Click here to switch to the map view.

The map label for this gene is cof [H]

Identifier: 54020380

GI number: 54020380

Start: 210712

End: 211542

Strand: Reverse

Name: cof [H]

Synonym: mhp173

Alternate gene names: 54020380

Gene position: 211542-210712 (Counterclockwise)

Preceding gene: 54020381

Following gene: 54020379

Centisome position: 23.7

GC content: 31.41

Gene sequence:

>831_bases
ATGAAACTATTTTTTGCTTTTGACCTGGATGGAACTTTATTACGTTATGATAATACAATTCATCCTGAAAATGTAGAGAT
TTTAAAAAAACTCTATGAATTAGGCCACTTTTTAGCTCTAGCCACCGGCCGAGGACTTGCTGGCTGCCTTGATTTAGCCA
AAAAATATCCTTATTTTCATTATCTAGTTTCAAATAATGGAACCTTAGTCCATGATACTAAAACCCAAAAAACCATCAAT
AATGGTAGTCTTAGCAAGGAAATTATCCTTGATTTGATCAAAGATTGTAAAGCAACTGATTCGATTTGTGCTTTCTCGAG
TCTTAATAACCTTTTTGAATTTTCAAGTACAAACAATCATCCCTGACTAAAAAAACAGAAAATAATGGACCTGCACTTCT
ATGAAAAAGTAGATCAGGATAAATTATATGAAATCATCGAAAAAGAGGAAATTACCCAAGTTGCCTTCCGTAATGATATT
CCAGTGATTGCTGAACTTTATAAAAAATGGTCAAAAAAACTAAAAAATATCTATAAAGTAACAATTACAAACCGTATTTT
TCTTGACATAAACCCTTTAAATGTTGATAAAGCTAACGGAATTAAGATGCTATTAGAAAAAAATAACCTAAAACCTGACC
AGTTAATCGCTTTTGGTGATAGTTCAAATGATTATTTTATGGTAAAATTAGCTCGTTTTGGTTATGCAATGGAAGATTCA
ACCCCTGATTTGTTAAGCGTTGCTTATCAAAAAATTGGCAATTGTAACTCCGGATCAATTGCCAAAACGATAAAATCACT
TTTAGAAAAACAAGAAGAGCTATTTTCCTAG

Upstream 100 bases:

>100_bases
TGTGGCATTGGCATTGACAGATTGACAATGCTTTTAACAAAGAATGAATCAATCCGTGAGGTAATTTTATTCCCACAGCT
AAAACCAAAAAAGGACTAAA

Downstream 100 bases:

>100_bases
GCAAAATTTTGAAAAAATTGAATCAAGAAGATTTTCATCATCAACTCGGCCAAAAATAGTTTTTAATTCTTTTCAGGCAA
TTCTGAGATCAACAATTGCA

Product: hypothetical protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 276; Mature: 276

Protein sequence:

>276_residues
MKLFFAFDLDGTLLRYDNTIHPENVEILKKLYELGHFLALATGRGLAGCLDLAKKYPYFHYLVSNNGTLVHDTKTQKTIN
NGSLSKEIILDLIKDCKATDSICAFSSLNNLFEFSSTNNHPWLKKQKIMDLHFYEKVDQDKLYEIIEKEEITQVAFRNDI
PVIAELYKKWSKKLKNIYKVTITNRIFLDINPLNVDKANGIKMLLEKNNLKPDQLIAFGDSSNDYFMVKLARFGYAMEDS
TPDLLSVAYQKIGNCNSGSIAKTIKSLLEKQEELFS

Sequences:

>Translated_276_residues
MKLFFAFDLDGTLLRYDNTIHPENVEILKKLYELGHFLALATGRGLAGCLDLAKKYPYFHYLVSNNGTLVHDTKTQKTIN
NGSLSKEIILDLIKDCKATDSICAFSSLNNLFEFSSTNNHP*LKKQKIMDLHFYEKVDQDKLYEIIEKEEITQVAFRNDI
PVIAELYKKWSKKLKNIYKVTITNRIFLDINPLNVDKANGIKMLLEKNNLKPDQLIAFGDSSNDYFMVKLARFGYAMEDS
TPDLLSVAYQKIGNCNSGSIAKTIKSLLEKQEELFS
>Mature_276_residues
MKLFFAFDLDGTLLRYDNTIHPENVEILKKLYELGHFLALATGRGLAGCLDLAKKYPYFHYLVSNNGTLVHDTKTQKTIN
NGSLSKEIILDLIKDCKATDSICAFSSLNNLFEFSSTNNHP*LKKQKIMDLHFYEKVDQDKLYEIIEKEEITQVAFRNDI
PVIAELYKKWSKKLKNIYKVTITNRIFLDINPLNVDKANGIKMLLEKNNLKPDQLIAFGDSSNDYFMVKLARFGYAMEDS
TPDLLSVAYQKIGNCNSGSIAKTIKSLLEKQEELFS

Specific function: Catalyzes the hydrolysis of 4-amino-2-methyl-5- hydroxymethylpyrimidine pyrophosphate (HMP-PP) to 4-amino-2- methyl-5-hydroxymethylpyrimidine phosphate (HMP-P) [H]

COG id: COG0561

COG function: function code R; Predicted hydrolases of the HAD superfamily

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the HAD-like hydrolase superfamily. Cof family [H]

Homologues:

Organism=Escherichia coli, GI2367265, Length=257, Percent_Identity=24.5136186770428, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI1787043, Length=273, Percent_Identity=24.5421245421245, Blast_Score=69, Evalue=4e-13,
Organism=Escherichia coli, GI48994981, Length=245, Percent_Identity=26.530612244898, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023214
- InterPro:   IPR013200
- InterPro:   IPR006379
- InterPro:   IPR000150 [H]

Pfam domain/function: PF08282 Hydrolase_3 [H]

EC number: NA

Molecular weight: Translated: 31425; Mature: 31425

Theoretical pI: Translated: 7.96; Mature: 7.96

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLFFAFDLDGTLLRYDNTIHPENVEILKKLYELGHFLALATGRGLAGCLDLAKKYPYFH
CEEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCEEE
YLVSNNGTLVHDTKTQKTINNGSLSKEIILDLIKDCKATDSICAFSSLNNLFEFSSTNNH
EEEECCCEEEECCCHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCC
PLKKQKIMDLHFYEKVDQDKLYEIIEKEEITQVAFRNDIPVIAELYKKWSKKLKNIYKVT
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEEEE
ITNRIFLDINPLNVDKANGIKMLLEKNNLKPDQLIAFGDSSNDYFMVKLARFGYAMEDST
EEEEEEEEECCCCCCCCCCEEEEEECCCCCHHHEEEECCCCCCEEEEEEHHHCCCCCCCC
PDLLSVAYQKIGNCNSGSIAKTIKSLLEKQEELFS
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MKLFFAFDLDGTLLRYDNTIHPENVEILKKLYELGHFLALATGRGLAGCLDLAKKYPYFH
CEEEEEECCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCEEE
YLVSNNGTLVHDTKTQKTINNGSLSKEIILDLIKDCKATDSICAFSSLNNLFEFSSTNNH
EEEECCCEEEECCCHHHHCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHCCCCCCCC
PLKKQKIMDLHFYEKVDQDKLYEIIEKEEITQVAFRNDIPVIAELYKKWSKKLKNIYKVT
CCHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHEEEE
ITNRIFLDINPLNVDKANGIKMLLEKNNLKPDQLIAFGDSSNDYFMVKLARFGYAMEDST
EEEEEEEEECCCCCCCCCCEEEEEECCCCCHHHEEEECCCCCCEEEEEEHHHCCCCCCCC
PDLLSVAYQKIGNCNSGSIAKTIKSLLEKQEELFS
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA