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The map label for this gene is sgaA

Identifier: 54020483

GI number: 54020483

Start: 463356

End: 463835

Strand: Reverse

Name: sgaA

Synonym: mhp386

Alternate gene names: 54020483

Gene position: 463835-463356 (Counterclockwise)

Preceding gene: 54020484

Following gene: 54020482

Centisome position: 51.96

GC content: 32.5

Gene sequence:

>480_bases
ATGTCAGTTAATTTACTTACAAGTCTTAAGGAAAATGATTCAATTCTTATAAATCAGACAGCATCAAACTGACAAGATGC
AGTCAAAATTTCTTGTCAACCATTATTAGATAAAGGTCTAATTAGTACTGAATATGTTCATGCGATAATTCAATCAACAA
TCGAAAATGGGCCTTATTATATTTTAGCCCCTTTTTTAGCTATGCCTCATGCAGAAGCTGGAAAAGGCGTTTTTGGTAAT
GGTTTTTCACTTGTTGTTTTTAATAAACCATTTTATTTTGAAGCTGATAGTCGTCCAGTTCAGATTTTAATTACCCTGGC
AGCTACCAATTCAGATATTCATACATCGGTTGCACTCCCCCAAATTGTTGCCGCTTTTGAAGATGTTGCAAATATTGAAA
AGATTAAAAACGCCCAAAGTAAGGAAGAAATTATTTCACTGCTTGAGAAAGTTGATTTTTCTAAATATTTAGAAAGCTAA

Upstream 100 bases:

>100_bases
TGTTGGCGTAACTAATTTAATGGATGAAAACGAGATAAAAACTGCGCTAACTCCGGTTCTTAAAGAACTAAGTTAGCCAA
AAAATCAAGGATTTTTTTAT

Downstream 100 bases:

>100_bases
ATTTCTTAGGTTTTTCTCTAATTTTTGAGAACCTACAATTTTTGTAGGTTTTTTTTTTTTTTTTTTTTTTCTTAGTCTTT
GAAAAAAATGTTATAATTCT

Product: PTS system, IIA component

Products: protein histidine; sugar phosphate

Alternate protein names: PTS system ascorbate-specific EIIA component [H]

Number of amino acids: Translated: 159; Mature: 158

Protein sequence:

>159_residues
MSVNLLTSLKENDSILINQTASNWQDAVKISCQPLLDKGLISTEYVHAIIQSTIENGPYYILAPFLAMPHAEAGKGVFGN
GFSLVVFNKPFYFEADSRPVQILITLAATNSDIHTSVALPQIVAAFEDVANIEKIKNAQSKEEIISLLEKVDFSKYLES

Sequences:

>Translated_159_residues
MSVNLLTSLKENDSILINQTASN*QDAVKISCQPLLDKGLISTEYVHAIIQSTIENGPYYILAPFLAMPHAEAGKGVFGN
GFSLVVFNKPFYFEADSRPVQILITLAATNSDIHTSVALPQIVAAFEDVANIEKIKNAQSKEEIISLLEKVDFSKYLES
>Mature_158_residues
SVNLLTSLKENDSILINQTASN*QDAVKISCQPLLDKGLISTEYVHAIIQSTIENGPYYILAPFLAMPHAEAGKGVFGNG
FSLVVFNKPFYFEADSRPVQILITLAATNSDIHTSVALPQIVAAFEDVANIEKIKNAQSKEEIISLLEKVDFSKYLES

Specific function: The phosphoenolpyruvate-dependent sugar phosphotransferase system (sugar PTS), a major carbohydrate active -transport system, catalyzes the phosphorylation of incoming sugar substrates concomitantly with their translocation across the cell membrane. This

COG id: COG1762

COG function: function code GT; Phosphotransferase system mannitol/fructose-specific IIA domain (Ntr-type)

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PTS EIIA type-2 domain [H]

Homologues:

Organism=Escherichia coli, GI2367359, Length=151, Percent_Identity=37.7483443708609, Blast_Score=110, Evalue=6e-26,
Organism=Escherichia coli, GI1789302, Length=98, Percent_Identity=43.8775510204082, Blast_Score=89, Evalue=1e-19,
Organism=Escherichia coli, GI1790755, Length=125, Percent_Identity=28.8, Blast_Score=68, Evalue=3e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016152
- InterPro:   IPR002178 [H]

Pfam domain/function: PF00359 PTS_EIIA_2 [H]

EC number: 2.7.1.69

Molecular weight: Translated: 17286; Mature: 17155

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS00372 PTS_EIIA_TYPE_2_HIS ; PS51094 PTS_EIIA_TYPE_2 ; PS00284 SERPIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVNLLTSLKENDSILINQTASNQDAVKISCQPLLDKGLISTEYVHAIIQSTIENGPYYI
CCCCCEECCCCCCCEEEEECCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEE
LAPFLAMPHAEAGKGVFGNGFSLVVFNKPFYFEADSRPVQILITLAATNSDIHTSVALPQ
EECHHHCCCCCCCCCCCCCCEEEEEECCCEEEECCCCCEEEEEEEEECCCCCHHHHHHHH
IVAAFEDVANIEKIKNAQSKEEIISLLEKVDFSKYLES
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHCC
>Mature Secondary Structure 
SVNLLTSLKENDSILINQTASNQDAVKISCQPLLDKGLISTEYVHAIIQSTIENGPYYI
CCCCEECCCCCCCEEEEECCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEE
LAPFLAMPHAEAGKGVFGNGFSLVVFNKPFYFEADSRPVQILITLAATNSDIHTSVALPQ
EECHHHCCCCCCCCCCCCCCEEEEEECCCEEEECCCCCEEEEEEEEECCCCCHHHHHHHH
IVAAFEDVANIEKIKNAQSKEEIISLLEKVDFSKYLES
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: protein N(pi)-phosphohistidine; sugar

Specific reaction: protein N(pi)-phosphohistidine + sugar = protein histidine + sugar phosphate

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8948633 [H]