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The map label for this gene is fleS [H]

Identifier: 54294650

GI number: 54294650

Start: 1928956

End: 1929987

Strand: Reverse

Name: fleS [H]

Synonym: lpl1727

Alternate gene names: 54294650

Gene position: 1929987-1928956 (Counterclockwise)

Preceding gene: 54294651

Following gene: 54294649

Centisome position: 57.69

GC content: 40.02

Gene sequence:

>1032_bases
ATGAATGACATGCATTATTCAAATTATCAAACTTGTGCAGTCAAACCACAGATAGAATTATCTGAGTGGGACATTTTGTC
CAGTTTGCCTACCGCCTTGGTAATTTTGAATGCCCAGGGCAAAGTGGTGTGGTTAAATCCTTCAGCTGAAGCGATGCTTG
GTTATGGACTGATTGGTTCACTATGGGTAGATGTGATTCAAAAAGCATTTGTTCCAAGAGCTGATGATGGGCATGAGGTG
TCTCTGGCTGATGGAAGGCGTGTGCACGTAGCCATTTCGTCTTTAGAGAGTTTACCTGGTATATTGATTACTTTGTCTGA
CATTACTGCAAGCAGGGATTATGAAGAAGCCAAAGAAAATGAAAAACGATTGGTTTCTATTGGTACAATGACTGCACAAT
TAGCTCATCAGATTAGAACTCCTTTGTCTTCTGCCATATTGTATACCGAGCATTTAAATAATCTCCCAAGTTTGGATCAT
CGCATTAAAAACTGGATTCACAGATTGCAAGAATGTCATGCCAGTATAGAGAAACAAATTCAGGATTTATTATTATTTGC
CAGAGGAACTTCCATAGAGCCAAAGTATGTCGATATGGAGTTTTGGTGCTCTCAATTAATGCAAAGAGCTCAGCCATACA
TCGAGTCCTATGCAGCTGTATTAGATGTTAATAACCATCTTATTGCTCGTGAATCCTCCATACATGGAGAGTCTTTGATT
GGAGCAATTTTAAATCTGATAATCAATGCGCTACAATCTGAGGCAACCCAAATTGATTTAACACTTGCATCCATGGATGA
TTCAGGTATACAAATATCAGTAGAGGATAATGGAAACGGAATGTCTGAAGAAGTCAGGGCACAAGCATTTTCGCCATTCT
ATACAACAAAAGCACAAGGTACCGGCCTTGGATTAGCAGTGGTTTTTGCTGTAGTAAAAGCGCATGGTGGTCGAGTGACT
CTTGAGTCGACTGAAGGGATAGGAACACAGGTAACTATTTATTTACCTTGCCTGAACAGAAACGTAGGGTAA

Upstream 100 bases:

>100_bases
TTCAAAAAAAACTGGTTTGCAATTGGTTAATTAATTGATTTCAAATACATTTTAATTTTGGCCTGAACTTTGCTAGAGAC
ATTTCATAATAATAATTAAA

Downstream 100 bases:

>100_bases
CTTTATCAAACAAAGGATTTTCTATGAGTCATGTTTTAATAGTTGAAGATGATTCAGTATTAAGAGAAGCGTTGGCTGAG
ACTATGACGATCTCAGGCCA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MNDMHYSNYQTCAVKPQIELSEWDILSSLPTALVILNAQGKVVWLNPSAEAMLGYGLIGSLWVDVIQKAFVPRADDGHEV
SLADGRRVHVAISSLESLPGILITLSDITASRDYEEAKENEKRLVSIGTMTAQLAHQIRTPLSSAILYTEHLNNLPSLDH
RIKNWIHRLQECHASIEKQIQDLLLFARGTSIEPKYVDMEFWCSQLMQRAQPYIESYAAVLDVNNHLIARESSIHGESLI
GAILNLIINALQSEATQIDLTLASMDDSGIQISVEDNGNGMSEEVRAQAFSPFYTTKAQGTGLGLAVVFAVVKAHGGRVT
LESTEGIGTQVTIYLPCLNRNVG

Sequences:

>Translated_343_residues
MNDMHYSNYQTCAVKPQIELSEWDILSSLPTALVILNAQGKVVWLNPSAEAMLGYGLIGSLWVDVIQKAFVPRADDGHEV
SLADGRRVHVAISSLESLPGILITLSDITASRDYEEAKENEKRLVSIGTMTAQLAHQIRTPLSSAILYTEHLNNLPSLDH
RIKNWIHRLQECHASIEKQIQDLLLFARGTSIEPKYVDMEFWCSQLMQRAQPYIESYAAVLDVNNHLIARESSIHGESLI
GAILNLIINALQSEATQIDLTLASMDDSGIQISVEDNGNGMSEEVRAQAFSPFYTTKAQGTGLGLAVVFAVVKAHGGRVT
LESTEGIGTQVTIYLPCLNRNVG
>Mature_343_residues
MNDMHYSNYQTCAVKPQIELSEWDILSSLPTALVILNAQGKVVWLNPSAEAMLGYGLIGSLWVDVIQKAFVPRADDGHEV
SLADGRRVHVAISSLESLPGILITLSDITASRDYEEAKENEKRLVSIGTMTAQLAHQIRTPLSSAILYTEHLNNLPSLDH
RIKNWIHRLQECHASIEKQIQDLLLFARGTSIEPKYVDMEFWCSQLMQRAQPYIESYAAVLDVNNHLIARESSIHGESLI
GAILNLIINALQSEATQIDLTLASMDDSGIQISVEDNGNGMSEEVRAQAFSPFYTTKAQGTGLGLAVVFAVVKAHGGRVT
LESTEGIGTQVTIYLPCLNRNVG

Specific function: Member of the two-component regulatory system AtoS/AtoC; may activate AtoC by phosphorylation [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1788549, Length=339, Percent_Identity=29.4985250737463, Blast_Score=112, Evalue=4e-26,
Organism=Escherichia coli, GI1790436, Length=225, Percent_Identity=30.6666666666667, Blast_Score=99, Evalue=3e-22,
Organism=Escherichia coli, GI1790346, Length=230, Percent_Identity=28.2608695652174, Blast_Score=84, Evalue=2e-17,
Organism=Escherichia coli, GI1786600, Length=330, Percent_Identity=25.1515151515152, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1788713, Length=355, Percent_Identity=24.5070422535211, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1789808, Length=213, Percent_Identity=26.2910798122066, Blast_Score=63, Evalue=3e-11,
Organism=Escherichia coli, GI1786783, Length=221, Percent_Identity=25.3393665158371, Blast_Score=63, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR003660
- InterPro:   IPR000014
- InterPro:   IPR000700
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF00672 HAMP; PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 37709; Mature: 37709

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS50112 PAS ; PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNDMHYSNYQTCAVKPQIELSEWDILSSLPTALVILNAQGKVVWLNPSAEAMLGYGLIGS
CCCCCCCCCCEEEECCCCCCHHHHHHHHCCCEEEEEECCCEEEEECCCHHHHHHHHHHHH
LWVDVIQKAFVPRADDGHEVSLADGRRVHVAISSLESLPGILITLSDITASRDYEEAKEN
HHHHHHHHHCCCCCCCCCEEEECCCCEEEEEEHHHHHCCCEEEEEECHHCCCCHHHHHCC
EKRLVSIGTMTAQLAHQIRTPLSSAILYTEHLNNLPSLDHRIKNWIHRLQECHASIEKQI
HHHEEEHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
QDLLLFARGTSIEPKYVDMEFWCSQLMQRAQPYIESYAAVLDVNNHLIARESSIHGESLI
HHHHHHHCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHH
GAILNLIINALQSEATQIDLTLASMDDSGIQISVEDNGNGMSEEVRAQAFSPFYTTKAQG
HHHHHHHHHHHHCCCEEEEEEEEECCCCCEEEEEECCCCCCHHHHHHHHCCCCEECCCCC
TGLGLAVVFAVVKAHGGRVTLESTEGIGTQVTIYLPCLNRNVG
CCHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEEEECCCCCC
>Mature Secondary Structure
MNDMHYSNYQTCAVKPQIELSEWDILSSLPTALVILNAQGKVVWLNPSAEAMLGYGLIGS
CCCCCCCCCCEEEECCCCCCHHHHHHHHCCCEEEEEECCCEEEEECCCHHHHHHHHHHHH
LWVDVIQKAFVPRADDGHEVSLADGRRVHVAISSLESLPGILITLSDITASRDYEEAKEN
HHHHHHHHHCCCCCCCCCEEEECCCCEEEEEEHHHHHCCCEEEEEECHHCCCCHHHHHCC
EKRLVSIGTMTAQLAHQIRTPLSSAILYTEHLNNLPSLDHRIKNWIHRLQECHASIEKQI
HHHEEEHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHH
QDLLLFARGTSIEPKYVDMEFWCSQLMQRAQPYIESYAAVLDVNNHLIARESSIHGESLI
HHHHHHHCCCCCCCCEECHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCCHHHHH
GAILNLIINALQSEATQIDLTLASMDDSGIQISVEDNGNGMSEEVRAQAFSPFYTTKAQG
HHHHHHHHHHHHCCCEEEEEEEEECCCCCEEEEEECCCCCCHHHHHHHHCCCCEECCCCC
TGLGLAVVFAVVKAHGGRVTLESTEGIGTQVTIYLPCLNRNVG
CCHHHHHHHHHHHCCCCEEEEECCCCCCEEEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8346225; 9097040; 9278503 [H]