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The map label for this gene is fliA [H]

Identifier: 54294669

GI number: 54294669

Start: 1950332

End: 1951048

Strand: Reverse

Name: fliA [H]

Synonym: lpl1746

Alternate gene names: 54294669

Gene position: 1951048-1950332 (Counterclockwise)

Preceding gene: 54294670

Following gene: 54294668

Centisome position: 58.32

GC content: 41.98

Gene sequence:

>717_bases
GTGGATGCTTTGGCTGCATACAGCAAAGTAAATCAACAAACCCAGGAAGCTCTGGTAAAAACACATGCCACATTGGTAAA
ACGCATTGCACACCATCTGCTCGGGCGATTACCGCAGAGTGTACAACTGGATGATTTAATACAATCAGGGATGCTCGGTC
TGCTTGAAGCAGCAAGACATTATGATTCATCCAAGGGGGCATCCTTTGAAACTTATGCAGGAATACGTATCAGGGGACAT
ATGCTTGATGAAGTAAGACGTAACGATTGGGTGCCACGTTCTGTTTATCGCAATTCCAGAATGATTTCTGATGCTGTGCG
CATATTGGAGCATAAGTTTGGGCGTGAGGCTAAGGATAACGAAATTGCTGAAGAGCTAGGCGTGTCATTAAATGATTATC
ATGATATGTTGCAAGACTCCATTAGCAGTCATTTATATGGTTTTGATGACTTGGGAGTAACCGATGATGTCTTATTTGAT
GATGAAGGGAATGGCTCAACTGAACCGCACAGGAATGTTATGCGAAGTGATATGATGAATCGATTAACCCAAGTGATTGA
TAGTCTCCCTCGTAAAGAGAGACTGGTGCTTTCTTTGTATTATGAGCAGGATTTGAATTTGAAGGAAATCGGGGAAATAC
TGGAGGTTAGTGAGTCACGAATTTCACAGATTTTAAGTCAAGCAACGCACCGTATTAGATCAAGATTACCGGAATAA

Upstream 100 bases:

>100_bases
TTAAATTATTCGCTATACTTAAAATTAGCTGTACTTTGTTTGTTTTAGATTTACCAAATTACTAATAACTACAATCTGCT
GTTATGGTAAGGAGAGTATC

Downstream 100 bases:

>100_bases
AAAATAAGTATGGATACTTTGACTTTAATAGGATTGTTGACAGGTTTTCTCGCCATTATCGTAGGCCAAATGTTTGAGGG
GGGAGATTTAAATTCCTTAC

Product: flagellar biosynthesis sigma factor

Products: NA

Alternate protein names: Sigma-28; Sigma-F factor [H]

Number of amino acids: Translated: 238; Mature: 238

Protein sequence:

>238_residues
MDALAAYSKVNQQTQEALVKTHATLVKRIAHHLLGRLPQSVQLDDLIQSGMLGLLEAARHYDSSKGASFETYAGIRIRGH
MLDEVRRNDWVPRSVYRNSRMISDAVRILEHKFGREAKDNEIAEELGVSLNDYHDMLQDSISSHLYGFDDLGVTDDVLFD
DEGNGSTEPHRNVMRSDMMNRLTQVIDSLPRKERLVLSLYYEQDLNLKEIGEILEVSESRISQILSQATHRIRSRLPE

Sequences:

>Translated_238_residues
MDALAAYSKVNQQTQEALVKTHATLVKRIAHHLLGRLPQSVQLDDLIQSGMLGLLEAARHYDSSKGASFETYAGIRIRGH
MLDEVRRNDWVPRSVYRNSRMISDAVRILEHKFGREAKDNEIAEELGVSLNDYHDMLQDSISSHLYGFDDLGVTDDVLFD
DEGNGSTEPHRNVMRSDMMNRLTQVIDSLPRKERLVLSLYYEQDLNLKEIGEILEVSESRISQILSQATHRIRSRLPE
>Mature_238_residues
MDALAAYSKVNQQTQEALVKTHATLVKRIAHHLLGRLPQSVQLDDLIQSGMLGLLEAARHYDSSKGASFETYAGIRIRGH
MLDEVRRNDWVPRSVYRNSRMISDAVRILEHKFGREAKDNEIAEELGVSLNDYHDMLQDSISSHLYGFDDLGVTDDVLFD
DEGNGSTEPHRNVMRSDMMNRLTQVIDSLPRKERLVLSLYYEQDLNLKEIGEILEVSESRISQILSQATHRIRSRLPE

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This alternative sigma factor is specific for the flagellin gene (fliC) expression [H]

COG id: COG1191

COG function: function code K; DNA-directed RNA polymerase specialized sigma subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family [H]

Homologues:

Organism=Escherichia coli, GI1788231, Length=224, Percent_Identity=45.5357142857143, Blast_Score=199, Evalue=1e-52,
Organism=Escherichia coli, GI1789098, Length=229, Percent_Identity=27.9475982532751, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR007627
- InterPro:   IPR007624
- InterPro:   IPR007630
- InterPro:   IPR012845
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR011991 [H]

Pfam domain/function: PF04542 Sigma70_r2; PF04539 Sigma70_r3; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 27187; Mature: 27187

Theoretical pI: Translated: 5.79; Mature: 5.79

Prosite motif: PS00715 SIGMA70_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDALAAYSKVNQQTQEALVKTHATLVKRIAHHLLGRLPQSVQLDDLIQSGMLGLLEAARH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
YDSSKGASFETYAGIRIRGHMLDEVRRNDWVPRSVYRNSRMISDAVRILEHKFGREAKDN
CCCCCCCCCHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHH
EIAEELGVSLNDYHDMLQDSISSHLYGFDDLGVTDDVLFDDEGNGSTEPHRNVMRSDMMN
HHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHH
RLTQVIDSLPRKERLVLSLYYEQDLNLKEIGEILEVSESRISQILSQATHRIRSRLPE
HHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MDALAAYSKVNQQTQEALVKTHATLVKRIAHHLLGRLPQSVQLDDLIQSGMLGLLEAARH
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHH
YDSSKGASFETYAGIRIRGHMLDEVRRNDWVPRSVYRNSRMISDAVRILEHKFGREAKDN
CCCCCCCCCHHHCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCHH
EIAEELGVSLNDYHDMLQDSISSHLYGFDDLGVTDDVLFDDEGNGSTEPHRNVMRSDMMN
HHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCEEECCCCCCCCCHHHHHHHHHHHH
RLTQVIDSLPRKERLVLSLYYEQDLNLKEIGEILEVSESRISQILSQATHRIRSRLPE
HHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1560774; 10984043 [H]