMIAKLLSEKAQKSGADILGEIEEIKGINLLIKQLEGMDSQTMRHTMD
HHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
QLKSRIDSAVIILFTIEQNKMNVIAGVSKNIIGKTPNAAQLVRHLCGKGGGRDDMAQGGG
HHHHHCCCEEEEEEEECCCCCEEECCCHHHHCCCCCCHHHHHHHHHCCCCCCCHHHCCCC
DVPEDLNSKIKEIKEMIEKN
CCCHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKSSEIRQAFLNYFVQRGHQLVASSSLIPSNDPTLLFTNAGMVQFKDLFLGLETRPYQRA
CCHHHHHHHHHHHHHHHCHHHHHCCCCCCCCCCEEEEECCCHHHHHHHHHCCCCCCHHHH
VTAQRCVRAGGKHNDLENVGYTARHHTFFEMLGNFSFGDYFKREAIQYAWEFLTEVLHIP
HHHHHHHHCCCCCCCHHHCCCCHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCC
AERLWVTVYKEDLEAEGIWLKEMKVSPERFSRCGEKDNFWSMGDTGPCGPCTEIFYDHGP
HHHHEEEEEHHCCCCCCEEHHHHCCCHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCCC
EVAGGPPGSPDEDGDRYIEIWNLVFMQFNRDREGCLHPLPKPSVDTGMGLERLAAVIQGV
CCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHH
HSNYEIDSFQYLIKAIAQLGQDIDLNHTSLKVIADHIRSCSFLIVDGVLPSNEGRGYVLR
HCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHHH
RIIRRAVRHGNKLGLPSPFFFKLVQPLIDVMGDAYPELINSKAHIERILQQEENQFTRTL
HHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHHH
EQGLRLLQDHIKNLKGQELSGEVAFKLYDTYGFPIDLTADIIREQGLHIDMEAFNQLMQQ
HHHHHHHHHHHHCCCCCCCCCCEEEEEEECCCCCCCHHHHHHHHCCCEEEHHHHHHHHHH
QREQSQAASQFTTDYHAVSQLDHQSEFHGYEKESMEAKIIGLLQEGNEVKSLNKGAKGAV
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHCCHHHHHHHHHCCCHHHHHCCCCCCCE
ILDHTPFYAESGGQVGDKGLLIGKEFSFQVDDTQKVGQAVVHYGEVIKGELTLDLSIHAQ
EEECCCCCCCCCCCCCCCCEEEECCCEEEECCHHHHHHHHHHHHHHHCCEEEEEEEEHHH
VDHIRRDAIRLNHTATHLLHAALKQIVGQHVQQRGSLVDAERARFDFSHFEPLTPQQIQQ
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHCCHHHCCCCCHHHHHH
IEEVVNAQIRANNEVITQVMDIESAKQSGAVALFGEKYSDAVRVLSMGDFSKELCGGTHA
HHHHHHHHHHCCHHHHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHCCCHHHHHHCCCCC
RRTGDIGLFKIVAEYGIASGIRRVEMVTGRYALAWVNEQLGFMNNLAATLKTTPNSLQEK
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEHHHHHHHHHHHHHHHHCCHHHHHHH
VSQLLLDNKNQEKMIAKLLSEKAQKSGADILGEIEEIKGINLLIKQLEGMDSQTMRHTMD
HHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
QLKSRIDSAVIILFTIEQNKMNVIAGVSKNIIGKTPNAAQLVRHLCGKGGGRDDMAQGGG
HHHHHCCCEEEEEEEECCCCCEEECCCHHHHCCCCCCHHHHHHHHHCCCCCCCHHHCCCC
DVPEDLNSKIKEIKEMIEKN
CCCHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA