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The map label for this gene is tig

Identifier: 54020400

GI number: 54020400

Start: 258589

End: 259986

Strand: Reverse

Name: tig

Synonym: mhp233

Alternate gene names: 54020400

Gene position: 259986-258589 (Counterclockwise)

Preceding gene: 54020401

Following gene: 54020399

Centisome position: 29.12

GC content: 30.33

Gene sequence:

>1398_bases
ATGATAAAACGTGAATTTTTACCTGAAAGTGCTGAGCTAAAAATAAAATTAACTGCAGATTCTAAAAAATGAGCTGAATT
CTACCAAAAAGCTGAACAAAAACAGGCAGCAAAAGTTTCTTTACGAGGTTTTCGCAAAGGAAAAGTTCCTCTAGAAAAAG
CAAGAGCTTATTTAAATCCGCAAGCTGTTTTTGAGCTTGCCCTCAGAATGTTTCTTCCCGAACTTGAAAAACAGGCGGCA
ACAAACATAATTGATAGTGACAATGTTATTGAATCACCAATTTTTAACATAGTTAATATGGATAAAAATAATTTAGAAAT
TGAATTTCTTTATCCGGTTTACCCAGAAATTAAATTGCCTGATTATAAGAACCTTAAAACCAAATTTGCCATTAAAAAAA
TAACAAAAGAAGATATCGAGCTGCAAAAACAAAAGCTTTTGGAAGCAAAAGGAAGGTTTATCGAAGTAAATAGACCTGTA
AAAATAGGAGATGTTATTAACTTTAACTTTAAAGGTTTTATTGATGATGAGCCTTTTGATGGCGGAGAAGGAGAAAACTT
TGACCTTCGAATAGGTTCTAATAGTTTTATTGCCGGATTTGAGGAACAACTTGTTGGTCTTGAAATCAAAAAAGAGGCCG
ATATTTATGTAACATTTCCAGAAAATTACCAGGTTCATACCTATGCAAATAAAAAAGCCCGTTTTCGCGTAAAGATTAAT
AAAATAAAGGAAAATCAGCCAGCAAAATTAACAAATGAATTTGTAGCATCACTAAAAATTCAAAATGTTGAAACAATTAG
TCAGTTAGAAGTTTATCTAGAAAATCTTACAGAGCGCGAAAATATCGAAAGAGCAAAAATTGATTTCCAAAAAAATGCAC
TTACTGAAATTGGAGAACAGGTTGAGGTTCCTTTGGCTAAAAAATTAATTAACTTAGAAATTGAGCGACTTAATGAAGTT
TTCCATTCAACACTCAAACAACAAGAGATTCCACTTAAAGAATATTTGAAAATCACTAAATTTACTGAAAAAGATATATA
CGACCAATTCGAAGTAGAAGCAAAAAAATTGCTAAAAAATTCTTTCATTTTCGCCGAAATTGCAAAATTAGAAGGACTTG
TGCCAACTCAGCAAGAATATGAAAGCCATGTTGAAAAGTTAGCAAAATTTACTGGAAAATCAGTTCAGGAAATAAGCGAA
ACTGTTTCTTATAACGAAATTCAGATTAATATCACTAATCAAAAAGTAATTGATAAATTAATCGAGTTCAATCATGAGAC
AAAAGATGAGGAAATAGTTAATAAAAACCAAAATGATAACGAAATTGAACAGGATAAAGAACAAAAGGACAACAATGAAG
AAAAAATAAAGCAAGAAAATAACTTAGAAAACAAATAA

Upstream 100 bases:

>100_bases
ATTTACTTAATTTAGATTATAATTATACTTATTTTTATTTTTTTACTTAGATTTTGAAAATTAACAAGGTTAACCAAATT
AAATTGAGGACGAATTTTTA

Downstream 100 bases:

>100_bases
AAAATTAAAATTGTTCTTCTAAACTTTATTTTTTACAAATTTTTAAAAATTAATAAAATCATAAAAGGAGGAATATGTCA
CGATTTACGCGTTTTTTTTC

Product: trigger factor

Products: peptidylproline (w =0) [C]

Alternate protein names: TF [H]

Number of amino acids: Translated: 465; Mature: 465

Protein sequence:

>465_residues
MIKREFLPESAELKIKLTADSKKWAEFYQKAEQKQAAKVSLRGFRKGKVPLEKARAYLNPQAVFELALRMFLPELEKQAA
TNIIDSDNVIESPIFNIVNMDKNNLEIEFLYPVYPEIKLPDYKNLKTKFAIKKITKEDIELQKQKLLEAKGRFIEVNRPV
KIGDVINFNFKGFIDDEPFDGGEGENFDLRIGSNSFIAGFEEQLVGLEIKKEADIYVTFPENYQVHTYANKKARFRVKIN
KIKENQPAKLTNEFVASLKIQNVETISQLEVYLENLTERENIERAKIDFQKNALTEIGEQVEVPLAKKLINLEIERLNEV
FHSTLKQQEIPLKEYLKITKFTEKDIYDQFEVEAKKLLKNSFIFAEIAKLEGLVPTQQEYESHVEKLAKFTGKSVQEISE
TVSYNEIQINITNQKVIDKLIEFNHETKDEEIVNKNQNDNEIEQDKEQKDNNEEKIKQENNLENK

Sequences:

>Translated_465_residues
MIKREFLPESAELKIKLTADSKK*AEFYQKAEQKQAAKVSLRGFRKGKVPLEKARAYLNPQAVFELALRMFLPELEKQAA
TNIIDSDNVIESPIFNIVNMDKNNLEIEFLYPVYPEIKLPDYKNLKTKFAIKKITKEDIELQKQKLLEAKGRFIEVNRPV
KIGDVINFNFKGFIDDEPFDGGEGENFDLRIGSNSFIAGFEEQLVGLEIKKEADIYVTFPENYQVHTYANKKARFRVKIN
KIKENQPAKLTNEFVASLKIQNVETISQLEVYLENLTERENIERAKIDFQKNALTEIGEQVEVPLAKKLINLEIERLNEV
FHSTLKQQEIPLKEYLKITKFTEKDIYDQFEVEAKKLLKNSFIFAEIAKLEGLVPTQQEYESHVEKLAKFTGKSVQEISE
TVSYNEIQINITNQKVIDKLIEFNHETKDEEIVNKNQNDNEIEQDKEQKDNNEEKIKQENNLENK
>Mature_465_residues
MIKREFLPESAELKIKLTADSKK*AEFYQKAEQKQAAKVSLRGFRKGKVPLEKARAYLNPQAVFELALRMFLPELEKQAA
TNIIDSDNVIESPIFNIVNMDKNNLEIEFLYPVYPEIKLPDYKNLKTKFAIKKITKEDIELQKQKLLEAKGRFIEVNRPV
KIGDVINFNFKGFIDDEPFDGGEGENFDLRIGSNSFIAGFEEQLVGLEIKKEADIYVTFPENYQVHTYANKKARFRVKIN
KIKENQPAKLTNEFVASLKIQNVETISQLEVYLENLTERENIERAKIDFQKNALTEIGEQVEVPLAKKLINLEIERLNEV
FHSTLKQQEIPLKEYLKITKFTEKDIYDQFEVEAKKLLKNSFIFAEIAKLEGLVPTQQEYESHVEKLAKFTGKSVQEISE
TVSYNEIQINITNQKVIDKLIEFNHETKDEEIVNKNQNDNEIEQDKEQKDNNEEKIKQENNLENK

Specific function: Involved in protein export. Acts as a chaperone by maintaining the newly synthesized protein in an open conformation [H]

COG id: COG0544

COG function: function code O; FKBP-type peptidyl-prolyl cis-trans isomerase (trigger factor)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PPIase FKBP-type domain [H]

Homologues:

Organism=Escherichia coli, GI1786640, Length=363, Percent_Identity=24.5179063360882, Blast_Score=94, Evalue=2e-20,

Paralogues:

None

Copy number: 1070 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 300 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 500 Molecules/Cell In: Growth-Phase, Minimal-Media (Based on E. coli). 340 Molecules/Cell In: Growth-Phase, Mini

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001179
- InterPro:   IPR005215
- InterPro:   IPR008880
- InterPro:   IPR008881 [H]

Pfam domain/function: PF00254 FKBP_C; PF05698 Trigger_C; PF05697 Trigger_N [H]

EC number: NA

Molecular weight: Translated: 54004; Mature: 54004

Theoretical pI: Translated: 5.09; Mature: 5.09

Prosite motif: PS50059 FKBP_PPIASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKREFLPESAELKIKLTADSKKAEFYQKAEQKQAAKVSLRGFRKGKVPLEKARAYLNPQ
CCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCHH
AVFELALRMFLPELEKQAATNIIDSDNVIESPIFNIVNMDKNNLEIEFLYPVYPEIKLPD
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCHHHHEECCCCCEEEEEEECCCCCCCCCC
YKNLKTKFAIKKITKEDIELQKQKLLEAKGRFIEVNRPVKIGDVINFNFKGFIDDEPFDG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEECCEEEECCCCCCCCCCCCC
GEGENFDLRIGSNSFIAGFEEQLVGLEIKKEADIYVTFPENYQVHTYANKKARFRVKINK
CCCCCEEEEECCCCCCCCHHHHHCCEEEECCCCEEEECCCCEEEEEEECCCEEEEEEEEE
IKENQPAKLTNEFVASLKIQNVETISQLEVYLENLTERENIERAKIDFQKNALTEIGEQV
CCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHC
EVPLAKKLINLEIERLNEVFHSTLKQQEIPLKEYLKITKFTEKDIYDQFEVEAKKLLKNS
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FIFAEIAKLEGLVPTQQEYESHVEKLAKFTGKSVQEISETVSYNEIQINITNQKVIDKLI
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEECCHHHHHHHH
EFNHETKDEEIVNKNQNDNEIEQDKEQKDNNEEKIKQENNLENK
HCCCCCCCHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHCCCCCC
>Mature Secondary Structure
MIKREFLPESAELKIKLTADSKKAEFYQKAEQKQAAKVSLRGFRKGKVPLEKARAYLNPQ
CCCCCCCCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCHH
AVFELALRMFLPELEKQAATNIIDSDNVIESPIFNIVNMDKNNLEIEFLYPVYPEIKLPD
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHCCHHHHEECCCCCEEEEEEECCCCCCCCCC
YKNLKTKFAIKKITKEDIELQKQKLLEAKGRFIEVNRPVKIGDVINFNFKGFIDDEPFDG
CCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCEEECCEEEECCCCCCCCCCCCC
GEGENFDLRIGSNSFIAGFEEQLVGLEIKKEADIYVTFPENYQVHTYANKKARFRVKINK
CCCCCEEEEECCCCCCCCHHHHHCCEEEECCCCEEEECCCCEEEEEEECCCEEEEEEEEE
IKENQPAKLTNEFVASLKIQNVETISQLEVYLENLTERENIERAKIDFQKNALTEIGEQV
CCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHC
EVPLAKKLINLEIERLNEVFHSTLKQQEIPLKEYLKITKFTEKDIYDQFEVEAKKLLKNS
CCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FIFAEIAKLEGLVPTQQEYESHVEKLAKFTGKSVQEISETVSYNEIQINITNQKVIDKLI
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEEEECCHHHHHHHH
EFNHETKDEEIVNKNQNDNEIEQDKEQKDNNEEKIKQENNLENK
HCCCCCCCHHHHCCCCCCCHHHHHHHHCCCCHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: peptidylproline (w =180) [C]

Specific reaction: peptidylproline (w =180) = peptidylproline (w =0) [C]

General reaction: Isomerases; cis-trans-Isomerases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA