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The map label for this gene is metK

Identifier: 54020517

GI number: 54020517

Start: 553573

End: 554709

Strand: Reverse

Name: metK

Synonym: mhp450

Alternate gene names: 54020517

Gene position: 554709-553573 (Counterclockwise)

Preceding gene: 54020518

Following gene: 54020516

Centisome position: 62.13

GC content: 35.44

Gene sequence:

>1137_bases
ATGTTATCAGCGAAAATTTCTGTTGCTGAATCAGTCGGAAAAGGTCATCCTGATAAAATTTGTGATCAAATTAGCGATGC
TATTCTTGATGAAATTCTTAAACAGGATGAGCACGCACGAGTTGCCGTTGAAACAATGGCATCAAACCGCCTAATTATAA
TTGGCGGGGAAATCTCAACAAGTGGTTATGTCGATCTTGTAAAAATTGCATGAGAGATTTTATTTGAAATTGGTTATAAC
GAAAATGATTTTACAATAATTTCCAATGTAAATCGCCAATCCGGCGAGATAGCTTCCCTTGTAGATCAGAAAAACGGTAC
AATCGGTGCTGGTGATCAAGGGATTGTTTATGGTTTTGCCACTAACGAGTGCCCAAACTTTATGCCCTTAGCACAAAATT
TGGCCAACTTTTTAGTTAAAAAAGCGGAAGAACTCCGGAAAATGGGCGAATTTCAATTTGCATGTGCGGATATGAAATCC
CAAGTTGAGATTCACTATGATCAAAACAGTAAACCAAAAGTTAAAAGTATGCTTATGTCAATCCAACATCAGCAGAATTT
TAACTATCATAATTTTGTTGAATTTGTAATTAACAAAATTATGATTCCAGCAGCAAAAAAATATAATTTAAATATTGACT
TTGAAAAAAAAGTTAACAAATCCGGTGATTTTACAATTGGAGGACCAATTGGTGATACAGGGCTTACCGGTCGAAAACTC
ATAATTGATACTTATGGGGATGCTGCAAAACATGGAGGGGGCGCCTTTAGTGGAAAAGATTATACAAAAGTTGACCGAAG
TGGCGCTTATATTGCCCGGTGAATAGCAAAAAATCTTGTCGCCGCCGGAGTAGCTGCCGAAATCGAGCTACAATTTGCTT
ATGCAATCGGTAATGAATATCCAGAACTAATTAATGTTACTTACCTAAAAAACGCTAAAATTCCAACGGTAAAAATTATT
GAAATTATTAAAAACGTATTTAATCTTCGCCTAGTTAGTTTAATTTCTCGGCTTAATTTGAGGAAACCAATCTACCGAAA
TTTAGCAGTCTATGGTCATTTTGGTCGTGATGAGCTCGATCTTCCCTGAGAAAAACTTGATATGGTTGAAAAAATTAAGG
ATTATCTAAAAATTTAA

Upstream 100 bases:

>100_bases
AAAATTCCCAATAAAATCCTGCGCAAAAATTTTTTCTAAAAAATTTTTTCATTTTTTTAAAAAAAATGGTATAATATAAT
GAAAATTTGAAGGGAAGTAA

Downstream 100 bases:

>100_bases
AATTAAAACTTTTTCTAAATGATTAACTACTTTAAAAAAAGCATTTTTTTATTTGGAAAAAATTAAAAAAATGTTATAAT
GTATATATTATTTTGCCATC

Product: S-adenosylmethionine synthetase

Products: NA

Alternate protein names: AdoMet synthase; MAT; Methionine adenosyltransferase [H]

Number of amino acids: Translated: 378; Mature: 378

Protein sequence:

>378_residues
MLSAKISVAESVGKGHPDKICDQISDAILDEILKQDEHARVAVETMASNRLIIIGGEISTSGYVDLVKIAWEILFEIGYN
ENDFTIISNVNRQSGEIASLVDQKNGTIGAGDQGIVYGFATNECPNFMPLAQNLANFLVKKAEELRKMGEFQFACADMKS
QVEIHYDQNSKPKVKSMLMSIQHQQNFNYHNFVEFVINKIMIPAAKKYNLNIDFEKKVNKSGDFTIGGPIGDTGLTGRKL
IIDTYGDAAKHGGGAFSGKDYTKVDRSGAYIARWIAKNLVAAGVAAEIELQFAYAIGNEYPELINVTYLKNAKIPTVKII
EIIKNVFNLRLVSLISRLNLRKPIYRNLAVYGHFGRDELDLPWEKLDMVEKIKDYLKI

Sequences:

>Translated_378_residues
MLSAKISVAESVGKGHPDKICDQISDAILDEILKQDEHARVAVETMASNRLIIIGGEISTSGYVDLVKIA*EILFEIGYN
ENDFTIISNVNRQSGEIASLVDQKNGTIGAGDQGIVYGFATNECPNFMPLAQNLANFLVKKAEELRKMGEFQFACADMKS
QVEIHYDQNSKPKVKSMLMSIQHQQNFNYHNFVEFVINKIMIPAAKKYNLNIDFEKKVNKSGDFTIGGPIGDTGLTGRKL
IIDTYGDAAKHGGGAFSGKDYTKVDRSGAYIAR*IAKNLVAAGVAAEIELQFAYAIGNEYPELINVTYLKNAKIPTVKII
EIIKNVFNLRLVSLISRLNLRKPIYRNLAVYGHFGRDELDLP*EKLDMVEKIKDYLKI
>Mature_378_residues
MLSAKISVAESVGKGHPDKICDQISDAILDEILKQDEHARVAVETMASNRLIIIGGEISTSGYVDLVKIA*EILFEIGYN
ENDFTIISNVNRQSGEIASLVDQKNGTIGAGDQGIVYGFATNECPNFMPLAQNLANFLVKKAEELRKMGEFQFACADMKS
QVEIHYDQNSKPKVKSMLMSIQHQQNFNYHNFVEFVINKIMIPAAKKYNLNIDFEKKVNKSGDFTIGGPIGDTGLTGRKL
IIDTYGDAAKHGGGAFSGKDYTKVDRSGAYIAR*IAKNLVAAGVAAEIELQFAYAIGNEYPELINVTYLKNAKIPTVKII
EIIKNVFNLRLVSLISRLNLRKPIYRNLAVYGHFGRDELDLP*EKLDMVEKIKDYLKI

Specific function: Catalyzes the formation of S-adenosylmethionine from methionine and ATP. The overall synthetic reaction is composed of two sequential steps, AdoMet formation and the subsequent tripolyphosphate hydrolysis which occurs prior to release of AdoMet from the e

COG id: COG0192

COG function: function code H; S-adenosylmethionine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the AdoMet synthase family [H]

Homologues:

Organism=Homo sapiens, GI5174529, Length=373, Percent_Identity=42.0911528150134, Blast_Score=264, Evalue=8e-71,
Organism=Homo sapiens, GI4557737, Length=372, Percent_Identity=39.5161290322581, Blast_Score=254, Evalue=7e-68,
Organism=Escherichia coli, GI1789311, Length=380, Percent_Identity=42.3684210526316, Blast_Score=305, Evalue=4e-84,
Organism=Caenorhabditis elegans, GI17538494, Length=367, Percent_Identity=41.41689373297, Blast_Score=246, Evalue=1e-65,
Organism=Caenorhabditis elegans, GI17538498, Length=365, Percent_Identity=41.3698630136986, Blast_Score=246, Evalue=2e-65,
Organism=Caenorhabditis elegans, GI25145633, Length=365, Percent_Identity=39.4520547945205, Blast_Score=242, Evalue=2e-64,
Organism=Caenorhabditis elegans, GI17551082, Length=375, Percent_Identity=39.7333333333333, Blast_Score=234, Evalue=4e-62,
Organism=Caenorhabditis elegans, GI32565905, Length=359, Percent_Identity=39.2757660167131, Blast_Score=216, Evalue=1e-56,
Organism=Caenorhabditis elegans, GI32565911, Length=326, Percent_Identity=38.0368098159509, Blast_Score=189, Evalue=2e-48,
Organism=Caenorhabditis elegans, GI25145635, Length=322, Percent_Identity=36.6459627329193, Blast_Score=186, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI32565903, Length=294, Percent_Identity=39.4557823129252, Blast_Score=180, Evalue=1e-45,
Organism=Caenorhabditis elegans, GI32565909, Length=75, Percent_Identity=48, Blast_Score=79, Evalue=5e-15,
Organism=Saccharomyces cerevisiae, GI6320710, Length=383, Percent_Identity=41.77545691906, Blast_Score=246, Evalue=3e-66,
Organism=Saccharomyces cerevisiae, GI6323209, Length=372, Percent_Identity=40.8602150537634, Blast_Score=245, Evalue=8e-66,
Organism=Drosophila melanogaster, GI45552159, Length=365, Percent_Identity=44.9315068493151, Blast_Score=254, Evalue=6e-68,
Organism=Drosophila melanogaster, GI24580529, Length=365, Percent_Identity=44.9315068493151, Blast_Score=254, Evalue=6e-68,
Organism=Drosophila melanogaster, GI24580537, Length=365, Percent_Identity=44.9315068493151, Blast_Score=254, Evalue=6e-68,
Organism=Drosophila melanogaster, GI24580533, Length=365, Percent_Identity=44.9315068493151, Blast_Score=254, Evalue=6e-68,
Organism=Drosophila melanogaster, GI24580535, Length=365, Percent_Identity=44.9315068493151, Blast_Score=254, Evalue=6e-68,
Organism=Drosophila melanogaster, GI24580531, Length=365, Percent_Identity=44.9315068493151, Blast_Score=254, Evalue=6e-68,
Organism=Drosophila melanogaster, GI24580527, Length=365, Percent_Identity=43.8356164383562, Blast_Score=250, Evalue=1e-66,
Organism=Drosophila melanogaster, GI24580525, Length=365, Percent_Identity=43.8356164383562, Blast_Score=250, Evalue=1e-66,
Organism=Drosophila melanogaster, GI24580541, Length=355, Percent_Identity=43.0985915492958, Blast_Score=234, Evalue=8e-62,
Organism=Drosophila melanogaster, GI24580539, Length=166, Percent_Identity=35.5421686746988, Blast_Score=90, Evalue=2e-18,

Paralogues:

None

Copy number: 7,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022631
- InterPro:   IPR022630
- InterPro:   IPR022629
- InterPro:   IPR022628
- InterPro:   IPR002133
- InterPro:   IPR022636 [H]

Pfam domain/function: PF02773 S-AdoMet_synt_C; PF02772 S-AdoMet_synt_M; PF00438 S-AdoMet_synt_N [H]

EC number: =2.5.1.6 [H]

Molecular weight: Translated: 41581; Mature: 41581

Theoretical pI: Translated: 7.20; Mature: 7.20

Prosite motif: PS00376 ADOMET_SYNTHETASE_1 ; PS00377 ADOMET_SYNTHETASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSAKISVAESVGKGHPDKICDQISDAILDEILKQDEHARVAVETMASNRLIIIGGEIST
CCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHEEHEECCCCCEEEEECCCCC
SGYVDLVKIAEILFEIGYNENDFTIISNVNRQSGEIASLVDQKNGTIGAGDQGIVYGFAT
CCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHCCCCCCCCCCCCEEEEEEC
NECPNFMPLAQNLANFLVKKAEELRKMGEFQFACADMKSQVEIHYDQNSKPKVKSMLMSI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCEEEEECCCCCHHHHHHHHHH
QHQQNFNYHNFVEFVINKIMIPAAKKYNLNIDFEKKVNKSGDFTIGGPIGDTGLTGRKLI
HHHCCCCHHHHHHHHHHHHHCCCCCEEECCEEHHHHCCCCCCEEECCCCCCCCCCCCEEE
IDTYGDAAKHGGGAFSGKDYTKVDRSGAYIARIAKNLVAAGVAAEIELQFAYAIGNEYPE
EECCCCHHHCCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHCCEEEEEEEEEEECCCCCHH
LINVTYLKNAKIPTVKIIEIIKNVFNLRLVSLISRLNLRKPIYRNLAVYGHFGRDELDLP
HEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHEEEECCCCCCCCCH
EKLDMVEKIKDYLKI
HHHHHHHHHHHHHCC
>Mature Secondary Structure
MLSAKISVAESVGKGHPDKICDQISDAILDEILKQDEHARVAVETMASNRLIIIGGEIST
CCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHEEHEECCCCCEEEEECCCCC
SGYVDLVKIAEILFEIGYNENDFTIISNVNRQSGEIASLVDQKNGTIGAGDQGIVYGFAT
CCCHHHHHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHHHHCCCCCCCCCCCCEEEEEEC
NECPNFMPLAQNLANFLVKKAEELRKMGEFQFACADMKSQVEIHYDQNSKPKVKSMLMSI
CCCCCHHHHHHHHHHHHHHHHHHHHHHHCHHHHHHCCCCCEEEEECCCCCHHHHHHHHHH
QHQQNFNYHNFVEFVINKIMIPAAKKYNLNIDFEKKVNKSGDFTIGGPIGDTGLTGRKLI
HHHCCCCHHHHHHHHHHHHHCCCCCEEECCEEHHHHCCCCCCEEECCCCCCCCCCCCEEE
IDTYGDAAKHGGGAFSGKDYTKVDRSGAYIARIAKNLVAAGVAAEIELQFAYAIGNEYPE
EECCCCHHHCCCCCCCCCCCCHHCCCCHHHHHHHHHHHHHCCEEEEEEEEEEECCCCCHH
LINVTYLKNAKIPTVKIIEIIKNVFNLRLVSLISRLNLRKPIYRNLAVYGHFGRDELDLP
HEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHEEEECCCCCCCCCH
EKLDMVEKIKDYLKI
HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11353084 [H]