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The map label for this gene is kpnIM [H]

Identifier: 54020181

GI number: 54020181

Start: 478396

End: 479658

Strand: Direct

Name: kpnIM [H]

Synonym: mhp400

Alternate gene names: 54020181

Gene position: 478396-479658 (Clockwise)

Preceding gene: 54020180

Following gene: 54020182

Centisome position: 53.59

GC content: 27.55

Gene sequence:

>1263_bases
ATGAATGAAAGATTAAAATTAGCAAGGGATCTTCTAAAAGAGGATGGAATAATTTTTGTCTCAATCGATGATAATCAGCA
AGCTTATTTAAAAGTTTTAATGGATGAAATTTTTGGTGAGGAAAATTTTGTTGCTAATTTTCCATTTATTTCAAATTTAT
CGGGTCGCCAAGTAAATACAAATTTGGCACTTTGCCATGAATATTTATTGCTCTATAAAAAAAATAATTTCATATTTAAT
AAAATTGATAAAGATTATGCCAATAACTTTATGCCTATTGTTTATCCCGAGAGATCTAATGTGATTTTCAATGAAGACCC
TGATGTAAATAAGGAAATTTATGATAATAGCCCAGAGCAAAAAGAAGATAACTCCACTCAATACATTTTGAGAGATAATT
TAGAAAATTCAAATGGTGAGTTTAACCCTAAAACTCGTCCTAATTTATTTTTCCCAATTTACGCGCGGAAAATTAATGAG
AAAAATAAATTATGAGATATTACGGTGGAAAAGCCTGATTCAAAACATGATTTTATTGAAATTTGACCTCGTAAAAATAG
CAAAAATATTCAATTAGTTTGGCGTTGAGGTAAGAAGAAAATAATGAACCAAATTAATGATCTTGTTATTGTGGAGCCAA
GGGATAAAAATGATAAAAATTATAGAATAAAAGTAAAAATATTTGATCAATCCTACACCGTTAAATCATTTATTTTAGGT
AAAAAATTCAATAATAAGTCCGCTACTAAAGAATTAAACAAGATATTTAGCGAATCTTCGGAAAAGGTTTTCAATTTTCC
TAAATCCACTGCGTTAATAGAATATCTAATTAATTTACACCCTAATAAAAATGCAAGAGTTCTTGATTTTTTTGCCGGAA
GTGGGACAACTGGTCAGGCAGTTTTAGAACTAAATTCCCAAGATGGTGGCACTCGCAGTTTTACTCTGATAACTAATAAT
CAAAATAAAATTGCCGAAAATGTAACTTATGAAAGACTTTTTCGAATAAATCACGGTTTTGGAACAAAAAAAGAACAAAA
TTTTGACTGGATCAAGAAAAATCAACCTTATAAAACTAATTTAGATGTTTTTCGAATTAATTATTTTAATACAGAAATTT
TTAACAAGGAAAATGATGTTAATTTATTAGTTAAAAAATTAGAAAAAATGTTAACTAATTTTGGAATTTCTAGCAAAAAT
AGCATAAATCTAATTGAATATCTTAACTATCTCCTTGCGCTTTTGCCCCAAAAAAAAGGATAA

Upstream 100 bases:

>100_bases
TAAAAATGATGGGAATTCAATTGCTAATGAAAAAGATGATATAAAAGCAAGTAAATTTATTTATCGTGATAAATTTTCGC
GTAATGGCTGGCTAAATTTA

Downstream 100 bases:

>100_bases
AAAATAATATATTTAATAGATGCCCAAAAAAACGTTGTTAAAAAATTATTTAAAAAAGCGCGTAATTCTCTTAGTAACGA
AAAAAAGAAGCAATTTATTT

Product: hypothetical protein

Products: NA

Alternate protein names: M.KpnI; Adenine-specific methyltransferase KpnI [H]

Number of amino acids: Translated: 420; Mature: 420

Protein sequence:

>420_residues
MNERLKLARDLLKEDGIIFVSIDDNQQAYLKVLMDEIFGEENFVANFPFISNLSGRQVNTNLALCHEYLLLYKKNNFIFN
KIDKDYANNFMPIVYPERSNVIFNEDPDVNKEIYDNSPEQKEDNSTQYILRDNLENSNGEFNPKTRPNLFFPIYARKINE
KNKLWDITVEKPDSKHDFIEIWPRKNSKNIQLVWRWGKKKIMNQINDLVIVEPRDKNDKNYRIKVKIFDQSYTVKSFILG
KKFNNKSATKELNKIFSESSEKVFNFPKSTALIEYLINLHPNKNARVLDFFAGSGTTGQAVLELNSQDGGTRSFTLITNN
QNKIAENVTYERLFRINHGFGTKKEQNFDWIKKNQPYKTNLDVFRINYFNTEIFNKENDVNLLVKKLEKMLTNFGISSKN
SINLIEYLNYLLALLPQKKG

Sequences:

>Translated_420_residues
MNERLKLARDLLKEDGIIFVSIDDNQQAYLKVLMDEIFGEENFVANFPFISNLSGRQVNTNLALCHEYLLLYKKNNFIFN
KIDKDYANNFMPIVYPERSNVIFNEDPDVNKEIYDNSPEQKEDNSTQYILRDNLENSNGEFNPKTRPNLFFPIYARKINE
KNKL*DITVEKPDSKHDFIEI*PRKNSKNIQLVWR*GKKKIMNQINDLVIVEPRDKNDKNYRIKVKIFDQSYTVKSFILG
KKFNNKSATKELNKIFSESSEKVFNFPKSTALIEYLINLHPNKNARVLDFFAGSGTTGQAVLELNSQDGGTRSFTLITNN
QNKIAENVTYERLFRINHGFGTKKEQNFDWIKKNQPYKTNLDVFRINYFNTEIFNKENDVNLLVKKLEKMLTNFGISSKN
SINLIEYLNYLLALLPQKKG
>Mature_420_residues
MNERLKLARDLLKEDGIIFVSIDDNQQAYLKVLMDEIFGEENFVANFPFISNLSGRQVNTNLALCHEYLLLYKKNNFIFN
KIDKDYANNFMPIVYPERSNVIFNEDPDVNKEIYDNSPEQKEDNSTQYILRDNLENSNGEFNPKTRPNLFFPIYARKINE
KNKL*DITVEKPDSKHDFIEI*PRKNSKNIQLVWR*GKKKIMNQINDLVIVEPRDKNDKNYRIKVKIFDQSYTVKSFILG
KKFNNKSATKELNKIFSESSEKVFNFPKSTALIEYLINLHPNKNARVLDFFAGSGTTGQAVLELNSQDGGTRSFTLITNN
QNKIAENVTYERLFRINHGFGTKKEQNFDWIKKNQPYKTNLDVFRINYFNTEIFNKENDVNLLVKKLEKMLTNFGISSKN
SINLIEYLNYLLALLPQKKG

Specific function: This methylase recognizes the double-stranded sequence GGTACC, causes specific methylation on A-4 on both strands, and protects the DNA from cleavage by the KpnI endonuclease [H]

COG id: COG2189

COG function: function code L; Adenine specific DNA methylase Mod

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002295
- InterPro:   IPR002941
- InterPro:   IPR002052 [H]

Pfam domain/function: PF01555 N6_N4_Mtase [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 48722; Mature: 48722

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNERLKLARDLLKEDGIIFVSIDDNQQAYLKVLMDEIFGEENFVANFPFISNLSGRQVNT
CCCHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCEECC
NLALCHEYLLLYKKNNFIFNKIDKDYANNFMPIVYPERSNVIFNEDPDVNKEIYDNSPEQ
HHHHHHHHHHEEECCCEEEECCCHHHHCCCEEEEEECCCCEEECCCCCCCHHHCCCCCCC
KEDNSTQYILRDNLENSNGEFNPKTRPNLFFPIYARKINEKNKLDITVEKPDSKHDFIEI
CCCCCCEEEEECCCCCCCCCCCCCCCCCEEEEEHHHHCCCCCCEEEEEECCCCCCCEEEC
PRKNSKNIQLVWRGKKKIMNQINDLVIVEPRDKNDKNYRIKVKIFDQSYTVKSFILGKKF
CCCCCCCEEEEECCHHHHHHHCCCEEEECCCCCCCCCEEEEEEEECCCCEEEHHHCCCCC
NNKSATKELNKIFSESSEKVFNFPKSTALIEYLINLHPNKNARVLDFFAGSGTTGQAVLE
CCCHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEEE
LNSQDGGTRSFTLITNNQNKIAENVTYERLFRINHGFGTKKEQNFDWIKKNQPYKTNLDV
EECCCCCCEEEEEEECCCHHHHHHCCHHHHEEEECCCCCCCCCCCCHHHCCCCCCCCCEE
FRINYFNTEIFNKENDVNLLVKKLEKMLTNFGISSKNSINLIEYLNYLLALLPQKKG
EEEEEECCEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MNERLKLARDLLKEDGIIFVSIDDNQQAYLKVLMDEIFGEENFVANFPFISNLSGRQVNT
CCCHHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHCCCCCEEECCCCCCCCCCCEECC
NLALCHEYLLLYKKNNFIFNKIDKDYANNFMPIVYPERSNVIFNEDPDVNKEIYDNSPEQ
HHHHHHHHHHEEECCCEEEECCCHHHHCCCEEEEEECCCCEEECCCCCCCHHHCCCCCCC
KEDNSTQYILRDNLENSNGEFNPKTRPNLFFPIYARKINEKNKLDITVEKPDSKHDFIEI
CCCCCCEEEEECCCCCCCCCCCCCCCCCEEEEEHHHHCCCCCCEEEEEECCCCCCCEEEC
PRKNSKNIQLVWRGKKKIMNQINDLVIVEPRDKNDKNYRIKVKIFDQSYTVKSFILGKKF
CCCCCCCEEEEECCHHHHHHHCCCEEEECCCCCCCCCEEEEEEEECCCCEEEHHHCCCCC
NNKSATKELNKIFSESSEKVFNFPKSTALIEYLINLHPNKNARVLDFFAGSGTTGQAVLE
CCCHHHHHHHHHHHCCCHHHHCCCCHHHHHHHHHHCCCCCCCEEEEEECCCCCCCEEEEE
LNSQDGGTRSFTLITNNQNKIAENVTYERLFRINHGFGTKKEQNFDWIKKNQPYKTNLDV
EECCCCCCEEEEEEECCCHHHHHHCCHHHHEEEECCCCCCCCCCCCHHHCCCCCCCCCEE
FRINYFNTEIFNKENDVNLLVKKLEKMLTNFGISSKNSINLIEYLNYLLALLPQKKG
EEEEEECCEEECCCCCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1754388 [H]