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The map label for this gene is clpB

Identifier: 54020123

GI number: 54020123

Start: 310541

End: 312619

Strand: Direct

Name: clpB

Synonym: mhp278

Alternate gene names: 54020123

Gene position: 310541-312619 (Clockwise)

Preceding gene: 54020122

Following gene: 54020124

Centisome position: 34.78

GC content: 30.3

Gene sequence:

>2079_bases
ATGAAACAAAAACTCGAAAATCTTGAAAAATACGCAAGAAATTTAACAAAATTAGCAAAAGAAAATAAAATTGAACCTGT
TATCGGCCGCGATGACGAAATTAGAAGGATTATCAAAATTTTATCCCGAAAAACAAAAAATAATCCTGTCCTTGTCGGGG
AACCTGGAGTGGGTAAGACTGCAATTGTTGAAGGAATGGCTCTAAAGATTATTGCAAATCAAGTACCGGATAATTTAAAA
AATAAACAAATTTTTGAATTAGATCTAACCTTAATTCTTGCAGGTGCTTCTTTTAAAGGTGAATTCGAAAAACGAATTAA
GGCAATTTTGCAAGAAATTGAGCAAAATTCCGATCAAGTTATTATTTTTATTGATGAAATTCACCTTCTAATTGGAACAG
GATCTTCTGGGACTGATTCAATGGATTTTGCCAATATCCTAAAACCAATTATGGCTCGCGGACAGATTAAATTAATCGGG
GCTACCACAAATTCCGAATATCGCTTATATATCGAAAAAGATGGCGCCCTTGAAAGAAGAATGCAAAAAGTAGAAATTTT
AGAGCCTTCAGTTATTGATACAATTAATATTTTACGGGGAATTAAGGAAAGGCTAGAAAATTTCCATCAAGTAAAAATTA
AGGATTCTGCTCTTGTTTTTGCTACAAAAGCGGCAAATCGTTACATTTTTGACCGCTTTCTACCTGATAAAGCTATCGAT
TTAGTCGATGAAGCTGCTGCTTCTTTAAAAGTTGAAATCAACTACCAACCAGAAAAACTTGAAAAAGCAAAGCGCGAGCT
AATTTATTTAAAAATGGAAGAAATTAACTCGCAAAAACAAGATAATTCAGAATTAAAATCCAAAATTGAAAATCTTGAAA
ATGAAGTAAAAAAATTACAAGATCAATGGGATCAATCAAAAAAATCAGCCTCTGAAATCGCTAGCTTATCCCAGGAACTT
GAAAAACTAAAATATCAACAAAATTACTTAATGGAACAAGGAGACTACCAAAAAGCCGCCGAGATTAAATACGGAAAAAT
TCCCAAAATAAGTAAAAAATTAGGCGAAATTAAAGCAAGAAAGCAAGAAATTTCCAATGTTTTAGACGAAAGTCAGATCG
CAAAGGTTGTCTCTAATTGAACAAAAATTCCGATTGAAAAACTTTTAGAATCAGAAATTCAAAAATATCTGAATTTAGAA
AAAAATTTAGCAAAATCGCTTAAGGGTCAAAATCAGGCAATTAAGGCTGTTTCAGATGCGATTTTGCGGTTTAAAGCTAA
AATTAATGATGAATCGCGCCCAATTTCATCATTTTTCTTTATGGGACCAACTGGGGTGGGAAAAACTGAACTTGCTAGAG
CTTTAGCTCTTAATTTATTTAATAATAAAAACCAAATAATCCGTCTTGATATGTCAGAATATATGGAAAAACATAGTGTT
TCAAAGCTAATTGGGGCTCCTCCGGGTTATATTGGTTTTGAACAAGGTGGTAATCTAACAAATAAAGTAAGACTAAATCC
TTATTCGATTATTTTGCTTGATGAAATTGAAAAAGCTCATCCGGAAGTAATCAACATTTTTTTACAAATTCTTGATAATG
GTGAAATTGTTGATAGTAAGTCACAAAAAGTAAATTTTCGCAATACAATTATAATTATGACCTCAAATATCGGTGCTAAT
AAAATTCTTGAGGGTAAAAAGATGAATGAAATTGAGGCAAAAAAGGAACTTTTAAGATATTTAAAGCCAGAATTTCTCAA
CCGAATTGATGAAATTATCGTATTTAATCCTTTAAATTATGATATAATTTTTGAAATTATTGAACTTGAACTAAAGGATT
TGCAAAATCGTCTAAAGGAAAATAATTTTGAGATTGAATTTGAAAAATCAGTCAAAAATTGAATTTTAGAGTTTGGATAT
GATAAAAATTTTGGTGCCAGGCCAATTAAACGCTTTATTAAGAAAGAAATTGAAAATTTTGTTGCCAAAAAAATAGTGGC
CGAAGAAATTTTAAAAGATAAAAAATACAATTTATCCTTTAAAAATGATAAATTGTATCTTAATGAAAGCGAAAATTAA

Upstream 100 bases:

>100_bases
TTCATTTAAGAAAAAAAATTGGCACTCTTTACTTTTAAGTGCCAAAAAATATGTTATAATTTATTTTGTTAAGAAAAATA
AAAAATTAAAAAGGAAAAAA

Downstream 100 bases:

>100_bases
CCTTTTATTTTAAAGAATTTTCCAAAGCTGTTCTAAAAGTTTGCTTATCCTCAAACTGATAGTCAAATTGTGTGAAAAAT
TTAAACAAAACCTCGGTATT

Product: ATP-dependent protease binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 692; Mature: 692

Protein sequence:

>692_residues
MKQKLENLEKYARNLTKLAKENKIEPVIGRDDEIRRIIKILSRKTKNNPVLVGEPGVGKTAIVEGMALKIIANQVPDNLK
NKQIFELDLTLILAGASFKGEFEKRIKAILQEIEQNSDQVIIFIDEIHLLIGTGSSGTDSMDFANILKPIMARGQIKLIG
ATTNSEYRLYIEKDGALERRMQKVEILEPSVIDTINILRGIKERLENFHQVKIKDSALVFATKAANRYIFDRFLPDKAID
LVDEAAASLKVEINYQPEKLEKAKRELIYLKMEEINSQKQDNSELKSKIENLENEVKKLQDQWDQSKKSASEIASLSQEL
EKLKYQQNYLMEQGDYQKAAEIKYGKIPKISKKLGEIKARKQEISNVLDESQIAKVVSNWTKIPIEKLLESEIQKYLNLE
KNLAKSLKGQNQAIKAVSDAILRFKAKINDESRPISSFFFMGPTGVGKTELARALALNLFNNKNQIIRLDMSEYMEKHSV
SKLIGAPPGYIGFEQGGNLTNKVRLNPYSIILLDEIEKAHPEVINIFLQILDNGEIVDSKSQKVNFRNTIIIMTSNIGAN
KILEGKKMNEIEAKKELLRYLKPEFLNRIDEIIVFNPLNYDIIFEIIELELKDLQNRLKENNFEIEFEKSVKNWILEFGY
DKNFGARPIKRFIKKEIENFVAKKIVAEEILKDKKYNLSFKNDKLYLNESEN

Sequences:

>Translated_692_residues
MKQKLENLEKYARNLTKLAKENKIEPVIGRDDEIRRIIKILSRKTKNNPVLVGEPGVGKTAIVEGMALKIIANQVPDNLK
NKQIFELDLTLILAGASFKGEFEKRIKAILQEIEQNSDQVIIFIDEIHLLIGTGSSGTDSMDFANILKPIMARGQIKLIG
ATTNSEYRLYIEKDGALERRMQKVEILEPSVIDTINILRGIKERLENFHQVKIKDSALVFATKAANRYIFDRFLPDKAID
LVDEAAASLKVEINYQPEKLEKAKRELIYLKMEEINSQKQDNSELKSKIENLENEVKKLQDQWDQSKKSASEIASLSQEL
EKLKYQQNYLMEQGDYQKAAEIKYGKIPKISKKLGEIKARKQEISNVLDESQIAKVVSN*TKIPIEKLLESEIQKYLNLE
KNLAKSLKGQNQAIKAVSDAILRFKAKINDESRPISSFFFMGPTGVGKTELARALALNLFNNKNQIIRLDMSEYMEKHSV
SKLIGAPPGYIGFEQGGNLTNKVRLNPYSIILLDEIEKAHPEVINIFLQILDNGEIVDSKSQKVNFRNTIIIMTSNIGAN
KILEGKKMNEIEAKKELLRYLKPEFLNRIDEIIVFNPLNYDIIFEIIELELKDLQNRLKENNFEIEFEKSVKN*ILEFGY
DKNFGARPIKRFIKKEIENFVAKKIVAEEILKDKKYNLSFKNDKLYLNESEN
>Mature_692_residues
MKQKLENLEKYARNLTKLAKENKIEPVIGRDDEIRRIIKILSRKTKNNPVLVGEPGVGKTAIVEGMALKIIANQVPDNLK
NKQIFELDLTLILAGASFKGEFEKRIKAILQEIEQNSDQVIIFIDEIHLLIGTGSSGTDSMDFANILKPIMARGQIKLIG
ATTNSEYRLYIEKDGALERRMQKVEILEPSVIDTINILRGIKERLENFHQVKIKDSALVFATKAANRYIFDRFLPDKAID
LVDEAAASLKVEINYQPEKLEKAKRELIYLKMEEINSQKQDNSELKSKIENLENEVKKLQDQWDQSKKSASEIASLSQEL
EKLKYQQNYLMEQGDYQKAAEIKYGKIPKISKKLGEIKARKQEISNVLDESQIAKVVSN*TKIPIEKLLESEIQKYLNLE
KNLAKSLKGQNQAIKAVSDAILRFKAKINDESRPISSFFFMGPTGVGKTELARALALNLFNNKNQIIRLDMSEYMEKHSV
SKLIGAPPGYIGFEQGGNLTNKVRLNPYSIILLDEIEKAHPEVINIFLQILDNGEIVDSKSQKVNFRNTIIIMTSNIGAN
KILEGKKMNEIEAKKELLRYLKPEFLNRIDEIIVFNPLNYDIIFEIIELELKDLQNRLKENNFEIEFEKSVKN*ILEFGY
DKNFGARPIKRFIKKEIENFVAKKIVAEEILKDKKYNLSFKNDKLYLNESEN

Specific function: Part of a stress-induced multi-chaperone system, it is involved in the recovery of the cell from heat-induced damage, in cooperation with DnaK, DnaJ and GrpE. Acts before DnaK, in the processing of protein aggregates. Protein binding stimulates the ATPase

COG id: COG0542

COG function: function code O; ATPases with chaperone activity, ATP-binding subunit

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the clpA/clpB family [H]

Homologues:

Organism=Homo sapiens, GI13540606, Length=298, Percent_Identity=34.2281879194631, Blast_Score=184, Evalue=3e-46,
Organism=Escherichia coli, GI1788943, Length=696, Percent_Identity=46.4080459770115, Blast_Score=652, Evalue=0.0,
Organism=Escherichia coli, GI1787109, Length=342, Percent_Identity=38.3040935672515, Blast_Score=245, Evalue=7e-66,
Organism=Saccharomyces cerevisiae, GI6320464, Length=704, Percent_Identity=44.1761363636364, Blast_Score=592, Evalue=1e-170,
Organism=Saccharomyces cerevisiae, GI6323002, Length=708, Percent_Identity=40.6779661016949, Blast_Score=539, Evalue=1e-154,

Paralogues:

None

Copy number: 560 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR013093
- InterPro:   IPR003959
- InterPro:   IPR018368
- InterPro:   IPR017730
- InterPro:   IPR001270
- InterPro:   IPR019489
- InterPro:   IPR004176
- InterPro:   IPR023150 [H]

Pfam domain/function: PF00004 AAA; PF07724 AAA_2; PF02861 Clp_N; PF10431 ClpB_D2-small [H]

EC number: NA

Molecular weight: Translated: 79092; Mature: 79092

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: PS00871 CLPAB_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKQKLENLEKYARNLTKLAKENKIEPVIGRDDEIRRIIKILSRKTKNNPVLVGEPGVGKT
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCHH
AIVEGMALKIIANQVPDNLKNKQIFELDLTLILAGASFKGEFEKRIKAILQEIEQNSDQV
HHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEE
IIFIDEIHLLIGTGSSGTDSMDFANILKPIMARGQIKLIGATTNSEYRLYIEKDGALERR
EEEEEEEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEECCCHHHHH
MQKVEILEPSVIDTINILRGIKERLENFHQVKIKDSALVFATKAANRYIFDRFLPDKAID
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEEEECCEEEEEEHHCCHHHHHHHCCHHHHH
LVDEAAASLKVEINYQPEKLEKAKRELIYLKMEEINSQKQDNSELKSKIENLENEVKKLQ
HHHHHHHEEEEEECCCHHHHHHHHHHHEEEEHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
DQWDQSKKSASEIASLSQELEKLKYQQNYLMEQGDYQKAAEIKYGKIPKISKKLGEIKAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCHHHHHHHHHHHH
KQEISNVLDESQIAKVVSNTKIPIEKLLESEIQKYLNLEKNLAKSLKGQNQAIKAVSDAI
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
LRFKAKINDESRPISSFFFMGPTGVGKTELARALALNLFNNKNQIIRLDMSEYMEKHSVS
HHHHHHCCCCCCCCHHEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHH
KLIGAPPGYIGFEQGGNLTNKVRLNPYSIILLDEIEKAHPEVINIFLQILDNGEIVDSKS
HHHCCCCCCCCCCCCCCCCCEEEECCEEEEEEHHHHHHHHHHHHHHHHHHCCCCEECCCC
QKVNFRNTIIIMTSNIGANKILEGKKMNEIEAKKELLRYLKPEFLNRIDEIIVFNPLNYD
CCEEECEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHEEEECCCCHH
IIFEIIELELKDLQNRLKENNFEIEFEKSVKNILEFGYDKNFGARPIKRFIKKEIENFVA
HHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
KKIVAEEILKDKKYNLSFKNDKLYLNESEN
HHHHHHHHHCCCCCCCEECCCEEEECCCCC
>Mature Secondary Structure
MKQKLENLEKYARNLTKLAKENKIEPVIGRDDEIRRIIKILSRKTKNNPVLVGEPGVGKT
CCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEECCCCCHH
AIVEGMALKIIANQVPDNLKNKQIFELDLTLILAGASFKGEFEKRIKAILQEIEQNSDQV
HHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEEEECCCCCHHHHHHHHHHHHHHHCCCCEE
IIFIDEIHLLIGTGSSGTDSMDFANILKPIMARGQIKLIGATTNSEYRLYIEKDGALERR
EEEEEEEEEEEECCCCCCCCHHHHHHHHHHHCCCCEEEEEEECCCCEEEEEECCCHHHHH
MQKVEILEPSVIDTINILRGIKERLENFHQVKIKDSALVFATKAANRYIFDRFLPDKAID
HHHHHHCCCHHHHHHHHHHHHHHHHHHHHEEEEECCEEEEEEHHCCHHHHHHHCCHHHHH
LVDEAAASLKVEINYQPEKLEKAKRELIYLKMEEINSQKQDNSELKSKIENLENEVKKLQ
HHHHHHHEEEEEECCCHHHHHHHHHHHEEEEHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
DQWDQSKKSASEIASLSQELEKLKYQQNYLMEQGDYQKAAEIKYGKIPKISKKLGEIKAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCHHHHHHHHHHHH
KQEISNVLDESQIAKVVSNTKIPIEKLLESEIQKYLNLEKNLAKSLKGQNQAIKAVSDAI
HHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHH
LRFKAKINDESRPISSFFFMGPTGVGKTELARALALNLFNNKNQIIRLDMSEYMEKHSVS
HHHHHHCCCCCCCCHHEEEECCCCCCHHHHHHHHHHHHHCCCCCEEEECHHHHHHHHHHH
KLIGAPPGYIGFEQGGNLTNKVRLNPYSIILLDEIEKAHPEVINIFLQILDNGEIVDSKS
HHHCCCCCCCCCCCCCCCCCEEEECCEEEEEEHHHHHHHHHHHHHHHHHHCCCCEECCCC
QKVNFRNTIIIMTSNIGANKILEGKKMNEIEAKKELLRYLKPEFLNRIDEIIVFNPLNYD
CCEEECEEEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHCHHHHHHHHHEEEECCCCHH
IIFEIIELELKDLQNRLKENNFEIEFEKSVKNILEFGYDKNFGARPIKRFIKKEIENFVA
HHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHH
KKIVAEEILKDKKYNLSFKNDKLYLNESEN
HHHHHHHHHCCCCCCCEECCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Hydrolase; Acting on peptide bonds (Peptidases); Serine endopeptidases [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12240834 [H]