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The map label for this gene is tufA [H]

Identifier: 54293319

GI number: 54293319

Start: 430673

End: 431863

Strand: Direct

Name: tufA [H]

Synonym: lpl0367

Alternate gene names: 54293319

Gene position: 430673-431863 (Clockwise)

Preceding gene: 54293318

Following gene: 54293320

Centisome position: 12.87

GC content: 44.0

Gene sequence:

>1191_bases
ATGGCGAAGGAAAAATTTGAACGTAAGAAGCCGCACGTAAACGTGGGCACGATTGGTCACGTAGACCATGGTAAGACGAC
ATTGACAGCAGCTATTACAACGATTATGGCGAAGAAATATGGTGGTACAGCGAAGGCGTACGATCAAATTGATGCTGCGC
CAGAAGAAAGAGAGCGTGGTATTACTATATCTACAGCCCATGTTGAATACGAATCAGCGAGCAGACATTATGCACACGTA
GACTGCCCGGGACATGCTGACTATGTTAAGAACATGATTACTGGTGCTGCGCAAATGGACGGAGCGATACTGGTTGTATC
AGCAGCTGATGGTCCTATGCCACAAACGAGGGAACACATTCTATTGTCTCGCCAGGTAGGTGTTCCATATATTGTTGTGT
TCATGAACAAAGCGGATATGGTTGATGACCCTGAGTTATTAGAGTTAGTGGAAATGGAAGTGCGAGATTTATTAAGCAGT
TACGATTTCCCAGGGGATGATATACCTATTATTGTTGGTTCAGCTTTGAAAGCATTGGAAGGTGAAGACAGTGATATAGG
TGTTAAGGCTATTGAGAAATTAGTTGAAACAATGGATTCATACATTCCTGAGCCAGTTAGAAACATAGACAAGCCATTCT
TGTTGCCGATTGAAGACGTGTTTTCAATTTCTGGACGCGGAACAGTGGTAACTGGTCGTGTTGAGAGTGGAATTGTTAAA
GTTGGTGAGGAAGTTGAAATTGTTGGAATAAGAGACACCCAAAAGACGACTTGTACTGGTGTTGAGATGTTCCGTAAATT
ACTTGATGAAGGTCGAGCCGGTGATAACGTTGGTGTGTTATTACGCGGTACGAAGCGAGATGAAGTGGAGCGTGGACAGG
TATTGGCTAAGCCAGGAACCATCAAGCCACACACCAAGTTTGAAGCAGAAGTGTATGTATTATCGAAGGAAGAAGGCGGA
CGTCACACTCCATTCTTTAATGGATACCGTCCACAATTCTATTTCAGAACCACTGACGTGACAGGTACTTGTGACTTGCC
ATCAGGAGTTGAAATGGTAATGCCTGGAGATAATGTGCAATTAGTTGTTAGCTTGCATGCTCCGATTGCGATGGATGAAG
GTTTAAGATTCGCAATTAGAGAGGGTGGCCGAACTGTTGGTGCCGGTGTAGTCGCTAAAATAATCGAGTAA

Upstream 100 bases:

>100_bases
GAGCAACCTACACTATGGAGTTTTGTAAGTACGCAGAAGCACCAACAAATATTGCTGAAGCAATTATCAAGAAACAATAA
AAGTTAACGAGGTTATTGAA

Downstream 100 bases:

>100_bases
AAAAAATGAGCAGCAATCAAAATATTAAAATAAGATTAAAATCCTTTGATCATCGGTTGATTGATCTATCAACACGAGAA
ATCGTTGATACTGCGAAAAG

Product: elongation factor Tu

Products: NA

Alternate protein names: EF-Tu [H]

Number of amino acids: Translated: 396; Mature: 395

Protein sequence:

>396_residues
MAKEKFERKKPHVNVGTIGHVDHGKTTLTAAITTIMAKKYGGTAKAYDQIDAAPEERERGITISTAHVEYESASRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLSRQVGVPYIVVFMNKADMVDDPELLELVEMEVRDLLSS
YDFPGDDIPIIVGSALKALEGEDSDIGVKAIEKLVETMDSYIPEPVRNIDKPFLLPIEDVFSISGRGTVVTGRVESGIVK
VGEEVEIVGIRDTQKTTCTGVEMFRKLLDEGRAGDNVGVLLRGTKRDEVERGQVLAKPGTIKPHTKFEAEVYVLSKEEGG
RHTPFFNGYRPQFYFRTTDVTGTCDLPSGVEMVMPGDNVQLVVSLHAPIAMDEGLRFAIREGGRTVGAGVVAKIIE

Sequences:

>Translated_396_residues
MAKEKFERKKPHVNVGTIGHVDHGKTTLTAAITTIMAKKYGGTAKAYDQIDAAPEERERGITISTAHVEYESASRHYAHV
DCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLSRQVGVPYIVVFMNKADMVDDPELLELVEMEVRDLLSS
YDFPGDDIPIIVGSALKALEGEDSDIGVKAIEKLVETMDSYIPEPVRNIDKPFLLPIEDVFSISGRGTVVTGRVESGIVK
VGEEVEIVGIRDTQKTTCTGVEMFRKLLDEGRAGDNVGVLLRGTKRDEVERGQVLAKPGTIKPHTKFEAEVYVLSKEEGG
RHTPFFNGYRPQFYFRTTDVTGTCDLPSGVEMVMPGDNVQLVVSLHAPIAMDEGLRFAIREGGRTVGAGVVAKIIE
>Mature_395_residues
AKEKFERKKPHVNVGTIGHVDHGKTTLTAAITTIMAKKYGGTAKAYDQIDAAPEERERGITISTAHVEYESASRHYAHVD
CPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHILLSRQVGVPYIVVFMNKADMVDDPELLELVEMEVRDLLSSY
DFPGDDIPIIVGSALKALEGEDSDIGVKAIEKLVETMDSYIPEPVRNIDKPFLLPIEDVFSISGRGTVVTGRVESGIVKV
GEEVEIVGIRDTQKTTCTGVEMFRKLLDEGRAGDNVGVLLRGTKRDEVERGQVLAKPGTIKPHTKFEAEVYVLSKEEGGR
HTPFFNGYRPQFYFRTTDVTGTCDLPSGVEMVMPGDNVQLVVSLHAPIAMDEGLRFAIREGGRTVGAGVVAKIIE

Specific function: This protein promotes the GTP-dependent binding of aminoacyl-tRNA to the A-site of ribosomes during protein biosynthesis [H]

COG id: COG0050

COG function: function code J; GTPases - translation elongation factors

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding elongation factor family. EF-Tu/EF-1A subfamily [H]

Homologues:

Organism=Homo sapiens, GI34147630, Length=394, Percent_Identity=56.8527918781726, Blast_Score=488, Evalue=1e-138,
Organism=Homo sapiens, GI223555963, Length=444, Percent_Identity=30.1801801801802, Blast_Score=170, Evalue=2e-42,
Organism=Homo sapiens, GI5729864, Length=444, Percent_Identity=30.1801801801802, Blast_Score=170, Evalue=3e-42,
Organism=Homo sapiens, GI4503475, Length=455, Percent_Identity=29.6703296703297, Blast_Score=158, Evalue=7e-39,
Organism=Homo sapiens, GI4503471, Length=448, Percent_Identity=28.7946428571429, Blast_Score=156, Evalue=4e-38,
Organism=Homo sapiens, GI194018520, Length=338, Percent_Identity=30.7692307692308, Blast_Score=128, Evalue=9e-30,
Organism=Homo sapiens, GI194097354, Length=338, Percent_Identity=30.7692307692308, Blast_Score=128, Evalue=9e-30,
Organism=Homo sapiens, GI194018522, Length=338, Percent_Identity=30.7692307692308, Blast_Score=128, Evalue=9e-30,
Organism=Homo sapiens, GI46094014, Length=328, Percent_Identity=31.7073170731707, Blast_Score=126, Evalue=4e-29,
Organism=Homo sapiens, GI54607086, Length=348, Percent_Identity=27.5862068965517, Blast_Score=120, Evalue=2e-27,
Organism=Homo sapiens, GI4503507, Length=277, Percent_Identity=26.7148014440433, Blast_Score=84, Evalue=3e-16,
Organism=Homo sapiens, GI157426893, Length=253, Percent_Identity=25.296442687747, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1790412, Length=395, Percent_Identity=82.5316455696203, Blast_Score=647, Evalue=0.0,
Organism=Escherichia coli, GI1789737, Length=395, Percent_Identity=82.5316455696203, Blast_Score=646, Evalue=0.0,
Organism=Escherichia coli, GI2367247, Length=380, Percent_Identity=29.2105263157895, Blast_Score=132, Evalue=3e-32,
Organism=Escherichia coli, GI48994988, Length=239, Percent_Identity=32.6359832635983, Blast_Score=101, Evalue=7e-23,
Organism=Escherichia coli, GI1789108, Length=428, Percent_Identity=26.4018691588785, Blast_Score=94, Evalue=2e-20,
Organism=Escherichia coli, GI1790835, Length=146, Percent_Identity=32.1917808219178, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI1788922, Length=330, Percent_Identity=25.1515151515152, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17556456, Length=399, Percent_Identity=51.6290726817043, Blast_Score=397, Evalue=1e-111,
Organism=Caenorhabditis elegans, GI25141371, Length=387, Percent_Identity=45.7364341085271, Blast_Score=348, Evalue=2e-96,
Organism=Caenorhabditis elegans, GI17552884, Length=454, Percent_Identity=30.8370044052863, Blast_Score=162, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17569207, Length=454, Percent_Identity=30.8370044052863, Blast_Score=162, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI32566303, Length=435, Percent_Identity=29.6551724137931, Blast_Score=138, Evalue=5e-33,
Organism=Caenorhabditis elegans, GI115532067, Length=438, Percent_Identity=28.5388127853881, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI32566629, Length=440, Percent_Identity=27.0454545454545, Blast_Score=130, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI115532065, Length=438, Percent_Identity=28.5388127853881, Blast_Score=129, Evalue=3e-30,
Organism=Caenorhabditis elegans, GI17506081, Length=300, Percent_Identity=28, Blast_Score=112, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI32566301, Length=147, Percent_Identity=39.4557823129252, Blast_Score=91, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17509919, Length=457, Percent_Identity=24.945295404814, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17557151, Length=238, Percent_Identity=28.1512605042017, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6324761, Length=396, Percent_Identity=66.1616161616162, Blast_Score=533, Evalue=1e-152,
Organism=Saccharomyces cerevisiae, GI6325337, Length=441, Percent_Identity=30.8390022675737, Blast_Score=160, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6319594, Length=441, Percent_Identity=30.8390022675737, Blast_Score=160, Evalue=3e-40,
Organism=Saccharomyces cerevisiae, GI6322937, Length=353, Percent_Identity=25.7790368271955, Blast_Score=98, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6320377, Length=349, Percent_Identity=28.9398280802292, Blast_Score=96, Evalue=9e-21,
Organism=Saccharomyces cerevisiae, GI6320863, Length=287, Percent_Identity=25.0871080139373, Blast_Score=80, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6323320, Length=240, Percent_Identity=28.3333333333333, Blast_Score=74, Evalue=4e-14,
Organism=Saccharomyces cerevisiae, GI6323098, Length=171, Percent_Identity=30.9941520467836, Blast_Score=67, Evalue=7e-12,
Organism=Drosophila melanogaster, GI281363316, Length=393, Percent_Identity=56.7430025445293, Blast_Score=476, Evalue=1e-135,
Organism=Drosophila melanogaster, GI17864358, Length=393, Percent_Identity=56.7430025445293, Blast_Score=476, Evalue=1e-135,
Organism=Drosophila melanogaster, GI19921738, Length=386, Percent_Identity=51.0362694300518, Blast_Score=404, Evalue=1e-113,
Organism=Drosophila melanogaster, GI45553807, Length=455, Percent_Identity=29.6703296703297, Blast_Score=167, Evalue=1e-41,
Organism=Drosophila melanogaster, GI45553816, Length=455, Percent_Identity=29.6703296703297, Blast_Score=167, Evalue=1e-41,
Organism=Drosophila melanogaster, GI24651721, Length=455, Percent_Identity=29.6703296703297, Blast_Score=167, Evalue=1e-41,
Organism=Drosophila melanogaster, GI17864154, Length=455, Percent_Identity=29.6703296703297, Blast_Score=167, Evalue=1e-41,
Organism=Drosophila melanogaster, GI24652838, Length=449, Percent_Identity=30.2895322939866, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI17137572, Length=449, Percent_Identity=30.2895322939866, Blast_Score=159, Evalue=3e-39,
Organism=Drosophila melanogaster, GI19922690, Length=305, Percent_Identity=30.4918032786885, Blast_Score=131, Evalue=8e-31,
Organism=Drosophila melanogaster, GI45550900, Length=447, Percent_Identity=27.5167785234899, Blast_Score=129, Evalue=4e-30,
Organism=Drosophila melanogaster, GI17137380, Length=355, Percent_Identity=30.1408450704225, Blast_Score=126, Evalue=2e-29,
Organism=Drosophila melanogaster, GI24647046, Length=272, Percent_Identity=27.2058823529412, Blast_Score=90, Evalue=3e-18,
Organism=Drosophila melanogaster, GI24647048, Length=272, Percent_Identity=27.2058823529412, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI28572034, Length=179, Percent_Identity=33.5195530726257, Blast_Score=69, Evalue=8e-12,

Paralogues:

None

Copy number: 20568 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000795
- InterPro:   IPR005225
- InterPro:   IPR009001
- InterPro:   IPR004161
- InterPro:   IPR004541
- InterPro:   IPR004160
- InterPro:   IPR009000 [H]

Pfam domain/function: PF00009 GTP_EFTU; PF03144 GTP_EFTU_D2; PF03143 GTP_EFTU_D3 [H]

EC number: NA

Molecular weight: Translated: 43194; Mature: 43063

Theoretical pI: Translated: 5.04; Mature: 5.04

Prosite motif: PS00301 EFACTOR_GTP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKEKFERKKPHVNVGTIGHVDHGKTTLTAAITTIMAKKYGGTAKAYDQIDAAPEERERG
CCCHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHCC
ITISTAHVEYESASRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
CEEEEEEEEECCCCCCEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLSRQVGVPYIVVFMNKADMVDDPELLELVEMEVRDLLSSYDFPGDDIPIIVGSALKALE
EEECCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCHHHHHC
GEDSDIGVKAIEKLVETMDSYIPEPVRNIDKPFLLPIEDVFSISGRGTVVTGRVESGIVK
CCCCCCCHHHHHHHHHHHHHHCCHHHHHCCCCEEEEHHHHHCCCCCCEEEECCCCCCCEE
VGEEVEIVGIRDTQKTTCTGVEMFRKLLDEGRAGDNVGVLLRGTKRDEVERGQVLAKPGT
CCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCEEECCCC
IKPHTKFEAEVYVLSKEEGGRHTPFFNGYRPQFYFRTTDVTGTCDLPSGVEMVMPGDNVQ
CCCCCCCEEEEEEEEECCCCCCCCCCCCCCCEEEEEECCCEEECCCCCCCEEEECCCCEE
LVVSLHAPIAMDEGLRFAIREGGRTVGAGVVAKIIE
EEEEECCCEECCCCCEEEECCCCCEEHHHHHHHHCC
>Mature Secondary Structure 
AKEKFERKKPHVNVGTIGHVDHGKTTLTAAITTIMAKKYGGTAKAYDQIDAAPEERERG
CCHHHHHCCCCCCCCEEECCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHCCCCHHHHCC
ITISTAHVEYESASRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSAADGPMPQTREHI
CEEEEEEEEECCCCCCEEEEECCCHHHHHHHHHHCHHHCCCEEEEEECCCCCCCCCHHHE
LLSRQVGVPYIVVFMNKADMVDDPELLELVEMEVRDLLSSYDFPGDDIPIIVGSALKALE
EEECCCCCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCHHHHHC
GEDSDIGVKAIEKLVETMDSYIPEPVRNIDKPFLLPIEDVFSISGRGTVVTGRVESGIVK
CCCCCCCHHHHHHHHHHHHHHCCHHHHHCCCCEEEEHHHHHCCCCCCEEEECCCCCCCEE
VGEEVEIVGIRDTQKTTCTGVEMFRKLLDEGRAGDNVGVLLRGTKRDEVERGQVLAKPGT
CCCEEEEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCEEECCCC
IKPHTKFEAEVYVLSKEEGGRHTPFFNGYRPQFYFRTTDVTGTCDLPSGVEMVMPGDNVQ
CCCCCCCEEEEEEEEECCCCCCCCCCCCCCCEEEEEECCCEEECCCCCCCEEEECCCCEE
LVVSLHAPIAMDEGLRFAIREGGRTVGAGVVAKIIE
EEEEECCCEECCCCCEEEECCCCCEEHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA