Click here to switch to the map view.

The map label for this gene is rsmE [H]

Identifier: 54020067

GI number: 54020067

Start: 220345

End: 221028

Strand: Direct

Name: rsmE [H]

Synonym: mhp181

Alternate gene names: 54020067

Gene position: 220345-221028 (Clockwise)

Preceding gene: 54020066

Following gene: 54020068

Centisome position: 24.68

GC content: 28.65

Gene sequence:

>684_bases
ATGTATCGCTTTTTTGTCAATGAAAAACAAGAAAATTTCTTTATCTTAACAAATCTAACCTTAAATCATATTAAAACTGT
AAGAATTAAAAATGAAAATTTTATTTGTGTTTATCAAAATGAATTCTACCTTGTGAGATTAGTCCCTAATTCAAACAAAG
CAGAAATAATTGAAAAAATTAAGGGGAATAACGAACCAAAAAACAAAGTTGTTCTCGCACTTGCAATTTTGAAGACAAAA
TCATTTGAATTTGCAATCCAGAAAGCTGTTGAAATTGGGGTTAATGAAATTTGACCTTTTTATAGTAAAAATGTTTCCCA
AAAACTAAGCGGGGATCTTGAAAAAAAACTCAAAAGATGAGAACAAATTTGTCTTCATAGTGCCCAACAAAGTTTCCGCA
ATTTGATTCCAAAAATAAATTTACCTATAAATTATAAGGATCTTTTAGAACAAGCCAAAAATTTTCCAGTAAAATTAATC
AGTTTTGAAAGAGCAAAAAACAATATAAATATCCCTGATAACCCCCAAAATACAATTATAATTATTGGGCCTGAAGGTGG
GTTTGATGATGTTGAAATCCAACAAGCAGAAAAATTAGGGTTTCAAAGTATCACACTCGGGAAAAGAATTCTTAGATCTG
AGACAGCCGCAATTTTTCTTTTAACAAAATGTATAAAAGATTAA

Upstream 100 bases:

>100_bases
ATTTCGTTTGCTGATAATTTTTCCATAGTTTTTCCTTCTTTTTTAATAATTTTAAACTATTTTTTAAATAGTTTATCAAT
TTTCCTTTAAAATTTTTATT

Downstream 100 bases:

>100_bases
TTTTGGCTAGAAATGCAAATTTTTATAAAAATTTGCATTATTAAACATCCTTTTTCAACGAAAAAATAGATTTAATAATA
TATCACCAATATTATCAAAA

Product: 16S ribosomal RNA methyltransferase RsmE

Products: NA

Alternate protein names: 16S rRNA m3U1498 methyltransferase [H]

Number of amino acids: Translated: 227; Mature: 227

Protein sequence:

>227_residues
MYRFFVNEKQENFFILTNLTLNHIKTVRIKNENFICVYQNEFYLVRLVPNSNKAEIIEKIKGNNEPKNKVVLALAILKTK
SFEFAIQKAVEIGVNEIWPFYSKNVSQKLSGDLEKKLKRWEQICLHSAQQSFRNLIPKINLPINYKDLLEQAKNFPVKLI
SFERAKNNINIPDNPQNTIIIIGPEGGFDDVEIQQAEKLGFQSITLGKRILRSETAAIFLLTKCIKD

Sequences:

>Translated_227_residues
MYRFFVNEKQENFFILTNLTLNHIKTVRIKNENFICVYQNEFYLVRLVPNSNKAEIIEKIKGNNEPKNKVVLALAILKTK
SFEFAIQKAVEIGVNEI*PFYSKNVSQKLSGDLEKKLKR*EQICLHSAQQSFRNLIPKINLPINYKDLLEQAKNFPVKLI
SFERAKNNINIPDNPQNTIIIIGPEGGFDDVEIQQAEKLGFQSITLGKRILRSETAAIFLLTKCIKD
>Mature_227_residues
MYRFFVNEKQENFFILTNLTLNHIKTVRIKNENFICVYQNEFYLVRLVPNSNKAEIIEKIKGNNEPKNKVVLALAILKTK
SFEFAIQKAVEIGVNEI*PFYSKNVSQKLSGDLEKKLKR*EQICLHSAQQSFRNLIPKINLPINYKDLLEQAKNFPVKLI
SFERAKNNINIPDNPQNTIIIIGPEGGFDDVEIQQAEKLGFQSITLGKRILRSETAAIFLLTKCIKD

Specific function: Specifically methylates the uridine in position 1498 of 16S rRNA in the fully assembled 30S ribosomal subunit [H]

COG id: COG1385

COG function: function code S; Uncharacterized protein conserved in bacteria

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase RsmE family [H]

Homologues:

Organism=Escherichia coli, GI87082186, Length=209, Percent_Identity=29.1866028708134, Blast_Score=78, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006700 [H]

Pfam domain/function: PF04452 Methyltrans_RNA [H]

EC number: =2.1.1.193 [H]

Molecular weight: Translated: 25878; Mature: 25878

Theoretical pI: Translated: 9.95; Mature: 9.95

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYRFFVNEKQENFFILTNLTLNHIKTVRIKNENFICVYQNEFYLVRLVPNSNKAEIIEKI
CEEEEECCCCCCEEEEEECEECCEEEEEECCCCEEEEEECCEEEEEECCCCCHHHHHHHH
KGNNEPKNKVVLALAILKTKSFEFAIQKAVEIGVNEIPFYSKNVSQKLSGDLEKKLKREQ
CCCCCCCHHEEEEEEEHHCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHCCHHHHHHHHHH
ICLHSAQQSFRNLIPKINLPINYKDLLEQAKNFPVKLISFERAKNNINIPDNPQNTIIII
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCEEEEE
GPEGGFDDVEIQQAEKLGFQSITLGKRILRSETAAIFLLTKCIKD
CCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MYRFFVNEKQENFFILTNLTLNHIKTVRIKNENFICVYQNEFYLVRLVPNSNKAEIIEKI
CEEEEECCCCCCEEEEEECEECCEEEEEECCCCEEEEEECCEEEEEECCCCCHHHHHHHH
KGNNEPKNKVVLALAILKTKSFEFAIQKAVEIGVNEIPFYSKNVSQKLSGDLEKKLKREQ
CCCCCCCHHEEEEEEEHHCCCHHHHHHHHHHCCCCCCCCCCCCHHHHHCCHHHHHHHHHH
ICLHSAQQSFRNLIPKINLPINYKDLLEQAKNFPVKLISFERAKNNINIPDNPQNTIIII
HHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHCCCEEEEEEECCCCCCCCCCCCCCEEEEE
GPEGGFDDVEIQQAEKLGFQSITLGKRILRSETAAIFLLTKCIKD
CCCCCCCCCHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9729120 [H]