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The map label for this gene is alaS

Identifier: 54020387

GI number: 54020387

Start: 217619

End: 220270

Strand: Reverse

Name: alaS

Synonym: mhp180

Alternate gene names: 54020387

Gene position: 220270-217619 (Counterclockwise)

Preceding gene: 54020388

Following gene: 54020386

Centisome position: 24.67

GC content: 29.83

Gene sequence:

>2652_bases
ATGGAAAAATTATCAGCAAACGAAATAAGACAACTTTGAATTGATTTTTTTCGCGAAAAAAATCATTTTTTTATCGAATC
AAAACCACTTGTTCCCCAAAATGATGACTCACTTCTTTGAATAAATTCAGGAGTTGCCACTTTAAAAGATTATTTTACCG
GCAAAAAAATTCCCCCATCAAAACGACTTGTAAACTCCCAAAAAGCCTTAAGAACAAATGACATTGAAAATGTTGGTCTA
ACCTCGCGCCATCACACCTTATTTGAAATGCTTGGGAATTTTTCAATTGGTGACTATTTTAAAACAGAAGCAATTGACTA
TGCTTATGAATTTTTAACTAAAAAGTTAAAATTAGATCCCAAAAATTTGTTTATTACTTATTATGATGGTGATGATATAA
CTTTTGAAAAATGAAAAAGTTTGGGATTTTCTAATGAAAAATTAATAAAAGGCAGCAAAAAAACAAATTTTTGGGATTTA
GGTCAGGGGCCTTGTGGTCCTTGTAGTGAAATTTATTTTGACCGCGGGCCAAAATTTGATAGTCGAGGGAGCGAACTAAT
CAAAAATGAGATTGAAAATGACCGTTTTATTGAAATCTGAAATATAGTTTTTTCTGAATTTAACAATGATGGCCAGCAAA
ATTATGCACCATTAAAATCAAAAAATATTGATACCGGAGCTGGCTTTGAACGAATTGTTTCGATTTTACAAGATGGCCCA
ACTAATTATGATACCGATCTTTTTCTACCAATTATTGCTGAAATTGAGAAAAACACTGTTTTTCGATACAAAATTGAGAA
TTATTTTCTAAAAAAACCTCGACAAACACAAATTAATAAAAGTTTTCGCATAATTGCCGATCATATTAGAGCAATAACTT
TAGCAATAAATGACGGGGTTCAACCTTCAAATTTACACCGGGGATATATCATTCGCCGCCTGATTCGTCGAGCATATTGG
AATGGAAAAAAACTAGGAATTAGTCACCCTTTTCTTTATAAATTAGTGGAAATTGTTGGCAAAACTTTAGATTATCGTTT
TGATATTCCAACAATTAGCAAAATTATTCTCAATGAAGAAGAAAATTTTGCAAAAACCTTGGAAATAGGGTATAATTTAC
TTGAAAGTCAACTAAAAATTAACAAAAATCAGATAAAGCCTGTGACTGTGTTCAAACTTTTTGAAACTTATGGTTTTCCG
GTTGAACTAACAAAGGAAATTCTTGCTGAAAAGAACATTGATTTTGATCTTAGTCAATTAGTGGAATTTCAAGAAAAACA
TTCACAGATTTCAAGGGCAAAAAAAACTACTGGGATGCAAAAAGTGATTAATTCACTAACTCAAATTAAAGCTAAAATTT
CGGATTTTATTGGTTATCATACTCATCATATTGAGACCAAAATTAGCTTTTTAGCGAATAAAGACGAAGAAGTAGCTGAA
ACAAACGGTGAAAATTTATCTTATGTAATTTTTGAAAAAACTCCTTTTTATGCAACAGCCGGTGGTCAAAAACATGATCA
AGGCTGAATTATTCAAAATAATTCAACAATTGAAATTCTTGATGTTTTTAAAGATAAATTTTTAAATAATATTCATGTCT
TTAAAGGGAAAATTGTTAAAAATCAGCCGGTTTTTCTTAAATTAGATACAAAAAACCGGCTGAATTTAGAGAGAAACCAT
TCAGGCACCCACCTTTTATTTGCCTCACTTAGACAAGAATTTGGCTCTGAGATTAAACAGTTAGGATCTGATAATAATGA
AGATCGCCTAACTTTTGATTTTCCACTTAATCGAAAACCAAGTGATCAAGAAATAAAATCGGTTGAAAATCGCATAAATT
CTTACATAAATCAGAAAATTAAACGGAAATACCTAGTTACAAATCTTGAAGAGGCCCAAAAACTCAATGCAATTATGACA
CTGGAAGAATCAGAATATATGGACCCAAATTCGTTACGGTTGGTGATTTTTGATAAAATCACAACTGATTTATGTGGGGG
AACTCATATTGAAAATACAGAATTACTTGAAAAATTTACAATTTTAAGCTGCCAAAGCAAAGGGTCGGGAATTTATCGAA
TCAGAGCAGTTACTTCCTGGAATAAATATTTTGAGTTTTTGAAAGGAAAAATTCAGGAAATTTTAAGTAAAATTTCCGCA
TTGAAAAACAAAATTAAGAAAATTGAGCCTAATTTTGGCCTAAATTTACCAAATTTAGTTGATTTAGAGCAACAATTTGA
TTACCTAAAAAAAATAGAAGATGATCTAAGAATTTATTATAAAAAACTTCTAAAATCACAATTAAGAATAGCAAAATCGG
AACTTGATGCAAATAAAATAATTGAAATTGGCAAATTTAGTTTTTATTTAGATTTTAACCTTCCACTGCATAATTTAAAG
CAAATAGCCGCCACCTGAAGAGAACAAAATCCAAGAATATCCTTTATTTTAGGTGCTAATTTAGTTAATAATGAATTTTT
AATTATTGTTTCTTCTGCAATTTTAGCTTCTAATCAAATTCTTGAAAAAATTTTAGAAATTTTTACTGGATCAGGAGGTG
GAAATTATAAGATTGCCCAGGGAAAAATTCAAAAAAAACCAGAAAAAGAGGTGTTCATTAAACTTTTATGGGAAAATATA
ACCGAATTTTAG

Upstream 100 bases:

>100_bases
TTTTCATTGACAAAAAAGCGATACATAATAAAAATTTTAAAGGAAAATTGATAAACTATTTAAAAAATAGTTTAAAATTA
TTAAAAAAGAAGGAAAAACT

Downstream 100 bases:

>100_bases
CACTTGATCTTGGCATCAAAACTTGTGGATTTGCAATTTCTGATCAAAACTGAAAAATTTCCTATCCACTTGAACAGTTT
AATTTTAATCGTTATGATTT

Product: alanyl-tRNA synthetase

Products: NA

Alternate protein names: Alanine--tRNA ligase; AlaRS [H]

Number of amino acids: Translated: 883; Mature: 883

Protein sequence:

>883_residues
MEKLSANEIRQLWIDFFREKNHFFIESKPLVPQNDDSLLWINSGVATLKDYFTGKKIPPSKRLVNSQKALRTNDIENVGL
TSRHHTLFEMLGNFSIGDYFKTEAIDYAYEFLTKKLKLDPKNLFITYYDGDDITFEKWKSLGFSNEKLIKGSKKTNFWDL
GQGPCGPCSEIYFDRGPKFDSRGSELIKNEIENDRFIEIWNIVFSEFNNDGQQNYAPLKSKNIDTGAGFERIVSILQDGP
TNYDTDLFLPIIAEIEKNTVFRYKIENYFLKKPRQTQINKSFRIIADHIRAITLAINDGVQPSNLHRGYIIRRLIRRAYW
NGKKLGISHPFLYKLVEIVGKTLDYRFDIPTISKIILNEEENFAKTLEIGYNLLESQLKINKNQIKPVTVFKLFETYGFP
VELTKEILAEKNIDFDLSQLVEFQEKHSQISRAKKTTGMQKVINSLTQIKAKISDFIGYHTHHIETKISFLANKDEEVAE
TNGENLSYVIFEKTPFYATAGGQKHDQGWIIQNNSTIEILDVFKDKFLNNIHVFKGKIVKNQPVFLKLDTKNRLNLERNH
SGTHLLFASLRQEFGSEIKQLGSDNNEDRLTFDFPLNRKPSDQEIKSVENRINSYINQKIKRKYLVTNLEEAQKLNAIMT
LEESEYMDPNSLRLVIFDKITTDLCGGTHIENTELLEKFTILSCQSKGSGIYRIRAVTSWNKYFEFLKGKIQEILSKISA
LKNKIKKIEPNFGLNLPNLVDLEQQFDYLKKIEDDLRIYYKKLLKSQLRIAKSELDANKIIEIGKFSFYLDFNLPLHNLK
QIAATWREQNPRISFILGANLVNNEFLIIVSSAILASNQILEKILEIFTGSGGGNYKIAQGKIQKKPEKEVFIKLLWENI
TEF

Sequences:

>Translated_883_residues
MEKLSANEIRQL*IDFFREKNHFFIESKPLVPQNDDSLL*INSGVATLKDYFTGKKIPPSKRLVNSQKALRTNDIENVGL
TSRHHTLFEMLGNFSIGDYFKTEAIDYAYEFLTKKLKLDPKNLFITYYDGDDITFEK*KSLGFSNEKLIKGSKKTNFWDL
GQGPCGPCSEIYFDRGPKFDSRGSELIKNEIENDRFIEI*NIVFSEFNNDGQQNYAPLKSKNIDTGAGFERIVSILQDGP
TNYDTDLFLPIIAEIEKNTVFRYKIENYFLKKPRQTQINKSFRIIADHIRAITLAINDGVQPSNLHRGYIIRRLIRRAYW
NGKKLGISHPFLYKLVEIVGKTLDYRFDIPTISKIILNEEENFAKTLEIGYNLLESQLKINKNQIKPVTVFKLFETYGFP
VELTKEILAEKNIDFDLSQLVEFQEKHSQISRAKKTTGMQKVINSLTQIKAKISDFIGYHTHHIETKISFLANKDEEVAE
TNGENLSYVIFEKTPFYATAGGQKHDQG*IIQNNSTIEILDVFKDKFLNNIHVFKGKIVKNQPVFLKLDTKNRLNLERNH
SGTHLLFASLRQEFGSEIKQLGSDNNEDRLTFDFPLNRKPSDQEIKSVENRINSYINQKIKRKYLVTNLEEAQKLNAIMT
LEESEYMDPNSLRLVIFDKITTDLCGGTHIENTELLEKFTILSCQSKGSGIYRIRAVTSWNKYFEFLKGKIQEILSKISA
LKNKIKKIEPNFGLNLPNLVDLEQQFDYLKKIEDDLRIYYKKLLKSQLRIAKSELDANKIIEIGKFSFYLDFNLPLHNLK
QIAAT*REQNPRISFILGANLVNNEFLIIVSSAILASNQILEKILEIFTGSGGGNYKIAQGKIQKKPEKEVFIKLLWENI
TEF
>Mature_883_residues
MEKLSANEIRQL*IDFFREKNHFFIESKPLVPQNDDSLL*INSGVATLKDYFTGKKIPPSKRLVNSQKALRTNDIENVGL
TSRHHTLFEMLGNFSIGDYFKTEAIDYAYEFLTKKLKLDPKNLFITYYDGDDITFEK*KSLGFSNEKLIKGSKKTNFWDL
GQGPCGPCSEIYFDRGPKFDSRGSELIKNEIENDRFIEI*NIVFSEFNNDGQQNYAPLKSKNIDTGAGFERIVSILQDGP
TNYDTDLFLPIIAEIEKNTVFRYKIENYFLKKPRQTQINKSFRIIADHIRAITLAINDGVQPSNLHRGYIIRRLIRRAYW
NGKKLGISHPFLYKLVEIVGKTLDYRFDIPTISKIILNEEENFAKTLEIGYNLLESQLKINKNQIKPVTVFKLFETYGFP
VELTKEILAEKNIDFDLSQLVEFQEKHSQISRAKKTTGMQKVINSLTQIKAKISDFIGYHTHHIETKISFLANKDEEVAE
TNGENLSYVIFEKTPFYATAGGQKHDQG*IIQNNSTIEILDVFKDKFLNNIHVFKGKIVKNQPVFLKLDTKNRLNLERNH
SGTHLLFASLRQEFGSEIKQLGSDNNEDRLTFDFPLNRKPSDQEIKSVENRINSYINQKIKRKYLVTNLEEAQKLNAIMT
LEESEYMDPNSLRLVIFDKITTDLCGGTHIENTELLEKFTILSCQSKGSGIYRIRAVTSWNKYFEFLKGKIQEILSKISA
LKNKIKKIEPNFGLNLPNLVDLEQQFDYLKKIEDDLRIYYKKLLKSQLRIAKSELDANKIIEIGKFSFYLDFNLPLHNLK
QIAAT*REQNPRISFILGANLVNNEFLIIVSSAILASNQILEKILEIFTGSGGGNYKIAQGKIQKKPEKEVFIKLLWENI
TEF

Specific function: Catalyzes the attachment of alanine to tRNA(Ala) in a two-step reaction:alanine is first activated by ATP to form Ala- AMP and then transferred to the acceptor end of tRNA(Ala). Also edits incorrectly charged Ser-tRNA(Ala) and Gly-tRNA(Ala) via its editin

COG id: COG0013

COG function: function code J; Alanyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI109148542, Length=864, Percent_Identity=31.712962962963, Blast_Score=354, Evalue=2e-97,
Organism=Homo sapiens, GI38569417, Length=757, Percent_Identity=26.9484808454425, Blast_Score=280, Evalue=4e-75,
Organism=Escherichia coli, GI1789048, Length=764, Percent_Identity=35.9947643979058, Blast_Score=430, Evalue=1e-121,
Organism=Caenorhabditis elegans, GI17506981, Length=772, Percent_Identity=30.8290155440415, Blast_Score=327, Evalue=2e-89,
Organism=Caenorhabditis elegans, GI17536681, Length=748, Percent_Identity=30.4812834224599, Blast_Score=283, Evalue=4e-76,
Organism=Saccharomyces cerevisiae, GI6324911, Length=755, Percent_Identity=32.1854304635762, Blast_Score=364, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24582809, Length=964, Percent_Identity=29.149377593361, Blast_Score=347, Evalue=2e-95,
Organism=Drosophila melanogaster, GI45552267, Length=964, Percent_Identity=29.149377593361, Blast_Score=347, Evalue=2e-95,
Organism=Drosophila melanogaster, GI24658214, Length=927, Percent_Identity=27.0765911542611, Blast_Score=266, Evalue=6e-71,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002318
- InterPro:   IPR018162
- InterPro:   IPR018165
- InterPro:   IPR018164
- InterPro:   IPR023033
- InterPro:   IPR018163
- InterPro:   IPR012947 [H]

Pfam domain/function: PF01411 tRNA-synt_2c; PF07973 tRNA_SAD [H]

EC number: =6.1.1.7 [H]

Molecular weight: Translated: 101131; Mature: 101131

Theoretical pI: Translated: 9.35; Mature: 9.35

Prosite motif: PS50860 AA_TRNA_LIGASE_II_ALA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
1.0 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
1.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKLSANEIRQLIDFFREKNHFFIESKPLVPQNDDSLLINSGVATLKDYFTGKKIPPSKR
CCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCEEEECCHHHHHHHHCCCCCCCHHH
LVNSQKALRTNDIENVGLTSRHHTLFEMLGNFSIGDYFKTEAIDYAYEFLTKKLKLDPKN
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCE
LFITYYDGDDITFEKKSLGFSNEKLIKGSKKTNFWDLGQGPCGPCSEIYFDRGPKFDSRG
EEEEEECCCCEEEEHHHCCCCCCHHCCCCCCCCCEECCCCCCCCCHHHHHCCCCCCCCCC
SELIKNEIENDRFIEINIVFSEFNNDGQQNYAPLKSKNIDTGAGFERIVSILQDGPTNYD
HHHHHHHCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCC
TDLFLPIIAEIEKNTVFRYKIENYFLKKPRQTQINKSFRIIADHIRAITLAINDGVQPSN
CCHHHHHHHHHCCCCEEEEEECHHHHCCCCHHHHCCHHHHHHHHHEEEEEEEECCCCCCC
LHRGYIIRRLIRRAYWNGKKLGISHPFLYKLVEIVGKTLDYRFDIPTISKIILNEEENFA
CHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCEEECCCHHHHHHHCCCHHHH
KTLEIGYNLLESQLKINKNQIKPVTVFKLFETYGFPVELTKEILAEKNIDFDLSQLVEFQ
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHH
EKHSQISRAKKTTGMQKVINSLTQIKAKISDFIGYHTHHIETKISFLANKDEEVAETNGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCC
NLSYVIFEKTPFYATAGGQKHDQGIIQNNSTIEILDVFKDKFLNNIHVFKGKIVKNQPVF
CEEEEEEECCCCEEECCCCCCCCCEEECCCEEEHHHHHHHHHHCCEEEEECEEEECCCEE
LKLDTKNRLNLERNHSGTHLLFASLRQEFGSEIKQLGSDNNEDRLTFDFPLNRKPSDQEI
EEECCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHH
KSVENRINSYINQKIKRKYLVTNLEEAQKLNAIMTLEESEYMDPNSLRLVIFDKITTDLC
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHEEECCCCCCCCCCEEEEEEECHHHHHC
GGTHIENTELLEKFTILSCQSKGSGIYRIRAVTSWNKYFEFLKGKIQEILSKISALKNKI
CCCCCCCHHHHHHHHHEEECCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKIEPNFGLNLPNLVDLEQQFDYLKKIEDDLRIYYKKLLKSQLRIAKSELDANKIIEIGK
HHCCCCCCCCCCCHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHEEECC
FSFYLDFNLPLHNLKQIAATREQNPRISFILGANLVNNEFLIIVSSAILASNQILEKILE
EEEEEEECCCHHHHHHHHHHHCCCCCEEEEEECCEECCCEEEEEEHHHHHHHHHHHHHHH
IFTGSGGGNYKIAQGKIQKKPEKEVFIKLLWENITEF
HHCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MEKLSANEIRQLIDFFREKNHFFIESKPLVPQNDDSLLINSGVATLKDYFTGKKIPPSKR
CCCCCHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCEEEECCHHHHHHHHCCCCCCCHHH
LVNSQKALRTNDIENVGLTSRHHTLFEMLGNFSIGDYFKTEAIDYAYEFLTKKLKLDPKN
HHHHHHHHHCCCCCCCCCCCHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCE
LFITYYDGDDITFEKKSLGFSNEKLIKGSKKTNFWDLGQGPCGPCSEIYFDRGPKFDSRG
EEEEEECCCCEEEEHHHCCCCCCHHCCCCCCCCCEECCCCCCCCCHHHHHCCCCCCCCCC
SELIKNEIENDRFIEINIVFSEFNNDGQQNYAPLKSKNIDTGAGFERIVSILQDGPTNYD
HHHHHHHCCCCCEEEEEEEEEECCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCC
TDLFLPIIAEIEKNTVFRYKIENYFLKKPRQTQINKSFRIIADHIRAITLAINDGVQPSN
CCHHHHHHHHHCCCCEEEEEECHHHHCCCCHHHHCCHHHHHHHHHEEEEEEEECCCCCCC
LHRGYIIRRLIRRAYWNGKKLGISHPFLYKLVEIVGKTLDYRFDIPTISKIILNEEENFA
CHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCEEECCCHHHHHHHCCCHHHH
KTLEIGYNLLESQLKINKNQIKPVTVFKLFETYGFPVELTKEILAEKNIDFDLSQLVEFQ
HHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCHHHHHHHH
EKHSQISRAKKTTGMQKVINSLTQIKAKISDFIGYHTHHIETKISFLANKDEEVAETNGE
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCC
NLSYVIFEKTPFYATAGGQKHDQGIIQNNSTIEILDVFKDKFLNNIHVFKGKIVKNQPVF
CEEEEEEECCCCEEECCCCCCCCCEEECCCEEEHHHHHHHHHHCCEEEEECEEEECCCEE
LKLDTKNRLNLERNHSGTHLLFASLRQEFGSEIKQLGSDNNEDRLTFDFPLNRKPSDQEI
EEECCCCCCCEECCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCCCCCCHHHH
KSVENRINSYINQKIKRKYLVTNLEEAQKLNAIMTLEESEYMDPNSLRLVIFDKITTDLC
HHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHEEECCCCCCCCCCEEEEEEECHHHHHC
GGTHIENTELLEKFTILSCQSKGSGIYRIRAVTSWNKYFEFLKGKIQEILSKISALKNKI
CCCCCCCHHHHHHHHHEEECCCCCCEEEEEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KKIEPNFGLNLPNLVDLEQQFDYLKKIEDDLRIYYKKLLKSQLRIAKSELDANKIIEIGK
HHCCCCCCCCCCCHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHEEECC
FSFYLDFNLPLHNLKQIAATREQNPRISFILGANLVNNEFLIIVSSAILASNQILEKILE
EEEEEEECCCHHHHHHHHHHHCCCCCEEEEEECCEECCCEEEEEEHHHHHHHHHHHHHHH
IFTGSGGGNYKIAQGKIQKKPEKEVFIKLLWENITEF
HHCCCCCCCEEEECCCCCCCCHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA