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The map label for this gene is mltD [H]

Identifier: 54294192

GI number: 54294192

Start: 1397843

End: 1399282

Strand: Reverse

Name: mltD [H]

Synonym: lpl1256

Alternate gene names: 54294192

Gene position: 1399282-1397843 (Counterclockwise)

Preceding gene: 54294193

Following gene: 54294191

Centisome position: 41.82

GC content: 38.33

Gene sequence:

>1440_bases
TTGAATTGTAATTTATTGATTATAAAAATATTTTTTTCATTCATCCTTATTTTTATTGGTATTTCTAACCCGGCAATTTC
TGGCACAGCTCCAGATGTATGGGATGTATTACGTAGTGAATTTACCTTAAGCCATGAAGTAACTCGCCCAGAAGTGCAAG
AACAAATCAGATGGCTAATTTCTCATCCCAGTTATATCAACAAAGTAAGTAGCCAATCTGAACCTTACATTTACCACGTG
TTAGGTGAGATAAAGAAAAGAAAACTGCCCGGAGAATTGGCGCTACTCCCCATGATAGAAAGTGCATATGATCCTTTTGC
CTATTCAGGAGCAGGCGCTGCTGGTATATGGCAACTCATGCCTCGAACTGGAAGCGATCTTGGATTAAAGCAAGACTGGT
GGTTTGATGGAAGACGTAGCATTGGCCCTTCAACTGATGCCGCATTAAATTACCTCGTCTATCTAAATAAATATTTTAGT
GGTAACTGGATTTTAGCTATTGCCGCTTATGACTCTGGGGAAGGAACCATTTCACGGGCGCTCAAATCAACCGCACGTAA
CGGGCGCAAGGGGCAATTCTGGAATCTGCCAGTTCCCAGAGAAACCAAGGTATATATCCCGCGTTTATTAGCTCTGGCAG
AAATTATTAACAATCCAAAACGGTATAAAATATCGCTTCCCAATATTCCCTATCTTCCTTATTTTGAAGAGGTCAATATT
GGTAGTCAAATTGATTTAAATCATGCTGCTAAACTCGCCGGCATCAGTTATAAGGAGTTGATTAAACTAAATCCCGGTTA
TAATCGATGGACAACGGCACCTTATAAGCCATTTAAATTACTAATACCTGCTGAAAAAGTCGAAAGATTTAATTTGAATC
TATCGAATATACCCGAAGATAAGCGCGTAAACTGGACTAAACATCAAGTAAAAAGAGGTGACAGCTTGGATGCTATTGCA
AAGAGATATCACACAACAGTCAATTTGATAAAACAACTCAATCAATTAACCAGCAATAAAGTTCAACTTAATCAATCCCT
ACTTATTCCGAGCAGCAAAAATTCCCCAGTAGCAGCTAAAATACCTCCACAGGAAAAAAAACCTGCTACTAAACAGCCGG
CACCTATGAAAAATGTCCGTATTATTCATATCGTACAAAATAATGATAGCTATCAGAAAATTCAAAAAGCTTATGGGGTT
GCGGCTAAAGAGATAAAAAACTGGAATAAATTGGCAAATAACGCGCCATTACGTAAAGGCCAGCGGCTGGTCATATGGAA
AAGAGTCAAGCAACCTGTTGTGTATATAGTAAAATCAGGGGATAGTTTGAGCGTTATTGCCAAAAAGCACCAAACAAAAG
TGAATACTATTATCAGTTTAAATCCAGGACTTAAAAAATCAATGCCCCTTCGCTTGGGACAAAAAATATTAATTGGCTAG

Upstream 100 bases:

>100_bases
CAAATAAAAATCCATTTGCACCAAATTAAACCAAAAAGGGTTTAATCCTTCATTAATTTTTAGTAAACTGATTAGCCAAT
TATAATAACAGGTAAGACCA

Downstream 100 bases:

>100_bases
ATTATGTGCATTATCATGATATAAACTAGATAGATTAATACATCTGTTTGAAACAGAAACGTGCTTTTGAGGGAATACCA
AGACTACTGGCATGACGCTA

Product: hypothetical protein

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase D; Regulatory protein dniR [H]

Number of amino acids: Translated: 479; Mature: 479

Protein sequence:

>479_residues
MNCNLLIIKIFFSFILIFIGISNPAISGTAPDVWDVLRSEFTLSHEVTRPEVQEQIRWLISHPSYINKVSSQSEPYIYHV
LGEIKKRKLPGELALLPMIESAYDPFAYSGAGAAGIWQLMPRTGSDLGLKQDWWFDGRRSIGPSTDAALNYLVYLNKYFS
GNWILAIAAYDSGEGTISRALKSTARNGRKGQFWNLPVPRETKVYIPRLLALAEIINNPKRYKISLPNIPYLPYFEEVNI
GSQIDLNHAAKLAGISYKELIKLNPGYNRWTTAPYKPFKLLIPAEKVERFNLNLSNIPEDKRVNWTKHQVKRGDSLDAIA
KRYHTTVNLIKQLNQLTSNKVQLNQSLLIPSSKNSPVAAKIPPQEKKPATKQPAPMKNVRIIHIVQNNDSYQKIQKAYGV
AAKEIKNWNKLANNAPLRKGQRLVIWKRVKQPVVYIVKSGDSLSVIAKKHQTKVNTIISLNPGLKKSMPLRLGQKILIG

Sequences:

>Translated_479_residues
MNCNLLIIKIFFSFILIFIGISNPAISGTAPDVWDVLRSEFTLSHEVTRPEVQEQIRWLISHPSYINKVSSQSEPYIYHV
LGEIKKRKLPGELALLPMIESAYDPFAYSGAGAAGIWQLMPRTGSDLGLKQDWWFDGRRSIGPSTDAALNYLVYLNKYFS
GNWILAIAAYDSGEGTISRALKSTARNGRKGQFWNLPVPRETKVYIPRLLALAEIINNPKRYKISLPNIPYLPYFEEVNI
GSQIDLNHAAKLAGISYKELIKLNPGYNRWTTAPYKPFKLLIPAEKVERFNLNLSNIPEDKRVNWTKHQVKRGDSLDAIA
KRYHTTVNLIKQLNQLTSNKVQLNQSLLIPSSKNSPVAAKIPPQEKKPATKQPAPMKNVRIIHIVQNNDSYQKIQKAYGV
AAKEIKNWNKLANNAPLRKGQRLVIWKRVKQPVVYIVKSGDSLSVIAKKHQTKVNTIISLNPGLKKSMPLRLGQKILIG
>Mature_479_residues
MNCNLLIIKIFFSFILIFIGISNPAISGTAPDVWDVLRSEFTLSHEVTRPEVQEQIRWLISHPSYINKVSSQSEPYIYHV
LGEIKKRKLPGELALLPMIESAYDPFAYSGAGAAGIWQLMPRTGSDLGLKQDWWFDGRRSIGPSTDAALNYLVYLNKYFS
GNWILAIAAYDSGEGTISRALKSTARNGRKGQFWNLPVPRETKVYIPRLLALAEIINNPKRYKISLPNIPYLPYFEEVNI
GSQIDLNHAAKLAGISYKELIKLNPGYNRWTTAPYKPFKLLIPAEKVERFNLNLSNIPEDKRVNWTKHQVKRGDSLDAIA
KRYHTTVNLIKQLNQLTSNKVQLNQSLLIPSSKNSPVAAKIPPQEKKPATKQPAPMKNVRIIHIVQNNDSYQKIQKAYGV
AAKEIKNWNKLANNAPLRKGQRLVIWKRVKQPVVYIVKSGDSLSVIAKKHQTKVNTIISLNPGLKKSMPLRLGQKILIG

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1786405, Length=408, Percent_Identity=33.8235294117647, Blast_Score=251, Evalue=7e-68,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR010511
- InterPro:   IPR018392
- InterPro:   IPR002482
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01476 LysM; PF06474 MLTD_N; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 54147; Mature: 54147

Theoretical pI: Translated: 10.60; Mature: 10.60

Prosite motif: PS00922 TRANSGLYCOSYLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNCNLLIIKIFFSFILIFIGISNPAISGTAPDVWDVLRSEFTLSHEVTRPEVQEQIRWLI
CCCCEEEHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
SHPSYINKVSSQSEPYIYHVLGEIKKRKLPGELALLPMIESAYDPFAYSGAGAAGIWQLM
CCCHHHHHHCCCCCCEEEEHHHHHHHHCCCCCEEEHHHHHHCCCCCCCCCCCHHHHHHHC
PRTGSDLGLKQDWWFDGRRSIGPSTDAALNYLVYLNKYFSGNWILAIAAYDSGEGTISRA
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHEECCCEEEEEEEECCCCHHHHHH
LKSTARNGRKGQFWNLPVPRETKVYIPRLLALAEIINNPKRYKISLPNIPYLPYFEEVNI
HHHHHCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHCCC
GSQIDLNHAAKLAGISYKELIKLNPGYNRWTTAPYKPFKLLIPAEKVERFNLNLSNIPED
CCEECCCHHHHHCCCCHHHHEEECCCCCCCCCCCCCCEEEEECHHHHHHCCCCHHCCCCC
KRVNWTKHQVKRGDSLDAIAKRYHTTVNLIKQLNQLTSNKVQLNQSLLIPSSKNSPVAAK
CCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECCEEECCCCCCCCEEEE
IPPQEKKPATKQPAPMKNVRIIHIVQNNDSYQKIQKAYGVAAKEIKNWNKLANNAPLRKG
CCCCCCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
QRLVIWKRVKQPVVYIVKSGDSLSVIAKKHQTKVNTIISLNPGLKKSMPLRLGQKILIG
CEEEEEHHCCCCEEEEEECCCCEEEHHHHHCCEEEEEEEECCCCCCCCCHHCCCEECCC
>Mature Secondary Structure
MNCNLLIIKIFFSFILIFIGISNPAISGTAPDVWDVLRSEFTLSHEVTRPEVQEQIRWLI
CCCCEEEHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHHH
SHPSYINKVSSQSEPYIYHVLGEIKKRKLPGELALLPMIESAYDPFAYSGAGAAGIWQLM
CCCHHHHHHCCCCCCEEEEHHHHHHHHCCCCCEEEHHHHHHCCCCCCCCCCCHHHHHHHC
PRTGSDLGLKQDWWFDGRRSIGPSTDAALNYLVYLNKYFSGNWILAIAAYDSGEGTISRA
CCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHEECCCEEEEEEEECCCCHHHHHH
LKSTARNGRKGQFWNLPVPRETKVYIPRLLALAEIINNPKRYKISLPNIPYLPYFEEVNI
HHHHHCCCCCCCEECCCCCCCCHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCHHHCCC
GSQIDLNHAAKLAGISYKELIKLNPGYNRWTTAPYKPFKLLIPAEKVERFNLNLSNIPED
CCEECCCHHHHHCCCCHHHHEEECCCCCCCCCCCCCCEEEEECHHHHHHCCCCHHCCCCC
KRVNWTKHQVKRGDSLDAIAKRYHTTVNLIKQLNQLTSNKVQLNQSLLIPSSKNSPVAAK
CCCCCHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEECCEEECCCCCCCCEEEE
IPPQEKKPATKQPAPMKNVRIIHIVQNNDSYQKIQKAYGVAAKEIKNWNKLANNAPLRKG
CCCCCCCCCCCCCCCCCCEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
QRLVIWKRVKQPVVYIVKSGDSLSVIAKKHQTKVNTIISLNPGLKKSMPLRLGQKILIG
CEEEEEHHCCCCEEEEEECCCCEEEHHHHHCCEEEEEEEECCCCCCCCCHHCCCEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]