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The map label for this gene is lcfA [H]

Identifier: 54294069

GI number: 54294069

Start: 1263087

End: 1264544

Strand: Reverse

Name: lcfA [H]

Synonym: lpl1133

Alternate gene names: 54294069

Gene position: 1264544-1263087 (Counterclockwise)

Preceding gene: 54294071

Following gene: 54294065

Centisome position: 37.8

GC content: 38.68

Gene sequence:

>1458_bases
ATGAAAAAATGCTCTATGGTTATTGCAGGGCAACCCATAACCTATGAGAGATTCTTCGTTACAGTGAATCGTATTGCAAC
TCACTTTCAAAATAAAGTATCCTCTGGAAGCCGTATCGCTTTTATGTTGCCCAATAGTCTTGAAATCATAGCCATTTACC
TGGCCTGTTTTCAATCAGGATGTATTGCCATGCCTGTTAATCGCCGCTATGCGCCACCTGAGCTTGAAAAGGTATTACAA
GATGCCCAACCAATTTATTTAATTATCGAAGCAAGTAAATTATTTTTGCTAGAGAAAATCAATTGGTCAGCAACAGGAAT
TAAAAAAATTTTTGTGCTAGGTGAAGACCGAAGTCATCCCTATTTTTCTTTTGATGACTTGTTGACTGAACCAGAAAAAT
TGCCAAAACAATCCATATCGTATGAAACTCCCGCGGTCATCTTCTATACGTCTGGTTCAACCGGGCAGCCTAAAGGAGTT
GTACATACACTTTCTTCCATTGCAGCGATGCTTGACTCGACTTCATTGACACTGGAAAACATTACCGCTGACGACAAAAT
GATAATCTGTGAGCCACAATGCCATGTAAGTGGATTTATCGAAACATTCTCTACATTATCACGAGAAGGTACCGCGTTAG
TTCATGATGGTTTTGATATGGATTGGTACCTTGCGACAGTGCGACATGAAAAACCGACACTCGCCGTACATCATATTGAT
ACCTATATAAAGTTATTAGACTCTGGACGCTGTAATACAAAAACATTTCAATCATTTCGTGGAATTTATACAGGTGGGGA
TGCTCTACCCGTGGCAGTCCAGGACAAGTTTCTGGCTTGCGCTGGTAAACCAATTCAAGTGGGTTATGGAATGACGGAAG
CCATTTGGCTAACAGTCTGCAGAACCCCTGATTTGACTCAAAAATCCTGTATTGGCAAACCGGTGAATGGGGTAACATTA
CGCCTTGTAAACGCAGCTGGAGAAGACGTACGTTGTGGTGAAACAGGAGAAATACTGGTAAAAGGTGATATGGTGATGCA
TAGTTATTGGAATAATCCTGAAGAAACCCGCAAGGCATTTATAAAAGGTTGGTTTAAAACAGGAGATTGCGGAATTCAGG
ATGAACAAGGTTATTTTTATTATAAAGGTCGGATTAAGAACATTATTATCCGTAATACATCCAATATTATGCCTGGTGAA
GTGGAAGCAGCCATTTATAAACATCCAGCAATTAGCGCAGCGGCTGTGATTGGTGTACCTGACGAAGCAGAAGGAGAAGT
ACCAATTGCTTTTGTAGTAGTAAAAAAGAGTAACCAATTGACAAAAGAAGAATTATATTCTTTTTTAATCGAGCAAATTG
CTCAATATAAAATACCTGAAAAAATTTATTTTATCGATGAGATGCCCTTGACTAATAGCGGAAAAATTAATCATAAAAAA
TTGTATGATTATCTTTAA

Upstream 100 bases:

>100_bases
ATTTCCCATAATATAAAAAATAACAAATGAATCTATCCAGGAAAACATAATGACTAAAAAAAGAATAGACAGGCTTATTT
TTTCAGAAGTTGAATGCAAT

Downstream 100 bases:

>100_bases
AAATAATCGAAATTGCATCAAATGACTCACACTTCATGGTGATTTTCCATACCGATTCAAAGCATAATGTCCCATACTTT
TTGCTATTTCTCCCTCAGCA

Product: hypothetical protein

Products: NA

Alternate protein names: Long-chain acyl-CoA synthetase [H]

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MKKCSMVIAGQPITYERFFVTVNRIATHFQNKVSSGSRIAFMLPNSLEIIAIYLACFQSGCIAMPVNRRYAPPELEKVLQ
DAQPIYLIIEASKLFLLEKINWSATGIKKIFVLGEDRSHPYFSFDDLLTEPEKLPKQSISYETPAVIFYTSGSTGQPKGV
VHTLSSIAAMLDSTSLTLENITADDKMIICEPQCHVSGFIETFSTLSREGTALVHDGFDMDWYLATVRHEKPTLAVHHID
TYIKLLDSGRCNTKTFQSFRGIYTGGDALPVAVQDKFLACAGKPIQVGYGMTEAIWLTVCRTPDLTQKSCIGKPVNGVTL
RLVNAAGEDVRCGETGEILVKGDMVMHSYWNNPEETRKAFIKGWFKTGDCGIQDEQGYFYYKGRIKNIIIRNTSNIMPGE
VEAAIYKHPAISAAAVIGVPDEAEGEVPIAFVVVKKSNQLTKEELYSFLIEQIAQYKIPEKIYFIDEMPLTNSGKINHKK
LYDYL

Sequences:

>Translated_485_residues
MKKCSMVIAGQPITYERFFVTVNRIATHFQNKVSSGSRIAFMLPNSLEIIAIYLACFQSGCIAMPVNRRYAPPELEKVLQ
DAQPIYLIIEASKLFLLEKINWSATGIKKIFVLGEDRSHPYFSFDDLLTEPEKLPKQSISYETPAVIFYTSGSTGQPKGV
VHTLSSIAAMLDSTSLTLENITADDKMIICEPQCHVSGFIETFSTLSREGTALVHDGFDMDWYLATVRHEKPTLAVHHID
TYIKLLDSGRCNTKTFQSFRGIYTGGDALPVAVQDKFLACAGKPIQVGYGMTEAIWLTVCRTPDLTQKSCIGKPVNGVTL
RLVNAAGEDVRCGETGEILVKGDMVMHSYWNNPEETRKAFIKGWFKTGDCGIQDEQGYFYYKGRIKNIIIRNTSNIMPGE
VEAAIYKHPAISAAAVIGVPDEAEGEVPIAFVVVKKSNQLTKEELYSFLIEQIAQYKIPEKIYFIDEMPLTNSGKINHKK
LYDYL
>Mature_485_residues
MKKCSMVIAGQPITYERFFVTVNRIATHFQNKVSSGSRIAFMLPNSLEIIAIYLACFQSGCIAMPVNRRYAPPELEKVLQ
DAQPIYLIIEASKLFLLEKINWSATGIKKIFVLGEDRSHPYFSFDDLLTEPEKLPKQSISYETPAVIFYTSGSTGQPKGV
VHTLSSIAAMLDSTSLTLENITADDKMIICEPQCHVSGFIETFSTLSREGTALVHDGFDMDWYLATVRHEKPTLAVHHID
TYIKLLDSGRCNTKTFQSFRGIYTGGDALPVAVQDKFLACAGKPIQVGYGMTEAIWLTVCRTPDLTQKSCIGKPVNGVTL
RLVNAAGEDVRCGETGEILVKGDMVMHSYWNNPEETRKAFIKGWFKTGDCGIQDEQGYFYYKGRIKNIIIRNTSNIMPGE
VEAAIYKHPAISAAAVIGVPDEAEGEVPIAFVVVKKSNQLTKEELYSFLIEQIAQYKIPEKIYFIDEMPLTNSGKINHKK
LYDYL

Specific function: Esterification, Concomitant With Transport, Of Exogenous Long-Chain Fatty Acids Into Metabolically Active CoA Thioesters For Subsequent Degradation Or Incorporation Into Phospholipids. [C]

COG id: COG0318

COG function: function code IQ; Acyl-CoA synthetases (AMP-forming)/AMP-acid ligases II

Gene ontology:

Cell location: Partially Membrane-Associated [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ATP-dependent AMP-binding enzyme family [H]

Homologues:

Organism=Homo sapiens, GI187761345, Length=493, Percent_Identity=27.5862068965517, Blast_Score=160, Evalue=3e-39,
Organism=Homo sapiens, GI187761343, Length=493, Percent_Identity=27.5862068965517, Blast_Score=160, Evalue=3e-39,
Organism=Homo sapiens, GI38505220, Length=461, Percent_Identity=26.8980477223427, Blast_Score=153, Evalue=5e-37,
Organism=Homo sapiens, GI156151445, Length=526, Percent_Identity=27.1863117870722, Blast_Score=145, Evalue=1e-34,
Organism=Homo sapiens, GI122937307, Length=465, Percent_Identity=25.3763440860215, Blast_Score=138, Evalue=1e-32,
Organism=Homo sapiens, GI42544132, Length=465, Percent_Identity=24.0860215053763, Blast_Score=132, Evalue=1e-30,
Organism=Homo sapiens, GI157311624, Length=468, Percent_Identity=25.8547008547009, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI157311622, Length=468, Percent_Identity=25.8547008547009, Blast_Score=129, Evalue=6e-30,
Organism=Homo sapiens, GI58082049, Length=468, Percent_Identity=25.6410256410256, Blast_Score=125, Evalue=6e-29,
Organism=Homo sapiens, GI115511026, Length=477, Percent_Identity=24.9475890985325, Blast_Score=125, Evalue=1e-28,
Organism=Homo sapiens, GI13375727, Length=529, Percent_Identity=21.5500945179584, Blast_Score=100, Evalue=5e-21,
Organism=Homo sapiens, GI28416953, Length=122, Percent_Identity=36.0655737704918, Blast_Score=87, Evalue=4e-17,
Organism=Homo sapiens, GI253970489, Length=361, Percent_Identity=26.0387811634349, Blast_Score=82, Evalue=9e-16,
Organism=Homo sapiens, GI8923896, Length=361, Percent_Identity=26.0387811634349, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI1788107, Length=524, Percent_Identity=27.0992366412214, Blast_Score=178, Evalue=7e-46,
Organism=Escherichia coli, GI145693145, Length=468, Percent_Identity=28.8461538461538, Blast_Score=159, Evalue=4e-40,
Organism=Escherichia coli, GI221142682, Length=483, Percent_Identity=26.2939958592132, Blast_Score=132, Evalue=5e-32,
Organism=Escherichia coli, GI1786810, Length=252, Percent_Identity=28.5714285714286, Blast_Score=105, Evalue=8e-24,
Organism=Escherichia coli, GI1789201, Length=502, Percent_Identity=24.1035856573705, Blast_Score=101, Evalue=8e-23,
Organism=Escherichia coli, GI1786529, Length=518, Percent_Identity=22.5868725868726, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1790505, Length=518, Percent_Identity=22.007722007722, Blast_Score=93, Evalue=5e-20,
Organism=Escherichia coli, GI1788595, Length=337, Percent_Identity=25.2225519287834, Blast_Score=83, Evalue=3e-17,
Organism=Escherichia coli, GI1786801, Length=489, Percent_Identity=23.3128834355828, Blast_Score=80, Evalue=3e-16,
Organism=Caenorhabditis elegans, GI17558820, Length=479, Percent_Identity=28.8100208768267, Blast_Score=177, Evalue=9e-45,
Organism=Caenorhabditis elegans, GI32563687, Length=484, Percent_Identity=28.099173553719, Blast_Score=166, Evalue=2e-41,
Organism=Caenorhabditis elegans, GI17557194, Length=502, Percent_Identity=26.6932270916335, Blast_Score=149, Evalue=5e-36,
Organism=Caenorhabditis elegans, GI17538037, Length=489, Percent_Identity=27.6073619631902, Blast_Score=144, Evalue=1e-34,
Organism=Caenorhabditis elegans, GI17559526, Length=496, Percent_Identity=26.2096774193548, Blast_Score=142, Evalue=4e-34,
Organism=Caenorhabditis elegans, GI17531443, Length=453, Percent_Identity=25.1655629139073, Blast_Score=141, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI71994703, Length=517, Percent_Identity=26.8858800773694, Blast_Score=139, Evalue=4e-33,
Organism=Caenorhabditis elegans, GI71994694, Length=517, Percent_Identity=26.8858800773694, Blast_Score=139, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI71994690, Length=517, Percent_Identity=26.8858800773694, Blast_Score=138, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI17560140, Length=463, Percent_Identity=24.1900647948164, Blast_Score=122, Evalue=3e-28,
Organism=Caenorhabditis elegans, GI17560308, Length=517, Percent_Identity=24.1779497098646, Blast_Score=122, Evalue=6e-28,
Organism=Caenorhabditis elegans, GI71985884, Length=369, Percent_Identity=27.9132791327913, Blast_Score=111, Evalue=9e-25,
Organism=Caenorhabditis elegans, GI71996755, Length=499, Percent_Identity=24.4488977955912, Blast_Score=106, Evalue=3e-23,
Organism=Caenorhabditis elegans, GI71983001, Length=379, Percent_Identity=24.0105540897098, Blast_Score=77, Evalue=2e-14,
Organism=Caenorhabditis elegans, GI71982997, Length=379, Percent_Identity=24.0105540897098, Blast_Score=77, Evalue=2e-14,
Organism=Saccharomyces cerevisiae, GI6319699, Length=516, Percent_Identity=27.5193798449612, Blast_Score=164, Evalue=4e-41,
Organism=Saccharomyces cerevisiae, GI6319264, Length=528, Percent_Identity=24.0530303030303, Blast_Score=91, Evalue=6e-19,
Organism=Saccharomyces cerevisiae, GI6323182, Length=194, Percent_Identity=29.8969072164948, Blast_Score=84, Evalue=6e-17,
Organism=Saccharomyces cerevisiae, GI6323903, Length=144, Percent_Identity=33.3333333333333, Blast_Score=68, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6322182, Length=130, Percent_Identity=33.0769230769231, Blast_Score=64, Evalue=5e-11,
Organism=Drosophila melanogaster, GI21355181, Length=506, Percent_Identity=27.0750988142292, Blast_Score=145, Evalue=8e-35,
Organism=Drosophila melanogaster, GI18859661, Length=472, Percent_Identity=27.3305084745763, Blast_Score=136, Evalue=3e-32,
Organism=Drosophila melanogaster, GI24581924, Length=513, Percent_Identity=24.3664717348928, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI21356947, Length=487, Percent_Identity=24.435318275154, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI24653035, Length=489, Percent_Identity=24.1308793456033, Blast_Score=123, Evalue=3e-28,
Organism=Drosophila melanogaster, GI19922652, Length=490, Percent_Identity=24.2857142857143, Blast_Score=122, Evalue=6e-28,
Organism=Drosophila melanogaster, GI21356441, Length=452, Percent_Identity=25, Blast_Score=118, Evalue=9e-27,
Organism=Drosophila melanogaster, GI24648260, Length=343, Percent_Identity=26.530612244898, Blast_Score=111, Evalue=1e-24,
Organism=Drosophila melanogaster, GI161076582, Length=488, Percent_Identity=23.3606557377049, Blast_Score=110, Evalue=3e-24,
Organism=Drosophila melanogaster, GI21358303, Length=422, Percent_Identity=23.9336492890995, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24648253, Length=485, Percent_Identity=22.680412371134, Blast_Score=102, Evalue=5e-22,
Organism=Drosophila melanogaster, GI24656500, Length=495, Percent_Identity=20.8080808080808, Blast_Score=102, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24648255, Length=485, Percent_Identity=22.680412371134, Blast_Score=101, Evalue=1e-21,
Organism=Drosophila melanogaster, GI20130357, Length=483, Percent_Identity=22.1532091097308, Blast_Score=96, Evalue=7e-20,
Organism=Drosophila melanogaster, GI281365686, Length=490, Percent_Identity=21.4285714285714, Blast_Score=87, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21355225, Length=523, Percent_Identity=21.7973231357553, Blast_Score=86, Evalue=4e-17,
Organism=Drosophila melanogaster, GI24648257, Length=460, Percent_Identity=21.7391304347826, Blast_Score=83, Evalue=5e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020845
- InterPro:   IPR000873 [H]

Pfam domain/function: PF00501 AMP-binding [H]

EC number: =6.2.1.3 [H]

Molecular weight: Translated: 54187; Mature: 54187

Theoretical pI: Translated: 6.73; Mature: 6.73

Prosite motif: PS00455 AMP_BINDING

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKKCSMVIAGQPITYERFFVTVNRIATHFQNKVSSGSRIAFMLPNSLEIIAIYLACFQSG
CCCCEEEEECCCCEEHHHEEEHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHCC
CIAMPVNRRYAPPELEKVLQDAQPIYLIIEASKLFLLEKINWSATGIKKIFVLGEDRSHP
CEEECCCCCCCCHHHHHHHHCCCCEEEEEECCEEEEEEECCCCCCCEEEEEEEECCCCCC
YFSFDDLLTEPEKLPKQSISYETPAVIFYTSGSTGQPKGVVHTLSSIAAMLDSTSLTLEN
CCCHHHHHCCHHHCCHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEE
ITADDKMIICEPQCHVSGFIETFSTLSREGTALVHDGFDMDWYLATVRHEKPTLAVHHID
CCCCCCEEEECCCHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEEEEECCCCEEEEEHHH
TYIKLLDSGRCNTKTFQSFRGIYTGGDALPVAVQDKFLACAGKPIQVGYGMTEAIWLTVC
HHHHHHCCCCCCHHHHHHHCCEEECCCEEEEEECCCEEEECCCEEEECCCCCEEEEEEEE
RTPDLTQKSCIGKPVNGVTLRLVNAAGEDVRCGETGEILVKGDMVMHSYWNNPEETRKAF
CCCCCCHHHHCCCCCCCEEEEEEECCCCCEECCCCCCEEEECCEEEEECCCCHHHHHHHH
IKGWFKTGDCGIQDEQGYFYYKGRIKNIIIRNTSNIMPGEVEAAIYKHPAISAAAVIGVP
HHHCCCCCCCCCCCCCCEEEECCEEEEEEEECCCCCCCCCEEEEEECCCCCCEEEEEECC
DEAEGEVPIAFVVVKKSNQLTKEELYSFLIEQIAQYKIPEKIYFIDEMPLTNSGKINHKK
CCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHH
LYDYL
HHCCC
>Mature Secondary Structure
MKKCSMVIAGQPITYERFFVTVNRIATHFQNKVSSGSRIAFMLPNSLEIIAIYLACFQSG
CCCCEEEEECCCCEEHHHEEEHHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHHHHCC
CIAMPVNRRYAPPELEKVLQDAQPIYLIIEASKLFLLEKINWSATGIKKIFVLGEDRSHP
CEEECCCCCCCCHHHHHHHHCCCCEEEEEECCEEEEEEECCCCCCCEEEEEEEECCCCCC
YFSFDDLLTEPEKLPKQSISYETPAVIFYTSGSTGQPKGVVHTLSSIAAMLDSTSLTLEN
CCCHHHHHCCHHHCCHHHCCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEEE
ITADDKMIICEPQCHVSGFIETFSTLSREGTALVHDGFDMDWYLATVRHEKPTLAVHHID
CCCCCCEEEECCCHHHHHHHHHHHHHCCCCCEEEECCCCCEEEEEEEECCCCEEEEEHHH
TYIKLLDSGRCNTKTFQSFRGIYTGGDALPVAVQDKFLACAGKPIQVGYGMTEAIWLTVC
HHHHHHCCCCCCHHHHHHHCCEEECCCEEEEEECCCEEEECCCEEEECCCCCEEEEEEEE
RTPDLTQKSCIGKPVNGVTLRLVNAAGEDVRCGETGEILVKGDMVMHSYWNNPEETRKAF
CCCCCCHHHHCCCCCCCEEEEEEECCCCCEECCCCCCEEEECCEEEEECCCCHHHHHHHH
IKGWFKTGDCGIQDEQGYFYYKGRIKNIIIRNTSNIMPGEVEAAIYKHPAISAAAVIGVP
HHHCCCCCCCCCCCCCCEEEECCEEEEEEEECCCCCCCCCEEEEEECCCCCCEEEEEECC
DEAEGEVPIAFVVVKKSNQLTKEELYSFLIEQIAQYKIPEKIYFIDEMPLTNSGKINHKK
CCCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHH
LYDYL
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8969504; 9384377 [H]