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The map label for this gene is fliF [H]

Identifier: 54294647

GI number: 54294647

Start: 1925483

End: 1927117

Strand: Reverse

Name: fliF [H]

Synonym: lpl1724

Alternate gene names: 54294647

Gene position: 1927117-1925483 (Counterclockwise)

Preceding gene: 54294648

Following gene: 54294646

Centisome position: 57.6

GC content: 39.94

Gene sequence:

>1635_bases
ATGGCATTGGCTGATAATGCTTCAGTAGCAGCTACAAAATTTGCAAGCTTATCTATACCACGCCAAATTGGAGTGTTGTT
TGGATTGGCAGCCAGTATTGCAATAGGCGTGGCAGTGGTTCTATGGTCTCGTGATCCAAGCTATGTTCCTTTATATAGCC
AGCTCAATGCGCAGGATTCATCAGAGATTTTGACTGTTTTAGAGCGCAATAGTATTGATTTCAAAATTGACAAGAATAAT
GGCTTGTTGATGGTGCCTGCTGATGAGCTGCAAAGTGCGCGTTTGAAATTGGCCGCACAAGGATTGCCTCGCGATACTGC
CATAGGTCATGATCTTTTAGGTAGCAGTGGTGGTGTGTTTAATACCAGTCAATTTATGGAAAACGCCCGATATAAGCAAG
CTCTTGAGGCTGAATTAGCACGTACGATCAGTAAATTTAATAATATTAAATCGGCCAGAGTGCACTTGGCAATTCCAAGA
GAGTCAGCCTTTGTAAGAGACTCACAAGAGCCTAGTGCTTCTGTATTTATTGATGTGTATTCAGGATTTGAAATAAAGAA
ACATACCATTGCAGCAATTGTCAATTTAGTAGCATCAAGTATTCCGAATTTAAGCACAGGAAGAGTAACAGTAGTTGATC
AAGACGGACAATTGCTAAATGAGGGTAGCGGATATAACCTCTTTTCGGAGACCGAGCGTTTTCTTGATTACAGACAAAAC
CTGGAGCATCAATACGCTCAAAAAATTCAGGATATCTTAACCCCAATATTGGGATACGGCCGTGTCAGGGCCAAAGTATC
TGCAGATATTGACTTTACCAGCTATGAACAAACTCAAGAATTGTTCAATCCGGAGCTCTCTGCTTTACGCAGCGAACAAA
CTATGCAAGAGAGTCGTAATGCGTCAAATGAAGCAAGCGGGGTTCCTGGGACATTATCCAATACTCCTCAACCAAATAGT
TCTCTTGGGCAAAAAAATAATCCTCAACAAAATGCAACGCCAAATAACTTACCTCAACAAACATCGTCAGATCAAATGCA
GGCAAAGGATGTTCGCATGCAAACGACTAAAAATTTCGAATTGGATAAAACAATAAGTCATACTAAAAATCAGCCAGGAA
CAATAAAACGCTTGTCGGTTGCGGTATTGGTAGATAATCGACCTGTAATGAATGATAAAACCAAGAAACTGGAGAAGAAA
CCGCTAACTAAAGAAGAAATCGAACAAATAAAGTTATTGGTTTCTGATGCCATAGGATTGAATGTTAAGAGGGGAGATAG
TTTGAATGTGATCAATAGTAGCTTTGTAAAGCCAGATCCTATAGAAGCAATTCCAGAAGAGCATTTTTGGCAAAAAGACA
GTTTTTTGTCTGTGATTAAGCAGATTGGCGGGGCCCTTTTTATATTGGCAATCCTATTTGGTGTATTAAGGCCTATGTTA
AAAACATTGGCAAGCAATAAAGACATGGATCAAGCCACTGATAATCAGGATACAGGAGACTTCACATTAGATGGAAAAAT
ATCATTTAGAGATGTTCAGGATTATGAAACTCAGCTAAGTTTACTTCGTCAAGTGGTTGATAAAGAGCCCAAACGCGTCG
CACAAGTAGTGAAATCATGGGTTGATCGGGGGTAA

Upstream 100 bases:

>100_bases
TTTTTGAATACCATAATGAGTTTGCAAATGTTCTCAATAAAGTTTTAAAGCTCATAGTGAAGTATTCTGGAAAAAAGGAT
AAAAATTACAGGAGTAAATC

Downstream 100 bases:

>100_bases
TTATGGACGGAATAGAGCGAGCAGCTATTTTATTGTTAAGCATGGGAGAAAAAAATGCTTCAGAAGTATTAAAGCATTTA
GAGCCCAGACAAGTACAAAA

Product: flagellar MS-ring protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 544; Mature: 543

Protein sequence:

>544_residues
MALADNASVAATKFASLSIPRQIGVLFGLAASIAIGVAVVLWSRDPSYVPLYSQLNAQDSSEILTVLERNSIDFKIDKNN
GLLMVPADELQSARLKLAAQGLPRDTAIGHDLLGSSGGVFNTSQFMENARYKQALEAELARTISKFNNIKSARVHLAIPR
ESAFVRDSQEPSASVFIDVYSGFEIKKHTIAAIVNLVASSIPNLSTGRVTVVDQDGQLLNEGSGYNLFSETERFLDYRQN
LEHQYAQKIQDILTPILGYGRVRAKVSADIDFTSYEQTQELFNPELSALRSEQTMQESRNASNEASGVPGTLSNTPQPNS
SLGQKNNPQQNATPNNLPQQTSSDQMQAKDVRMQTTKNFELDKTISHTKNQPGTIKRLSVAVLVDNRPVMNDKTKKLEKK
PLTKEEIEQIKLLVSDAIGLNVKRGDSLNVINSSFVKPDPIEAIPEEHFWQKDSFLSVIKQIGGALFILAILFGVLRPML
KTLASNKDMDQATDNQDTGDFTLDGKISFRDVQDYETQLSLLRQVVDKEPKRVAQVVKSWVDRG

Sequences:

>Translated_544_residues
MALADNASVAATKFASLSIPRQIGVLFGLAASIAIGVAVVLWSRDPSYVPLYSQLNAQDSSEILTVLERNSIDFKIDKNN
GLLMVPADELQSARLKLAAQGLPRDTAIGHDLLGSSGGVFNTSQFMENARYKQALEAELARTISKFNNIKSARVHLAIPR
ESAFVRDSQEPSASVFIDVYSGFEIKKHTIAAIVNLVASSIPNLSTGRVTVVDQDGQLLNEGSGYNLFSETERFLDYRQN
LEHQYAQKIQDILTPILGYGRVRAKVSADIDFTSYEQTQELFNPELSALRSEQTMQESRNASNEASGVPGTLSNTPQPNS
SLGQKNNPQQNATPNNLPQQTSSDQMQAKDVRMQTTKNFELDKTISHTKNQPGTIKRLSVAVLVDNRPVMNDKTKKLEKK
PLTKEEIEQIKLLVSDAIGLNVKRGDSLNVINSSFVKPDPIEAIPEEHFWQKDSFLSVIKQIGGALFILAILFGVLRPML
KTLASNKDMDQATDNQDTGDFTLDGKISFRDVQDYETQLSLLRQVVDKEPKRVAQVVKSWVDRG
>Mature_543_residues
ALADNASVAATKFASLSIPRQIGVLFGLAASIAIGVAVVLWSRDPSYVPLYSQLNAQDSSEILTVLERNSIDFKIDKNNG
LLMVPADELQSARLKLAAQGLPRDTAIGHDLLGSSGGVFNTSQFMENARYKQALEAELARTISKFNNIKSARVHLAIPRE
SAFVRDSQEPSASVFIDVYSGFEIKKHTIAAIVNLVASSIPNLSTGRVTVVDQDGQLLNEGSGYNLFSETERFLDYRQNL
EHQYAQKIQDILTPILGYGRVRAKVSADIDFTSYEQTQELFNPELSALRSEQTMQESRNASNEASGVPGTLSNTPQPNSS
LGQKNNPQQNATPNNLPQQTSSDQMQAKDVRMQTTKNFELDKTISHTKNQPGTIKRLSVAVLVDNRPVMNDKTKKLEKKP
LTKEEIEQIKLLVSDAIGLNVKRGDSLNVINSSFVKPDPIEAIPEEHFWQKDSFLSVIKQIGGALFILAILFGVLRPMLK
TLASNKDMDQATDNQDTGDFTLDGKISFRDVQDYETQLSLLRQVVDKEPKRVAQVVKSWVDRG

Specific function: The M ring may be actively involved in energy transduction [H]

COG id: COG1766

COG function: function code NU; Flagellar biosynthesis/type III secretory pathway lipoprotein

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein. Bacterial flagellum basal body (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the fliF family [H]

Homologues:

Organism=Escherichia coli, GI1788248, Length=569, Percent_Identity=34.7978910369069, Blast_Score=302, Evalue=4e-83,

Paralogues:

None

Copy number: 10-20 (rich media) [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013556
- InterPro:   IPR000067
- InterPro:   IPR006182 [H]

Pfam domain/function: PF01514 YscJ_FliF; PF08345 YscJ_FliF_C [H]

EC number: NA

Molecular weight: Translated: 60096; Mature: 59964

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALADNASVAATKFASLSIPRQIGVLFGLAASIAIGVAVVLWSRDPSYVPLYSQLNAQDS
CCCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHCCCCCH
SEILTVLERNSIDFKIDKNNGLLMVPADELQSARLKLAAQGLPRDTAIGHDLLGSSGGVF
HHHHHHHHCCCCEEEEECCCCEEEEEHHHHHHHHHHHHHCCCCCCHHHCHHHHCCCCCCC
NTSQFMENARYKQALEAELARTISKFNNIKSARVHLAIPRESAFVRDSQEPSASVFIDVY
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHCCCCCCCCCEEEEEEC
SGFEIKKHTIAAIVNLVASSIPNLSTGRVTVVDQDGQLLNEGSGYNLFSETERFLDYRQN
CCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHCCCCCCCHHHHHHHHHHHHHH
LEHQYAQKIQDILTPILGYGRVRAKVSADIDFTSYEQTQELFNPELSALRSEQTMQESRN
HHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHCC
ASNEASGVPGTLSNTPQPNSSLGQKNNPQQNATPNNLPQQTSSDQMQAKDVRMQTTKNFE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCC
LDKTISHTKNQPGTIKRLSVAVLVDNRPVMNDKTKKLEKKPLTKEEIEQIKLLVSDAIGL
HHHHHHHCCCCCCCEEEEEEEEEECCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHCC
NVKRGDSLNVINSSFVKPDPIEAIPEEHFWQKDSFLSVIKQIGGALFILAILFGVLRPML
EECCCCCCCHHHCCCCCCCCHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KTLASNKDMDQATDNQDTGDFTLDGKISFRDVQDYETQLSLLRQVVDKEPKRVAQVVKSW
HHHHCCCCCCCCCCCCCCCCEEECCEEEECHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
VDRG
HCCC
>Mature Secondary Structure 
ALADNASVAATKFASLSIPRQIGVLFGLAASIAIGVAVVLWSRDPSYVPLYSQLNAQDS
CCCCCCCHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCCCHHHHCCCCCH
SEILTVLERNSIDFKIDKNNGLLMVPADELQSARLKLAAQGLPRDTAIGHDLLGSSGGVF
HHHHHHHHCCCCEEEEECCCCEEEEEHHHHHHHHHHHHHCCCCCCHHHCHHHHCCCCCCC
NTSQFMENARYKQALEAELARTISKFNNIKSARVHLAIPRESAFVRDSQEPSASVFIDVY
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHCCCCCCCCCEEEEEEC
SGFEIKKHTIAAIVNLVASSIPNLSTGRVTVVDQDGQLLNEGSGYNLFSETERFLDYRQN
CCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHCCCCCCCHHHHHHHHHHHHHH
LEHQYAQKIQDILTPILGYGRVRAKVSADIDFTSYEQTQELFNPELSALRSEQTMQESRN
HHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHCHHHHHHHHHHHHHHHCC
ASNEASGVPGTLSNTPQPNSSLGQKNNPQQNATPNNLPQQTSSDQMQAKDVRMQTTKNFE
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCC
LDKTISHTKNQPGTIKRLSVAVLVDNRPVMNDKTKKLEKKPLTKEEIEQIKLLVSDAIGL
HHHHHHHCCCCCCCEEEEEEEEEECCCCCCCCHHHHHHHCCCCHHHHHHHHHHHHHHHCC
NVKRGDSLNVINSSFVKPDPIEAIPEEHFWQKDSFLSVIKQIGGALFILAILFGVLRPML
EECCCCCCCHHHCCCCCCCCHHHCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KTLASNKDMDQATDNQDTGDFTLDGKISFRDVQDYETQLSLLRQVVDKEPKRVAQVVKSW
HHHHCCCCCCCCCCCCCCCCEEECCEEEECHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
VDRG
HCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8675317; 10984043 [H]