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The map label for this gene is oprM [H]

Identifier: 54293646

GI number: 54293646

Start: 785175

End: 786644

Strand: Direct

Name: oprM [H]

Synonym: lpl0699

Alternate gene names: 54293646

Gene position: 785175-786644 (Clockwise)

Preceding gene: 54293645

Following gene: 54293653

Centisome position: 23.47

GC content: 41.63

Gene sequence:

>1470_bases
ATGGACAGAAAATTAGCGCTACTCTTCAGTATTTCTTGTTTATTTGGCTGTACTGTCGGTCCTGATTATCAACGCCCAGA
CATTAAAATTCAGGAGAAATGGCCAACAAGCAAAGTAGAGAAAAAAATTTCCAGTAGTGCCCCTGTCCATTTAACCTGGT
GGAAGGCATTTCATGATCCATTGCTTGATCGATACATTAGCGAGGCAGCTACTAATAACCTTGATATCAAAGCAGCAGAA
GCTCGTATCCGCCAAGCCCGCGCAATGAAACAAATTTCCCTTGCCAATTTTTATCCGCAGATCAATGGATCAGCAAGTGC
TAATTCCTTGCAAATCAGTAAGGGTACGTTGGAGGGTATTTTTGCAAATTCTCCAACATATACTCTAATTGATAGAAATC
AATGGATATATAATTTGGGTTTTGATGCACAATGGGAGTTGGATTTATTTGGTAAAACAAGAAGAAAAGTTGAAGCAAGC
AAAGCAAGCCTGGAAGAGGCTTATGAATCACGCCGTACCATTTTACTATCTACAATAGCCGAGATCGCAAGAAGTTATAT
AGAATTGAGAGGCGCACAACAGTTGTATGGTATTGCGCAAAAAAATGTGGAATTGCAAAGAAAAACGTTGGCTCTTGTCC
GCCAACGCTATAAAGCAGGTGTTAGCAGCGAAGTCGATGTTTCTTTGGCCGAAGGCCAGTTAAAAAATACAGAATCTGTT
TTACCCAATTATATTGCTCAAATACAGGTAAATGCTTATCAAATTGCCGTTATGCTGGGTAAAGATCCGCAAACAATTCT
CAAGGATATTGCAAAAATTCAATCTTTACCCAAGCCACCGCAAAAAGTACCTATGGGATTACGTTCAGATATTCTGCGGC
AGCGTCCAGACATAAGGGCAACAGAGAGGCAATTGGCCTCTGCCGTAGCTGAAATTGGTGTGGCAGTTGCTGACTTTTTC
CCGCAATTTAATCTTTCTGTTTCCAATCTTGCTTTTCAGAGCACTTCTGTTGATACGTTATTTCAATCCAGCAGTGGATT
TTGGCTCATGGGTCCTCAAGTCAGTTTACCCATATTTCATGGTGGCCAGATTAGAGCTAATGTTGAGAGTAAGAAGGCGG
TTGTTCAGCAAGCAGCAATACAATACAAAAAAATAATCCTCCAGGCATTGCAAGAGGCTCAAAGTGCTCTCGTCCGCTAT
AGCCAATCGCTCGATACAAACCATAGCTTGCGCGCTGCCATTATTGCTAACAAGAGAGCTGTGCAATTTGCCAAGAAACG
ATTTCAATACGGTGAGGATGATTATTTATCACTACTGCAATCAGAGGCTGCTCTTCTGAATGCCGAGACTCAAGAAGTTA
ATTCGGAGGTTTCTTCATTGACTGCGTTGATTAGTCTATATAAAGCGCTTGGCGGAGGTTGGGAAAACTTTGAGGCTTCA
AGTAGCCCTAAACATCCGCGCATACTCTAA

Upstream 100 bases:

>100_bases
TAGAAGTTTTTATCACAACATGGCCACTTCTATTGGTAGGGGCTGTTACATTATTTATTGCTGGCTGGTTTTTTAAGCAG
CGTATGGAGTAAGAAACAGT

Downstream 100 bases:

>100_bases
AATCAGGAGTTTTATCAATAAATTAATGTAATAAGTTATGGATAAGGTAGGTCATTATCTTGAATCGTTCGGGCTGAGGC
GGCATTTATGCCGTCTCGAA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 489; Mature: 489

Protein sequence:

>489_residues
MDRKLALLFSISCLFGCTVGPDYQRPDIKIQEKWPTSKVEKKISSSAPVHLTWWKAFHDPLLDRYISEAATNNLDIKAAE
ARIRQARAMKQISLANFYPQINGSASANSLQISKGTLEGIFANSPTYTLIDRNQWIYNLGFDAQWELDLFGKTRRKVEAS
KASLEEAYESRRTILLSTIAEIARSYIELRGAQQLYGIAQKNVELQRKTLALVRQRYKAGVSSEVDVSLAEGQLKNTESV
LPNYIAQIQVNAYQIAVMLGKDPQTILKDIAKIQSLPKPPQKVPMGLRSDILRQRPDIRATERQLASAVAEIGVAVADFF
PQFNLSVSNLAFQSTSVDTLFQSSSGFWLMGPQVSLPIFHGGQIRANVESKKAVVQQAAIQYKKIILQALQEAQSALVRY
SQSLDTNHSLRAAIIANKRAVQFAKKRFQYGEDDYLSLLQSEAALLNAETQEVNSEVSSLTALISLYKALGGGWENFEAS
SSPKHPRIL

Sequences:

>Translated_489_residues
MDRKLALLFSISCLFGCTVGPDYQRPDIKIQEKWPTSKVEKKISSSAPVHLTWWKAFHDPLLDRYISEAATNNLDIKAAE
ARIRQARAMKQISLANFYPQINGSASANSLQISKGTLEGIFANSPTYTLIDRNQWIYNLGFDAQWELDLFGKTRRKVEAS
KASLEEAYESRRTILLSTIAEIARSYIELRGAQQLYGIAQKNVELQRKTLALVRQRYKAGVSSEVDVSLAEGQLKNTESV
LPNYIAQIQVNAYQIAVMLGKDPQTILKDIAKIQSLPKPPQKVPMGLRSDILRQRPDIRATERQLASAVAEIGVAVADFF
PQFNLSVSNLAFQSTSVDTLFQSSSGFWLMGPQVSLPIFHGGQIRANVESKKAVVQQAAIQYKKIILQALQEAQSALVRY
SQSLDTNHSLRAAIIANKRAVQFAKKRFQYGEDDYLSLLQSEAALLNAETQEVNSEVSSLTALISLYKALGGGWENFEAS
SSPKHPRIL
>Mature_489_residues
MDRKLALLFSISCLFGCTVGPDYQRPDIKIQEKWPTSKVEKKISSSAPVHLTWWKAFHDPLLDRYISEAATNNLDIKAAE
ARIRQARAMKQISLANFYPQINGSASANSLQISKGTLEGIFANSPTYTLIDRNQWIYNLGFDAQWELDLFGKTRRKVEAS
KASLEEAYESRRTILLSTIAEIARSYIELRGAQQLYGIAQKNVELQRKTLALVRQRYKAGVSSEVDVSLAEGQLKNTESV
LPNYIAQIQVNAYQIAVMLGKDPQTILKDIAKIQSLPKPPQKVPMGLRSDILRQRPDIRATERQLASAVAEIGVAVADFF
PQFNLSVSNLAFQSTSVDTLFQSSSGFWLMGPQVSLPIFHGGQIRANVESKKAVVQQAAIQYKKIILQALQEAQSALVRY
SQSLDTNHSLRAAIIANKRAVQFAKKRFQYGEDDYLSLLQSEAALLNAETQEVNSEVSSLTALISLYKALGGGWENFEAS
SSPKHPRIL

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=465, Percent_Identity=29.6774193548387, Blast_Score=189, Evalue=5e-49,
Organism=Escherichia coli, GI2367348, Length=476, Percent_Identity=21.8487394957983, Blast_Score=100, Evalue=3e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 54485; Mature: 54485

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRKLALLFSISCLFGCTVGPDYQRPDIKIQEKWPTSKVEKKISSSAPVHLTWWKAFHDP
CCCCEEHHHHHHHHHCCCCCCCCCCCCCEEECCCCHHHHHHHHCCCCCEEEEHHHHHHHH
LLDRYISEAATNNLDIKAAEARIRQARAMKQISLANFYPQINGSASANSLQISKGTLEGI
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCEE
FANSPTYTLIDRNQWIYNLGFDAQWELDLFGKTRRKVEASKASLEEAYESRRTILLSTIA
ECCCCEEEEEECCCEEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIARSYIELRGAQQLYGIAQKNVELQRKTLALVRQRYKAGVSSEVDVSLAEGQLKNTESV
HHHHHHHHHHCHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHH
LPNYIAQIQVNAYQIAVMLGKDPQTILKDIAKIQSLPKPPQKVPMGLRSDILRQRPDIRA
HHHHHHHEEEEEEEEEEEECCCHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHCCCCCHH
TERQLASAVAEIGVAVADFFPQFNLSVSNLAFQSTSVDTLFQSSSGFWLMGPQVSLPIFH
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEECCCCHHHHHCCCCCEEEECCCEECEEEE
GGQIRANVESKKAVVQQAAIQYKKIILQALQEAQSALVRYSQSLDTNHSLRAAIIANKRA
CCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHH
VQFAKKRFQYGEDDYLSLLQSEAALLNAETQEVNSEVSSLTALISLYKALGGGWENFEAS
HHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
SSPKHPRIL
CCCCCCCCC
>Mature Secondary Structure
MDRKLALLFSISCLFGCTVGPDYQRPDIKIQEKWPTSKVEKKISSSAPVHLTWWKAFHDP
CCCCEEHHHHHHHHHCCCCCCCCCCCCCEEECCCCHHHHHHHHCCCCCEEEEHHHHHHHH
LLDRYISEAATNNLDIKAAEARIRQARAMKQISLANFYPQINGSASANSLQISKGTLEGI
HHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEECCCCCEE
FANSPTYTLIDRNQWIYNLGFDAQWELDLFGKTRRKVEASKASLEEAYESRRTILLSTIA
ECCCCEEEEEECCCEEEECCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIARSYIELRGAQQLYGIAQKNVELQRKTLALVRQRYKAGVSSEVDVSLAEGQLKNTESV
HHHHHHHHHHCHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCHHHH
LPNYIAQIQVNAYQIAVMLGKDPQTILKDIAKIQSLPKPPQKVPMGLRSDILRQRPDIRA
HHHHHHHEEEEEEEEEEEECCCHHHHHHHHHHHHCCCCCHHHCCCCHHHHHHHCCCCCHH
TERQLASAVAEIGVAVADFFPQFNLSVSNLAFQSTSVDTLFQSSSGFWLMGPQVSLPIFH
HHHHHHHHHHHHHHHHHHHCCCCCCCHHHHEECCCCHHHHHCCCCCEEEECCCEECEEEE
GGQIRANVESKKAVVQQAAIQYKKIILQALQEAQSALVRYSQSLDTNHSLRAAIIANKRA
CCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEHHHHHH
VQFAKKRFQYGEDDYLSLLQSEAALLNAETQEVNSEVSSLTALISLYKALGGGWENFEAS
HHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCC
SSPKHPRIL
CCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]