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The map label for this gene is lysS

Identifier: 54020381

GI number: 54020381

Start: 211544

End: 213214

Strand: Reverse

Name: lysS

Synonym: mhp174

Alternate gene names: 54020381

Gene position: 213214-211544 (Counterclockwise)

Preceding gene: 54020382

Following gene: 54020380

Centisome position: 23.88

GC content: 30.04

Gene sequence:

>1671_bases
ATGAGATTCTTAATCAAATTCTTAAATCCCAACCAGAAACTAGTCCAGAAACAGGAACAGATTCAAGTCAAGAAAATTTC
TAATTACAAAAAAACTCTATTTTTACTAACTTTTATAAATTATTTTTGAAATTTGCAAAAGTTAGTAAAAATTAAAAAGT
TTTTTCAAAAGAAGCTTTTTAATTTTATTAAGGTGATGAAAATTATGTCAAAACTAAATGATCAACAACAATTTCGCCGC
GATAAGCTTAAAAATTTAGTTAAAAATGGTTTTAATTTCCCTAGTTCAACTTTTGAGCATGATAATTTAGTAGAAATAAA
TGAAAAATTTAGTCAAAAATCGAAGCAATTTTTCCTTGAAAATCAAGTAAAAATTGCTTTTGCTGGTCGACTTATTCGCC
AACGCGGCCCATTTTTTATAATTTTTAGTCAAAACTTGCAATTTCAAGCTTATATTTCAAAAGAATTTCAAAAAAAAAAT
GAATTTATCTTTGCAAATTTAGATCTTGGTGATATAATTGAGGTTTCAGGTTATCTTTTCAAGACGCAAACTGGTCAACT
TAGTATTAAAGTTAATAATTTTTCTCTGCTTACAAAGTCCTTACACCCTTTACCAGATCAGTATTATGGTATTGAAAATC
CAGATGAGAAATACAGGAAACGTTATCTTGACCTGCTTGTAAATTCAGAATCTGCAAAAACTTTTCGACTTAGAAGTAAA
ATTATCTCCTTAATCCGTACATTTTTTGATTCCCAAGGTTTTTTAGAAGTTGATACTCCCGTTTTACAGCCAGTTTTAGG
CGGTGCATCAGCAAAACCTTTTATTACTTATTATAATTCTCTTAGTCAAAATTTTTATCTCCGAATTGCCACGGAATTAC
CGCTGAAAAAATTATTAGTTGCCGGTTTTGATCGTGTCTATGAAATTGGTAAAATTTTTCGTAATGAGGGTTTTGATTCA
ACTCATAATCCTGAATTCACCTCAATTGAATTTTATCAGGCTTATGCAAATTTAGAGAAAATAATGGATCAAACCGAAAA
TTTATTTCGTTTTTTATTTGAAAAATTAAATCTTGATCCGGCAAATTTTGATTTTTCAAATAAAAAAATTAATTTCTTGG
AAAAATTTGCCCGTTATGATATGATTGAGATTACTTCAAAATTAATGAACTTCGATTTAAAAAGTGCTAATTTTGCTGAT
TTAGTCGAAAAAGCAAAAAAAGAAGGGGTGAAGATCGAGCCATTTTTCAAAAAAGGGCATCTAATCAACAAATTTTTTGA
AAAATTTGTTGAACCAACACTAATAAATCCAACTTTTATCATAGGTCATCCAATTGAAATTTCCCCGCTAGCCAAGTCAA
ATCCAAACAATCCTAATTTTACTCTCAGGGCCGAATTATTTATCTGTGGTAAAGAATTTGCGAATATGTTTGATGAATTA
AATGATCCAATTGACCAATTAAGTCGTTTTCAAGCCCAAATTATCGAAAAAAATCAGGGAAATCAAGAAGCAAGTGAGAT
CGACAATGAATTTGTTCAAGCACTTGAATATGGAATGCCACCCGCCGGGGGTTGTGGCATTGGCATTGACAGATTGACAA
TGCTTTTAACAAAGAATGAATCAATCCGTGAGGTAATTTTATTCCCACAGCTAAAACCAAAAAAGGACTAA

Upstream 100 bases:

>100_bases
GAAAAAGAGACAATTGTTTATGAGGAAATCCAACAATTAGCCGAGACATTAATGCCGCTTCCGGAAGTTAGTAAAACCGA
AAATAATGCTATAAAACCCG

Downstream 100 bases:

>100_bases
AATGAAACTATTTTTTGCTTTTGACCTGGATGGAACTTTATTACGTTATGATAATACAATTCATCCTGAAAATGTAGAGA
TTTTAAAAAAACTCTATGAA

Product: lysyl-tRNA synthetase

Products: NA

Alternate protein names: Lysine--tRNA ligase; LysRS [H]

Number of amino acids: Translated: 556; Mature: 556

Protein sequence:

>556_residues
MRFLIKFLNPNQKLVQKQEQIQVKKISNYKKTLFLLTFINYFWNLQKLVKIKKFFQKKLFNFIKVMKIMSKLNDQQQFRR
DKLKNLVKNGFNFPSSTFEHDNLVEINEKFSQKSKQFFLENQVKIAFAGRLIRQRGPFFIIFSQNLQFQAYISKEFQKKN
EFIFANLDLGDIIEVSGYLFKTQTGQLSIKVNNFSLLTKSLHPLPDQYYGIENPDEKYRKRYLDLLVNSESAKTFRLRSK
IISLIRTFFDSQGFLEVDTPVLQPVLGGASAKPFITYYNSLSQNFYLRIATELPLKKLLVAGFDRVYEIGKIFRNEGFDS
THNPEFTSIEFYQAYANLEKIMDQTENLFRFLFEKLNLDPANFDFSNKKINFLEKFARYDMIEITSKLMNFDLKSANFAD
LVEKAKKEGVKIEPFFKKGHLINKFFEKFVEPTLINPTFIIGHPIEISPLAKSNPNNPNFTLRAELFICGKEFANMFDEL
NDPIDQLSRFQAQIIEKNQGNQEASEIDNEFVQALEYGMPPAGGCGIGIDRLTMLLTKNESIREVILFPQLKPKKD

Sequences:

>Translated_556_residues
MRFLIKFLNPNQKLVQKQEQIQVKKISNYKKTLFLLTFINYF*NLQKLVKIKKFFQKKLFNFIKVMKIMSKLNDQQQFRR
DKLKNLVKNGFNFPSSTFEHDNLVEINEKFSQKSKQFFLENQVKIAFAGRLIRQRGPFFIIFSQNLQFQAYISKEFQKKN
EFIFANLDLGDIIEVSGYLFKTQTGQLSIKVNNFSLLTKSLHPLPDQYYGIENPDEKYRKRYLDLLVNSESAKTFRLRSK
IISLIRTFFDSQGFLEVDTPVLQPVLGGASAKPFITYYNSLSQNFYLRIATELPLKKLLVAGFDRVYEIGKIFRNEGFDS
THNPEFTSIEFYQAYANLEKIMDQTENLFRFLFEKLNLDPANFDFSNKKINFLEKFARYDMIEITSKLMNFDLKSANFAD
LVEKAKKEGVKIEPFFKKGHLINKFFEKFVEPTLINPTFIIGHPIEISPLAKSNPNNPNFTLRAELFICGKEFANMFDEL
NDPIDQLSRFQAQIIEKNQGNQEASEIDNEFVQALEYGMPPAGGCGIGIDRLTMLLTKNESIREVILFPQLKPKKD
>Mature_556_residues
MRFLIKFLNPNQKLVQKQEQIQVKKISNYKKTLFLLTFINYF*NLQKLVKIKKFFQKKLFNFIKVMKIMSKLNDQQQFRR
DKLKNLVKNGFNFPSSTFEHDNLVEINEKFSQKSKQFFLENQVKIAFAGRLIRQRGPFFIIFSQNLQFQAYISKEFQKKN
EFIFANLDLGDIIEVSGYLFKTQTGQLSIKVNNFSLLTKSLHPLPDQYYGIENPDEKYRKRYLDLLVNSESAKTFRLRSK
IISLIRTFFDSQGFLEVDTPVLQPVLGGASAKPFITYYNSLSQNFYLRIATELPLKKLLVAGFDRVYEIGKIFRNEGFDS
THNPEFTSIEFYQAYANLEKIMDQTENLFRFLFEKLNLDPANFDFSNKKINFLEKFARYDMIEITSKLMNFDLKSANFAD
LVEKAKKEGVKIEPFFKKGHLINKFFEKFVEPTLINPTFIIGHPIEISPLAKSNPNNPNFTLRAELFICGKEFANMFDEL
NDPIDQLSRFQAQIIEKNQGNQEASEIDNEFVQALEYGMPPAGGCGIGIDRLTMLLTKNESIREVILFPQLKPKKD

Specific function: Also Can Synthesize A Number Of Adenyl Dinucleotides (In Particular Appppa). These Dinucleotides Have Been Proposed To Act As Modulators Of The Heat-Shock Response And Stress Response. [C]

COG id: COG1190

COG function: function code J; Lysyl-tRNA synthetase (class II)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI194272210, Length=504, Percent_Identity=39.0873015873016, Blast_Score=339, Evalue=3e-93,
Organism=Homo sapiens, GI5031815, Length=504, Percent_Identity=39.0873015873016, Blast_Score=339, Evalue=3e-93,
Organism=Homo sapiens, GI45439306, Length=354, Percent_Identity=25.7062146892655, Blast_Score=98, Evalue=2e-20,
Organism=Escherichia coli, GI1790571, Length=498, Percent_Identity=42.1686746987952, Blast_Score=394, Evalue=1e-111,
Organism=Escherichia coli, GI1789256, Length=497, Percent_Identity=41.046277665996, Blast_Score=387, Evalue=1e-108,
Organism=Escherichia coli, GI87082379, Length=320, Percent_Identity=30, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI1788173, Length=199, Percent_Identity=30.1507537688442, Blast_Score=84, Evalue=2e-17,
Organism=Caenorhabditis elegans, GI17535925, Length=479, Percent_Identity=37.5782881002088, Blast_Score=332, Evalue=3e-91,
Organism=Caenorhabditis elegans, GI71994340, Length=479, Percent_Identity=37.5782881002088, Blast_Score=332, Evalue=3e-91,
Organism=Caenorhabditis elegans, GI17535927, Length=479, Percent_Identity=37.5782881002088, Blast_Score=332, Evalue=4e-91,
Organism=Caenorhabditis elegans, GI32566633, Length=243, Percent_Identity=26.3374485596708, Blast_Score=73, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6320242, Length=461, Percent_Identity=41.4316702819957, Blast_Score=353, Evalue=3e-98,
Organism=Saccharomyces cerevisiae, GI6324256, Length=417, Percent_Identity=35.2517985611511, Blast_Score=212, Evalue=2e-55,
Organism=Saccharomyces cerevisiae, GI6323011, Length=353, Percent_Identity=26.9121813031161, Blast_Score=97, Evalue=5e-21,
Organism=Drosophila melanogaster, GI24640851, Length=457, Percent_Identity=40.4814004376368, Blast_Score=341, Evalue=1e-93,
Organism=Drosophila melanogaster, GI24640849, Length=457, Percent_Identity=40.4814004376368, Blast_Score=340, Evalue=1e-93,
Organism=Drosophila melanogaster, GI17136276, Length=347, Percent_Identity=25.0720461095101, Blast_Score=95, Evalue=1e-19,
Organism=Drosophila melanogaster, GI24584738, Length=247, Percent_Identity=26.3157894736842, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004364
- InterPro:   IPR018150
- InterPro:   IPR006195
- InterPro:   IPR002313
- InterPro:   IPR018149
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004365 [H]

Pfam domain/function: PF00152 tRNA-synt_2; PF01336 tRNA_anti [H]

EC number: =6.1.1.6 [H]

Molecular weight: Translated: 64861; Mature: 64861

Theoretical pI: Translated: 9.96; Mature: 9.96

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRFLIKFLNPNQKLVQKQEQIQVKKISNYKKTLFLLTFINYFNLQKLVKIKKFFQKKLFN
CCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FIKVMKIMSKLNDQQQFRRDKLKNLVKNGFNFPSSTFEHDNLVEINEKFSQKSKQFFLEN
HHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHC
QVKIAFAGRLIRQRGPFFIIFSQNLQFQAYISKEFQKKNEFIFANLDLGDIIEVSGYLFK
CEEHHHHHHHHHCCCCEEEEEECCCEEEHHHHHHHHCCCCEEEEECCHHHHHHHCCEEEE
TQTGQLSIKVNNFSLLTKSLHPLPDQYYGIENPDEKYRKRYLDLLVNSESAKTFRLRSKI
ECCCEEEEEECCHHHHHHHCCCCCHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHH
ISLIRTFFDSQGFLEVDTPVLQPVLGGASAKPFITYYNSLSQNFYLRIATELPLKKLLVA
HHHHHHHHCCCCCEEECCHHHHHHHCCCCCCCHHHHHHHCCCCEEEEEECCCCHHHHHHH
GFDRVYEIGKIFRNEGFDSTHNPEFTSIEFYQAYANLEKIMDQTENLFRFLFEKLNLDPA
HHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
NFDFSNKKINFLEKFARYDMIEITSKLMNFDLKSANFADLVEKAKKEGVKIEPFFKKGHL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEECCHHHCCHH
INKFFEKFVEPTLINPTFIIGHPIEISPLAKSNPNNPNFTLRAELFICGKEFANMFDELN
HHHHHHHHCCCCCCCCEEEECCCEEECCCCCCCCCCCCEEEEEEEEEECHHHHHHHHHHC
DPIDQLSRFQAQIIEKNQGNQEASEIDNEFVQALEYGMPPAGGCGIGIDRLTMLLTKNES
CHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCC
IREVILFPQLKPKKD
HHHEEECCCCCCCCC
>Mature Secondary Structure
MRFLIKFLNPNQKLVQKQEQIQVKKISNYKKTLFLLTFINYFNLQKLVKIKKFFQKKLFN
CCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FIKVMKIMSKLNDQQQFRRDKLKNLVKNGFNFPSSTFEHDNLVEINEKFSQKSKQFFLEN
HHHHHHHHHHHCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHC
QVKIAFAGRLIRQRGPFFIIFSQNLQFQAYISKEFQKKNEFIFANLDLGDIIEVSGYLFK
CEEHHHHHHHHHCCCCEEEEEECCCEEEHHHHHHHHCCCCEEEEECCHHHHHHHCCEEEE
TQTGQLSIKVNNFSLLTKSLHPLPDQYYGIENPDEKYRKRYLDLLVNSESAKTFRLRSKI
ECCCEEEEEECCHHHHHHHCCCCCHHHCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHH
ISLIRTFFDSQGFLEVDTPVLQPVLGGASAKPFITYYNSLSQNFYLRIATELPLKKLLVA
HHHHHHHHCCCCCEEECCHHHHHHHCCCCCCCHHHHHHHCCCCEEEEEECCCCHHHHHHH
GFDRVYEIGKIFRNEGFDSTHNPEFTSIEFYQAYANLEKIMDQTENLFRFLFEKLNLDPA
HHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCC
NFDFSNKKINFLEKFARYDMIEITSKLMNFDLKSANFADLVEKAKKEGVKIEPFFKKGHL
CCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEECCHHHCCHH
INKFFEKFVEPTLINPTFIIGHPIEISPLAKSNPNNPNFTLRAELFICGKEFANMFDELN
HHHHHHHHCCCCCCCCEEEECCCEEECCCCCCCCCCCCEEEEEEEEEECHHHHHHHHHHC
DPIDQLSRFQAQIIEKNQGNQEASEIDNEFVQALEYGMPPAGGCGIGIDRLTMLLTKNES
CHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHCCCCC
IREVILFPQLKPKKD
HHHEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA