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The map label for this gene is asnS

Identifier: 54020547

GI number: 54020547

Start: 623664

End: 624719

Strand: Reverse

Name: asnS

Synonym: mhp497

Alternate gene names: 54020547

Gene position: 624719-623664 (Counterclockwise)

Preceding gene: 54020548

Following gene: 54020546

Centisome position: 69.98

GC content: 25.85

Gene sequence:

>1056_bases
TTGTTAGAATTTCCTTACTACGCAAAAAAATATAAAAAAGGAAACTCAATAACTGTGAATTTTAAGAATTTTAAGCATGA
TTTTATAAAGCACATATCAAGTGAAAGTTATGCCAAAATATTAACAATTTATTCAGAAATTAATTTTTTAACATACAAGT
GAATGCGCGATAACAATGTAAAAATAGTAAATTGTCCAATAACAACACAAAGTATTTCAAGTCCTATGGGGCTCGGAAGT
GATTCACTACCTTATAAAGTTATTTCGAAAAATAATCCGAATTTTGAGTTTTATTTAGCAGACAGCATGCAATTTCATTT
GGAATTATTTTTAAGAACAAAAAATGTTGAATCAGTCGGATATATTGCCCCAACATTTCGTGGCGAAAATGTTGATGAAA
GACATTTACATCAATTTAATCATTTTGAGATTGAAACAAAAAAACCATTAGTTGAAACAAAAAAAATGATTATAAATTAT
CTAAAATATTTAGTTAAAAATTTAGTTAAAACACATTCCGCTGAACTAAATTATTTTAATGAAAATAATGTACCAAGACT
TTTGAAGTGACTAAACTTTGATCAATATAATTTATTATTTACAAATGCTATAAAAATATTAGATGAAAAATTTAAAGAAG
GCATCGATCGAACCGAACTACTTTTAAATATAAATTCAAAAGGTGAAAAATTTTTAATTAATTATTTTTCTGATAAAATT
TTATGGTTAGATAATTTTCCAGAAATTAATGTACCTTTTTATCAAAAAAGTAAAGATGGTTTTGCGATTAATTCAGATTT
ATTAATGGGAATCGGAGAAATAGTTGGAAGTGGTGAAAGGTGTTTTGATTATAATGAAACATTAAAATCCCTTTTAAACC
ACAAAAATAACCCACAAAATTATAAATGATATTTAGAAATGAAAGAAAAAACACCGATGATTACTTCTGGATTTGGTTTA
GGAATGGAAAGATTGATGCTATTTTTAATTGGAAAAAATGATATACGCGAAGTTCAACTAATCCCGAGAGAAACTGACAA
GGAAATTCTCCCCTAA

Upstream 100 bases:

>100_bases
ATTTTTTTTAATTAAAAATTATTTTTTAATAATTAAGTTTGATACTAATTTATGAAAAAAATTTTAAAAATTTTGCTTTT
GTTTTAAAAAACTAAAAATT

Downstream 100 bases:

>100_bases
AATGACAGAAACGATCAAAATAATTGCTGCATATTTGCAGGAATTTAACAAAAAAGAAGGTCTAAATTTAAGAATTTTCC
TCAAAGGAAGTTATATTCAC

Product: asparagine synthetase A

Products: NA

Alternate protein names: Asparagine--tRNA ligase; AsnRS [H]

Number of amino acids: Translated: 351; Mature: 351

Protein sequence:

>351_residues
MLEFPYYAKKYKKGNSITVNFKNFKHDFIKHISSESYAKILTIYSEINFLTYKWMRDNNVKIVNCPITTQSISSPMGLGS
DSLPYKVISKNNPNFEFYLADSMQFHLELFLRTKNVESVGYIAPTFRGENVDERHLHQFNHFEIETKKPLVETKKMIINY
LKYLVKNLVKTHSAELNYFNENNVPRLLKWLNFDQYNLLFTNAIKILDEKFKEGIDRTELLLNINSKGEKFLINYFSDKI
LWLDNFPEINVPFYQKSKDGFAINSDLLMGIGEIVGSGERCFDYNETLKSLLNHKNNPQNYKWYLEMKEKTPMITSGFGL
GMERLMLFLIGKNDIREVQLIPRETDKEILP

Sequences:

>Translated_351_residues
MLEFPYYAKKYKKGNSITVNFKNFKHDFIKHISSESYAKILTIYSEINFLTYK*MRDNNVKIVNCPITTQSISSPMGLGS
DSLPYKVISKNNPNFEFYLADSMQFHLELFLRTKNVESVGYIAPTFRGENVDERHLHQFNHFEIETKKPLVETKKMIINY
LKYLVKNLVKTHSAELNYFNENNVPRLLK*LNFDQYNLLFTNAIKILDEKFKEGIDRTELLLNINSKGEKFLINYFSDKI
LWLDNFPEINVPFYQKSKDGFAINSDLLMGIGEIVGSGERCFDYNETLKSLLNHKNNPQNYK*YLEMKEKTPMITSGFGL
GMERLMLFLIGKNDIREVQLIPRETDKEILP
>Mature_351_residues
MLEFPYYAKKYKKGNSITVNFKNFKHDFIKHISSESYAKILTIYSEINFLTYK*MRDNNVKIVNCPITTQSISSPMGLGS
DSLPYKVISKNNPNFEFYLADSMQFHLELFLRTKNVESVGYIAPTFRGENVDERHLHQFNHFEIETKKPLVETKKMIINY
LKYLVKNLVKTHSAELNYFNENNVPRLLK*LNFDQYNLLFTNAIKILDEKFKEGIDRTELLLNINSKGEKFLINYFSDKI
LWLDNFPEINVPFYQKSKDGFAINSDLLMGIGEIVGSGERCFDYNETLKSLLNHKNNPQNYK*YLEMKEKTPMITSGFGL
GMERLMLFLIGKNDIREVQLIPRETDKEILP

Specific function: Unknown

COG id: COG0017

COG function: function code J; Aspartyl/asparaginyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI226491502, Length=325, Percent_Identity=25.8461538461538, Blast_Score=72, Evalue=6e-13,
Organism=Escherichia coli, GI1787161, Length=232, Percent_Identity=26.7241379310345, Blast_Score=77, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6319872, Length=330, Percent_Identity=25.4545454545455, Blast_Score=75, Evalue=2e-14,
Organism=Drosophila melanogaster, GI19921528, Length=258, Percent_Identity=27.5193798449612, Blast_Score=69, Evalue=4e-12,
Organism=Drosophila melanogaster, GI19922416, Length=257, Percent_Identity=29.5719844357977, Blast_Score=68, Evalue=1e-11,

Paralogues:

None

Copy number: 80 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR004522
- InterPro:   IPR002312
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.22 [H]

Molecular weight: Translated: 40700; Mature: 40700

Theoretical pI: Translated: 8.98; Mature: 8.98

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEFPYYAKKYKKGNSITVNFKNFKHDFIKHISSESYAKILTIYSEINFLTYKMRDNNVK
CCCCCHHHHHHCCCCEEEEEEHHHHHHHHHHHCCCHHHHHHHHEECCEEEEEEEECCCEE
IVNCPITTQSISSPMGLGSDSLPYKVISKNNPNFEFYLADSMQFHLELFLRTKNVESVGY
EEECCCCHHHCCCCCCCCCCCCCEEEEECCCCCEEEEEECCCEEEEEEEEEECCCCCCCE
IAPTFRGENVDERHLHQFNHFEIETKKPLVETKKMIINYLKYLVKNLVKTHSAELNYFNE
EECCCCCCCCCHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECC
NNVPRLLKLNFDQYNLLFTNAIKILDEKFKEGIDRTELLLNINSKGEKFLINYFSDKILW
CCCCEEEEECCCCEEEHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEEECCCEEEE
LDNFPEINVPFYQKSKDGFAINSDLLMGIGEIVGSGERCFDYNETLKSLLNHKNNPQNYK
ECCCCCCCCCEEECCCCCEEECHHHHHHHHHHHCCCCCEECHHHHHHHHHHCCCCCCCCE
YLEMKEKTPMITSGFGLGMERLMLFLIGKNDIREVQLIPRETDKEILP
EEEECCCCCEEECCCCCCHHHHHEEEECCCCCEEEEECCCCCCCCCCC
>Mature Secondary Structure
MLEFPYYAKKYKKGNSITVNFKNFKHDFIKHISSESYAKILTIYSEINFLTYKMRDNNVK
CCCCCHHHHHHCCCCEEEEEEHHHHHHHHHHHCCCHHHHHHHHEECCEEEEEEEECCCEE
IVNCPITTQSISSPMGLGSDSLPYKVISKNNPNFEFYLADSMQFHLELFLRTKNVESVGY
EEECCCCHHHCCCCCCCCCCCCCEEEEECCCCCEEEEEECCCEEEEEEEEEECCCCCCCE
IAPTFRGENVDERHLHQFNHFEIETKKPLVETKKMIINYLKYLVKNLVKTHSAELNYFNE
EECCCCCCCCCHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHHHHHHHCCCCEEEECC
NNVPRLLKLNFDQYNLLFTNAIKILDEKFKEGIDRTELLLNINSKGEKFLINYFSDKILW
CCCCEEEEECCCCEEEHHHHHHHHHHHHHHCCCCCEEEEEEECCCCCEEEEEECCCEEEE
LDNFPEINVPFYQKSKDGFAINSDLLMGIGEIVGSGERCFDYNETLKSLLNHKNNPQNYK
ECCCCCCCCCEEECCCCCEEECHHHHHHHHHHHCCCCCEECHHHHHHHHHHCCCCCCCCE
YLEMKEKTPMITSGFGLGMERLMLFLIGKNDIREVQLIPRETDKEILP
EEEECCCCCEEECCCCCCHHHHHEEEECCCCCEEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA