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The map label for this gene is estA [H]

Identifier: 54293361

GI number: 54293361

Start: 458284

End: 458943

Strand: Direct

Name: estA [H]

Synonym: lpl0410

Alternate gene names: 54293361

Gene position: 458284-458943 (Clockwise)

Preceding gene: 54293355

Following gene: 54293362

Centisome position: 13.7

GC content: 40.76

Gene sequence:

>660_bases
TTGAGCGTTTATATGAAGGAACCTTTAGAGAAAGCACAAGCTTGTGTCATATGGATGCATGGGTTGGGAGCTGATGCCTC
TGATATGATGGGTTTAGCTGATCAGCTAACAATAGAAGATACCGCTTTAAGGCATGTATTTCTTGATGCTCCTCGAAGAC
CAGTGACTCTGAATGGAGGAATGGTTATGCCTGCGTGGTATGATATTTATGGATTAGGTTTTGTCGATGAAGAAGATAGG
TTTGGCATTGAGCAATCTGAGTTGCTAATACGCAAGGTGGTTGACGCTCAATATAATTGTGGCTTTAAACCTCATCAAAT
TTTTTTAGCTGGCTTTTCTCAAGGGGGAGCTATGGCATTGCACACGGCTCTTAATATGACTGAGCGATTATGCGGCGTTA
TTGCATTGTCCGCTTATTTGCCCTTGGCTAAGCATAATAAGCCGCAACTCGATAAGAATACTCCCATCTTTATGGGAGCA
GGACAATTTGATCCTTTAGTTCTGCCAAAATGGACTCTACAGAGTAAGGATTGGCTCTTGGCTAATGGGTATAATGAAGT
CTCTTTTCATCAATACCCTATGGAACATTCCATATGTTTTGAAGAAATTAAAGATCTTAGCCTTTGGCTTAATAAGCAGG
TTCAAGGAGTTTTACAATGA

Upstream 100 bases:

>100_bases
GTCAATATGCTTATAAATTGTTCAATATATTTTTAAATGTTAAACTTGCACTGCTCTATTGCATTGGCGATAATTTACGT
TTTTGCGTGGAGAATTTGTT

Downstream 100 bases:

>100_bases
CAATAGTAAAAAGGTCAAGAACAGTACCTTATTCTTGTGAGCAAATGTATGGACTGGTTAATGATGTTGAACATTATTCC
GAGTTTCTACCTTATTGTGC

Product: hypothetical protein

Products: NA

Alternate protein names: Esterase I [H]

Number of amino acids: Translated: 219; Mature: 218

Protein sequence:

>219_residues
MSVYMKEPLEKAQACVIWMHGLGADASDMMGLADQLTIEDTALRHVFLDAPRRPVTLNGGMVMPAWYDIYGLGFVDEEDR
FGIEQSELLIRKVVDAQYNCGFKPHQIFLAGFSQGGAMALHTALNMTERLCGVIALSAYLPLAKHNKPQLDKNTPIFMGA
GQFDPLVLPKWTLQSKDWLLANGYNEVSFHQYPMEHSICFEEIKDLSLWLNKQVQGVLQ

Sequences:

>Translated_219_residues
MSVYMKEPLEKAQACVIWMHGLGADASDMMGLADQLTIEDTALRHVFLDAPRRPVTLNGGMVMPAWYDIYGLGFVDEEDR
FGIEQSELLIRKVVDAQYNCGFKPHQIFLAGFSQGGAMALHTALNMTERLCGVIALSAYLPLAKHNKPQLDKNTPIFMGA
GQFDPLVLPKWTLQSKDWLLANGYNEVSFHQYPMEHSICFEEIKDLSLWLNKQVQGVLQ
>Mature_218_residues
SVYMKEPLEKAQACVIWMHGLGADASDMMGLADQLTIEDTALRHVFLDAPRRPVTLNGGMVMPAWYDIYGLGFVDEEDRF
GIEQSELLIRKVVDAQYNCGFKPHQIFLAGFSQGGAMALHTALNMTERLCGVIALSAYLPLAKHNKPQLDKNTPIFMGAG
QFDPLVLPKWTLQSKDWLLANGYNEVSFHQYPMEHSICFEEIKDLSLWLNKQVQGVLQ

Specific function: Hydrolyzes carboxylic ester bonds with relatively broad substrate specificity [H]

COG id: COG0400

COG function: function code R; Predicted esterase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AB hydrolase 2 family [H]

Homologues:

Organism=Homo sapiens, GI5453722, Length=212, Percent_Identity=37.2641509433962, Blast_Score=119, Evalue=3e-27,
Organism=Homo sapiens, GI9966764, Length=215, Percent_Identity=35.3488372093023, Blast_Score=111, Evalue=5e-25,
Organism=Homo sapiens, GI20270341, Length=221, Percent_Identity=26.2443438914027, Blast_Score=82, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17508057, Length=215, Percent_Identity=32.093023255814, Blast_Score=111, Evalue=2e-25,
Organism=Saccharomyces cerevisiae, GI6323147, Length=217, Percent_Identity=34.1013824884793, Blast_Score=105, Evalue=4e-24,
Organism=Drosophila melanogaster, GI45553055, Length=214, Percent_Identity=35.0467289719626, Blast_Score=108, Evalue=3e-24,
Organism=Drosophila melanogaster, GI45553057, Length=214, Percent_Identity=35.0467289719626, Blast_Score=108, Evalue=3e-24,
Organism=Drosophila melanogaster, GI21357257, Length=214, Percent_Identity=35.0467289719626, Blast_Score=108, Evalue=3e-24,
Organism=Drosophila melanogaster, GI161078179, Length=215, Percent_Identity=26.046511627907, Blast_Score=69, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003140 [H]

Pfam domain/function: PF02230 Abhydrolase_2 [H]

EC number: =3.1.1.1 [H]

Molecular weight: Translated: 24635; Mature: 24504

Theoretical pI: Translated: 5.25; Mature: 5.25

Prosite motif: PS00659 GLYCOSYL_HYDROL_F5

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
5.0 %Met     (Translated Protein)
6.8 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
4.6 %Met     (Mature Protein)
6.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVYMKEPLEKAQACVIWMHGLGADASDMMGLADQLTIEDTALRHVFLDAPRRPVTLNGG
CCCCHHHHHHHHHEEEEEEECCCCCHHHHHCCHHHEEEHHHHHHHHHHCCCCCCEEECCC
MVMPAWYDIYGLGFVDEEDRFGIEQSELLIRKVVDAQYNCGFKPHQIFLAGFSQGGAMAL
EECCCHHHHEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHH
HTALNMTERLCGVIALSAYLPLAKHNKPQLDKNTPIFMGAGQFDPLVLPKWTLQSKDWLL
HHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCEEEECCCCCCEECCCCCCCCCCEEE
ANGYNEVSFHQYPMEHSICFEEIKDLSLWLNKQVQGVLQ
ECCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure 
SVYMKEPLEKAQACVIWMHGLGADASDMMGLADQLTIEDTALRHVFLDAPRRPVTLNGG
CCCHHHHHHHHHEEEEEEECCCCCHHHHHCCHHHEEEHHHHHHHHHHCCCCCCEEECCC
MVMPAWYDIYGLGFVDEEDRFGIEQSELLIRKVVDAQYNCGFKPHQIFLAGFSQGGAMAL
EECCCHHHHEECCCCCCCCCCCCCHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCHHHH
HTALNMTERLCGVIALSAYLPLAKHNKPQLDKNTPIFMGAGQFDPLVLPKWTLQSKDWLL
HHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCCCCCEEEECCCCCCEECCCCCCCCCCEEE
ANGYNEVSFHQYPMEHSICFEEIKDLSLWLNKQVQGVLQ
ECCCCCCCEEECCCHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA